Karte des noerdlichen Ural und des Kuesten-Gebirges Pae-Choi : entworfen nach Aufnahmen und astronomischen Ortsbestimmungen, ausführt auf der ... Ural-Expedition in den Jahren 1847,1848 und 1850

Kartta kuuluu A. E. Nordenskiöldin kokoelmaan

[Karte des noerdlichen Ural und des Kuesten-Gebirges Pae-Choi : entworfen nach Aufnahmen und astronomischen Ortsbestimmungen, ausführt auf der ... Ural-Expedition in den Jahren 1847,1848 und 1850]

Kartta kuuluu A. E. Nordenskiöldin kokoelmaan

Secretory TAT-peptide-mediated protein transduction of LIF receptor α-chain distal cytoplasmic motifs into human myeloid HL-60 cells

The distal cytoplasmic motifs of leukemia inhibitory factor receptor α-chain (LIFRα-CT3) can independently induce intracellular myeloid differentiation in acute myeloid leukemia (AML) cells by gene transfection; however, there are significant limitations in the potential clinical use of these motifs due to liposome-derived genetic modifications. To produce a potentially therapeutic LIFRα-CT3 with cell-permeable activity, we constructed a eukaryotic expression pcDNA3.0-TAT-CT3-cMyc plasmid with a signal peptide (ss) inserted into the N-terminal that codes for an ss-TAT-CT3-cMyc fusion protein. The stable transfection of Chinese hamster ovary (CHO) cells via this vector and subsequent selection by Geneticin resulted in cell lines that express and secrete TAT-CT3-cMyc. The spent medium of pcDNA3.0-TAT-CT3-cMyc-transfected CHO cells could be purified using a cMyc-epitope-tag agarose affinity chromatography column and could be detected via SDS-PAGE, with antibodies against cMyc-tag. The direct administration of TAT-CT3-cMyc to HL-60 cell culture media caused the enrichment of CT3-cMyc in the cytoplasm and nucleus within 30 min and led to a significant reduction of viable cells (P < 0.05) 8 h after exposure. The advantages of using this mammalian expression system include the ease of generating TAT fusion proteins that are adequately transcripted and the potential for a sustained production of such proteins in vitro for future AML therapy.

Bioinformatics analysis of biomarkers and transcriptional factor motifs in Down syndrome

In this study, biomarkers and transcriptional factor motifs were identified in order to investigate the etiology and phenotypic severity of Down syndrome. GSE 1281, GSE 1611, and GSE 5390 were downloaded from the gene expression ominibus (GEO). A robust multiarray analysis (RMA) algorithm was applied to detect differentially expressed genes (DEGs). In order to screen for biological pathways and to interrogate the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database, the database for annotation, visualization, and integrated discovery (DAVID) was used to carry out a gene ontology (GO) function enrichment for DEGs. Finally, a transcriptional regulatory network was constructed, and a hypergeometric distribution test was applied to select for significantly enriched transcriptional factor motifs. CBR1, DYRK1A, HMGN1, ITSN1, RCAN1, SON, TMEM50B, and TTC3 were each up-regulated two-fold in Down syndrome samples compared to normal samples; of these, SON and TTC3 were newly reported. CBR1, DYRK1A, HMGN1, ITSN1, RCAN1, SON, TMEM50B, and TTC3 were located on human chromosome 21 (mouse chromosome 16). The DEGs were significantly enriched in macromolecular complex subunit organization and focal adhesion pathways. Eleven significantly enriched transcription factor motifs (PAX5, EGR1, XBP1, SREBP1, OLF1, MZF1, NFY, NFKAPPAB, MYCMAX, NFE2, and RP58) were identified. The DEGs and transcription factor motifs identified in our study provide biomarkers for the understanding of Down syndrome pathogenesis and progression.

Förteckning öfver afl. provincial-läkaren d:r A. J. Orrströms efterlemnade böcker af medicinskt innehåll, hvilka försäljas å offentlig frivillig auction i Helsingfors år 1848 på de i tidningarne bestämmande bokauctionsdagar

Imprimatur: N. A. Gyldén.

Förteckning öfver aflidne provincial-läkaren dr. A. J. Orrströms efterlemnade boksamling af bellettristiskt och annat icke-medicinskt innehåll, att föryttras å frivillig auction på Auctionskammaren i Helsingfors år 1848

Imprimatur: G. Rein.

Förteckning öfver böcker och chartor m. m. som komma att försäljas på Auctionskammaren i Helsingfors den 23 febr. 1848 och följande bokauctions dagar

Imprimatur: G. Rein.

Förteckning öfver böcker, som genom auktion komma att försäljas, i Helsingfors den [ ] martii 1848 o. ff. dagar

Imprimatur: G. Rein.

La Montagne (Paris. 1848)

Périodicité : Mensuel

La Révolution française : revue historique...

1894/01 (T26)-1894/06.

La Révolution française : revue historique...

1894/07 (T27)-1894/12.