Schematicheskai︠a︡ karta chasti territorii Zaporozhskogo i Mariupolʻskogo okrugov s ukazaniem evreǐskikh zemelʻpolʻzovaniǐ i gosfondov v blizi nikh

Digital image

Obezpechennostʻ zhivym inventarem, obsluzhivaemykh "Agro-dzhoǐntom" pereselent︠s︡ev Krivorogskogo raǐona, osevshikh na zemli︠u︡ v raznye sroki po dannym na 1oe i︠u︡li︠a︡ 1926 goda

Digital image

Tipovai︠a︡ usadʻba s posazhenymi plodovymi derevʻi︠a︡mi tirinoi︠u︡ 20 sazhenʻ

Digital image

Obezpechennostʻ srednego pereselencheskogo khozi︠a︡ǐstva Krivorogskogo okruga raǐona dei︠a︡telʻnosti "Agro-dzhoǐnta" po vremeni poselenii︠a︡

Digital image

Plan zemelʻnogo otvoda uchastka N 50-51 poselka N 2 Krivorogskogo okruga Shirokovskogo raǐona ploshadʻi︠u︡ 557,0720 gektara

Digital image

Plan zemelʻnogo otvoda uchastka N 50-51 poselka N 1 Krivorogskogo okruga Shirokovskogo raǐona ploshadʻi︠u︡ 592.2279 gektara

Digital image

Plan zemelʻnogo otvoda uchastka lit. "K" kollektivov "Kalininovka" i "Novai︠a︡ Zhiznʻ" Krivorogskogo okruga Sofievskogo raĭona ploshchadiʻ︠u︡ 569 desi︠a︡tin

Digital image

Characterization of Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease, reveals a unique mode of DNA-binding, helical distortion and cleavage, compared to a canonical LAGLIDADG homing endonuclease

Mycobacterium leprae, which has undergone reductive evolution leaving behind a minimal set of essential genes, has retained intervening sequences in four of its genes implicating a vital role for them in the survival of the leprosy bacillus. A single in-frame intervening sequence has been found embedded within its recA gene. Comparison of M. leprae recA intervening sequence with the known intervening sequences indicated that it has the consensus amino acid sequence necessary for being a LAGLIDADG-type homing endonuclease. In light of massive gene decay and function loss in the leprosy bacillus, we sought to investigate whether its recA intervening sequence encodes a catalytically active homing endonuclease. Here we show that the purified M. leprae RecA intein (PI-MleI) binds to cognate DNA and displays endonuclease activity in the presence of alternative divalent cations, Mg2+ or Mn2+. A combination of approaches including four complementary footprinting assays such as DNase I, Cu/phenanthroline, methylation protection and KMnO4, enhancement of 2-aminopurine fluorescence and mapping of the cleavage site revealed that PI-MleI binds to cognate DNA flanking its insertion site, induces helical distortion at the cleavage site and generates two staggered double-strand breaks. Taken together, these results implicate that PI-MleI possess a modular structure with separate domains for DNA target recognition and cleavage, each with distinct sequence preferences. From a biological standpoint, it is tempting to speculate that our findings have implications for understanding the evolution of LAGLIDADG family of homing endonucleases

Plan uchastka gosfonda kollektiva "Trud" Krivorogskogo okruga i raĭona ploshchadiʻ︠u︡ 107 desi︠a︡tin

Digital image

Plan zemelʻnogo otvoda kollektiva "Khlib i Prat︠s︡i︠a︡" Krivorogskogo okruga Mikhaĭlovskogo raĭona

Digital image

Plan raspolozhenii︠a︡ poselka i domov kolektiva "Novyǐ Putʻ"

Digital image

Plan zemelʻnogo otvoda kolektiva "Khlib i Prat︠s︡i︠a︡" Krivorogskogo okruga Mikhaĭlovskogo raĭona

Digital image

Plan uchastka kolfonda litera "I" Mikhaĭlovskogo raĭona Krivorogskogo okruga ploshchadiʻ︠u︡ 458 desi︠a︡tin

Digital image

Plan zemelʻnogo otvoda uchastka litera "K" kollektivov "Kalininovka" i "Novai︠a︡ Zhiznʻ" Krivorogskogo okruga Sofievskogo raĭona ploshchadiʻ︠u︡ 569 desi︠a︡tin

Digital image

Plan uchastka kolonizat︠s︡ionnogo fonda pod literami "A" i "B" Krivorogskogo okruga Mikhaĭlovskogo raĭona ploshchadiʻ︠u︡ 1356 desi︠a︡tin

Digital image