O Programa de Aquisição de Alimentos (PAA) e a disputa territorial em torno da luta pela/na terra no Pontal do Paranapanema

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Qualidade microbiológica de lanches e salgados comercializados em Botucatu, SP

A entrada das mulheres no mercado de trabalho, a dificuldade de locomoção do local de trabalho até a residência, a falta de tempo para o preparo das refeições e o preço mais acessível fez com que a escolha por lanches e refeições rápidas tenha crescido muito, nos últimos anos. Entretanto, esses alimentos estão sujeitos a contaminação desde seu preparo até o momento do consumo, além da manutenção em temperaturas inadequadas. Assim, o objetivo do trabalho foi avaliar microbiológica lanches e salgados comercializados em lanchonetes, “lojas de conveniência” e trailers das principais áreas da cidade de Botucatu, deacordo com os padrões estabelecidos pela ANVISA (RDC N º 12, 2001). As análises foram realizadas de acordo com o Compendium (APHA, 2001). Foram analisadas 122 amostras de salgados de carne, coxinhas, risoles de queijo, camarão e palmito, enrolado de salsicha além de salgado de frios e sanduíches, das quais 17 (13,9%) excederam aos limites microbiológicos. Excesso de coliformes termotolerantes foram encontrados 2 coxinhas, 2 risoles de palmito e 6 salgados de carne, no total de 10 amostras (8,2%). A presença Salmonella spp. foi detectada em duas amostras (1,6%) e clostrídios sulfito redutores apresentaram concentrações acima dos limites em somente uma (0,8%) amostra de salgado de carne. Não foram encontradas amostras com excesso de Bacillus cereus. Staphyloccocus aureus foi observado em valores acima dos permitidos em 8 amostras (6,6%) (5 coxinhas, 2 risoles de frios e 1 salgado). A partir das amostras analisadas, os resultados obtidos indicam que a maioria dos salgados estava dentro dos limites microbiológicos estabelecidos pela ANVISA (2001). Entretanto, 9% das amostras apresentaram patógenos, expondo a população a riscos. É necessária maior vigilância das autoridades competentes e, principalmente, conscientização por parte dos vendedores

Qualidade microbiológica de peixes e frutos do mar em Botucatu, SP

whole world. The world production of fish is around 100 million tons/year and 70% of them is destined exclusively to the human feeding. The fish and derived have a great importance in the human diet, contributing with ¼ of offer of protein of animal origin. Sseafood and fish are health, because they are rich in proteins, vitamins, micronutrients and insatured fats. However, one of the most important themes, in public health, refers to the safe food. Only in the USA, 76 million case of foodborne diseases occurs every year, with 325 thousand hospitalized and 5 thousand deaths. According to the microbiological parameters, the Brazilian Food Sanitation Standard (Decree No. 12, 2001) defined that fish, roes of fish, crustaceans and mollusks in natura cooled or frozen, no consumed raw should present up to 103 MPN of coagulase positive Staphylococcus in the absence of Salmonella in 25g. Based on this, this work verified the microbiological quality of fish and seafood retailed in supermarkets and fish store in Botucatu city. A hundred samples were analyzed, being 65 frozen (65%) and 35 cooled (35%). The samples included various kinds of fish and seafood. Among the frozen samples, 31 were fish and 34 seafood. About the cooled ones, 28 were fish and 7 seafood. The methods used for analysis are in agreement with APHA (2001). The samples were acquired in 4 establishments (3 supermarkets and a fish store). Ina total of 100 samples, all were negative to coagulase positive Staphylococcus and 2 were positive for Salmonella (2%). According to the results, we conclude that the presence of Salmonella is a risk factor for the consumers' health and these foods should not be consumed raw. In spite of the freezing to be a good conservation way, this process doesn't totally eliminate the pathogens of the food

Avaliação da vulnerabilidade a contaminação das águas subterrâneas do aquífero Rio Claro na mineração Mandu, distrito de Ajapi - Rio Claro-SP

Groundwater in the Rio Claro district is predominantly extracted from low productivity wells on the Itararé Aquifer, from depths greater than 150 m. These factors in conjunction with increasing demand for water supply have stimulated many industries to exploit the Rio Claro Aquifer, constituted by Cenozoic sediments of the Rio Claro Formation, where hydraulic properties are poorly known. For this reason, it is necessary to study the possible vulnerable to contamination punctual locations of the aquifer, in order to enable proper management of these groundwater reserves and to allow continuity of supply. Thus, this study aims to assess the vulnerability to groundwater on contamination Rio Claro Aquifer, punctually in Mandu mining site area, located in the District of Ajapi, Rio Claro - SP, the company which operates and sells industrial sand. The risk of groundwater contamination depends on the relationship between the hydrogeological characteristics of the aquifer layer (vulnerability to contamination) and the pollutant load. To evaluate the susceptibility to contamination it was applied the method GOD (FOSTER and HIRATA, 1987; FOSTER et. al., 2002) and, in addition, field and laboratory tests were performed to chacacterize the granulometry of the strata, hydraulic conductivity and some physical indices to make the evaluation more assertive and detailed. Punctual contamination vulnerability found in the aquifer Rio Claro, was moderate, which means that the aquifer is vulnerable to some contaminants, but when continuously discharged or leached. Thus, Mandu mining has a great role on preventing the emission of contaminants in the process of extraction and processing of sand... (Complete abstract click electronic access below)

Contaminação radioativa: aspectos fundamentais associados à poluição nuclear

Since its discovery, radioactivity has brought numerous benefits to human societies. It has many applications in medicine, serving as a tool for non-invasive methods for diagnosis and therapies against diseases such as cancer. It also applies to technologies for energy in nuclear power plants with relatively low impacts on terms of perfect security. All applications, however, have risks, requiring maximum caution to drive processes and operations involving radioactive elements because, once released into the environment, they have extremely harmful effects on organisms affected. This paper presents fundamental concepts and principles of nuclear physics in order to understand the effects of radioactive elements released into the environment, culminating on the issue of radioactive contamination. Literature review allowed us to understand the radioactive contamination problem on living beings. Three major nuclear accidents have happened in the last thirty years, two of them in consecutive years. The nuclear accident at Chernobyl, Ukraine, in 1986, polluted large areas, condemning hundreds of thousands of people to live with consequences of the accident and effects of radiation, killing thousands of people throughout the years. In 1987, a major radiological accident occurred in Goiania (GO) when a source of radioactive cesium was violated, leading to the death of those who had direct or indirect contact with cesium. The most recent accident, in March, 2011, was located at the nuclear power plant in Fukushima Prefecture, Japan, after an earthquake and tsunami hit the region. There is no extensive and accurate knowledge about the consequences of the contamination entailed in that accident, although it is possible to verify signals on a global scale. An analysis of reports of contamination of large areas generated by nuclear plants with release of hazardous wastes suggests it is necessary to rethink the energy matrix of the various countries...

Proliferação e Morte Celular (Apoptose) avaliadas em ratos Lewis após tratamento com mistura de praguicidas em baixas doses

The use of pesticides in Brazil has intensified over the years. And since 2009 it was ranked as the largest market for pesticides worldwide. The consequential diffuse contamination of the environment is therefore due to the increasing number of chemicals of different classes, origins and modes of action. Little is known about the action of pesticides on human health in situations of co-exposure. Possible toxic effects are not restricted to agricultural and industrial workers, but also the general population that may be exposed continuously to its residues in food and water. Although these pesticides are mostly present in the environment at low doses, it must be considered that possible cumulative or synergistic effects may occur when there are concurrent or sustained exposure for two or more of these agents, which can lead to late manifestation of subclinical damages, sometimes irreversible. Thus, the specific objective of this study was to assess the effect of carcinogenesis promotion of a mixture of pesticides at low doses and analyze the phenomena of cell proliferation and apoptosis in rat liver. A total of 50 male Lewis rats was separated into 5 groups for 8 weeks in a medium term hepatocarcinogenesis model. The three different classes of pesticides (dieldrin, dicofol, endosulfan, dichlorvos and permethrin), whose residues were detected by ANVISA during the period from 2001 to 2005 in tomatoes cultures, were added to the feed of rats initiated to hepatocarcinogenesis with diethylnitrosamine (DEN- 200mg/kg ip). We used two different mixtures, one with no toxic effects at doses (MEX1) referring to the NOEL (no-observed-effect level) and another at doses LOEL / LEL / LOAEL (Lowest-observed-effect level / Lowest-effect level / Lowest -observed-adverse-effect level), to the installation of adverse effects (MEX2), derived from chronic studies. All animals ...(Complete abstract click electronic access below)

Mapeamento geoestatístico da permeabilidade superficial no entorno do Ribeirão Claro: e simulação da possibilidade de sua contaminação

The evaluation of soil permeability throughout the weathering profile is one of the most important features to be considered in environmental studies. This study, developed from field testing and analysis of data obtained by geostatistical methods, aims at mapping the permeability around the Ribeirão Claro river. The intent is to simulate an accident with toxic liquids where soil permeability is of fundamental importance. Another purpose of the research was to determine the minimum time that, in the event of an accident, a possible contaminant to reach the water table level and be routed to the nearest drain, in this case, the Ribeirao V Claro river constitutes fundamental information. The studied area of approximately 4 km² is located within the UNESP-Rio Claro campus, consisting of colluvial soil from Fm. Rio Claro superimposed on residual soil of Fm. Corumbataí. The method used to determine the permeability is the concentric cylinders performed on a sampling grid with 64 points containing spacing of 5 meters EW and 10 meters NS. In the places of permeability tests were collected samples for laboratory determination of the percentage of fines. From particle size analysis was performed and analysis statistical and geostatistical on this data. The histogram was based on the statistical studies, and the semivariograms were based on geostatistical estimation methods. Based on the comparison between the maps and the data obtained, it was determined that the percentage of fines in colluvial surface soil has little influence on permeability, which the proximity to the Ribeirao Claro river, the eastern portion, a factor that influences the distribution of permeability values

A cidade de Rio Claro/SP está cumprindo sua meta na compra de alimentos de agricultura familiar para o PNAE?

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O Programa de Aquisição de Alimentos e os agricultores familiares do município de Rio Claro-SP: diversidade da produção, destinação e geração de renda

A Agricultura exerce papel fundamental na economia de um país, seja ela para emprego de mão de obra e geração de renda, seja para produção de alimentos destinados para a exportação e consumo interno. No Brasil a Agricultura familiar é responsável por produzir uma parcela importante de alimentos consumidos pela população nacional. Durante a década de 1990, políticas públicas foram sendo implantadas para auxiliar o desenvolvimento rural, tendo como público alvo a agricultura familiar e incidindo sobre entraves para o seu desenvolvimento, como a questão da comercialização dos produtos advindos desse segmento. Em 2003, O Programa de Aquisição de Alimentos (PAA), foi instituído pelo artigo 19 da lei federal 10.696, que tinha como objetivo promover a inclusão social e econômica no campo por meio do fortalecimento da agricultura familiar, através da aquisição de alimentos desses produtores. Tais alimentos adquiridos são destinados a ações de alimentação empreendidas por entidades das redes sócioassistenciais, Bancos de Alimentos e para famílias em situação de vulnerabilidade social. O presente trabalho consistiu em um estudo de caso, que teve como objetivo diagnosticar a influência do PAA no município de Rio Claro (SP), quantificando o número de agricultores que participavam do programa no município, e, identificando a diversidade e quantidade de alimentos produzidos destinados ao PAA. Foram também analisados os valores destinados ao programa desde sua implantação. Além disso, foram mapeadas as entidades sócio-assistenciais que receberam os alimentos destinados ao programa. Com base na leis de nº 10.696/2003 e nº 11.326/2006, e os Decretos de nº 6.447/2008 e nº 6.959/2009, que dão providências ao PAA, foram realizadas coletas de dados e informações junto às Secretarias de Ação Social e ao Banco de Alimentos do município de Rio Claro... (Resumo completo, clicar acesso eletrônico abaixo)

Análise higiênico-sanitária de produtos cárneos comercializados na cidade de Botucatu

Brazil is one of the largest producers of animal protein. In 2010, the production of meat (beef, pork and poultry) was estimated at 24.5 million tons and 75% was consumed in the country. The meat is a primary source of water and fat and contains between 20% and 35% protein, providing all essential amino acids and several micronutrients and vitamins. Due to the large consumption of this food, its quality is extremely important and the research of some indicators microorganism become essential in order to ensure the hygienic and sanitary quality, indicating contamination of fecal origin, with the possible presence of pathogens or to identify food spoilage. Thus, the aim of study was to determine the microbiological quality of 90 samples handled meat products and pre- prepared marketed in Botucatu city, according to the parameters required by ANVISA (RDC  12, 2001). Among the samples analyzed, all of them were negative for Staphylococcus coagulase positive and were within the limit allowed by ANVISA for the Clostridium sulfite reducer (up to 3 x 103 CFU/ g). The presence of Salmonella was confirmed in only one sample (1.1%), against to the legal parameters. About thermotolerant coliforms, 54.4% of the samples were outside the acceptable limit by law (up to 5 x 103 MPN/g). Therefore, the presence of these microorganisms indicates inadequate hygiene conditions of foodhandlers and equipment used in the, which are considered inadequate for consumption

Biosusceptometria de corrente alternada para avaliação do trânsito gastrintestinal de alimentos líquidos em ratos

The gastrointestinal tract is the main route of nutrients absorption and drugs delivery. Is important to know the parameters related to the tract, like gastric emptying and gastrointestinal transit, in order to better understand the behavior of different kind of meals or drugs passing through the GIT. Many techniques are used to study these parameters, such as manometry, scintigraphy, phenol red, activated charcoal and carbon-13 reading. However, these methods use radiation, are invasive and require animal sacrifice. As an alternative proposal, the Alternate Current Biosusceptometry (ACB), a magnetic technique, has proved to be effective for these studies with small animals, in a noninvasive way, low cost, radiation free and avoiding the animal death. Associating the ACB to magnetic micro or nanoparticles used as tracers, it is possible to observe the meal behavior inside of the GIT. Focusing meanly on liquid meals digestion, this paper had the objective to evaluate the efficiency of the ACB technique in gastric emptying and gastrointestinal transit evaluation of liquid meals in rats. To perform the experiments, magnetic nanoparticles (ferrite, MgFe2O4) were used on a 1,5 ml solution introduced by gavage on similar weight and age rats. The sensor made by 2 pairs of coils, capable of generating and detecting magnetic fields, creates a field on the interest place and when this field is in contact with the marked meal, it changes, resulting on a variation of the measured voltage. The voltage variation is analyzed and is obtained a particle concentration on the interest region. The results showed that is possible to apply the ACB technique on the GIT evaluation of liquid particles digestion, gastric emptying and meal cecum arrival time curves were obtained and from that, is possible to observe a pattern of gastrointestinal transit. Both mean process time values were acquired, proving the technique capability of ...

Staphylococcus sp na produção primária de leite: aspectos de saúde pública

Staphylococcus is one of the more important causes of the called Foodborne Disease(FD), being that from the 40 species described from genus, the more important is Staphylococcus aureus. During years believed that the S. aureus was the only specie from genus able to produce enterotoxins, responsable for the clinical frame in humans, but latest studies report the isolation of other species both positive coagulase (PC) as negative with enterotoxigenic potential. The symptoms of this intoxication appear after a short period of incubation (2-6 hours) and usually characterized by nausea, vomits, abdominal ache, diarrhea, and rarely is fatal. For the toxin to be formed in food is necessary that bacteria population to be at least 105 UFC/g, being that such toxins characterized by presenting great resistance front of gastrointestinal proteases and of homemade termical treatment. Among the main foods that might carry the microorganism, the milk and its derivatives have highlights. The contamination of the product might happen as from the milk from cows with clinical and/or subclinical mastitis, as the Staphylococcus genus is one of the main agents etiologic from this disease, equipments utensils badly sanitized equipments and utensils and from the manipulators. The control of these factors configures as fundamental condition for the achievement of a safe, quality product, which doesn’t offer risk to the consumers

Simulação de contaminação de sedimentos e águas subterrâneas por gasolina e álcool: um estudo de monitoramento de variações das propriedades físico-químicas

Geophysics studies in areas impacted by petroleum derivatives describe abnormalities of both low and high electrical resistivity (the opposite of electrical conductivity), confirmed as contaminant phase by chemical analysis: this contradiction can be explained by degrading processes that naturally occur and create sub products that can change the environment conductivity. Monitoring the variation of the parameters mentioned serves as a comparative basis to the variation in geoelectrical parameters, which identified the correlation between the same contaminant parameters and the difference between their behavior studied apart, as well as its relations with the biodegradation process. The results are applied to the fuel distribution and storage sectors, leading to the diagnosis and monitoring of possible groundwater contamination scenarios, and the knowledge of the area exposure time to the contaminant, besides the better remediation alternative and impacts control. Among some conclusions, the most significant are the decrease in conductivity over time, so as the increase in Eh value in the gasoline contaminated tank, as well as the decrease in the pH value in the second tank with ethanol, which can be attributed to its degradation. Comparing the variations in both tanks, it is evident that Eh, pH and electrical conductivity do not behave temporally in a similar way, although some correlations between Eh and pH can be related.

Estudo de variações bioquímicas e genotoxicidade induzidas por mistura de contaminantes em tilápia (Oreochromis niloticus), como biomarcadores de contaminação ambiental

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Identificação e avaliação da resistência antimicrobiana de leveduras em vegetais minimamente processados

The consumption of minimally processed vegetables has been growing due to consumer demand for products easy to prepare. As it is extremely manipulated, this product can be contaminated with spoilage organisms such as yeasts, that have the ability to decrease the shelf life of food. In this sense, seeking to concerns about the resistance of microorganisms to sanitation methods for food industries and given the need for more studies of the antimicrobial power of ozone, this study aimed to isolate and identify the yeasts present in minimally processed vegetables, and check their antimicrobial resistance to sodium hypochlorite and ozone. 84 samples of minimally processed vegetables were collected. From them, 47 yeast cultures were isolated and submitted for identification and testing of resistance to sodium hypochlorite sanitizers (50, 100, 200 and 400 ppm) and ozone (0.25 and 0.5 ppm). From the total yeast isolated, 85.1% corresponds to Cryptococcus laurentii, 12.8% to Arxula adeninivorans and 2.1% to Debaryomyces hansenii var. fabryii. All of them were resistant to the sanitizers sodium hypochlorite and ozone at all concentrations tested. Given these observations, some actions must be taken during processing to avoid contamination with deteriorative microbiota.