Complete genome of a dengue virus serotype 4 strain from Amazonas, Brazil

Dengue virus (DENV) infections represent a significant concern for public health worldwide, being considered as the most prevalent arthropod-borne virus regarding the number of reported cases. In this study, we report the complete genome sequencing of a DENV serotype 4 isolate, genotype II, obtained in the city of Manaus, directly from the serum sample, applying Ion Torrent sequencing technology. The use of a massive sequencing technology allowed the detection of two variable sites, one in the coding region for the viral envelope protein and the other in the nonstructural 1 coding region within viral populations.

Complete genome sequence of Deltapapillomavirus 4 (bovine papillomavirus 2) from a bovine papillomavirus lesion in Amazon Region, Brazil

The complete genome sequence of bovine papillomavirus 2 (BPV2) from Brazilian Amazon Region was determined using multiple-primed rolling circle amplification followed by Illumina sequencing. The genome is 7,947 bp long, with 45.9% GC content. It encodes seven early (E1, E2,E4, E5, E6,E7, and E8) and two late (L1 and L2) genes. The complete genome of a BPV2 can help in future studies since this BPV type is highly reported worldwide although the lack of complete genome sequences available.

International trends in health science librarianship: part 4--four Southern European countries.

This is the fourth in a series of articles exploring international trends in health science librarianship in four Southern European countries in the first decade of the 21st century. The invited authors are from Greece, Italy, Spain and Portugal. Future issues will track trends in Latin America and Central Europe.

A non-enteric adenovirus A12 gastroenteritis outbreak in Rio de Janeiro, Brazil

A gastroenteritis outbreak that occurred in 2013 in a low-income community in Rio de Janeiro was investigated for the presence of enteric viruses, including species A rotavirus (RVA), norovirus (NoV), astrovirus (HAstV), bocavirus (HBoV), aichivirus (AiV), and adenovirus (HAdV). Five of nine stool samples (83%) from patients were positive for HAdV, and no other enteric viruses were detected. Polymerase chain reaction products were sequenced and subjected to phylogenetic analysis, which revealed four strains and one strain of non-enteric HAdV-A12 and HAdV-F41, respectively. The HAdV-A12 nucleotide sequences shared 100% nucleotide similarity. Viral load was assessed using a TaqMan real-time PCR assay. Stool samples that were positive for HAdV-A12 had high viral loads (mean 1.9 X 107 DNA copies/g stool). All four patients with HAdV-A12 were < 25 months of age and had symptoms of fever and diarrhoea. Evaluation of enteric virus outbreaks allows the characterisation of novel or unique diarrhoea-associated viruses in regions where RVA vaccination is routinely performed.

1.° Valerii Catulli, Veronensis, epigrammatum variorumque carminum liber. — 2.° Priapeia. — 3.° Galli, Forliviensis, carmen de senectute. — 3.° Probae Falconiae centones Virgiliani. — 4.° Arati phaenomena, graecè, manu Joannis Rosi, Cretensis, anno 1488. scripta.

Puteanus

1.° P. Ovidii Nasonis amorum libri tres. — 2.° Ejusdem liber de remedio amoris. — 3.° Ejusdem carmen de pulice. — 4.° Ejusdem fastorum libri sex.

Puteanus

1.° Explicatio fabularum quae in Ovidii metamorphoseon libris occurrunt : authore anonymo. — 2.° Compendium cosmographiae Ptolemaei : authore anonymo. — 3.° Excerpta è libris ruralium commodorum, quos edidit Petrus de Crescentiis. — 4.° Palladii Rutilii Tauri Aemiliani de re rustica libri tredecim. — 5.° Aethici cosinographia. — 6.° Excerptum è libro Raymundi Lullii de memoria artificiali.

Mentellianus

1.° L. Annaei Senecae tragoediae decem, cum scholiis. — 2.° P. Ovidii Nasonis de arte amandi libri tres, cum scholiis. — 3.° Ejusdem liber de remedio amoris, cum scholiis. — 4.° Moretum, quod carmen Virgilio tribuitur : accedunt scholia. — 5.° Senecae liber de vera amicitia, cum scholiis. — 6.° Virgilii bucolica, cum scholiis. — 7.° Rosae, quod carmen Virgilio tribuitur.

Petri de Carcavi

1.° Papinii Statii sylvarum libri quinque : finis quinti desideratur. — 2.° Ejusdem Achilleïdos libri duo : secundi finis desideratur. — 3.° Auli Persii Flacci satyrae sex, cum scholiis. — 4.° Sallustii in Ciceronem declamatio, cum hujus responso. — 5.° Rogerii epistola ad Joannem Maludanum, regium apud Lemovicas Patronum. — 6.° Ovidii fragmenta.

Mentellianus

1.° Caii Plinii Secundi epistolarum libri decem : primi initium et decimi finis desiderantur. — 2.° Ejusdem panegyricus Trajano dictus. — 3.° Latini Pacati Drepanii panegyricus Theodosio dictus. — 4.° Claudii Mamertini Juliano Imperatori gratiarum actio de Consulatu suo. — 5.° Nazarii panegyricus Constantino, Caesari, dictus. — 6.° Eumenii gratiarum actio Constantino, Augusto, Flaviensium nomine. — 7.° Ejusdem panegyricus Constantino, Augusto, dictus. — 8.° Incerti authoris panegyricus Maximiano et Constantino dictus. — 9.° Eumenii panegyricus Constantio, Caesari, dictus. — 10.° Ejusdem oratio pro restaurandis scholis. — 11.° Claudii Mamertini panegyricus Maximiano, Augusto, dictus. — 12.° Genethliacus Maximiani : authore eodem. — 13.° Incerti authoris panegyricus Constantino, Augusto, dictus : finis desideratur.

Bigotianus

1.° Flores et notabilia, è Senecae libris ab anonymo collecta. — 2.° M. T. Ciceronis paradoxa. — 3.° Sancti Gregorii Nazianzeni apologeticus de fuga sua in Pontum. — 4.° Ejusdem oratio de nativitate Christi. — 5.° Ejusdem oratio de baptismo. — 6.° Sancti Hieronymi liber de viris illustribus : accedit Gennadii de eodem argumento liber, cujus finis desideratur. — Hujusce codicis pars decimo quarto, pars decimo quinto saeculo videtur exarata.

Mazarinaeus