Conditional and inducible transgene expression in endothelial and hematopoietic cells using Cre/loxP and tetracycline-off systems

In the present study, the tetracycline-off and Cre/loxP systems were combined to gain temporal and spatial control of transgene expression. Mice were generated that carried three transgenes: Tie2-tTA, tet-O-Cre and either the ZEG or ZAP reporter. Tie2-tTA directs expression of tetracycline-controlled transactivator (tTA) in endothelial and hematopoietic cells under the control of the Tie2 promoter. Tet-O-Cre produces Cre recombinase from a minimal promoter containing the tet-operator (tetO). ZEG or ZAP contains a strong promoter and a loxP-flanked stop sequence, followed by an enhanced green fluorescence protein (EGFP) or human placental alkaline phosphatase (hPLAP) reporter. In the presence of tetracycline, the tTA transactivator produced by Tie-2-tTA is disabled and Cre is not expressed. In the absence of tetracycline, the tTA binds tet-O-Cre to drive the expression of Cre, which recombines the loxP sites of the ZEG or ZAP transgene and results in reporter gene expression. In the present study, the expression of the ZEG or ZAP reporter genes in embryos and adult animals with and without tetracycline treatment was examined. In the presence of tetracycline, no reporter gene expression was observed. When tetracycline was withdrawn, Cre excision was activated and the reporter genes were detected in endothelial and hematopoietic cells. These results demonstrate that this system may be used to bypass embryonic lethality and access adult phenotypes.

Chrestomathia e paraphrasibus Chaldaicis et Talmude ...

ed. Georgius Laurentius Bauer

Chrestomathia e paraphrasibus Chaldaicis et Talmude : delecta noris brevibus et indice verborum difficiliorum illustrata

ed. Georgius Laurentius Bauer

Lehrbuch zur gründlichen Erlernung der jüdischen Sprache für Beamte, Gerichtsverwandte, Advokaten und ... Kaufleute : mit einem vollständigen hebräisch- und jüdischdeutschen Wörterbuche

[Gottfried Selig]

Sēder harê ʿôlām : bšraibung fun ali di in dr welṭ brihmṭi berig und di ṭugend woś ein jedr berg an zich hoṭ ...

Digitalisat der Ausg. Frankfurṭ an der Oder, [1791/92]

Targûm aškenazzî ʿal ḥamiššā ḥûmšê Tôrā

min-... Mōše Desoi ... di iberzetzung oif der (toire) fon Mendel Zohn

Brivšṭellr oder Patšegen hak-ketāv : le-ḥinnûk neʿārîm meyûssād ʿal-pî darkê hal-lāšôn we-ham-melîṣā ...

ḥûbberû mē'ēt ... Ṣevî Hirš Ben- ... Mē'îr

Ms. Ff. Mus. 1339 - Am 2. Advent : Seyd wacker allezeit, und bethet, daß ihr p. ; C. A. T. B., 2 oboe, 2 violini, 1 viola, violoncello et organo

von Teleman[n]. [Textverf.: Gottfried Simonis]

Ms. Ff. Mus. 1119 - Am XI post Trinit. : Herr! sey mir gnädig, denn mir ist angst pp. ; C. A. T. B., 2 violini, 2 oboe se piace, 1 viola, violoncello et organo

von Telemann. [Textdichter: Gottfried Simonis]

Critik der Urtheilskraft

von Immanuel Kant

Ms. Ff. Mus. 1576 - [Erklingt durch gedoppelt annehmliche Töne]

[Georg Philipp Telemann]

Hippokrates Peri opsios : Hippokratis De visu libellus

in memoriam optimi patris Io. Frider. Iugler ... (Elogium Io. Friderici Iugler) separatim et emendatius ed. notisque et aliorum et suis illustr. Io. Henr. Iugler [Johann Heinrich Jugler]

Biographische und litterarische Nachrichten von ehemaligen Lehrern der hebräischen Litteratur in Tübingen

von Christian Friedrich Schnurrer