Webster County, Township 86 North Range 27 West

Des Moines River Plat Maps.

Webster County, Township 86 North Range 28 West

Des Moines River Plat Maps.

Webster County, Township 87 North Range 26 West

Des Moines River Plat Maps.

Webster County, Township 87 North Range 27 West

Des Moines River Plat Maps.

Webster County, Township 87 North Range 28 West

Des Moines River Plat Maps.

Hamilton County, Township 88 North Range 26 West

Des Moines River Plat Maps.

Webster County, Township 88 North Range 27 West

Des Moines River Plat Maps.

Webster County, Township 88 North Range 28 West

Des Moines River Plat Maps.

Webster County, Township 89 North Range 27 West

Des Moines River Plat Maps.

Webster County, Township 89 North Range 28 West

Des Moines River Plat Maps.

Webster County, Township 90 North Range 28 West

Des Moines River Plat Maps.

Humbolt County, Township 92 North Range 28 West

Des Moines River Plat Maps.

Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains.

Conventional methods are sometimes insufficient to identify human bacterial pathogens, and alternative techniques, often molecular, are required. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identified with a valid score 45.9% of 410 clinical isolates from 207 different difficult-to-identify species having required 16S rRNA gene sequencing. MALDI-TOF MS might represent an alternative to 16S rRNA gene sequencing.

Efficient lentiviral gene transfer into corneal stroma cells using a femtosecond laser.

We investigated a new procedure for gene transfer into the stroma of pig cornea for the delivery of therapeutic factors. A delimited space was created at 110 mum depth with a LDV femtosecond laser in pig corneas, and a HIV1-derived lentiviral vector expressing green fluorescent protein (GFP) (LV-CMV-GFP) was injected into the pocket. Corneas were subsequently dissected and kept in culture as explants. After 5 days, histological analysis of the explants revealed that the corneal pockets had closed and that the gene transfer procedure was efficient over the whole pocket area. Almost all the keratocytes were transduced in this area. Vector diffusion at right angles to the pocket's plane encompasses four (endothelium side) to 10 (epithelium side) layers of keratocytes. After 21 days, the level of transduction was similar to the results obtained after 5 days. The femtosecond laser technique allows a reliable injection and diffusion of lentiviral vectors to efficiently transduce stromal cells in a delimited area. Showing the efficacy of this procedure in vivo could represent an important step toward treatment or prevention of recurrent angiogenesis of the corneal stroma.