Identificação molecular e genotipagem pela técnica de Spoligotyping de Mycobacterium tuberculosis isolado da população indígena e não-indígena no Mato Grosso do Sul (MS)

O presente trabalho baseou-se na análise de amostras de isolados de M. tuberculosis provenientes da população indígena e não indígena do Mato Grosso do Sul. As análises foram feitas basicamente por três técnicas de Biologia Molecular: PCR-IS6110 e PRA (para identificação) e Spoligotyping (para genotipagem), ressaltando que somente as amostras negativas para PCR-IS6110 foram submetidas ao PRA. Os objetivos do estudo eram: confirmar por técnicas moleculares de PCR e de PRA a identificação do complexo M. tuberculosis e de outras micobactérias, após o descongelamento e cultivo, analisar a incidência de isolados de M. tuberculosis da região do Mato Grosso do Sul que não tem o IS6110, avaliar a incidência de outras micobactérias entre a população indígena e não indígena do Mato Grosso do Sul, agrupar em famílias, por similaridade epidemiológica os isolados de M. tuberculosis procedentes da população indígena e não indígena com TB e avaliar a presença efetiva de grupos genéticos predominantes; Pela técnica de PCR-IS6110, analisamos um total de 119 isolados clínicos, sendo que apenas 6 apresentaram negatividade, sendo assim submetidos à técnica do PRA. Feito isso, também apresentaram resultado positivo para CMTB, nos permitindo concluir que essas técnicas podem se tornar ferramentas de grande valia no diagnóstico rápido da TB. Pela técnica de genotipagem do Spoligotyping foram analisadas 97 amostras e foi constatado um maciço predomínio da família LAM, principalmente da subfamília LAM 9, representados por 4 SITs, sendo SIT 42 com 44 isolados e SITs 177, 1337 e 1075 com respectivamente 4, 2 e 1 isolados cada (essa subfamília representou 52,6% do total de isolados). Outras famílias, tais como T, H e U também estiveram presentes no estudo em menores proporções

População e áreas de risco: o caso das inundações na área central de São José do Rio Preto-SP

The problem of flooding in urban areas exist in many cities and their causes are as varied as the areas of occupancy or Valley weatherproofing Fund areas of infiltration in the drainage basin. The anthropic activity come triggering changes and impacts on the environment for a long time, and there is a growing need to come up with solutions and strategies that minimize and reverse the effects caused these risk areas. Thus, the present work will address the case study in São José do Rio Preto-SP that is an exception, since the area that is considered of risk lies in the Centre, unlike most lying on the outskirts. This will also analyze the processes of urban expansion and the situations of social and environmental vulnerability, including their interrelations in urban scale. The physical study of the area will serve as an important basis for the work, but it will focus mainly on the population and the problems which this is being in hazardous areas. We will not join a single methodology, wharever, we will follow our knowledge to adapt what we feel that fits better, every time. The importance of this study is that in addition to identifying the problems which the local population suffers with floods, lists measures that minimize the environmental impacts arising through the fostering of public policies

A influência das universidades públicas de São Carlos-SP na dinâmica do espaço geográfico: a importância da população estudantil para o setor imobiliário

The city of San Carlos is known to have important centers of research aimed at developing high technologies, which are: Federal University of São Carlos (UFSCar), the University of São Paulo (USP). Thus, the portion of the population of students who are not from the city of San Carlos, but it has to be present daily to provide their respective courses in San Carlos, has the need to rent property. The housing market, in turn, uses the application of capital to meet the demands of students and business is taking steps that make the production of space and provide new scope of the place, giving new meaning to the settings and new residents. This work goes towards understanding the students, social workers, boosting growth in this market and the influences of space and real estate agents and their actions materialized in space to meet this demand

Análise de 15 STRS autossômicos na população de Araraquara (São Paulo, Brasil)

Os marcadores genéticos mais utilizados para fins de identificação humana são regiões autossômicas de repetições consecutivas curtas (Short Tandem Repeats - STRs) e para interpretar a análise de DNA, os resultados de um caso necessitam ser comparados com os dados de uma pertinente população. O objetivo do trabalho, portanto, foi caracterizar o perfil genético da população de Araraquara, (São Paulo, Brasil) pela análise de 15 STRs autossômicos (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, VWA, D8S1179, TPOX and FGA) inclusos no kit PowerPlex 16 (Promega) e correlacionar esses dados com a história de formação da população. Não foram observados desvio do equilíbrio de Hardy - Weinberg após correção de Bonferroni. Parâmetros forenses apresentaram valores elevados, sendo os marcadores mais polimórficos o Penta E, D18S51 e FGA. A árvore UPGMA (Unweighted Pair- Group Method with Arithmetic Mean) baseada na medida de distância genética mostrou a população de Araraquara agrupada com as populações da região sudeste do Brasil, próxima do grupo europeu e distante das populações Africana e Ameríndia. Estimativas de mistura revelaram que a contribuição parental para a população de Araraquara foi 76% européia, 18% africana e 6% ameríndia

Controle da população de morcegos hematófagos na região de Andradina, São Paulo

Pós-graduação em Ciência Animal - FMVA

Os efeitos do Programa Minha Casa Minha Vida para a população de baixa renda em Marília-SP: a construção de uma ilusão

Pós-graduação em Ciências Sociais - FFC

Fluxo gênico, estrutura genética espacial intrapopulacional e sistema de reprodução hierárquico entre e dentro de frutos em uma pequena população isolada de Genipa americana L., utilizando marcadores microssatélites

Pós-graduação em Agronomia - FEIS

Desenvolvimento da microbiota oral de bebês: análise longitudinal por pirosequenciamento da microbiota de bebês e suas mães dentro de um Programa de Saúde Bucal para Primeira Infância

Pós-graduação em Biopatologia Bucal - ICT

Clima e vulnerabilidade socioespacial: uma avaliação dos fatores de risco na população urbana do município de São Luís (MA)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Relatos sobre o cotidiano da população pobre e o princípio constitucional da dignidade da pessoa humana

The paper demonstrates the magnitude of the constitutional statement of the dignity of the human person subscribed to the Federal Constitution of 1988 as a fundamental principle. Next, it brings reports on quality of life of residents of Vila Esperança, in the municipality of Cubatão (SP), from an ethnographic approach, descriptive and photographic documentation. We could conclude that the Brazilian legal system is organized hierarchically, so the Principle of Human Dignity cannot be disregard. The vital guarantee of a minimum vital floor is essential for poor people to expand the possibilities for the full exercise of human dignity.

Contaminación microbiana en las líneas de agua de las unidades odontológicas

Introduction and Objective: Microorganisms are responsible for multiple infections and pathologies; this is why it is important to control microbes that can be found in the triple syringe used for irrigation in different dental procedures. The aim of this study was to identify cultivable species of microbes (fungus and bacteria) found in some dental units water lines of a private dental clinic from Medellín, Colombia. Materials and Methods: Random samples were taken from 11 chairs from a total of 89; a sample of water of 500ml was collected from the triple syringe of each selected chair. The study aim to search for the presence of total coliforms, filamentous fungi and leavened Results: the average presence of microorganisms was between 40CFU and more of 200 CFU. Microorganisms such as Aeromona salmonicida, Actinobacilus sp and Pseudomona maltophil were isolated. No total coliforms neither fecal coliforms were found. Conclusions: the high levels of contamination suggest that there is a mature biofilm in somewhere of the dental unit water line, but the absence of total and fecalis coliforms suggest that the water had been treated.

Elaboração de um Jogo Digital Educacional sobre Saúde Bucal Direcionado para a População Infantil

Objective: The aim of this study was to develop an educational oral health digital game for 5-to-7-year-old children. Method: The game, called “Dr. Trata Dente”, was based on the approach to three different oral health-related topics: a) bacterial plaque and the main diseases caused by it; b) oral hygiene methods for bacterial plaque control; c) dental caries and preventive measures for this disease. These topics were discussed in sequential order in three different stages of the game. Dr. Trata Dente is represented by the figure of a little super-hero dentist, who talks to the children about oral health during the game. The game is sub-divided according to its propositions to the children, into an association game, a memory game and a coloring game. After its development, the game was evaluated by three professionals of each of the following areas: Dentistry, Pedagogy and Psychology (n=9), who verified the suitability of the concepts presented in the game as regards oral health, linguistic abilities worked with the children, fulfillment of the intended didactic criteria, and the playful aspect of the game. Results: According to the dentists, the dental concepts presented in the game are adequate. According to the pedagogues, the choice of a super-hero was correct and the given explanations are well elaborated, organized and have accessible language, although long. For the psychologists, the game has a potential positive effect on the children’s learning, but there should be more interaction of the character with the children. After this evaluation, changes were made in the game according to the professionals’ suggestions. Conclusion: It was concluded that in spite of the suggested alterations, the game is suitable for teaching oral health by means of children’s play.

Fundo Nacional do Idoso e as políticas de gestão do envelhecimento da população brasileira

Demographic census from the last decades of the twentieth century began to reveal a tendency concerning the increasing average age of the population. The Brazilian government began introducing ways to manage the effects and consequences of this trend, the most recent being the creation of the "Fundos do Idoso" (Funds for seniors) in the Federal, state and municipal spheres. That law allows transferring federal taxes from common citizens and companies to the funds. This article is specifically written as a critical examination of this governmental initiative towards the problem of an ageing population from the point of view as to how the law has been implemented. With the creation of the funds there will be the enlargement and improvement of services destined for seniors. However, the law mentioned above does not predict an active participation of the seniors in the management of this funds and policies that will come as result.

Efeito do adubo orgânico na diversidade bacteriana de solos

Pós-graduação em Microbiologia Agropecuária - FCAV

Estudo da diversidade microbiana e níveis de endotoxinas em lesões endo-periodontais

Pós-graduação em Odontologia Restauradora - ICT