Efeito do tratamento da H. pylori na expressão de citocinas e microRNAs associados ao processo inflamatório

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da produção de citocinas, expressão gênica e análise funcional de células SHED expostas ao LTA bacteriano

Pós-graduação em Odontologia Restauradora - ICT

Efeito de treinamento de saltos verticais e do destreinamento sobre a expressão gênica de vias energéticas da musculatura esquelética de ratos Wistar induzidos à obesidade

Pós-graduação em Fisioterapia - FCT

Expressão diferencial de mRNA em células de cultivo infectadas ple herpesvírus bovino 5

Pós-graduação em Medicina Veterinária - FMVZ

Organização genômima e análise da expressão do gene hnRNP Q-like (Heterogeneous nuclear ribonucleoprotein A-like) retroinserido no cromossomo B de Astatotilapia latifasciata

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Alterações na expressão do Hormônio Concentrador de Melanina (MCH) na área hipotalâmica lateral do rato ao longo do desenvolvimento pós-natal e envelhecimento

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Expressão imuno-histoquímica de KI-67, COX-2, MMP-9 e P53 nos tumores testiculares caninos

Pós-graduação em Ciência Animal - FMVA

Efeitos preventivos do treinamento físico na expressão das proteínas Cox-2 e Vegf de ratos tratados com dexametasona

Dexamethasone is a synthetic glucocorticoid widely used to treat allergic and inflammatory processes. This drug is used in three main situations, are used to contain acute or chronic inflammatory processes, or like immunosuppressive drug's. In these cases the patient will receive high doses for a chronic period and, therefore, has a much greater chance of adverse side effects, such as hypertension, diabetes and dyslipidemia. Dexamethasone promotes deleterious effects on the arachidonic acid pathway, when administered in high doses, because it is a potent anti-inflammatory drug. We recently demonstrated that dexamethasone significantly reduces the protein expression of vascular endothelial growth factor (VEGF) in both skeletal muscle and heart, but the mechanisms involved remain unclear. Meanwhile, exercise has been shown to be effective against high blood pressure, diabetes and dyslipidemia, promoting, among other factors, the increase in VEGF and angiogenesis. One possible explanation for these effects would be the creation of new vessels mediated by inflammation, or by the stimulation of the formation of products of the metabolism of arachidonic acid (AA), such as prostaglandin E2 (PGE2) and VEGF, by increasing the stimulation of the enzymes cyclooxygenase 1 and 2 (COX-1 and COX-2). Little is known about the preventive effects of training on the action of dexamethasone in the arachidonic acid pathway. Therefore, the aim of this study was to determine whether aerobic exercise training, performed before and concomitant treatment with dexamethasone, was able to prevent the effects of the dexamethasone in the protein expression of COX-2 and VEGF. For this, we used young Wistar rats (n = 40) which were randomly divided into 4 groups: sedentary control (SC), sedentary and treated with dexamethasone (SD), trained control (TC) and trained and treated with dexamethasone (TD). These rats performed aerobic exercise training, 60% of maximum capacity, 5

Efeitos preventivos do exercício físico na expressão da miostatina em ratos tratados com Dexametasona

Muscle atrophy is always associated with Dexamethasone (Dexa) treatment, however the mechanisms are not completely understood. This study investigated the effects of Dexa on myostatin and p70S6K protein expression and if previous exercise training (T) can attenuate these effects. Eighty rats were distributed into 4 groups: sedentary control (SC), sedentary treated with Dexa (SD; 0,5 mg/kg per day, i.p., 10 days), trained control (TC) and trained treated with Dexa (TD) and underwent a training period where they were either submitted to a running protocol (60% of physical capacity, 5 days/week for 8 weeks) or kept sedentary. After T period, animals underwent Dexa treatment concomitant with training. Western Blot was performed to identify myostatin and p70S6k protein expression in the tibialis anterior (TA) and soleus (SOL) muscle. Ten days of Dexa treatment increased fasting glucose (SD=+62%), however previous T attenuated this increase (TD=+20%, p<0.05). Dexa determined significant decrease in body weight in TD (-22%) and SD (-25%), followed by TA weight reduction in SD (-23%) and TD (-20%). Previous training could not avoid these decreases. Myostatin protein expression was not altered by dexa treatment or training in TA muscle but in SOL muscle it was significantly modified after T, regardless of treatment (TC=+%23 and TD=+25) compared with their respective controls. The protein p70S6K was not modified neither by dexa nor training in any of the analyzed muscle or condition. The results of this study allowed us to conclude that previous training attenuates the hyperglycemia induced by Dexa, however it did not prevent the body or muscle weight reductions. Even in the presence of muscle atrophy, the expression of myostatin and p70S6K do not justify the mechanisms of muscle loss induced by Dexa, which suggests that other catabolic or anabolic proteins could be involved in the process of muscle atrophy after 10 days of treatment with Dexa

Avaliação dos parâmetros psicomotores de equilíbrio e coordenaçao motora apendicular em crianças obesas

Obesity is a growing problem all over the world, reaching all age groups and social categories. This is a concern ing fact, because the current life style, more and more sedentary, favors the develop of this disease. Children suffer with this ill more and more prematurely and many of its consequences will be carried during all their lives. With the intention of better understand the relationship between obesity and children's motor development, the present research investigated, through psychomotor evaluations of equilibrium and appendicular motor coordination, based on the Exame Neurológico Evolutivo (ENE), the existence of changes in the performance of this variables in obese children and children with normal weight, in their first year on fundamental education in a public state school. The static equilibrium presented itself lower than normal in obese, compared to the normal weight group. Statistically significant differences were not found in respect to dynamic equilibrium and appendicular motor coordination. With that, we can conclude that the static equilibrium suffered influence of obesity, because the centre of gravity might have been changed due to excessive weight

Perfil cinético do movimento pirouette do ballet clássico em bailarinas experientes e não experientes

A dança é uma das formas de expressão artística mais antiga da história da humanidade. O movimento da dança em foco no presente estudo foi a pirouette do ballet clássico. Tal movimento é caracterizado por um ou mais giros em torno do seu próprio eixo, executado sobre uma perna seja em meia ponta ou ponta completa, enquanto a outra está flexionada, com o pé abaixo do joelho da perna de apoio. O presente estudo consistiu em examinar o perfil cinético do movimento pirouette de bailarinas experientes e não experientes no Ballet Clássico. A hipótese é de que as variáveis relativas à força para produzir o giro influencie na realização do pirouette. As participantes do estudo foram convidadas a realizar o pirouette sobre uma plataforma de força. Os resultados do presente estudo demonstraram que as bailarinas experientes aplicam maior força para iniciar o giro em relação às participantes não experientes. Em conjunto, os resultados do presente estudo sugerem que a força vertical aplicada para iniciar o giro, a qual denominamos impulso inicial, parece ser determinante para a boa performance no movimento pirouette da bailarina. Os resultados do presente estudo oferece uma importante contribuição aos técnicos e professores de dança de forma a valorizar questões de controle motor e postural no processo ensino-aprendizagem do pirouette

Ação extra-nuclear do hormônio triiodotironina (T3) na expressão gênica de HIF-1α e TGFα em linhagem celular de adenocarcinoma mamário

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Cães e gatos: expressão das lesões em intoxicações criminais

Pós-graduação em Medicina Veterinária - FMVZ

Escrita e Liberdade: as relações de causa e consequência na expressão discente

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O exercício físico é capaz de melhorar expressão de AMPK e GLUT4 em músculo esquelético de ratos alcoolistas e/ou tabagistas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)