Absolute abundances of benthic foraminifers and stable isotopes from the Alvin dives 3709-3712

The presence of gas hydrates on the Blake Ridge diapir, northeastern Atlantic Ocean, offers an opportunity to study the impact of methane seepage on the ecology and geochemistry of benthic foraminifera in the late Holocene. Three push cores, covering a time span of ~ 1000 yrs, were retrieved from three distinct microhabitats at the top of the diapir at a water depth of ~ 2150 m: (i) sediments away from seepage (control core), (ii) sediments overlain by clusters of methanotrophic and thiotrophic bivalves, and (iii) chemoautotrophic microbial mats. The foraminiferal assemblages at the two seep sites are marked by a reduction in benthic foraminiferal species diversity, coupled with a near-absence of agglutinated species. However, an opportunistic population rise in CH4- or H2S-tolerant calcareous species (e.g., Globocassidulina subglobosa and Cassidulina laevigata) that utilize the abundant trophic resources at the seeps has led to an increase in the overall assemblage density there. The delta18O and delta13C values of three species of benthic foraminifera - Gyroidinoides laevigatus, Globocassidulina subglobosa, and Uvigerina peregrina - and the planktonic species Globorotalia menardii were acquired from all three cores. The benthic species from methane seeps yield delta13C values of 0.1 to - 4.2 (per mil VPDB), that are distinctly more 13C-depleted relative to the delta13C of 0.4 to - 1.0 (per mil VPDB) at the control (off seep) site. The species from a mussel-bed site exhibit more negative delta13C values than those from microbial mats, possibly reflecting different food sources and higher rate of anaerobic oxidation of methane. The positive delta13C values in the paired planktonic species suggest that authigenic carbonate precipitation did not overprint the observed 13C depletions. Hence the probable cause of negative delta13C of benthic foraminifera is primary calcification from Dissolved Inorganic Carbon (DIC) containing mixed carbon fractions from (a) highly 13C-depleted, microbially-oxidized methane and (b) a seawater source.

Benthic foraminifera in surface sediments of the Persian Gulf

During "Meteor"cruise 1965 the author collected 134 samples of surface sediments from the Iranian part of the Persian Gulf. Benthic Foraminifera populations have been analysed for determining their depth zonation. These data are supposed to allow detailed depth interpretation of Pleistocene sediments found in cores. In addition, the ecological information might be usefull to reconstruct the depositional environment of fossil sediments in similar shallow epicontinental seas. The investigation is published in two parts: the present part 1 contains the catalogue of species with short discussions of taxonomic problems, notes on the distribution within the Persian Gulf and 11 plates, partly with scanning electron micrographs. The results of the statistical analysis are given in data tables which include number of species, percentages of 2 (and 5) ranked species, standing crop and foraminiferal numbers. The author used "species groups" to avoid ambiguities with species requiring additional taxonomic studies. However, species numbers within these units are estimated to yield applicable diversity information. - A total of 52 species and 7 "species groups" were separated, 2 new species were described.

Early Cretaceous planktonic foraminifera from the Tethys

Early Cretaceous planktonic foraminiferal assemblages include rare planispiral and pseudoplanispiral morphotypes with elongate chambers that BouDagher-Fadel et al. assigned to Schackoina or accommodated in the new genus Claviblowiella. New findings of well-preserved planktonic foraminiferal faunas from the Lesches en Diois (SE France) section, the Cismon core (NE Italy), the Calabianca (NW Sicily) section and the Upper Aptian of Deep Sea Drilling Project (DSDP) Site 545 drilled off Morocco, have allowed a better understanding of the morphological features of these rare, unevenly distributed taxa. Our data demonstrate that each small planispiral species with globular chambers has a corresponding "clavate" morphotype which (as the "normal" forms) exhibits a smooth, finely perforate wall. Consequently, the latter have been assigned here to the genus Globigerinelloides and treated as subspecies of the "non-clavate" taxa. The (clavate) subspecies belonging to the genus Globigerinelloides here retained are G. duboisi sigali Longoria, G. maridalensis elongatus subsp. nov., G. blowi lobatus subsp. nov. and G. paragottisi clavatus subsp. nov., while Globigerinelloides minai Obregòn de la Parra is not retained. In addition, a new genus, Pseudoschackoina, type species Planomalina saundersi Bolli (senior synonym of Hastigerinoides cepedai Obregòn de la Parra, has been formalised for individuals possessing elongate, pointed, laterally compressed chambers, bearing tubulospines arranged on a pseudoplanispiral (dysaxial) coiling mode. Stratigraphically, in the sections studied the first taxon to appear is Pseudoschackoina saundersi, in the uppermost part of the Selli Level (=OAE1a), immediately followed, just above the OAE1a, by all the "clavate" globigerinelloidids. Regarding the last occurrences, Pseudoschackoina saundersi and G. maridalensis elongatus disappear in the lower part of the Globigerinelloides algerianus Zone, Globigerinelloides paragottisi clavatus at the top of the same zone, while Globigerinelloides blowi lobatus and G. duboisi sigali range up to the lower part of the Ticinella bejaouaensis Zone.

Rhabdocline weirii; Hemiphacidiaceae

Rhabdocline weirii subsp. oblonga subsp. oblonga A.K.Parker & J.Reid, determined by G. D. Darker

Serum and salivary IgA antibody responses to Saccharomyces cerevisiae, Candida albicans and Streptococcus mutans in orofacial granulomatosis and Crohn's disease

Orofacial granulomatosis (OFG) is a condition of unknown aetiology with histological and, in some cases, clinical association with Crohn's disease (CD). However, the exact relationship between OFG and CD remains uncertain. The aim of this study was to determine whether OFG could be distinguished immunologically from CD by comparing non-specific and specific aspects of humoral immunity in serum, whole saliva and parotid saliva in three groups of patients: (a) OFG only (n = 14), (b) those with both oral and gut CD (OFG + CD) (n = 12) and (c) CD without oral involvement (n = 22) and in healthy controls (n = 29). Non-specific immunoglobulin (IgA, SigA, IgA subclasses and IgG) levels and antibodies to whole cells of Saccharomyces cerevisiae, Candida albicans and Streptococcus mutans were assayed by enzyme-linked immunosorbent assay (ELISA) in serum, whole saliva and parotid saliva. Serum IgA and IgA1 and IgA2 subclasses were raised in all patient groups (P < 0.01). Salivary IgA (and IgG) levels were raised in OFG and OFG + CD (P < 0.01) but not in the CD group. Parotid IgA was also raised in OFG and OFG + CD but not in CD. The findings suggest that serum IgA changes reflect mucosal inflammation anywhere in the GI tract but that salivary IgA changes reflect involvement of the oral cavity. Furthermore, the elevated levels of IgA in parotid saliva suggest involvement of the salivary glands in OFG. Serum IgA antibodies to S. cerevisiae were raised markedly in the two groups with gut disease while serum IgA (or IgG) antibodies to C. albicans were elevated significantly in all three patient groups (P < 0.02). No differences were found with antibodies to S. mutans. Whole saliva IgA antibodies to S. cerevisiae (and C. albicans) were raised in the groups with oral involvement. These findings suggest that raised serum IgA antibodies to S. cerevisiae may reflect gut inflammation while raised SIgA antibodies to S. cerevisiae or raised IgA or IgA2 levels in saliva reflect oral but not gut disease. Analysis of salivary IgA and IgA antibodies to S. cerevisiae as well as serum antibodies in patients presenting with OFG may allow prediction of gut involvement.

Role of Diadegma semiclausum (Hymenoptera: Ichneumonidae) in controlling Plutella xylostella (Lepidoptera: Plutellidae): Cage exclusion experiments and direct observation

We evaluated the role of the larval parasitoid, Diadegma semiclausum Hellen (Hymenoptera: Ichneumonidae), in controlling Plutella xylostella (L.) (Lepidoptera: Plutellidae) by cage exclusion experiments and direct field observation during the winter season in southern Queensland, Australia. The cage exclusion experiment involved uncaged, open cage and closed cage treatments. A higher percentage (54-83%) of P. xylostella larvae on sentinel plants were lost in the uncaged treatment than the closed (4-9%) or open cage treatments (11-29%). Of the larvae that remained in the uncaged treatment, 72-94% were parasitized by D. semiclausum , much higher than that in the open cage treatment (8-37% in first trial, and 38-63% in second trial). Direct observations showed a significant aggregation response of the field D. semiclausum populations to high host density plants in an experimental plot and to high host density plots that were artificially set-up near to the parasitoid source fields. The degree of aggregation varied in response to habitat quality of the parasitoid source field and scales of the manipulated host patches. As a result, density-dependence in the pattern of parasitism may depend on the relative degree of aggregation of the parasitoid population at a particular scale. A high degree of aggregation seems to be necessary to generate density-dependent parasitism by D. semiclausum . Integration of the cage exclusion experiment and direct observation demonstrated the active and dominant role of this parasitoid in controlling P. xylostella in the winter season. A biologically based IPM strategy, which incorporates the use of D. semiclausum with Bt, is suggested for the management of P. xylostella in seasons or regions with a mild temperature.

Evaluation of the impact of natural enemies on Plutella xylostella L. (Lepidoptera: Yponomeutidae) populations on commercial Brassica farms

1 Accurate assessment of the impact of natural enemies on pest populations is fundamental to the design of robust integrated pest management programmes. In most situations, diseases, predators and parasitoids act contemporaneously on insect pest populations and the impact of individual natural enemies, or specific groups of natural enemies, is difficult to interpret. These problems are exacerbated in agro-ecosystems that are frequently disrupted by the application of insecticides. 2 A combination of life-table and natural enemy exclusion techniques was utilized to develop a method for the assessment of the impact of endemic natural enemies on Plutella xylostella populations on commercial Brassica farms. 3 At two of the experimental sites, natural enemies had no impact on P. xylostella survival, at two other sites, natural enemy impact was low but, at a fifth site, natural enemies drastically reduced the P. xylostella population. 4 The calculation of marginal death rates and associated k-values allowed the comparison of mortality factors between experimental sites, and indicated that larval disappearance was consistently the most important mortality factor, followed by egg disappearance, larval parasitism and pupal parasitism. The appropriateness of the methods and assumptions made to calculate the marginal death rates are discussed. 5 The technique represents a robust and easily repeatable method for the analysis of the activity of natural enemies of P. xylostella, which could be adapted for the study of other phytophagous pests.