Freimüthige Prüfung der Schrift des Herrn Professor Rühe, über die Ansprüche der Juden an das deutsche Bürgerrecht

Von M. Hess

Probing interactions of NMD factors in a distance‐dependent manner by BioID

The understanding of molecular mechanisms requires the elucidation of protein-­‐protein interaction in vivo. For large multi-­‐factor complexes like those assembling on mRNA, co-­‐immunoprecipitation assays often identify many peripheral interactors that complicate the interpretation of such results and that might conceal other insightful mechanistic connections. Here we address the protein-­‐protein interaction network for key factors in the nonsense-­‐mediated mRNA decay (NMD) pathway in a distant-­‐dependent manner using BioID1,2. In this novel approach, the mutant E. coli biotin-­‐protein ligase BirAR118G is fused to the bait protein and biotinylates proximal proteins promiscuously. Hence, interactors positioned close to the bait in vivo are enriched by streptavidin purification and identified by mass spectrometry or western blotting. We present a validation of the BioID assay and preliminary results for close interactors of UPF1 and other key players in NMD.

Ein Wort an den Herrn Professor Heinrich von Treitschke

von J. Glück

Professor Sombarts Forschungsergebnisse zur Judenfrage : eine zeitgemäße Betrachtung

von J. Henningsen

Versuch einer Würdigung der Angriffe des Herrn Professor Fries auf die Juden

von Sigmund Zimmern

Cassel! : Predige deinen Juden und dir selbst : ein Mahnwort an Herrn Judenmissionar Professor Dr. Paulus Calles u. dessen Stammesgenossen

von Ludwig Berthold

Offener Brief an den königlichen Geheimen Regierungsrath Dr. Heinrich von Treitschke ordentlicher Professor an der Unviersität Berlin

von e. deutschen Israeliten [d.i. Arnold Kalischer]

Professor Paulsen und die Judenfrage

von Gustav Levinstein

Offener Brief an Herrn Professor Heinrich von Treitschke

von M. Joe͏̈l

Offener Brief an Herrn Professor Franz Delitzsch, Geh. Oberkirchenrath zu Leipzig

von Adolf Blumenthal

Offener Brief eines Juden an Herrn Professor Dr. Virchow

von D. Cassel

"Hochgeehrter, lieber Herr Professor Freud" (Radiogespräch, Sendung «Perspektiven», SRF2)

Sigmund Freud, der Begründer der Psychoanalyse, pflegte zeitlebens viele Freundschaften mit Fachkollegen. Aber kaum eine dauerte so lange wie jene mit dem Schweizer Pfarrer und Psychoanalytiker Oskar Pfister.

Gegen Gildemeister : Herrn Professor Gildemeisters Gutachten über den jüdischen Ritualkodex (Schulchan Aruch) u. das Verhältniss der Juden zu demselben : krit. beleuchtet

M. Joel

Probing interactions of NMD factors in a distance-dependent manner by BioID

The understanding of molecular mechanisms requires the elucidation of protein-protein interaction in vivo. For large multi-factor complexes like those assembling on mRNA, co-immunoprecipitation assays often identify many peripheral interactors that complicate the interpretation of such results and that might conceal other insightful mechanistic connections. Here we address the protein-protein interaction network for key factors in the nonsense-mediated mRNA decay (NMD) pathway in a distant-dependent manner using BioID1,2. In this novel approach, the mutant E. coli biotin-protein ligase BirAR118G is fused to the bait protein and biotinylates proximal proteins promiscuously. Hence, interactors positioned close to the bait in vivo are enriched by streptavidin purification and identified by mass spectrometry or western blotting. We present a validation of the BioID assay and preliminary results for close interactors of UPF1 and other key players in NMD.

Probing interactions of NMD factors in a distance-dependent manner by BioID

The understanding of molecular mechanisms requires the elucidation of protein-protein interaction in vivo. For large multi-factor complexes like those assembling on mRNA, co-immunoprecipitation assays often identify many peripheral interactors that complicate the interpretation of such results and that might conceal other insightful mechanistic connections. Here we address the protein-protein interaction network for key factors in the nonsense-mediated mRNA decay (NMD) pathway in a distant-dependent manner using BioID1,2. In this novel approach, the mutant E. coli biotin-protein ligase BirAR118G is fused to the bait protein and biotinylates proximal proteins promiscuously. Hence, interactors positioned close to the bait in vivo are enriched by streptavidin purification and identified by mass spectrometry or western blotting. We present a validation of the BioID assay and preliminary results for close interactors of UPF1 and other key players in NMD.