Caracterização do gene chi_l555 e propriedades bioquímicas da quitinase de Bacillus thuringiensis

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Hiperacumulação de cádmio em plântulas de Cajanus cajan inoculadas com Pseudomonas

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Eficácia de duas formulações de vacinas autógenas para o controle da lactococose em surubins híbridos Pseudoplatystoma corruscans x Pseudoplatystoma reticulatum

Pós-graduação em Aquicultura - FCAV

Differential gene expression in Acidithiobacillus ferrooxidans LR planktonic and attached cells in the presence of chalcopyrite

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Interleukin-6 polymorphism and Helicobacter pylori infection in Brazilian adult patients with chronic gastritis

Helicobacter pylori is recognised as the most common cause of chronic active gastritis and this bacterium is also an important pathogenic factor in peptic ulcer disease. The biological factors that influence clinical outcome in H. pylori infection have been extensively studied. In addition to immunological factors in the host, bacterial virulence determinants in H. pylori strains are likely to play a crucial role in gastric cancer development. Single-nucleotide polymorphisms at the 5' flanking region of the interleukin (IL)-6 gene promoter (G or C at -174 base) have been identified and individuals with the G allele at position -174 have been shown to produce higher levels of IL-6 than those with the C/C genotype. The mucosal levels of IL-6 were reported to be increased in H. pylori-associated gastritis. The present study was conducted to examine any relationship between inflammatory cytokine polymorphisms and the inflammatory process in mucosa infected by H. pylori. In our study we did not find any association between the C and G alleles in adult patients with chronic gastritis and inflammatory process in gastric mucosa.

Proteins, Pathogens, and Failure at the Composite-Tooth Interface

In the United States, composites accounted for nearly 70% of the 173.2 million composite and amalgam restorations placed in 2006 (Kingman et al., 2012), and it is likely that the use of composite will continue to increase as dentists phase out dental amalgam. This trend is not, however, without consequences. The failure rate of composite restorations is double that of amalgam (Ferracane, 2013). Composite restorations accumulate more biofilm, experience more secondary decay, and require more frequent replacement. In vivo biodegradation of the adhesive bond at the composite-tooth interface is a major contributor to the cascade of events leading to restoration failure. Binding by proteins, particularly gp340, from the salivary pellicle leads to biofilm attachment, which accelerates degradation of the interfacial bond and demineralization of the tooth by recruiting the pioneer bacterium Streptococcus mutans to the surface. Bacterial production of lactic acid lowers the pH of the oral microenvironment, erodes hydroxyapatite in enamel and dentin, and promotes hydrolysis of the adhesive. Secreted esterases further hydrolyze the adhesive polymer, exposing the soft underlying collagenous dentinal matrix and allowing further infiltration by the pathogenic biofilm. Manifold approaches are being pursued to increase the longevity of composite dental restorations based on the major contributing factors responsible for degradation. The key material and biological components and the interactions involved in the destructive processes, including recent advances in understanding the structural and molecular basis of biofilm recruitment, are described in this review. Innovative strategies to mitigate these pathogenic effects and slow deterioration are discussed.

Antimicrobial activity of natural products against Helicobacter pylori: a review

Throughout the genetic and physiological evolution of microorganisms, the microbiological sciences have been expanding the introduction of new therapeutic trials against microbial diseases. Special attention has been paid to the bacterium Helicobacter pylori, which induces gastric infections capable of causing damage, ranging from acute and chronic gastritis to the development of gastric cancer and death. The use of compounds with natural origins has gained popularity in scientific research focused on drug innovation against H. pylori because of their broad flexibility and low toxicity. The aim of this study was to describe the use of natural products against H. pylori in order to clarify important parameters for related fields. The study demonstrated the vast therapeutic possibilities for compounds originating from natural sources and revealed the need for innovations from future investigations to expand the therapeutic arsenal in the fight against H. pylori infection.

Evaluation of the effect of supplementing fermented milk with quinoa flour on probiotic activity

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Atividade antimicrobiana da violaceína em linhagens isoladas de mastite bovina

Violacein is a deeply violet pigment produced by Chromobacterium violaceum, an ubique tropical and subtropical bacterium. This molecule presents many biological activities including antimicrobial, antimycotic, antiviral, antiprotozoal and antitumoral. Important reports pointed to a considerable bactericide activity, which brings an extremely necessary quest. This work evaluated the resistance of some bacterias of interest veterinary. It was obtained significant inhibition activity against Staphylococcus aureus came from bovine mastitis. It was also obtained important synergic relations when violacein and penicillin or cloranphenicol are combined

Tuberculose e sua importância para a pecuária brasileira

Tuberculosis due the large number of victims that come attacking in recent years has become a major concern in the World Health Organization. It is caused by a bacterium that is becoming increasingly resistant to drugs, because it requires long periods in treatment that are not always followed correctly. AIDS has contributed much in recent years with increased morbidity and mortality of Mycobacterium. The Ministry of Agriculture, Livestock and Supply (MAPA) of Brazil in 2001 created the National Program of Control and Eradication of Animal Brucellosis and Tuberculosis (PNCEBT) establishing guidelines such as: sets the method to diagnose, control and certify herds free of disease. Joining the program is voluntary

Endocardite infecciosa em pequenos animais

Infective endocarditis is a process in which an infection attacks the heart endothelial surface, and is commonly caused by bacterial colonization, which is called bacterial endocarditis. It is a condition rarely found in dogs and cats, and is more prevalent in male dogs of large size. It mainly affects the left side of the heart, affecting the mitral and aortic valves with greater frequency. The circulation of the bacterium in the bloodstream is what gives rise endocarditis, and is caused by any non-aseptic process that serves as a gateway for bacterium in the body, as from a skin lesion, even as an invasive procedure, such as, catheterization and surgery. The ante-mortem diagnosis is difficult because the clinical signs of endocarditis are varied and common to other diseases, summing up the signs of infection (fever, lethargy, weight loss), and presence of heart murmur and may show signs of congestive heart failure. Thus, the diagnosis is most often through autopsy. To arrive at a diagnosis should be used, besides the history and physical examination, some laboratory tests, especially blood cultures and echocardiography. Treatment is accomplished through the use of antibiotics for long period of time, it is very important to follow the results of susceptibility after its outcome is revealed. The prognosis for bacterial endocarditis ranges from guarded to poor, and can be assessed mainly by the echocardiography. There are few studies in veterinary about the bacterial endocarditis, and the majority is case reports

Implementação do algoritmo de Newman para identificação de comunidades em placas gráficas

Communities are present on physical, chemical and biological systems and their identification is fundamental for the comprehension of the behavior of these systems. Recently, available data related to complex networks have grown exponentially, demanding more computational power. The Graphical Processing Unit (GPU) is a cost effective alternative suitable for this purpose. We investigate the convenience of this for network science by proposing a GPU based implementation of Newman community detection algorithm. We showed that the processing time of matrix multiplications of GPUs grow slower than CPUs in relation to the matrix size. It was proven, thus, that GPU processing power is a viable solution for community dentification simulation that demand high computational power. Our implementation was tested on an integrated biological network for the bacterium Escherichia coli

Atividade in vivo de parasporina extraída de B. thuringiensis sobre o câncer colorretal murino (CT‐26)

Bacillus thuringiensis (Bt), is an environmental Gram-positive spore-forming bacterium that produces crystalline parasporal protein (Cry) during sporulation. The inclusions often exhibit strong and specific insecticidal activity, making Bt an agent for agricultural controlling insects pest, mites, protozoa and nematodes. Recent studies reported that some of these Crys do not show cytotoxicity against insects but they are capable to kill some human and animal cancer cells. These proteins were denominated parasporins (PS). However, antitumor activity of Bt parasporin on the development of murine colorectal cancer (CT-26), are not well studies and these are no reports on the in vivo effect of these proteins. Thus, the present study evaluated the in vitro and in vivo anti-tumoral activity of Bt parasporin against the murine colorectal cancer line CT-26. Therefore, Balb/c mice were s.c. inoculated with CT-26 cells and weekly treated with parasporin (i.p.) pre-activated by enzymatic digestion with trypsin or proteinase K. Our results have shown, for the first time, that despite the anti-tumor activity in vitro, parasporin crystals couldn’t combat tumor growth in vivo. Instead, this protein was highly toxic, affecting the liver and spleen, with possible effect on other organs, decreasing the survival of treated animals. The results indicate the need for studies to better detoxification or manipulation of parasporin for therapeutic use and new studies for analysis of toxicological effects of repetitive exposure of farmers to this toxin

Caracterização funcional da proteína XAC1201 envolvida na patogenicidade de Xanthomonas citri subsp. citri

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Análise funcional de um consórcio microbiano de solo e prospecção de genes envolvidos na desconstrução da biomassa

Pós-graduação em Microbiologia Agropecuária - FCAV