Molecular method for the characterization of Coxiella burnetii from clinical and environmental samples: variability of genotypes in Spain

[EN] Background. Coxiella burnetii is a highly clonal microorganism which is difficult to culture, requiring BSL3 conditions for its propagation. This leads to a scarce availability of isolates worldwide. On the other hand, published methods of characterization have delineated up to 8 different genomic groups and 36 genotypes. However, all these methodologies, with the exception of one that exhibited limited discriminatory power (3 genotypes), rely on performing between 10 and 20 PCR amplifications or sequencing long fragments of DNA, which make their direct application to clinical samples impracticable and leads to a scarce accessibility of data on the circulation of C. burnetii genotypes. Results: To assess the variability of this organism in Spain, we have developed a novel method that consists of a multiplex (8 targets) PCR and hybridization with specific probes that reproduce the previous classification of this organism into 8 genomic groups, and up to 16 genotypes. It allows for a direct characterization from clinical and environmental samples in a single run, which will help in the study of the different genotypes circulating in wild and domestic cycles as well as from sporadic human cases and outbreaks. The method has been validated with reference isolates. A high variability of C. burnetii has been found in Spain among 90 samples tested, detecting 10 different genotypes, being those adaA negative associated with acute Q fever cases presenting as fever of intermediate duration with liver involvement and with chronic cases. Genotypes infecting humans are also found in sheep, goats, rats, wild boar and ticks, and the only genotype found in cattle has never been found among our clinical samples. Conclusions: This newly developed methodology has permitted to demonstrate that C. burnetii is highly variable in Spain. With the data presented here, cattle seem not to participate in the transmission of C. burnetii to humans in the samples studied, while sheep, goats, wild boar, rats and ticks share genotypes with the human population.

Differences in diversity, structure, and variability between intertidal and subtidal meiofaunal assemblages

[EN] Meiofaunal assemblages from intertidal and shallow subtidal seabeds were studied at two sites (one dominated by volcanic sands and the other by organogenic sands) at Tenerife (Canary Islands, NE Atlantic Ocean) throughout an entire year (May 2000?April 2001). Specifically, we aimed (i) to test for differences in diversity, structure, and stability between intertidal and subtidal meiofaunal assemblages, and (ii) to determine if differences in the meiofaunal assemblage structure may be explained by environmental factors (granulometric composition, availability of organic matter, and carbonate content in sediments). A total of 103,763 meiofaunal individuals were collected, including 203 species from 19 taxonomic groups (Acari, Amphipoda, Cnidaria, Copepoda, Echinodermata, Gastrotricha, Isopoda, Insecta, Kinorrhyncha, Misidacea, Nematoda, Nemertini, Oligochaeta, Ostracoda, Polychaeta, Priapulida, Sipuncula, Tanaidacea, and Turbellaria). Nematodes were the most abundant taxonomic group. Species diversity was higher in the subtidal than in the intertidal zone at both sites, as a result of the larger dominance of a few species in the intertidal zone. The meiofaunal assemblage structure was different between tidal levels at both sites, the intertidal presenting greater temporal variability (multivariate dispersion) in the meiofaunal assemblage structure than the subtidal. Sediment grain size, here quantified by the different granulometric fractions, explained the variability in meiofaunal assemblage structure to a greater extent than the percentage of carbonates, a variable linked to sediment origin. This study revealed differences in diversity, assemblage structure, and variability between intertidal and subtidal meiofauna.

Analysis of Mediterranean ocean variability using five numerical simulations

Programa de oceanografía

Among -and within- genus variability of the UV-absorption capacity in saxicolous mosses

Centro de Investigação em Biodiversidade e Recursos Genéticos (CIBIO--?UP)

Short-term variability of picoplankton across wind-shear region south of Gran Canaria

Máster en Oceanografía

Coupling between upper ocean layer variability and size-fractionated phytoplankton in a non-nutrient-limited environment

[EN] We describe the coupling between upper ocean layer variability and size-fractionated phytoplankton distribution in the non-nutrient-limited Bransfield Strait region (BS) of Antarctica. For this purpose we use hydrographic and size-fractionated chlorophyll a data from a transect that crossed 2 fronts and an eddy, together with data from 3 stations located in a deeply mixed region, the Antarctic Sound (AS). In the BS transect, small phytoplankton (<20 μm equivalent spherical diameter [ESD]) accounted for 80% of total chl a and their distribution appeared to be linked to cross-frontal variability. On the deepening upper mixed layer (UML) sides of both fronts we observed a deep subducting column-like structure of small phytoplankton biomass. On the shoaling UML sides of both fronts, where there were signs of restratification, we observed a local shallow maximum of small phytoplankton biomass. We propose that this observed phytoplankton distribution may be a response to the development of frontal vertical circulation cells. In the deep, turbulent environment of the AS, larger phytoplankton (>20 μm ESD) accounted for 80% of total chl a. The proportion of large phytoplankton increases as the depth of the upper mixed layer (ZUML), and the corresponding rate of vertical mixing, increases. We hypothesize that this change in phytoplankton composition with varying ZUML is related to the competition for light, and results from modification of the light regime caused by vertical mixing.

Short-term variability and trophic interactions within planktonic community in subtropical waters of he Canary Islands (Northeast atlantic)

ES]Los giros subtropicales abarcan grandes áreas del océano donde la productividad del ecosistema se sostiene a través del reciclado de materia y energía. En estas aguas, la interacción entre la disponibilidad de recursos y la presión de los niveles tróficos superiores determina la dinámica de la comunidad planctónica. Sin embargo, en aguas subtropicales, el conocimiento de la variabilidad temporal o el papel de los diferentes componentes de la comunidad dentro de la red trófica es bastante limitado. En esta tesis se evalúa la variabilidad a corto plazo de los diferentes componentes de la comunidad planctónica. El picoplancton dominó la comunidad salvo durante la época productiva, en la que los organismos autótrofos de mayor tamaño desempeñaron un papel destacado. Nuestros resultados muestran como la variabilidad estacional está relacionada con fuerzas “bottom-up”, mientras que los procesos “top-down” dominan a una escala de tiempo más corta. Encontramos que el microzooplancton ejerce un gran impacto sobre la comunidad microbiana, en organismos tanto autótrofos como heterótrofos. Además, observamos un acoplamiento muy estrecho entre estos consumidores y sus presas. Otro mecanismo que regula la estructura planctónica es la depredación de los migradores verticales sobre el zooplancton. Así, la variabilidad del mesozooplancton epipelágico está controlada por un ciclo de depredación vinculado a la iluminación de la luna. En este trabajo realizamos una simulación de esta variabilidad con la que se obtuvieron valores de mortalidad comunitaria de los que derivamos el flujo de carbono activo hacia la zona mesopelágica. Estos valores calculados de transporte activo de carbono son del mismo orden de magnitud que el flujo gravitacional en aguas subtropicales. En el Atlántico noreste la comunidad marina también podría estar influenciada por las tormentas de polvo sahariano que ocurren con gran frecuencia en la zona. En este sentido, se estudió la respuesta de la comunidad planctónica en un período de deposición de polvo atmosférico de gran intensidad, en el año 2010, sin observar una clara respuesta en términos de producción primaria. Por el contrario, la biomasa de diatomeas y mesozooplancton sí se vio aumentada en gran medida tras el paso de una fuerte tormenta de polvo del Sáhara, mientras que los organismos autótrofos de menor tamaño se vieron afectados negativamente. Los resultados de esta tesis suponen una contribución importante para entender la dinámica planctónica tan compleja en los ecosistemas subtropicales, y además, pone de manifiesto la necesidad de llevar a cabo muestreos oceanográficos a escalas de tiempo más cortas.

Spatial variability of wave energy resources around the Canary Islands

[EN]Spatial variability of wave energy resource around the coastal waters of the Canary Archipelago is assessed by using a long-term data set derived by means of hindcasting techniques. Results revea( the existence of large differences in the energetic content available in different zones of the archipelago, mainly during spring and autumn. Areas with a higher wave power leve( are the north edge of Lanzarote, western side of Lanzarote and Fuerteventura, north and northwest in La Palma and El Hierro, as well as the north coast of Tenerife. The available energy potential slightly decreases in the north side of Gran Canaria and La Gomera.

Short-term variability of picoplankton across the wind-shear region south of Gran Canaria

[EN]We have studied the short-term variability -at temporal scale of days and spatial scale of 5 km- of the hydrographic field, organic and inorganic nutrients, chlorophyll and picoplanktonic abundances, across a 40 Km section crossing a frontal system south of Gran Canaria, where anticyclonic eddies in early-stages of formation and convergent fronts have been widely reported in the past. Each cruise consisted in a 3-4 daily-repeated section, and was carried out at the same period of the year (May) during two consecutive years (2011 and 2012). The main goal of our study was to analyze the picoplankton response to short-term variability at scales not considered in regular oceanographic samplings, even in regions with complex hydrographic fields.