946 resultados para Kentucky State Bar Association


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Presented at 14th annual meeting of the Association of Public Data Users, Washington, D.C., Oct. 24, 1989.

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1889/90 contains: Proceedings and papers of the Alabama educational association, June 24th-26th, 1890.

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Appendix "Annual meeting of the Iowa Shorthorn Breeders' Association."

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Report of the 5th annual meeting published with the Transactions of the Wisconsin State Agricultural Society, v. 34.

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Four earlier editions were issued as Library school bulletins: no. 2, covering the years 1887-1896; no. 11, 1887-1901; no. 31, 1887-1911; no. 48, 1887-1921.

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Thesis (Master's)--University of Washington, 2016-06

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Thesis (Master's)--University of Washington, 2016-06

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Thesis (Master's)--University of Washington, 2016-06

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The microlocalization of Ras proteins to different microdomains of the plasma membrane is critical for signaling specificity. Here we examine the complex membrane interactions of H-ras with a combination of FRAP on live cells to measure membrane affinity and electron microscopy of intact plasma membrane sheets to spatially map microdomains. We show that three separable forces operate on H-ras at the plasma membrane. The lipid anchor, comprising a processed CAAX motif and two palmitic acid residues, generates one attractive force that provides a high-affinity interaction with lipid rafts. The adjacent hypervariable linker domain provides a second attractive force but for nonraft plasma membrane microdomains. Operating against the attractive interaction of the lipid anchor for lipid rafts is a repulsive force generated by the N-terminal catalytic domain that increases when H-ras is GTP loaded. These observations lead directly to a novel mechanism that explains how H-ras lateral segregation is regulated by activation state: GTP loading decreases H-ras affinity for lipid rafts and allows the hypervariable linker domain to target to nonraft microdomains, the primary site of H-ras signaling.