Changes in biomass, chemical composition and nutritive value of Spartina alterniflora due to organic pollution in the Itanhaém River Basin (SP, Brazil)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

New features of Cuban endemic Pinguicula filifolia (Lentibulariaceae) and considerations on its habitat and ecology

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Sewage sludge as nitrogen and phosphorus source for cane-plant and first ratoon crops

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

S-metolachlor efficacy on the control of Brachiaria decumbens, Digitaria horizontalis, and Panicum maximum in mechanically green harvested sugarcane

Objetivou-se com este trabalho avaliar o controle em pré-emergência de Brachiaria decumbens, Digitaria horizontalis e Panicum maximum pelo herbicida S-metolachlor aplicado em pré-emergência em área de cana-de-açúcar colhida mecanicamente sem queima prévia das plantas, com e sem palha sobre o solo. O delineamento experimental foi o de blocos ao acaso, com quatro repetições, em esquema de parcela subdividida 7 x 2. Nas parcelas, foram estudados cinco tratamentos de herbicidas (S-metolachlor a 1,44, 1,92 e 2,40 kg ha-1; clomazone a 1,20 kg ha-1; e isoxaflutole a 0,188 kg ha-1) e duas testemunhas sem aplicação. Nas subparcelas, foi avaliada a manutenção ou não da palha de cana na superfície do solo. A eficácia do herbicida S-metolachlor não foi prejudicada pela presença de 14 ou 20 t ha-1 de palha de cana sobre o solo. Com a manutenção da palha, a dosagem de S-metolachlor para o controle adequado das plantas daninhas foi de 1,44kgha-1. No ambiente sem palha, o S-metolachlor controlou B. decumbens, D. horizontalis e P. maximum nas dosagens de 1.92, 1.44 e 1.92kgha-1, respectivamente. Nas duas condições de palha, os herbicidas clomazone e isoxaflutole foram eficazes para as espécies estudadas. O S-metolachlor não causou nenhum sintoma visível de intoxicação à cana-de-açúcar. O clomazone e o isoxaflutole ocasionaram injúrias visuais às plantas de cana. Os herbicidas estudados não afetaram o número de colmos viáveis por m², a altura e o diâmetro de colmos.

Molecular characterization of group A bovine rotavirus in southeastern and central-western Brazil, 2009-2010

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Hematological parameters in two neotropical freshwater teleost, Leporinus macrocephalus (Anostomidae) and Prochilodus lineatus (Prochilodontidae)

Leporinus macrocephalus is a non-migratory omnivore fish which occurs in waters with a relatively high oxygen rate. Prochilodus lineatus is a migratory fish that inhabits deoxygenated hypolimnion and feeds on detritus material. Red blood cell, thrombocytes and white blood cell counts, hematocrit and hemoglobin, serum electrolytes (sodium, potassium, calcium, magnesium and chloride), metabolic products (total serum protein and plasma glucose) and blood cells major axis length for L. macrocephalus and P. lineatus were compared. White blood cell counts for both species indicated a similar activity of the immune functions. The concentration of total protein, sodium, calcium, magnesium and chloride, MCV, as well as red blood cells, monocytes, and thrombocytes size in L. macrocephalus were higher than in P. lineatus. However, P. lineatus had higher red blood cell counts, hematocrit and hemoglobin, which reflects a considerable adaptation to survive in an environment with low levels of oxygen.

Nutrient contents and production of rocket as affected by nitrogen concentrations in the nutritive solution

O empirismo no uso das soluções nutritivas é freqüente. Muitas vezes a mesma solução nutritiva é usada para diferentes espécies baseando-se apenas em semelhanças morfológicas. No entanto esta situação pode proporcionar desequilíbrio nutricional prejudicando tanto a produção quanto a qualidade do produto face o acúmulo de nitrato. Foi conduzido um trabalho em sistema hidropônico - NFT, com o objetivo de avaliar o efeito da concentração de nitrogênio na solução nutritiva na produção, nos teores de nutrientes e de nitrato em folhas de rúcula (Eruca sativa). O trabalho foi conduzido na primavera de 2003. Foram avaliadas quatro concentrações de nitrogênio na solução nutritiva (60,8; 121,6; 182,5; 243,5 mg L-1) e três cultivares de rúcula (Cultivada, Folha Larga e Selvática), sob delineamento de blocos ao acaso, fatorial 4 x 3, com quatro repetições. A cv. Cultivada apresentou maior estatura e fitomassa fresca de folhas, não diferindo da 'Folha Larga' quanto a número de folhas, teor de nitrato nas folhas e fitomassa seca e fresca da raiz. Houve incremento de NO3-, N, Ca e P e diminuição de Mg, K e S com o aumento da concentração de N na solução nutritiva. O cultivo da cv. Cultivada na concentração de 93 mg L-1 é a mais recomendada em função da maior produtividade e baixo teor de nitrato.

Impact of successive sugarcane harvests and trash management practices on soil microbiological properties

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Crop sequences in no-tillage system: effects on soil fertility and soybean, maize and rice yield

Os resíduos vegetais das culturas, ao se decomporem, alteram os atributos químicos do solo e, como consequência, influenciam a produtividade das culturas em sucessão. O objetivo deste trabalho foi avaliar os atributos químicos do solo e a produtividade das culturas de soja, milho e arroz, cultivadas no verão, em sucessão a culturas de inverno em semeadura direta. O experimento foi realizado em Jaboticabal-SP (48 ° 18 ' 58 '' W e 21 ° 15 ' 22 '' S), em um Latossolo Vermelho eutrófico. O delineamento experimental foi em blocos ao acaso, no esquema em faixas, com três repetições. Os tratamentos foram constituídos pela combinação de quatro sequências de culturas de verão (monoculturas de milho e soja e rotações soja/milho e arroz/feijão/algodão) com sete culturas de inverno (milho, girassol, nabo forrageiro, milheto, guandu, sorgo e crotalária). Os cultivos iniciaram-se em 2002. Após o manejo das culturas de inverno e antes da semeadura das culturas de verão do ano agrícola 2006/2007, foram coletadas amostras de solo nas camadas de 0-2,5; 2,5-5,0; 5-10; 10-20; e 20-30 cm. Nas amostras de solo, foram determinados: teores de matéria orgânica, pH, teores de P (resina), K, Ca e Mg trocáveis e acidez potencial (H + Al). As sequências de verão rotação soja/milho e milho em monocultura proporcionaram no solo menores teores de matéria orgânica na camada de 0-10 cm e de P do solo na camada de 0-20 cm. Na sequência de verão arroz/feijão/algodão, maiores teores de K foram proporcionados pelas culturas de inverno crotalária e nabo forrageiro, na camada de 0-10 cm, e milheto, na de 0-2,5 cm. Crotalária, milheto, nabo forrageiro e sorgo, cultivados no inverno, proporcionaram maiores teores de matéria orgânica no solo na camada de 0-30 cm. Maiores teores de P no solo foram proporcionados pela crotalária, na camada de 0-2,5 cm, e pelo nabo forrageiro, na de 0-5 cm. Maiores produtividades de soja, como monocultura de verão, foram obtidas após nabo forrageiro e crotalária e, quando em rotação com milho no verão, após nabo forrageiro, crotalária e milheto. Maiores produtividades de milho foram obtidas após nabo forrageiro, milheto e guandu, e menor produtividade de arroz foi obtida após sorgo.

Erythrocyte ghost cell-alkaline phosphatase: construction and characterization of a vesicular system for use in biomineralization studies

Alkaline phosphatase is required for the mineralization of bone and cartilage. This enzyme is localized in the matrix vesicle, which plays a role key in calcifying cartilage. In this paper we standardize a method to construction a resealed ghost cell-alkaline phosphatase system to mimic matrix vesicles and examine the kinetic behavior of the incorporated enzyme. Polidocanol-solubilized alkaline phosphatase, free of detergent, was incorporated into resealed ghost cells. This process was time-dependent and practically 50% of the enzyme was incorporated into the vesicles in 40 h of incubation, at 25 degreesC. Alkaline phosphatase-ghost cell systems were relatively homogeneous with diameters of about 300 nm and were more stable when stored at -20 degreesC.Alkaline phosphatase was completely released from the resealed ghost cell-system using only phospholipase C. These experiments confirm that the interaction between alkaline phosphatase and the lipid bilayer of resealed ghost cell is exclusively via glycosylphosphatidylinositol (GPI) anchor of the enzyme.An important point shown is that an enzyme bound to resealed ghost cell does not lose the ability to hydrolyze ATP, pyrophosphate and p-nitrophenyl phosphate (PNPP), but the presence of a ghost membrane, as a support of the enzyme, affects its kinetic properties. Moreover, calcium ions stimulate and phosphate ions inhibit the PNPPase activity of alkaline phosphatase present in resealed ghost cells. (C) 2002 Elsevier B.V. B.V. All rights reserved.

Effects of the season of the year, truck type and location on truck on skin bruises and meat quality in pigs

The purpose of this study was to evaluate the effect of the season of the year (summer vs winter), type of truck (A: single decker vs B : double-decker) and pig location on the truck (front, middle, rear) on the incidence of skin bruising and pork quality variation. For this purpose, 2660 gilts of an average weight of 126.7 (+/- 6.6) kg originating from 19 different farms were used. No interaction between season of the year, type of truck and location on truck was observed. A higher number of bruises on the body at unloading and slaughter (P < 0.0001) and a higher number of bruises on the carcass (P < 0.01) were observed in winter. At unloading a higher number of bruises on the body and on the carcass after slaughter was observed in pigs transported on Truck A (P=0.004 and P=0.05). A higher, although not significant, number of bruises was found on the body of pigs transported in the rear compartment of both trucks. Higher paleness value was found in the longissimus and semimembranosus muscles in summer (P=0.0001) than in winter. Cold and heat stress have a negative influence on skin bruises and meat quality, respectively. Poor vehicle design increases the incidence of bruised carcasses without detracting from pork quality regardless of the climate conditions tested and location of the animal it] the truck. (c) 2006 Elsevier B.V. All rights reserved.

Venous tone and cardiac function in the South American rattlesnake Crotalus durissus: mean circulatory filling pressure during adrenergic stimulation in anaesthetised and fully recovered animals

The effects of adrenergic stimulation on mean circulatory filling pressure (MCFP), central venous pressure (P-CV) and stroke volume (Vs), as well as the effects of altered MCFP through changes of blood volume were investigated in rattlesnakes (Crotalus durissus). MCFP is an estimate of the upstream pressure driving blood towards the heart and is determined by blood volume and the activity of the smooth muscle cells in the veins (venous tone). MCFP can be determined as the plateau in P-CV during a total occlusion of blood flow from the heart.Vs decreased significantly when MCFP was lowered by reducing blood volume in anaesthetised snakes, whereas increased MCFP through infusion of blood (up to 3 ml kg(-1)) only led to a small rise in Vs. Thus, it seems that end-diastolic volume is not affected by an elevated MCFP in rattlesnakes. To investigate adrenergic regulation on venous tone, adrenaline as well as phenylephrine and isoproterenol (alpha- and beta-adrenergic agonists, respectively) were infused as bolus injections (2 and 10 mu g kg(-1)). Adrenaline and phenylephrine caused large increases in MCFP and P-CV, whereas isoproterenol decreased both parameters. This was also the case in fully recovered snakes. Therefore, adrenaline affects venous tone through both alpha- and beta-adrenergic receptors, but the alpha-adrenergic receptor dominates at the dosages used in the present study. Injection of the nitric oxide donor SNP caused a significant decrease in P-CV and MCFP. Thus, nitric oxide seems to affect venous tone.

Effects of corn particle size and physical form of the diet on the gastrointestinal structures of broiler chickens

The objective of this experiment was to investigate the effects of different particle sizes, expressed as Geometric Mean Diameter (GMD) of corn (0.336mm, 0.585mm, 0.856mm and 1.12mm) of mash and pelleted broiler chicken diets on the weight of the gizzard, duodenum and jejunum+ileum; on the pH of the gizzard and small intestine and on the characteristics of the duodenal mucous layer (number and height of villi and crypt depth) in 42-day-old broilers. The physical form and the particle size of the diet had no significant effect on gizzard and intestine pH (p > 0.05). A greater gizzard weight was seen in the birds receiving pelleted diet and particle size of 0.336mm (p < 0.008). However, for the particle sizes of 0.856 and 1.12 mm, a greater weight was found in birds that received mash diet (p < 0.039 and p < 0.006, respectively). Also, gizzard weight was greater with increasing corn GMD independent of the physical form of the diet. In the mash diet, the increase in particle size promoted a quadratic response in the weight of duodenum and jejunum + ileum. The pelleted diet promoted a greater number of villi per transverse duodenum cut (p < 0.007) and greater crypt depth (p < 0.05). As the particle size increased, there was a linear increase of villus height and crypt depth in the duodenum, irrespective of the physical form of the diet.

Performance and morphology of intestinal mucosa of broilers fed mannan-oligosaccharides and enzymes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Genetic parameters for buffalo milk yield and milk quality traits using Bayesian inference

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)