Effects of diets, with different levels of n-3 HUFA, designed specifically for gilthead seabream (Sparus auratus L.) broodstock on spawning quality

The effects of dietary lipid levels in the spawning quality has been studied in several cultured fish species. Works like those of Watanabe et al. (1984 a); Mourente et al. (1989); Dhert et al. (1991); Bruce et al. (1993); Navas et al. (1997); Rodriguez et al. (1998); Lavens et al. (1999); Furuita et al. (2002, 2003 b); Mazorra et al. (2003); Fernandez- Palacios (2005) and Aijun et al. (2005) show that lipids and fatty acids are the dietetic components that have more influence in the spawning quality, specially in those species with continuous spawning which display short vitellogenesis periods and are able to incorporate these dietetic components in eggs during the spawning period. Diets for gilthead sea bream (Sparus aurata) broodstock with dietary levels of 2.84% n-3 HUFA, combined with levels of 250 mg/kg vitamine E rasure good spawning quality. Putting so indicative the importance for an effective utilization of essential fatty acids the use of adequate levels of antioxidants.

Desarrollo embrionario y estudio morfométrico de la fase lecitotrófica de larvas de medregal negro (Seriola rivoliana Valenciennes, 1883)

[EN]The present study describes the main embryonic stages and larval development, in culture conditions, of the almaco jack until the fifth day of life. Also a morphometric study of the eggs and larvae from induced spawning was realized. Larval hatching occurred at 36 hours from fertilization. At 60 hours after hatching, 100% of the larvae had their mouths open. At 72 hours all the larvae had a swimming bladder and a digestive tract sufficiently formed to start exogenous feeding.

Captura, aclimatación y maduración en cuatividad de reproductores salvajes de Pseudocaranx dentex en Canarias

[EN]The present work describes the procedures for capture, maintenance and evolution of a Pseudocaranx dentex stock in the Instituto Canario de Ciencias Marinas. Forty fish captured in a commercial off-shore fish farm, were transferred to on land facilities, acclimatation to tanks and inert food was successfully carried out, with 100% survival after 1 year. Initial fish weight (4,15kg) was , followed and sampled to determine individual growth in weight and size. Also, the evolution of its sexual maturity state was evaluated at capture and one year after to determine the maturation of fish for future juvenile production. Fish mature in captivity, but natural spawn was unsuccessful

Eficacia de la inducción hormonal con distintas dosis de GnRHa en corvina (Argyrosomus regius)

[EN]The objective of this experiment was to determine the efficacy of different GnRHa doses for the induction of the spawning in meagre broodstock born in captivity. Five experimental groups were established, and one control group that was not injected. Fish were acclimated in six 10 m3 tanks, and in each tank there was 3? and 4? with an average weight of 8.33±0.97 and 8.47±1.09 kg respectively. Throughout the experimental period (April 14- June 2 of 2009), broodstock were induced on a weekly basis. Each time one female and two males from each of the five experimental groups were induced with an injection of GnRHa at the doses of 10, 20, 30, 40 and 50 ?g.kg-1 respectively. No significant differences were found in any of the controlled parameters: % of injected females with spawning, latency period and % of spawns. Although the highest spawning ratio was obtained with the lower doses, a statistically significant negative correlation between the doses and percentage spawning was found.

Nuevas aportaciones al estudio de la organogénesis en larvas de corvina (Argyrosomus Regius Asso, 1801)

La corvina (Argyrosomus regius) es un teleósteo de la familia Scianidae, que en los últimos años ha despertado un gran interés en acuicultura por su rápido crecimiento y la calidad de su carne. A su vez, se trata de una de las especies denominadas ?nuevas? que no pertenecen a la familia de los espáridos, y permite diversificar la oferta de la piscicultura marina. Las primeras aportaciones al estudio histológico del desarrollo larvario de esta especie fueron realizados por Jiménez et al. (2007). El presente trabajo incrementa la información existente, durante los dos primeros meses de vida de esta especie, lo que permitirá optimizar el cultivo larvario. Para ello se analiza la relación entre el desarrollo de sistema digestivo, la vejiga natatoria y el sistema visual. Los resultado demuestran que la corvina es una especie de rápido desarrollo y muy susceptible a las condiciones de cultivo durante su fase larvaria, condicionando éstas su crecimiento. A su vez el presente estudio ratifica que las características morfológicas y la organogénesis están directamente relacionadas con la longitud de la larva, independientemente de la edad de la misma.

Efecto de la densidad larvaria y secuencial alimentaria en el cultivo larvario de corvina (Argyrosomus regius Asso, 1801) durante el primer mes de vida

Effect of larval density and feeding sequence on meagre (Argyrosomus regius Asso, 1801) larval rearing during the first month of life. In the present work two comparative studies of the effect of larval density and feeding sequence were performed. For such, two initial larval densities, 50 larvae.l-1 and 100 larvae.l-1 were established. In each density three feeding sequences were tested, applying different combinations of rotifers (Brachionus sp.) and Artemia sp. at different larval ages. After 30dah, standard length, dry weight and final survival were determined. Final survival was affected by initial larval density and feeding sequence. Lower density promotes better growth in dry weight and standard length for all the feeding sequences tested. In addition, feeding sequences affects larval growth and survival. Best result in survival (62,81±4,77%) were obtained with high larval density and T2 feeding sequence.

Optimización del protocolo de destete de corvina (Argyrosomus regius, Asso, 1801)

[EN] Argyrosomus regius is a new species of interest in aquaculture. Nevertheless, few are the works published on this species and less even in larvae culture and weaning. In this study 2 experiments were done, in tanks of 15 l, with larvae of 15 and 34 days of life, respectively to which 2 different protocols for their weaning were applied. The provided food consisted of a mixture of Artemia and microdiet to different concentration. In experiment A, the best results of growth were observed in treatment 1 and worse in treatment 3, nevertheless in the survival the opposite happened. In experiment B, the best results they were reached with larvae submitted to low density and with significant difference between treatment 1 and 2, in survival no significant differences were found between the different treatments.

Effect of rearing system intensiveness on biological features, culture performance and larval quality of meagre (Argyrosomus regius Asso, 1801) larvae

[EN]Meagre, Argyrosomus regius A., is a new species for aquaculture in south Atlantic and Mediterranean regions, that can reach a mean fresh weight of 8.02±2.51g. at 95dah. However, hatchery techniques must be improved to optimize culture performance and larval quality. Eggs of meagre were cultured under intensive (75 indv.l-1 in 2m3 tanks) and semi-intensive system (7.5 indv.l-1 in 40m3 tanks) to evaluate the effect of the intensification on biological features, stress resistance and skeletal deformities. At 30dah, despite in semi-intensive system reared larvae a higher total length (19.08± 2.3mm vs 16.00±1.54mm), dry body weight (13.09± 2.43mg vs 6.46±0.52mg), and survival after the activity test (75.0± 13.8% vs 53.3±11.5%) was found, the use of intensive systems were also very suitable and cost-effective for larval rearing of this species

Eficacia de la inducción hormonal, con distintas dosis de GnRHa, en reproductores de corvina (Argyrosomus regius) : efecto sobre la producción y la calidad de las puestas

Máster Oficial en Cultivos Marinos. Trabajo presentado como requisito parcial para la obtención del Título de Máster Oficial en Cultivos Marinos, otorgado por la Universidad de Las Palmas de Gran Canaria (ULPGC), el Instituto Canario de Ciencias Marinas (ICCM), y el Centro Internacional de Altos Estudios Agronómicos Mediterráneos de Zaragoza (CIHEAM)

Dose-dependent effect of a single GnRHa injection on the spawning of meagre ("Argyrosomus regius") broodstock reared in captivity

[EN]The present study aimed to determine the spawning efficacy, egg quality and quantity of captive breed meagre induced with a single gonadotrophin-releasing hormone agonist (GnRHa) injection of 0, 1, 5, 10, 15, 20, 25, 30, 40 or 50 μg kg–1 to determine a recommended optimum dose to induce spawning. The doses 10, 15 and 20 μg kg–1 gave eggs with the highest quality (measured as: percentage of viability, floating, fertilisation and hatch) and quantity (measured as: total number of eggs, number of viable eggs, number of floating eggs, number of hatched larvae and number of larvae that reabsorbed the yolk sac). All egg quantity parameters were described by Gaussian regression analysis with R2 = 0.89 or R2 = 0.88. The Gaussian regression analysis identified that the optimal dose used was 15 μg kg–1. The regression analysis highlighted that this comprehensive study examined doses that ranged from low doses insufficient to stimulate a high spawning response (significantly lower egg quantities, p < 0.05) compared to 15 μg kg–1 through to high doses that stimulated the spawning of significantly lower egg quantities and eggs with significantly lower quality (egg viability). In addition, the latency period (time from hormone application to spawning) decreased with increasing doses to give a regression (R2 = 0.93), which suggests that higher doses accelerated oocyte development that in turn reduced egg quality and quantity. The identification of an optimal dose for the spawning of meagre, which has high aquaculture potential, represents an important advance for the Mediterranean aquaculture industry.

Obtención en cautividad de puestas naturales del pez de limon(Seriola dumerili), en la isla de Gran Canaria (España).

[EN]In the present study, natural spawning of a broodstock group of greater amberjack (Seriola dumerili) is described with regards to: date, number of spawns, and total number of eggs produced per spawn. Fish werecaught in 2011 in the Canary Islands, Gran Canaria (Canary Islands, Spain), and kept in captivity. At the end of May 2014, the broodstock group was formed by two females with a weight 9.81±1.08 kg, size 90.0±2.82cm, with oovocites > 800μ, and five males 9.46±2.04 kg, 86.75±5.43 cm, all fluent with sperm. Between the 1stof June and 18thof October 2014, a total of 25.60 million eggs were obtained in 23 spawns.

Comparación entre la calidad de puestas naturales e inducidas, mediante inyección e implante de GnRHa, del pez de limón (Seriola dumerili).

[EN]For this study, a total of 19 greater amberjack (Seriola dumerili) broodstock were used. Females with oocites bigger than 600μ, and fluent males with a mean weight of 10.72±1.22 kg, fameles, and 10.77±2.33 kg males. The experimental tanks were circular tanks of 40 m3 capacity. One of the tanks were not induced and the other two were with GnRHa, one with injections and the other by implants. Best results, in all parameters studied were with natural spawns, with a 84.37% of fertilization, 92.21% of viable eggs at 24 hours of spawn, 96.60% of hatching, 69.91% of larvae one day after hatching and 10.78% of 5 day life larvae.