Aspectos fundamentais no desenvolvimento de sistemas microemulsionados contendo anfotericina B para uso oftálmico

As occurs with a number of drugs, the bioavailability of amphotericin B (AmB) used to treat fungal infections by the ocular route remains a great challenge to research scientists. In fact, the poor bioavailability of AmB is due mainly to the corneal barrier, which leads to a precorneal loss and consequent decrease in the absorption of this drug into the intraocular tissues. The toxicity associated with this molecule, together with its poor ability to penetrate the intact corneal epithelium, also represents a major drawback to its clinical use. New effective and safe drug vehicles for ocular delivery of AmB are therefore urgently needed. Microemulsions (MEs) seem to be an interesting system, owing to their transparent appearance, thermodynamic stability and favorable viscosity. Knowledge of the process of formation of AmB-containing MEs, as well as a good understanding of the physical chemistry of such systems, would provide reliable information on the best conditions for the use of these systems as eye drops. The goal of this research was thus to make an approach to this subject by reviewing the main studies on the use of MEs as delivery systems for AmB in topical eye treatment.

Systematics of spiny-backed treefrogs (Hylidae: Osteocephalus): An Amazonian puzzle

Spiny-backed tree frogs of the genus Osteocephalus are conspicuous components of the tropical wet forests of the Amazon and the Guiana Shield. Here, we revise the phylogenetic relationships of Osteocephalus and its sister group Tepuihyla, using up to 6134 bp of DNA sequences of nine mitochondrial and one nuclear gene for 338 specimens from eight countries and 218 localities, representing 89% of the 28 currently recognized nominal species. Our phylogenetic analyses reveal (i) the paraphyly of Osteocephalus with respect to Tepuihyla, (ii) the placement of 'Hyla' warreni as sister to Tepuihyla, (iii) the non-monophyly of several currently recognized species within Osteocephalus and (iv) the presence of low (<1%) and overlapping genetic distances among phenotypically well-characterized nominal species (e.g. O. taurinus and O. oophagus) for the 16S gene fragment used in amphibian DNA barcoding. We propose a new taxonomy, securing the monophyly of Osteocephalus and Tepuihyla by rearranging and redefining the content of both genera and also erect a new genus for the sister group of Osteocephalus. The colouration of newly metamorphosed individuals is proposed as a morphological synapomorphy for Osteocephalus. We recognize and define five monophyletic species groups within Osteocephalus, synonymize three species of Osteocephalus (O. germani, O. phasmatus and O. vilmae) and three species of Tepuihyla (T. celsae, T. galani and T. talbergae) and reallocate three species (Hyla helenae to Osteocephalus, O. exophthalmus to Tepuihyla and O. pearsoni to Dryaderces gen. n.). Furthermore, we flag nine putative new species (an increase to 138% of the current diversity). We conclude that species numbers are largely underestimated, with most hidden diversity centred on widespread and polymorphic nominal species. The evolutionary origin of breeding strategies within Osteocephalus is discussed in the light of this new phylogenetic hypothesis, and a novel type of amplexus (gular amplexus) is described. © 2013 The Norwegian Academy of Science and Letters.

As interrogativas de conteúdo na história do Português brasileir o: uma abordagem discursivo-funcional

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Uma ferramenta alternativa para síntese de circuitos lógicos usando a técnica de circuito evolutivo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Biogeografia histórica do gênero Brachygastra Perty, 1833 (Hymenoptera: Vespidae: Polistinae: Epiponini)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Número do tipo ponto flutuante com precisão estendida

Pós-graduação em Engenharia Mecânica - FEIS

Corpo-mídia ou corpo-suporte?: representações do signo corpo em publicidades de perfumes

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Os direitos personalíssimos, e as obrigações, no poder familiar

Pós-graduação em Direito - FCHS

Uma educação para além do bem e do mal: o limiar da experiência educativa de um móbile solto no furacão

Pós-graduação em Educação - IBRC

O infantil e a educação: uma investigação a partir de narrativa de crianças

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Geoestística e sensoriamento remoto na classificação de imagens em áreas cultivadas com citros

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O erro em sala de aula: subsídios para o ensino do eletromagnetismo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A obra de Bento de Jesus Caraça e a educação matemática: entre encanto e resistências

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Arte e ética em The Bell de Iris Murdoch

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A poética ontológica de uma aprendizagem ou o livro dos prazeres

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)