995 resultados para 159-959C


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Growth, mortality and stock parameters of Johnieops sina (Cuvier) based on the data collected from 1989 to 1994 from Sassoon Dock and New Ferry Wharf landing centres of Greater Bombay has been discussed here. The von Bertallanffy's growth parameters for this species were estimated as follows, L∞= 266 mm, K=0.91 per year and W∞=193 g. This species grows to 159 and 223 mm at the end of first and second year of its life. The mortality parameters estimated were Z=6.17, M=2. 03 and F=4.14. The E and U were calculated as 0.62 and 0.63 respectively. Length cohort and Thompson Bell analyses show that there is no decline in the catches at the present level of fishing. But even by doubling the efforts the catches can go up by only 9.4 % indicating that the fishing efforts is not economical and proportional to the increase in yield.

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Collections of phytoplankton were made by Mr. Durairatnamat various stations between latitude 5°S and 25°S and longitude 78° E and 101° E (Fig. 1) from December 1962 to January 1963 during a cruise of the research vessel "Umitaka Maru" belonging to the Tokyo University of Fisheries. This vessel was engaged in work in connection with the International Indian Ocean Expedition (I.I.O.E.). The collections made from these stations (T.G.) were examined at the Fisheries Research Station, Colombo, for the various diatoms present and the findings are reported in this paper.

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我国牡丹资源丰富、药用历史悠久,但长期以来,资源与药效关系不清严重阻碍着丹皮的产业化发展。建立一套综合评价体系,比较不同牡丹资源间的差异,科学评价、准确定位各地不同品种的利用价值,对稳定我国丹皮质量、可持续、集约化发展丹皮产业具有重要的意义。 本实验以我国丰富的药用牡丹资源为材料,从观赏和药用价值两方面首先探讨综合评价牡丹资源的体系。在观赏价值考察中重点比较了花型、花色、花期等性状;药用价值则从根的产量、重金属含量以及七种主要药效成分含量三方面来比较;重金属含量以原子分光光度法检测,药效成分则以高效液相色谱和质谱进行分离鉴定和定性、定量检测。利用建立的综合评价体系对野生种、各地药用品种进行了评价,对丹皮生产相关环节的问题进行了讨论,并初步探讨了药效成分的遗传规律。结果如下: 1、引种的野生牡丹药用价值不比栽培品种高,建议作为育种材料。 2、不同品种间药效成分含量存在显著差异,综合比较‘JSF’、‘CKL’、‘凤丹’适于药用栽培,‘赵粉’、‘朱砂垒’、‘首案红’等品种适宜观赏栽培,‘太平红’、‘JPH’等可花药兼用。 3、不同的土壤、气候环境对丹皮药效成分含量有明显的影响,丹皮中重金属含量主要与土壤环境关系密切。 4、根的粗细程度与药效成分含量关系不大,建议市场药材分级时充分考虑。 5、丹皮的药效成分含量与株龄、采收期、储藏时期关系密切;建议采收、应用根据各地的品种、气候条件及市场情况而定,在保证较高药效成分含量的前提下,尽量缩短生产、流通周期。 6、催花、嫁接对一些牡丹品种的药用价值影响不大,可合理开发利用。 7、药效成分的遗传有一定的规律,86%的子代丹皮酚含量高于父母本或亲本之一;丹皮酚原苷和没食子酰羟基芍药苷含量受父本影响较大,而芍药苷、苯甲酰芍药苷、丹皮酚新苷、没食子酰丹皮酚苷含量受母本影响较大。

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The present paper gives a comparative account of the hydrological conditions and phytoplankton within the Continental Shelf and beyond the Continental Shelf. 54 water samples were taken for hydrological analysis and 18 surface tows for plankton.

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以人浓缩白细胞来源的CD14+单核细胞为前体,建立体外快速培养树突状细胞(dendritic cell,DC)的方法.采用密度梯度离心和MACS磁珠分选系统,收集高纯度的CD14+单核细胞;以rGM-CSF、rIL-4联合分化2天诱导不成熟DC,再将分化后的细胞以rTNF-α、IL-1β、IL-6、PGE2共同活化2天得到成熟DC.流式细胞仪检测结果表明,分化2天的不成熟DC具有吞噬能力,且表型HLA-DR、CD40、CD80表达在80%以上,CD83、CD86基本小表达,成熟后的DC能够激活T细胞增殖,HLA-DR表达增高,CD83、CD86表达占85%.

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下载PDF阅读器甲状旁腺激素(Parathyroid Hormone,PTH)是治疗骨质疏松症的药物之一.将人工合成全长人PTH(hPTH(1-84))的核苷酸序列插入pThioHis A载体中,然后转化大肠杆菌(Escherichia coli.),在IPTG的诱导下,成功实现了rhPTH(1-84)的原核表达.通过发酵条件的优化,初步确定1:40接种.LB+30% M9盐溶液的发酵培养基,37℃培养至OD600nm=0.8时,加入终浓度为0.6 mmol/L的IPTG,诱导8 h的较优发酵程序.

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The complex, fragmented and diverse aspects of a sustainable development perspective are translated into an eight-point framework that defines a problem boundary larger than that traditionally adopted by civil engineers. This leads to practical questions intended to inform engineers who ask 'am I being sustainable?' during project implementation. The value of the questions is tested against a case history of a wastewater treatment project. This demonstrates the relevance of the questions to successive project delivery phases of defining the problem, choosing a solution and implementing that solution through design, construction and operation. The case history highlights that answers to several of the additional questions raised by considering this wider problem space are currently buried within government and clients' policies, regulations and standard practice; these answers may not be accessible to the professional engineer.

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一种高效热调谐共振腔增强型探测器及其制作方法

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精神分裂症是一种复杂的精神疾病,全世界约有1%的人患有这种疾病.以往的研究发现精神分裂症患者的脑容量比正常人小,且一些精神分裂症易感的DNA序列多态性也同时与脑的结构异常有关,这与精神分裂症的神经发育假说是吻合的.最近研究发现人的GULP1基因的两个SNP(rs2004888和rs4522565)与精神分裂症显著相关.为了研究这两个精神分裂症易感的SNP是否也符合神绎发育假说,我们检测了791个正常人的这两个SNP的基因型并测量它们的脑容量,相关性分析发现这两个SNP和脑容量无关,说明GULP1的精神分裂症易感性存在更加复杂的机制.

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患儿男,10岁,因"双侧隐睾"就诊.患儿系足月、顺产、5岁时发现头颅大,经医院诊断为脑积水.几岁时做双侧隐睾手术.患儿身高120 cm.头颅发育不规则,颜面不对称,自小严重的智力低下,说话不清.

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目的:应用眼镜王蛇毒基因疫苗,免疫BALB/c小鼠,制备单克隆抗体(mAb).方法:应用眼镜王蛇Oh-3基因真核表达质粒pIRES-Oh3,佐以脂质体制成基因疫苗,免疫BALB/c小鼠,取其脾细胞与同源Sp2/0骨髓瘤细胞进行融合,经间接ELISA筛选,有限稀释法克隆,制备mAb.结果:获得了2株分泌抗眼镜王蛇毒mAb的杂交瘤细胞株(F5、 F11),细胞培养上清液的抗体效价分别为3.2×10-1、 6.4×10-1,腹水抗体效价分别为1.28×10-4、 2.56×10-4.结论:成功地制备了2株眼镜王蛇毒mAb.

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近年来,分子细胞遗传学研究已基本证实了染色体的串联融合(端粒一着丝粒融合)是麂属动物核型演化的主要重排方式.尽管染色体串联融合的分子机制还不清楚,但通过染色体的非同源重组,着丝粒区域的卫星DNA被认为可能介导了染色体的融合.以前的研究发现在赤麂和小麂染色体的大部分假定的串联融合位点处存在着非随机分布的卫星DNA.然而在麂属的其他物种中,这些卫星DNA的组成以及在基因组中的分布情况尚未被研究.本研究从黑麂和费氏麂基因组中成功地克隆了4种卫星DNA (BMC5、BM700、BM1.1k和FM700),并分析了这些卫星克隆的特征以及在小麂、黑麂、贡山麂和费氏麂染色体上的定位情况.结果表明,卫星Ⅰ和Ⅱ DNA (BMC5,BM700和FM700)的信号除了分布在这些麂属动物染色体的着丝粒区域外,也间隔地分布在这些物种的染色体臂上.其研究结果为黑麂、费氏麂和贡山麂的染色体核型也是从一个2n=70的共同祖先核型通过一系列的串联融合进化而来的假说提供了直接的证据.

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microRNAs(miRNAs)是一类在转录后水平调控基因表达的不编码蛋白质的小RNA(长度20-24个碱基).其中,miR-124a是一个在哺乳动物中枢神经系统高度表达的miRNA,在神经前体细胞向神经元分化的过程中起着举足轻重的作用.由于miRNAs特异性地识别靶基因的3'端调控区(3'UTR)的靶序列,因此,在人类起源过程中基因3'UTR的单核苷酸序列变异有可能导致miRNA调控的改变.通过靶基因预测和3'UTR区在哺乳动物代表物种间的同源序列比较,我们发现miR-124a的靶基冈中有一个基因(PLOD3)3UTR的靶位点中存在人类特异突变位点.利用体外报告基因系统,发现PLOD3基因3'UTR靶位点中所含的一个人类特异的突变导致miR-124a对PLOD3的调控效率降低.研究表明,miRNAs靶基因3'UTR的序列变异具有功能效应,它有可能足人类中枢神经系统在起源和演化中发挥关键作用的重要遗传机制之一.

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首次报道了中国4种蝙蝠的G-带和C-带核型。大长舌果蝠(Eonycteris spelaea)二倍染色体数目(2n)为36,常染色体臂数(FN)为56;马来假吸血蝠(Megaderma spasma)2n=38,FN=70;黑髯墓蝠(Taphozous melanopogon)2n=42,FN=64;皱唇蝠(Chaerephon plicata)2n=48,FN=54。通过C-带显示,除着丝粒异染色质外,在皱唇蝠的许多染色体臂内和马来假吸血蝠染色体的端粒处也有较多的插入异染色质,大长舌果蝠的基因组中既有臂内异染色质也有端粒异染色质。