A judicialização da política no Brasil : estudo de casos de comissões parlamentares de inquérito e fidelidade partidária

O estudo propõe examinar a judicialização da política no Brasil a partir das decisões do Tribunal Superior Eleitoral e do Supremo Tribunal Federal sobre questões políticas que envolvem dois casos: comissões parlamentares de inquérito e fidelidade partidária.partidária que refletem a interferência do Judiciário nas ações políticas adotadas nesses espaços.

Toxische Wirkung von Schadstoffen auf Fischzellen. Bestimmung von DNA-Strangbrüchen mit dem Comet-Assay

The alkaline comet assay is a method of detecting DNA strand breaks and alkali labile sites in individual cells. The method was used to detect DNA strand breaks in isolated blood cells (leukocytes) of carp (Cyprius carpio). DNA damage have been induced by exposure of the cells to sediment extract. Therefore comet assay can be applied as in vitro bioassay for investigations on toxicity of marine sediments.

疏水纳米SiO_2对微管道流动特性的影响

通过微管道流动试验,探讨疏水纳米SiO_2的降压增注机理.在微尺度流动中,由于表面积/体积非常大,液固界面性质对流动有很大影响.在不同管壁界面条件下,用超纯水在内径约为25μm的毛细管内进行流动特性试验.结果表明:相同压力下,经过油基疏水纳米SiO_2试剂处理后,水在微管中的流量有了较明显地提高;疏水纳米SiO_2吸附能够使微管管壁具有超疏水性,从而产生水流滑移效应,达到减阻增流效果.

Erste Ergebnisse zum Verlauf der Laichsaison von Dorschen in der westlichen Ostsee 1997

Investigations concerning the intensity and duration of spawning seasons of cod stocks are parts of the work of the reproductions ecology of commercial fish stocks in the Baltic Sea. The Institute of Baltic Fisheries Rostock has sampled and analysed data for this goal since 1992. The first data analyses for the spawning season 1997, sampled in the areas Kiel Bay, Mecklenburg Bay and Arkona Sea showed that the process of the spawning season is different compared to the years before. The spawning process starts earlier than the other years in Kiel Bay and Mecklenburg Bay. The results show that a high proportion of the spawning stock was part of the spawning process in this area. The Arkona Sea cod are regarded by ICES definition as belonging to the western cod stock. In this ICES sub-division only a low number of spawned cod was observed in March, the main spawning time of the western cod stock. Opposite to the theory of the stock units intensive spawning activities were observed in June as in the years before in the Arkona Sea.

Ursachen für wechselnde Selektionseigenschaften von Schleppnetzsteerten - Bericht über das EU-Forschungsprogramm VARSEL

Die Hoffnung, daß sich Fischbestände mit Hilfe von technischen Maßnahmen, wie der Festsetzung einer Mindeststeertmaschenöffnung, in Größe und Zusammensetzung regulieren lassen, ist in Kreisen der ischereiadministration und -wissenschaft weit verbreitet. Irritierend wirkt aber die Tatsache, daß von zwei oder mehr unabhängigen Untersuchern selten oder nie identische Ausleseeigenschaften bei der gleichen Maschenöffnung festgestellt werden. Bereits bei der Untersuchung eines einzigen Steerts, hinter dem gleichen Schleppnetz an einem Fangplatz eingesetzt, ergeben sich für die einzelnen Hols erhebliche Unterschiede, die nur durch die mehrfache Wiederholung des gleichen Experiments einigermaßen auszugleichen sind. Das bedingt einen erheblichen Aufwand für Selektionsuntersuchungen, bzw. bedingt die Varianz ihrer Ergebnisse. Während einer Forschungsschiffsreise können selten mehr als zwei oder drei verschiedene Konfigurationen geprüft werden. Für die eigentlich notwendige Wiederholung zu anderer Zeit und an anderem Ort fehlen in der Regel die Mittel.

Plasma channeling by multiple short-pulse lasers

Channeling by a train of laser pulses into homogeneous and inhomogeneous plasmas is studied using particle-in-cell simulation. When the pulse duration and the interval between the successive pulses are appropriate, the laser pulse train can channel into the plasma deeper than a single long-pulse laser of similar peak intensity and total energy. The increased penetration distance can be attributed to the repeated actions of the ponderomotive force, the continuous between-pulse channel lengthening by the inertially evacuating ions, and the suppression of laser-driven plasma instabilities by the intermittent laser-energy cut-offs.

Filamentation control in the temperature gradient argon gas

A novel technique of controlling the evolution of the filamentation was experimentally demonstrated in an argon gas-filled tube. The entrance of the filament was heated by a furnace and the other end was cooled with air, which resulted in the temperature gradient distribution along the tube. The experimental results show that multiple filaments are merged into a single filament and then no filament by only increasing the temperature at the entrance of the filament. Also, the filament can appear and disappear after increasing the local temperature and input pulse energy in turn. This technique offers another degree of freedom to control the filamentation and opens a new way for multi-mJ level monocycle pulse generation through filamentation in the noble gas.

Electron self-injection and acceleration driven by a tightly focused intense laser beam in an underdense plasma

A scheme for electron self-injection in the laser wakefield acceleration is proposed. In this scheme, the transverse wave breaking of the wakefield and the tightly focused geometry of the laser beam play important roles. A large number of the background electrons are self-injected into the acceleration phase of the wakefield during the defocusing of the tightly focused laser beam as it propagates through an underdense plasma. Particle-in-cell simulations performed using a 2D3V code have shown generation of a collimated electron bunch with a total number of 1.4 x 109 and energies up to 8 MeV. (C) 2005 American Institute of Physics.

Design of adjustable superresolving filters based on birefringent crystals

A two-mode adjustable superresolving filter based on a birefringent filter is proposed. This kind of filter has superresolution in two modes of adjustment. One is rotation of the binary pupil filter on the optical axis of the system and the other is the tilt of the filter away from the pupil plane on axis parallel or perpendicular to the optical axis of the crystal. The filters act as complex amplitude filters in the former mode, and as pure phase filters in the latter. By analyzing two superresolving parameters, we obtain the optimal design parameters that ensure a large field of view, a large superresolving range, and a high setting accuracy. This kind of filter can provide more flexibility in practical applications. (c) 2006 Optical Society of America.

Electron flow through cytochrome P450

The cytochromes P450 (P450s) are a remarkable class of heme enzymes that catalyze the metabolism of xenobiotics and the biosynthesis of signaling molecules. Controlled electron flow into the thiolate-ligated heme active site allows P450s to activate molecular oxygen and hydroxylate aliphatic C–H bonds via the formation of high-valent metal-oxo intermediates (compounds I and II). Due to the reactive nature and short lifetimes of these intermediates, many of the fundamental steps in catalysis have not been observed directly. The Gray group and others have developed photochemical methods, known as “flash-quench,” for triggering electron transfer (ET) and generating redox intermediates in proteins in the absence of native ET partners. Photo-triggering affords a high degree of temporal precision for the gating of an ET event; the initial ET and subsequent reactions can be monitored on the nanosecond-to-second timescale using transient absorption (TA) spectroscopies. Chapter 1 catalogues critical aspects of P450 structure and mechanism, including the native pathway for formation of compound I, and outlines the development of photochemical processes that can be used to artificially trigger ET in proteins. Chapters 2 and 3 describe the development of these photochemical methods to establish electronic communication between a photosensitizer and the buried P450 heme. Chapter 2 describes the design and characterization of a Ru-P450-BM3 conjugate containing a ruthenium photosensitizer covalently tethered to the P450 surface, and nanosecond-to-second kinetics of the photo-triggered ET event are presented. By analyzing data at multiple wavelengths, we have identified the formation of multiple ET intermediates, including the catalytically relevant compound II; this intermediate is generated by oxidation of a bound water molecule in the ferric resting state enzyme. The work in Chapter 3 probes the role of a tryptophan residue situated between the photosensitizer and heme in the aforementioned Ru-P450 BM3 conjugate. Replacement of this tryptophan with histidine does not perturb the P450 structure, yet it completely eliminates the ET reactivity described in Chapter 2. The presence of an analogous tryptophan in Ru-P450 CYP119 conjugates also is necessary for observing oxidative ET, but the yield of heme oxidation is lower. Chapter 4 offers a basic description of the theoretical underpinnings required to analyze ET. Single-step ET theory is first presented, followed by extensions to multistep ET: electron “hopping.” The generation of “hopping maps” and use of a hopping map program to analyze the rate advantage of hopping over single-step ET is described, beginning with an established rhenium-tryptophan-azurin hopping system. This ET analysis is then applied to the Ru-tryptophan-P450 systems described in Chapter 2; this strongly supports the presence of hopping in Ru-P450 conjugates. Chapter 5 explores the implementation of flash-quench and other phototriggered methods to examine the native reductive ET and gas binding events that activate molecular oxygen. In particular, TA kinetics that demonstrate heme reduction on the microsecond timescale for four Ru-P450 conjugates are presented. In addition, we implement laser flash-photolysis of P450 ferrous–CO to study the rates of CO rebinding in the thermophilic P450 CYP119 at variable temperature. Chapter 6 describes the development and implementation of air-sensitive potentiometric redox titrations to determine the solution reduction potentials of a series of P450 BM3 mutants, which were designed for non-native cyclopropanation of styrene in vivo. An important conclusion from this work is that substitution of the axial cysteine for serine shifts the wild type reduction potential positive by 130 mV, facilitating reduction by biological redox cofactors in the presence of poorly-bound substrates. While this mutation abolishes oxygenation activity, these mutants are capable of catalyzing the cyclopropanation of styrene, even within the confines of an E. coli cell. Four appendices are also provided, including photochemical heme oxidation in ruthenium-modified nitric oxide synthase (Appendix A), general protocols (Appendix B), Chapter-specific notes (Appendix C) and Matlab scripts used for data analysis (Appendix D).