928 resultados para wound fluid and xanthine oxidase
Resumo:
Dating past mass wasting with growth disturbances in trees is widely used in geochronology as the approach may yield dates of past process activity with up to subannual precision. Past work commonly focused on the extraction of increment cores, wedges, or stem cross sections. However, sampling has been shown to be constrained by sampling permissions, and the analysis of tree-ring samples requires considerable temporal efforts. To compensate for these shortcomings, we explore the potential of visual inspection of wound appearance for dating purposes. Based on a data set of 217 wood-penetrating wounds of known age inflicted to European larch (Larix decidua Mill.) by rockfall activity, we develop guidelines for the visual, noninvasive dating of wounds including (i) the counting of bark rings, (ii) a visual assessment of exposed wood and wound bark characteristics (such as the color and weathering status of wounds), and (iii) the relationship between wound age and tree diameter. A characterization of wounds based on photographs, randomly selected from the data set, reveals that young wounds typically can be dated with high precision, whereas dating errors gradually increase with increasing wound age. While visual dating does not reach the precision of dendrochronological dating, we clearly demonstrate that spatial patterns of and differences in rockfall activity can be reconstructed with both approaches. The introduction of visual dating approaches will facilitate fieldwork, especially in applied research, assist the conventional interpretation of tree-ring signals, and allow the reconstruction of geomorphic processes with considerably fewer temporal and financial efforts.
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Rodents are important reservoirs for a large number of zoonotic pathogens. We examined the occurrence of 11 viral, bacterial, and parasitic agents in rodent populations in Austria, including three different hantaviruses, lymphocytic choriomeningitis virus, orthopox virus, Leptospira spp., Borrelia spp., Rickettsia spp., Bartonella spp., Coxiella burnetii, and Toxoplasma gondii. In 2008, 110 rodents of four species (40 Clethrionomys glareolus, 29 Apodemus flavicollis, 26 Apodemus sylvaticus, and 15 Microtus arvalis) were trapped at two rural sites in Lower Austria. Chest cavity fluid and samples of lung, spleen, kidney, liver, brain, and ear pinna skin were collected. We screened selected tissue samples for hantaviruses, lymphocytic choriomeningitis virus, orthopox viruses, Leptospira, Borrelia, Rickettsia, Bartonella spp., C. burnetii, and T. gondii by RT-PCR/PCR and detected nucleic acids of Tula hantavirus, Leptospira spp., Borrelia afzelii, Rickettsia spp., and different Bartonella species. Serological investigations were performed for hantaviruses, lymphocytic choriomeningitis virus, orthopox viruses, and Rickettsia spp. Here, Dobrava-Belgrade hantavirus-, Tula hantavirus-, lymphocytic choriomeningitis virus-, orthopox virus-, and rickettsia-specific antibodies were demonstrated. Puumala hantavirus, C. burnetii, and T. gondii were neither detected by RT-PCR/PCR nor by serological methods. In addition, multiple infections with up to three pathogens were shown in nine animals of three rodent species from different trapping sites. In conclusion, these results show that rodents in Austria may host multiple zoonotic pathogens. Our observation raises important questions regarding the interactions of different pathogens in the host, the countermeasures of the host's immune system, the impact of the host–pathogen interaction on the fitness of the host, and the spread of infectious agents among wild rodents and from those to other animals or humans.
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Defining new therapeutic strategies to overcome therapy resistance due to tumor heterogeneity in colon cancer is challenging. One option is to explore the molecular profile of aggressive disseminating tumor cells. The cytoskeleton-associated Death-associated protein kinase (DAPK) is involved in the cross talk between tumor and immune cells at the invasion front of colorectal cancer. Here dedifferentiated tumor cells histologically defined as tumor budding are associated with a high risk of metastasis and poor prognosis. Analyzing samples from 144 colorectal cancer patients we investigated immunhistochemical DAPK expression in different tumor regions such as center, invasion front, and buds. Functional consequences for tumor aggressiveness were studied in a panel of colon tumor cell lines using different migration, wound healing, and invasion assays. DAPK levels were experimentally modified by siRNA transfection and overexpression as well as inhibitor treatments. We found that DAPK expression was reduced towards the invasion front and was nearly absent in tumor buds. Applying the ECIS system with HCT116 and HCT116 stable lentiviral DAPK knock down cells (HCTshDAPK) we identified an important role for DAPK in decreasing the migratory capacity whereas proliferation was not affected. Furthermore, the migration pattern differed with HCTshDAPK cells showing a cluster-like migration of tumor cell groups. DAPK inhibitor treatment revealed that the migration rate was independent of DAPK's catalytic activity. Modulation of DAPK expression level in SW480 and DLD1 colorectal cancer cells significantly influenced wound closure rate. DAPK seems to be a major player that influences the migratory capability of disseminating tumor cells and possibly affects the dynamic interface between pro- and anti-survival factors at the invasion front of colorectal cancer. This interesting and new finding requires further evaluation.
Resumo:
Osteal macrophages (OsteoMacs) are a special subtype of macrophage residing in bony tissues. Interesting findings from basic research have pointed to their vast and substantial roles in bone biology by demonstrating their key function in bone formation and remodeling. Despite these essential findings, much less information is available concerning their response to a variety of biomaterials used for bone regeneration with the majority of investigation primarily focused on their role during the foreign body reaction. With respect to biomaterials, it is well known that cells derived from the monocyte/macrophage lineage are one of the first cell types in contact with implanted biomaterials. Here they demonstrate extremely plastic phenotypes with the ability to differentiate towards classical M1 or M2 macrophages, or subsequently fuse into osteoclasts or multinucleated giant cells (MNGCs). These MNGCs have previously been characterized as foreign body giant cells and associated with biomaterial rejection, however more recently their phenotypes have been implicated with wound healing and tissue regeneration by studies demonstrating their expression of key M2 markers around biomaterials. With such contrasting hypotheses, it becomes essential to better understand their roles to improve the development of osteo-compatible and osteo-promotive biomaterials. This review article expresses the necessity to further study OsteoMacs and MNGCs to understand their function in bone biomaterial tissue integration including dental/orthopedic implants and bone grafting materials.
Resumo:
Cellular migration is an integral component of many biological processes including immune function, wound healing and cancer cell metastasis. A complete model illustrating the mechanism by which cells accomplish movement is still lacking. Exploring the affects of various drugs on cell motility may be instrumental in discovering new proteins which mediate cell movement. This project aims ultimately to characterize the molecular target of the drug Cucurbitacin-I, a natural plant product. This drug has been shown to inhibit migration of epithelial sheets and may have anti-tumor activity. In this paper, we show that Cucurbitacin-I inhibits the migration of MDCK and B16F1 cells. The drug also affects the integrity of the actin cytoskeleton of these cells by indirectly stabilizing filamentous actin. Cucurbitacin-I does not, however, have an effect on the motility or cytoskeletal morphology of the soil amoeba, Dictyostelium discoidium.
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Purpose. To evaluate the presence of Community Associated–Methicillin Resistant Staphylococcus Aureus, CA-MRSA, in abscesses and skin and soft tissue infections presenting at 9 urgent care clinics in San Antonio, TX. ^ Methods. During the 40-month retrospective study (April 2006 to August 2009), wound cultures collected in 9 urgent care centers were evaluated for MRSA growth, antibiotics prescribed, follow up wound care, and antibiotic prescribing habits by physicians for all patients presenting with abscesses and skin/soft tissue infections. ^ Results. Across 9 urgent care centers in San Antonio, TX, 36,797 abscesses and cases of skin and soft tissue infections were treated during 40 months. Of the 36,797 cases, 9290 patients had wound cultures sent with 5,630 cultures sent to Texas MedClinic’s primary lab. Of the 5630 cultures sent to their primary lab, this reflected a prevalence of 4727 (84 %) cultures were positive for MRSA. Of the 9290 patients who had a wound culture sent (April 10th, 2006 to August 31st, 2009), a total of 4,307 antibiotics were prescribed. The top five antibiotics prescribed for CA-MRSA were Bactrim (55.5%), Clindamycin (18.4%), Bactroban (5%), Amoxicillin (3.5%), and Doxycycline (3%) representing 85.4% of the antibiotics prescribed. 8809/9290 (94.8%) of patients required no more than 3 follow up visits. Of the 33 physicians working full time during the entire study period, 29/33 (87.8%) of the physicians were family medicine physicians and represented varied prescribing rates of antibiotics between 11-76% with 26/33 (78.8%) of physicians prescribing antibiotics greater than 40% of the time.^ Conclusions. Abscesses and soft tissue infections are a common presenting complaint to urgent care centers. This study reveals that antibiotic-prescribing practices can be improved with physician education since this high prevalence was not known previously. Also, treating abscesses with limited packing has been shown to be a viable option in this particular circumstance and would be open field for additional clinical research. Due to the high prevalence of CA-MRSA skin and soft tissue infections among patients presenting to urgent care centers presumptive treatment for MRSA is indicated. Increasing levels of resistance to penicillin antibiotics is concerning and warrants alternative antibiotic management strategies.^
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Dr. Silas F. Starley deplores what he considered errors generally taught and accepted in the late 19th century in Two Obstetrical Heresies . “The first is the part that membranes containing the amniotic fluid and the foetus play in effecting dilation of the os uteri in the first stage of labor.The second is the supposed necessity for waiting for their rupture and the escape of the waters before applying the forceps, in every case, without exception.” Silas F. Starley (1823-1887) was born in Alabama and moved to Texas with his family in 1837. He graduated from the University of Louisville School of Medicine in 1854 and spent his professional career in Texas, ending his career in Corsicana. He was President of the State Medical Association of Texas (Texas Medical Association) in 1883 and wrote articles in Texas medical journals on various topics including obstetrics, vascular tumor, and pneumonia. Texas State Historical Association, http://www.tshaonline.org/handbook/online/articles/sat05 , accessed 10/16/2012. Texas Physicians Historical Biographical Database, http://www4.utsouthwestern.edu/library/doctors/doctors.cfm?DoctorID=16809 , accessed 10/16/12.
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Background: Surgical site infections (SSIs) after abdominal surgeries account for approximately 26% of all reported SSIs. The Center for Disease Control and Prevention (CDC) defines 3 types of SSIs: superficial incisional, deep incisional, and organ/space. Preventing SSIs has become a national focus. This dissertation assesses several associations with the individual types of SSI in patients that have undergone colon surgery. ^ Methods: Data for this dissertation was obtained from the American College of Surgeons' National Surgical Quality Improvement Program (NSQIP); major colon surgeries were identified in the database that occurred between the time period of 2007 and 2009. NSQIP data includes more than 50 preoperative and 30 intraoperative factors; 40 collected postoperative occurrences are based on a follow-up period of 30 days from surgery. Initially, four individual logistic regressions were modeled to compare the associations between risk factors and each of the SSI groups: superficial, deep, organ/space and a composite of any single SSI. A second analysis used polytomous regression to assess simultaneously the associations between risk factors and the different types of SSIs, as well as, formally test the different effect estimates of 13 common risk factors for SSIs. The final analysis explored the association between venous thromboembolism (VTEs) and the different types of SSIs and risk factors. ^ Results: A total of 59,365 colon surgeries were included in the study. Overall, 13% of colon cases developed a single type of SSI; 8% of these were superficial SSIs, 1.4% was deep SSIs, and 3.8% were organ/space SSIs. The first article identifies the unique set of risk factors associated with each of the 4 SSI models. Distinct risk factors for superficial SSIs included factors, such as alcohol, chronic obstructive pulmonary disease, dyspnea and diabetes. Organ/space SSIs were uniquely associated with disseminated cancer, preoperative dialysis, preoperative radiation treatment, bleeding disorder and prior surgery. Risk factors that were significant in all models had different effect estimates. The second article assesses 13 common SSI risk factors simultaneously across the 3 different types of SSIs using polytomous regression. Then each risk factor was formally tested for the effect heterogeneity exhibited. If the test was significant the final model would allow for the effect estimations for that risk factor to vary across each type of SSI; if the test was not significant, the effect estimate would remain constant across the types of SSIs using the aggregate SSI value. The third article explored the relationship of venous thromboembolism (VTE) and the individual types of SSIs and risk factors. The overall incidence of VTEs after the 59,365 colon cases was 2.4%. All 3 types of SSIs and several risk factors were independently associated with the development of VTEs. ^ Conclusions: Risk factors associated with each type of SSI were different in patients that have undergone colon surgery. Each model had a unique cluster of risk factors. Several risk factors, including increased BMI, duration of surgery, wound class, and laparoscopic approach, were significant across all 4 models but no statistical inferences can be made about their different effect estimates. These results suggest that aggregating SSIs may misattribute and hide true associations with risk factors. Using polytomous regression to assess multiple risk factors with the multiple types of SSI, this study was able to identify several risk factors that had significant effect heterogeneity across the 3 types of SSI challenging the use of aggregate SSI outcomes. The third article recognizes the strong association between VTEs and the 3 types of SSIs. Clinicians understand the difference between superficial, deep and organ/space SSIs. Our results indicate that they should be considered individually in future studies.^
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Microorganisms play an important role in the transformation of material within the earth's crust. The storage of CO2 could affect the composition of inorganic and organic components in the reservoir, consequently influencing microbial activities. To study the microbial induced processes together with geochemical, petrophysical and mineralogical changes, occurring during CO2 storage, long-term laboratory experiments under simulated reservoir P-T conditions were carried out. Clean inner core sections, obtained from the reservoir region at the CO2 storage site in Ketzin (Germany) from a depth of about 650 m, were incubated in high pressure vessels together with sterile synthetic formation brine under in situ P-T conditions of 5.5 MPa and 40°C. A 16S rDNA based fingerprinting method was used to identify the dominant species in DNA extracts of pristine sandstone samples. Members of the alpha- and beta-subdivisions of Proteobacteria and the Actinobacteria were identified. So far sequences belonging to facultative anaerobic, chemoheterotrophic bacteria (Burkholderia fungorum, Agrobacterium tumefaciens) gaining their energy from the oxidation of organic molecules and a genus also capable of chemolithoautotrophic growth (Hydrogenophaga) was identified. During CO2 incubation minor changes in the microbial community composition were observed. The majority of microbes were able to adapt to the changed conditions. During CO2 exposure increased concentrations of Ca**2+, K**+, Mg**2+ and SO4**2- were observed. Partially, concentration rises are (i) due to equilibration between rock pore water and synthetic brine, and (ii) between rock and brine, and are thus independent on CO2 exposure. However, observed concentrations of Ca**2+, K**+, Mg**2+ are even higher than in the original reservoir fluid and therefore indicate mineral dissolution due to CO2 exposure.
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Mud volcanoes (MV) are sources of mass and energy, transported from deeper levels of the sediment pile to the surface. Together with fluid and gas, thermal energy is emitted through these structures. Therefore heat flow determination is a sensible tool to detect and quantify the amount of convective flow. In the Gulf of Cadiz several mud volcanoes can be found along major tectonic lines (SWIM faults). We employ geothermal measurements to observe the activity of mud volcanoes and possible leakage at the faults apart from pronounced structures.
Resumo:
Esta investigación, orientada bajo un enfoque cualitativo-interpretativo, describe las concepciones de un grupo de setenta y ocho profesores de inglés de los niveles de Educación Media General y Educación Universitaria de la ciudad de San Cristóbal, Venezuela, sobre la expansión, uso y enseñanza del inglés como idioma internacional. Desde una perspectiva crítica y posestructuralista en la que se concibe al inglés como un fenómeno social cargado de ideologías y valores culturales, este estudio pretende dar cuenta de las conexiones existentes entre el inglés y las fuerzas económicas, políticas, sociales e ideológicas que subyacen detrás de su expansión, uso y enseñanza en el mundo (Holliday, 2005, 2006, 2013; Pennycook, 1994; Phillipson, 1992, 2010), y la forma en como estas conexiones son percibidas por los profesores de inglés de un contexto específico de la periferia (Canagarajah, 1999a). Para la exploración en profundidad del fenómeno se adopta en este estudio el método fenomenológico (Colás, 1998b) como propuesta metodológica para descubrir la forma en que los profesores de inglés de este contexto específico comprenden el fenómeno y conciben la expansión, uso y enseñanza del inglés como idioma internacional. Como instrumentos para la recolección de los datos se utilizan dos cuestionarios con preguntas abiertas y cerradas administrados por internet a los participantes, y entrevistas semi-estructuradas cara a cara en profundidad con nueve profesores seleccionados como informantes clave. Para el análisis de los datos se toma el proceso analítico de Strauss y Corbin (2002), proceso que apunta hacia la generación inductiva de teoría a través de la comparación constante de los datos para formar categorías. En este estudio, el análisis de los datos comprende un proceso integral y cíclico que toma en cuenta los datos provenientes de todos los participantes y de todos los instrumentos de recolección. También se hace uso técnico del software Atlas.ti versión 6.0 para el almacenamiento y xivmanipulación sistemática de las entrevistas semi-estructuradas en profundidad. Entre los hallazgos se devela una complejidad enmarcada en el estudio de las concepciones de los profesores de este contexto sociocultural específico que resultan ser fluidas y en oportunidades simultáneamente contradictorias entre sí sobre la expansión, uso y enseñanza del inglés como idioma internacional. En tal sentido, los resultados reflejan poca conciencia crítica de los participantes sobre la hegemonía del inglés en el mundo y su percepción del idioma como un asunto necesario y beneficioso para la comunicación internacional, de allí que consideren su enseñanza y aprendizaje como necesarios en todos los contextos del mundo. Este estudio también expresa una concientización de los participantes en algunos aspectos específicos como su sentido de propiedad del idioma y su percepción como hablantes y docentes competentes del inglés en un contexto específico. Asimismo, revela algunos asuntos sociopolíticos enmarcados en la dicotomía 'nativo' - 'no nativo' del inglés fundamentado en el concepto del 'native speakerism'. Al mismo tiempo, muestra la concepción de los participantes sobre la enseñanza del inglés como una actividad apolítica y neutral aunque simultáneamente refleja su inclinación hacia la promoción del pensamiento crítico y reflexivo de los estudiantes a través de sus clases de inglés
Resumo:
Esta investigación, orientada bajo un enfoque cualitativo-interpretativo, describe las concepciones de un grupo de setenta y ocho profesores de inglés de los niveles de Educación Media General y Educación Universitaria de la ciudad de San Cristóbal, Venezuela, sobre la expansión, uso y enseñanza del inglés como idioma internacional. Desde una perspectiva crítica y posestructuralista en la que se concibe al inglés como un fenómeno social cargado de ideologías y valores culturales, este estudio pretende dar cuenta de las conexiones existentes entre el inglés y las fuerzas económicas, políticas, sociales e ideológicas que subyacen detrás de su expansión, uso y enseñanza en el mundo (Holliday, 2005, 2006, 2013; Pennycook, 1994; Phillipson, 1992, 2010), y la forma en como estas conexiones son percibidas por los profesores de inglés de un contexto específico de la periferia (Canagarajah, 1999a). Para la exploración en profundidad del fenómeno se adopta en este estudio el método fenomenológico (Colás, 1998b) como propuesta metodológica para descubrir la forma en que los profesores de inglés de este contexto específico comprenden el fenómeno y conciben la expansión, uso y enseñanza del inglés como idioma internacional. Como instrumentos para la recolección de los datos se utilizan dos cuestionarios con preguntas abiertas y cerradas administrados por internet a los participantes, y entrevistas semi-estructuradas cara a cara en profundidad con nueve profesores seleccionados como informantes clave. Para el análisis de los datos se toma el proceso analítico de Strauss y Corbin (2002), proceso que apunta hacia la generación inductiva de teoría a través de la comparación constante de los datos para formar categorías. En este estudio, el análisis de los datos comprende un proceso integral y cíclico que toma en cuenta los datos provenientes de todos los participantes y de todos los instrumentos de recolección. También se hace uso técnico del software Atlas.ti versión 6.0 para el almacenamiento y xivmanipulación sistemática de las entrevistas semi-estructuradas en profundidad. Entre los hallazgos se devela una complejidad enmarcada en el estudio de las concepciones de los profesores de este contexto sociocultural específico que resultan ser fluidas y en oportunidades simultáneamente contradictorias entre sí sobre la expansión, uso y enseñanza del inglés como idioma internacional. En tal sentido, los resultados reflejan poca conciencia crítica de los participantes sobre la hegemonía del inglés en el mundo y su percepción del idioma como un asunto necesario y beneficioso para la comunicación internacional, de allí que consideren su enseñanza y aprendizaje como necesarios en todos los contextos del mundo. Este estudio también expresa una concientización de los participantes en algunos aspectos específicos como su sentido de propiedad del idioma y su percepción como hablantes y docentes competentes del inglés en un contexto específico. Asimismo, revela algunos asuntos sociopolíticos enmarcados en la dicotomía 'nativo' - 'no nativo' del inglés fundamentado en el concepto del 'native speakerism'. Al mismo tiempo, muestra la concepción de los participantes sobre la enseñanza del inglés como una actividad apolítica y neutral aunque simultáneamente refleja su inclinación hacia la promoción del pensamiento crítico y reflexivo de los estudiantes a través de sus clases de inglés
Resumo:
Esta investigación, orientada bajo un enfoque cualitativo-interpretativo, describe las concepciones de un grupo de setenta y ocho profesores de inglés de los niveles de Educación Media General y Educación Universitaria de la ciudad de San Cristóbal, Venezuela, sobre la expansión, uso y enseñanza del inglés como idioma internacional. Desde una perspectiva crítica y posestructuralista en la que se concibe al inglés como un fenómeno social cargado de ideologías y valores culturales, este estudio pretende dar cuenta de las conexiones existentes entre el inglés y las fuerzas económicas, políticas, sociales e ideológicas que subyacen detrás de su expansión, uso y enseñanza en el mundo (Holliday, 2005, 2006, 2013; Pennycook, 1994; Phillipson, 1992, 2010), y la forma en como estas conexiones son percibidas por los profesores de inglés de un contexto específico de la periferia (Canagarajah, 1999a). Para la exploración en profundidad del fenómeno se adopta en este estudio el método fenomenológico (Colás, 1998b) como propuesta metodológica para descubrir la forma en que los profesores de inglés de este contexto específico comprenden el fenómeno y conciben la expansión, uso y enseñanza del inglés como idioma internacional. Como instrumentos para la recolección de los datos se utilizan dos cuestionarios con preguntas abiertas y cerradas administrados por internet a los participantes, y entrevistas semi-estructuradas cara a cara en profundidad con nueve profesores seleccionados como informantes clave. Para el análisis de los datos se toma el proceso analítico de Strauss y Corbin (2002), proceso que apunta hacia la generación inductiva de teoría a través de la comparación constante de los datos para formar categorías. En este estudio, el análisis de los datos comprende un proceso integral y cíclico que toma en cuenta los datos provenientes de todos los participantes y de todos los instrumentos de recolección. También se hace uso técnico del software Atlas.ti versión 6.0 para el almacenamiento y xivmanipulación sistemática de las entrevistas semi-estructuradas en profundidad. Entre los hallazgos se devela una complejidad enmarcada en el estudio de las concepciones de los profesores de este contexto sociocultural específico que resultan ser fluidas y en oportunidades simultáneamente contradictorias entre sí sobre la expansión, uso y enseñanza del inglés como idioma internacional. En tal sentido, los resultados reflejan poca conciencia crítica de los participantes sobre la hegemonía del inglés en el mundo y su percepción del idioma como un asunto necesario y beneficioso para la comunicación internacional, de allí que consideren su enseñanza y aprendizaje como necesarios en todos los contextos del mundo. Este estudio también expresa una concientización de los participantes en algunos aspectos específicos como su sentido de propiedad del idioma y su percepción como hablantes y docentes competentes del inglés en un contexto específico. Asimismo, revela algunos asuntos sociopolíticos enmarcados en la dicotomía 'nativo' - 'no nativo' del inglés fundamentado en el concepto del 'native speakerism'. Al mismo tiempo, muestra la concepción de los participantes sobre la enseñanza del inglés como una actividad apolítica y neutral aunque simultáneamente refleja su inclinación hacia la promoción del pensamiento crítico y reflexivo de los estudiantes a través de sus clases de inglés