988 resultados para correlação genética


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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Genética e Melhoramento Animal - FCAV

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Pós-graduação em Genética - IBILCE

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Genética e Melhoramento Animal - FCAV

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The aim of this study was to analyse the genetic diversity and agronomic performance of a group of advanced and superior strains of soybean, derived from biparental, four-way and eight-way crosses, in order to identify future promising and superior combinations. The experiment was carried out in the experimental area of the Paulista State University, Julio de Mesquita Filho, Jaboticabal Campus, in the state of Sao Paulo, Brazil. The characteristics under evaluation were: number of days to flowering, plant height at flowering, number of days to maturity, plant height at maturity, height of the first pod, number of branches, number of pods per plant, agronomic value, lodging, one hundred seed weight and seed yield., The Mahalanobis distance and the relative contribution of each characteristic were used to calculate the phenotypic distances. Among the genotypes analysed, 19 strains displayed high productivity, being superior to the controls (V-max, CD 216, CD 219 and Conquista). The greatest distance found was between strains JAB 41 and JAB 17 (279.81), followed by JAB 40 and JAB 17 (261.38) and between JAB 40 and JAB 22 (255.46). There were six groups formed using the Ward method, indicating the presence of genetic variability among the tested strains. Increasing the number of parents had no effect on the increase in genetic diversity between the strains, and was not the factor responsible for the grouping or not of the genotypes under test.

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One of the greatest challenges for the agricultural system is to establish agricultural production combined with the conservation of genetic resources, mainly aiming to protect the Permanent Preservation Areas. In this context, mulungu ( Erythrina velutina Willd), among other native species, has been suffering with anthropogenic pressures in various ecosystems, causing reductions in its genetic basis. This work aims to identify ecological and genetic population parameters as indicators of sustainability in two natural populations of mulungu, located in riparian forest, in the state of Sergipe, and to assess the tendency to their sustainability, aiming genetic conservation of the species. The matrix of Pressure-State-Impact/Effect-Response (PEI/ER) was used with the selection of 13 indicators, from the use of RAPDmolecular markers and biochemical (enzymes) markers in populations, in order to present them as relevant information to measure progress as for sustainability and conservation ofmulungu. The studied populations presented low tendency to sustainability, requiring strategies to change this status.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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It is know that endotoxin and various matrix metalloproteinases (MMPs) are involved in the development of periapical lesions. The purpose of this study was to evaluate and correlate the presence of endotoxins and MMP- 3, MMP-8 and MMP-9 in root canals of teeth with necrotic pulp and periapical lesion before, during and after the biomechanical preparation (PBM) using a combination of different irrigations solutions and intracanal dressing. Thirty-three single-root teeth with a diagnosis of pulp necrosis and periapical lesion radiographically visible were selected. Immediately after the coronal opening was collected the first sample from the root canal content. Then, all canals were prepared (cervical and middle thirds) by oscillatory instruments (EndoEze) and irrigated by 2.5% NaOCl. After, a manual preparation was made for the apical third and the teeth were divided into three groups according to the irrigation protocol: G1) 2.5% NaOCl (4 manual files); G2) 2.5% NaOCl (2 manual files) + [Ca (OH)2 0.14%] (2 manual files) and G3) 2.5% NaOCl (2 manual files) + polymyxin B (2 manual files). After the PBM, the second sample was collected; then the third collect was performed after using EDTA final flush. The fourth sample was collected 14 days after placing the dressing [2% chlorhexidine gel + Ca(OH)2]. Quantification of endotoxins was performed by a kinetic chromogenic lysate from amoebocytes of Limulus (LAL) and quantification of MMPs by ELISA assay. The results were analyzed statistically by Kruskal-Wallis and Dunn's test (5%) and ordinal Spearman correlation. Presence of endotoxin was observed in 100% of cases and G3 showed the greatest reduction of endotoxins from the 1st to the 2nd samples (97%), being statistically similar to G2 (84.2%) and different from G1 (49.4%) (p<0.05). The intracanal dressing promoted a significant reduction of endotoxin, no difference among the groups. For...