Could the acid-base status of Antarctic sea urchins indicate a better-than-expected resilience to near-future ocean acidification?

Increasing atmospheric carbon dioxide concentration alters the chemistry of the oceans towards more acidic conditions. Polar oceans are particularly affected due to their low temperature, low carbonate content and mixing patterns, for instance upwellings. Calcifying organisms are expected to be highly impacted by the decrease in the oceans' pH and carbonate ions concentration. In particular, sea urchins, members of the phylum Echinodermata, are hypothesized to be at risk due to their high-magnesium calcite skeleton. However, tolerance to ocean acidification in metazoans is first linked to acid-base regulation capacities of the extracellular fluids. No information on this is available to date for Antarctic echinoderms and inference from temperate and tropical studies needs support. In this study, we investigated the acid-base status of 9 species of sea urchins (3 cidaroids, 2 regular euechinoids and 4 irregular echinoids). It appears that Antarctic regular euechinoids seem equipped with similar acid-base regulation systems as tropical and temperate regular euechinoids but could rely on more passive ion transfer systems, minimizing energy requirements. Cidaroids have an acid-base status similar to that of tropical cidaroids. Therefore Antarctic cidaroids will most probably not be affected by decreasing seawater pH, the pH drop linked to ocean acidification being negligible in comparison of the naturally low pH of the coelomic fluid. Irregular echinoids might not suffer from reduced seawater pH if acidosis of the coelomic fluid pH does not occur but more data on their acid-base regulation are needed. Combining these results with the resilience of Antarctic sea urchin larvae strongly suggests that these organisms might not be the expected victims of ocean acidification. However, data on the impact of other global stressors such as temperature and of the combination of the different stressors needs to be acquired to assess the sensitivity of these organisms to global change.

Chapter 8 Location Choice of Japanese Multinationals: Country Characteristics or Region Characteristics?

In the present global era in which firms choose the location of their plants beyond national borders, location characteristics are important for attracting multinational enterprises (MNEs). The better access to countries with large market is clearly attractive for MNEs. For example, special treatments on tariffs such as the Generalized System of Preferences (GSP) are beneficial for MNEs whose home country does not have such treatments. Not only such country characteristics but also region characteristics (i.e. province-level or city-level ones) matter, particularly in the case that location characteristics differ widely between a nation's regions. The existence of industrial concentration, that is, agglomeration, is a typical regional characteristic. It is with consideration of these country-level and region-level characteristics that MNEs decide their location abroad. A large number of academic studies have investigated in what kinds of countries MNEs locate, i.e. location choice analysis. Employing the usual new economic geography model (i.e. constant elasticity of substitution (CES) utility function, Dixit-Stiglitz monopolistic competition, and ice-berg trade costs), the literature derives the profit function, of which coefficients are estimated using maximum likelihood procedures. Recent studies are as follows: Head, Rise, and Swenson (1999) for Japanese MNEs in the US; Belderbos and Carree (2002) for Japanese MNEs in China; Head and Mayer (2004) for Japanese MNEs in Europe; Disdier and Mayer (2004) for French MNEs in Europe; Castellani and Zanfei (2004) for large MNEs worldwide; Mayer, Mejean, and Nefussi (2007) for French MNEs worldwide; Crozet, Mayer, and Mucchielli (2004) for MNEs in France; and Basile, Castellani, and Zanfei (2008) for MNEs in Europe. At the present time, three main topics can be found in this literature. The first introduces various location elements as independent variables. The above-mentioned new economic geography model usually yields the profit function, which is a function of market size, productive factor prices, price of intermediate goods, and trade costs. As a proxy for the price of intermediate goods, the measure of agglomeration is often used, particularly the number of manufacturing firms. Some studies employ more disaggregated numbers of manufacturing firms, such as the number of manufacturing firms with the same nationality as the firms choosing the location (e.g., Head et al., 1999; Crozet et al., 2004) or the number of firms belonging to the same firm group (e.g., Belderbos and Carree, 2002). As part of trade costs, some investment climate measures have been examined: free trade zones in the US (Head et al., 1999), special economic zones and opening coastal cities in China (Belderbos and Carree, 2002), and Objective 1 structural funds and cohesion funds in Europe (Basile et al., 2008). Second, the validity of proxy variables for location elements is further examined. Head and Mayer (2004) examine the validity of market potential on location choice. They propose the use of two measures: the Harris market potential index (Harris, 1954) and the Krugman-type index used in Redding and Venables (2004). The Harris-type index is simply the sum of distance-weighted real GDP. They employ the Krugman-type market potential index, which is directly derived from the new economic geography model, as it takes into account the extent of competition (i.e. price index) and is constructed using estimators of importing country dummy variables in the well-known gravity equation, as in Redding and Venables (2004). They find that "theory does not pay", in the sense that the Harris market potential outperforms Krugman's market potential in both the magnitude of its coefficient and the fit of the model to be estimated. The third topic explores the substitution of location by examining inclusive values in the nested-logit model. For example, using firm-level data on French investments both in France and abroad over the 1992-2002 period, Mayer et al. (2007) investigate the determinants of location choice and assess empirically whether the domestic economy has been losing attractiveness over the recent period or not. The estimated coefficient for inclusive value is strongly significant and near unity, indicating that the national economy is not different from the rest of the world in terms of substitution patterns. Similarly, Disdier and Mayer (2004) investigate whether French MNEs consider Western and Eastern Europe as two distinct groups of potential host countries by examining the coefficient for the inclusive value in nested-logit estimation. They confirm the relevance of an East-West structure in the country location decision and furthermore show that this relevance decreases over time. The purpose of this paper is to investigate the location choice of Japanese MNEs in Thailand, Cambodia, Laos, Myanmar, and Vietnam, and is closely related to the third topic mentioned above. By examining region-level location choice with the nested-logit model, I investigate the relative importance of not only country characteristics but also region characteristics. Such investigation is invaluable particularly in the case of location choice in those five countries: industrialization remains immature in those countries which have not yet succeeded in attracting enough MNEs, and as a result, it is expected that there are not yet crucial regional variations for MNEs within such a nation, meaning the country characteristics are still relatively important to attract MNEs. To illustrate, in the case of Cambodia and Laos, one of the crucial elements for Japanese MNEs would be that LDC preferential tariff schemes are available for exports from Cambodia and Laos. On the other hand, in the case of Thailand and Vietnam, which have accepted a relatively large number of MNEs and thus raised the extent of regional inequality, regional characteristics such as the existence of agglomeration would become important elements in location choice. Our sample countries seem, therefore, to offer rich variations for analyzing the relative importance between country characteristics and region characteristics. Our empirical strategy has a further advantage. As in the third topic in the location choice literature, the use of the nested-logit model enables us to examine substitution patterns between country-based and region-based location decisions by MNEs in the concerned countries. For example, it is possible to investigate empirically whether Japanese multinational firms consider Thailand/Vietnam and the other three countries as two distinct groups of potential host countries, by examining the inclusive value parameters in nested-logit estimation. In particular, our sample countries all experienced dramatic changes in, for example, economic growth or trade costs reduction during the sample period. Thus, we will find the dramatic dynamics of such substitution patterns. Our rigorous analysis of the relative importance between country characteristics and region characteristics is invaluable from the viewpoint of policy implications. First, while the former characteristics should be improved mainly by central government in each country, there is sometimes room for the improvement of the latter characteristics by even local governments or smaller institutions such as private agencies. Consequently, it becomes important for these smaller institutions to know just how crucial the improvement of region characteristics is for attracting foreign companies. Second, as economies grow, country characteristics become similar among countries. For example, the LCD preferential tariff schemes are available only when a country is less developed. Therefore, it is important particularly for the least developed countries to know what kinds of regional characteristics become important following economic growth; in other words, after their country characteristics become similar to those of the more developed countries. I also incorporate one important characteristic of MNEs, namely, productivity. The well-known Helpman-Melitz-Yeaple model indicates that only firms with higher productivity can afford overseas entry (Helpman et al., 2004). Beyond this argument, there may be some differences in MNEs' productivity among our sample countries and regions. Such differences are important from the viewpoint of "spillover effects" from MNEs, which are one of the most important results for host countries in accepting their entry. The spillover effects are that the presence of inward foreign direct investment (FDI) aises domestic firms' productivity through various channels such as imitation. Such positive effects might be larger in areas with more productive MNEs. Therefore, it becomes important for host countries to know how much productive firms are likely to invest in them. The rest of this paper is organized as follows. Section 2 takes a brief look at the worldwide distribution of Japanese overseas affiliates. Section 3 provides an empirical model to examine their location choice, and lastly, we discuss future works to estimate our model.

Modelling the Impact of Built Environment, Geographical Scales and Latent Constructs On Individual Travel Behaviour

La relación entre la estructura urbana y la movilidad ha sido estudiada desde hace más de 70 años. El entorno urbano incluye múltiples dimensiones como por ejemplo: la estructura urbana, los usos de suelo, la distribución de instalaciones diversas (comercios, escuelas y zonas de restauración, parking, etc.). Al realizar una revisión de la literatura existente en este contexto, se encuentran distintos análisis, metodologías, escalas geográficas y dimensiones, tanto de la movilidad como de la estructura urbana. En este sentido, se trata de una relación muy estudiada pero muy compleja, sobre la que no existe hasta el momento un consenso sobre qué dimensión del entorno urbano influye sobre qué dimensión de la movilidad, y cuál es la manera apropiada de representar esta relación. Con el propósito de contestar estas preguntas investigación, la presente tesis tiene los siguientes objetivos generales: (1) Contribuir al mejor entendimiento de la compleja relación estructura urbana y movilidad. y (2) Entender el rol de los atributos latentes en la relación entorno urbano y movilidad. El objetivo específico de la tesis es analizar la influencia del entorno urbano sobre dos dimensiones de la movilidad: número de viajes y tipo de tour. Vista la complejidad de la relación entorno urbano y movilidad, se pretende contribuir al mejor entendimiento de la relación a través de la utilización de 3 escalas geográficas de las variables y del análisis de la influencia de efectos inobservados en la movilidad. Para el análisis se utiliza una base de datos conformada por tres tipos de datos: (1) Una encuesta de movilidad realizada durante los años 2006 y 2007. Se obtuvo un total de 943 encuestas, en 3 barrios de Madrid: Chamberí, Pozuelo y Algete. (2) Información municipal del Instituto Nacional de Estadística: dicha información se encuentra enlazada con los orígenes y destinos de los viajes recogidos en la encuesta. Y (3) Información georeferenciada en Arc-GIS de los hogares participantes en la encuesta: la base de datos contiene información respecto a la estructura de las calles, localización de escuelas, parking, centros médicos y lugares de restauración. Se analizó la correlación entre e intra-grupos y se modelizaron 4 casos de atributos bajo la estructura ordinal logit. Posteriormente se evalúa la auto-selección a través de la estimación conjunta de las elecciones de tipo de barrio y número de viajes. La elección del tipo de barrio consta de 3 alternativas: CBD, Urban y Suburban, según la zona de residencia recogida en las encuestas. Mientras que la elección del número de viajes consta de 4 categorías ordinales: 0 viajes, 1-2 viajes, 3-4 viajes y 5 o más viajes. A partir de la mejor especificación del modelo ordinal logit. Se desarrolló un modelo joint mixed-ordinal conjunto. Los resultados indican que las variables exógenas requieren un análisis exhaustivo de correlaciones con el fin de evitar resultados sesgados. ha determinado que es importante medir los atributos del BE donde se realiza el viaje, pero también la información municipal es muy explicativa de la movilidad individual. Por tanto, la percepción de las zonas de destino a nivel municipal es considerada importante. En el contexto de la Auto-selección (self-selection) es importante modelizar conjuntamente las decisiones. La Auto-selección existe, puesto que los parámetros estimados conjuntamente son significativos. Sin embargo, sólo ciertos atributos del entorno urbano son igualmente importantes sobre la elección de la zona de residencia y frecuencia de viajes. Para analizar la Propensión al Viaje, se desarrolló un modelo híbrido, formado por: una variable latente, un indicador y un modelo de elección discreta. La variable latente se denomina “Propensión al Viaje”, cuyo indicador en ecuación de medida es el número de viajes; la elección discreta es el tipo de tour. El modelo de elección consiste en 5 alternativas, según la jerarquía de actividades establecida en la tesis: HOME, no realiza viajes durante el día de estudio, HWH tour cuya actividad principal es el trabajo o estudios, y no se realizan paradas intermedias; HWHs tour si el individuo reaiza paradas intermedias; HOH tour cuya actividad principal es distinta a trabajo y estudios, y no se realizan paradas intermedias; HOHs donde se realizan paradas intermedias. Para llegar a la mejor especificación del modelo, se realizó un trabajo importante considerando diferentes estructuras de modelos y tres tipos de estimaciones. De tal manera, se obtuvieron parámetros consistentes y eficientes. Los resultados muestran que la modelización de los tours, representa una ventaja sobre la modelización de los viajes, puesto que supera las limitaciones de espacio y tiempo, enlazando los viajes realizados por la misma persona en el día de estudio. La propensión al viaje (PT) existe y es específica para cada tipo de tour. Los parámetros estimados en el modelo híbrido resultaron significativos y distintos para cada alternativa de tipo de tour. Por último, en la tesis se verifica que los modelos híbridos representan una mejora sobre los modelos tradicionales de elección discreta, dando como resultado parámetros consistentes y más robustos. En cuanto a políticas de transporte, se ha demostrado que los atributos del entorno urbano son más importantes que los LOS (Level of Service) en la generación de tours multi-etapas. la presente tesis representa el primer análisis empírico de la relación entre los tipos de tours y la propensión al viaje. El concepto Propensity to Travel ha sido desarrollado exclusivamente para la tesis. Igualmente, el desarrollo de un modelo conjunto RC-Number of trips basado en tres escalas de medida representa innovación en cuanto a la comparación de las escalas geográficas, que no había sido hecha en la modelización de la self-selection. The relationship between built environment (BE) and travel behaviour (TB) has been studied in a number of cases, using several methods - aggregate and disaggregate approaches - and different focuses – trip frequency, automobile use, and vehicle miles travelled and so on. Definitely, travel is generated by the need to undertake activities and obtain services, and there is a general consensus that urban components affect TB. However researches are still needed to better understand which components of the travel behaviour are affected most and by which of the urban components. In order to fill the gap in the research, the present dissertation faced two main objectives: (1) To contribute to the better understanding of the relationship between travel demand and urban environment. And (2) To develop an econometric model for estimating travel demand with urban environment attributes. With this purpose, the present thesis faced an exhaustive research and computation of land-use variables in order to find the best representation of BE for modelling trip frequency. In particular two empirical analyses are carried out: 1. Estimation of three dimensions of travel demand using dimensions of urban environment. We compare different travel dimensions and geographical scales, and we measure self-selection contribution following the joint models. 2. Develop a hybrid model, integrated latent variable and discrete choice model. The implementation of hybrid models is new in the analysis of land-use and travel behaviour. BE and TB explicitly interact and allow richness information about a specific individual decision process For all empirical analysis is used a data-base from a survey conducted in 2006 and 2007 in Madrid. Spatial attributes describing neighbourhood environment are derived from different data sources: National Institute of Statistics-INE (Administrative: municipality and district) and GIS (circular units). INE provides raw data for such spatial units as: municipality and district. The construction of census units is trivial as the census bureau provides tables that readily define districts and municipalities. The construction of circular units requires us to determine the radius and associate the spatial information to our households. The first empirical part analyzes trip frequency by applying an ordered logit model. In this part is studied the effect of socio-economic, transport and land use characteristics on two travel dimensions: trip frequency and type of tour. In particular the land use is defined in terms of type of neighbourhoods and types of dwellers. Three neighbourhood representations are explored, and described three for constructing neighbourhood attributes. In particular administrative units are examined to represent neighbourhood and circular – unit representation. Ordered logit models are applied, while ordinal logit models are well-known, an intensive work for constructing a spatial attributes was carried out. On the other hand, the second empirical analysis consists of the development of an innovative econometric model that considers a latent variable called “propensity to travel”, and choice model is the choice of type of tour. The first two specifications of ordinal models help to estimate this latent variable. The latent variable is unobserved but the manifestation is called “indicators”, then the probability of choosing an alternative of tour is conditional to the probability of latent variable and type of tour. Since latent variable is unknown we fit the integral over its distribution. Four “sets of best variables” are specified, following the specification obtained from the correlation analysis. The results evidence that the relative importance of SE variables versus BE variables depends on how BE variables are measured. We found that each of these three spatial scales has its intangible qualities and drawbacks. Spatial scales play an important role on predicting travel demand due to the variability in measures at trip origin/destinations within the same administrative unit (municipality, district and so on). Larger units will produce less variation in data; but it does not affect certain variables, such as public transport supply, that are more significant at municipality level. By contrast, land-use measures are more efficient at district level. Self-selection in this context, is weak. Thus, the influence of BE attributes is true. The results of the hybrid model show that unobserved factors affect the choice of tour complexity. The latent variable used in this model is propensity to travel that is explained by socioeconomic aspects and neighbourhood attributes. The results show that neighbourhood attributes have indeed a significant impact on the choice of the type of tours either directly and through the propensity to travel. The propensity to travel has a different impact depending on the structure of each tour and increases the probability of choosing more complex tours, such as tours with many intermediate stops. The integration of choice and latent variable model shows that omitting important perception and attitudes leads to inconsistent estimates. The results also indicate that goodness of fit improves by adding the latent variable in both sequential and simultaneous estimation. There are significant differences in the sensitivity to the latent variable across alternatives. In general, as expected, the hybrid models show a major improvement into the goodness of fit of the model, compared to a classical discrete choice model that does not incorporate latent effects. The integrated model leads to a more detailed analysis of the behavioural process. Summarizing, the effect that built environment characteristics on trip frequency studied is deeply analyzed. In particular we tried to better understand how land use characteristics can be defined and measured and which of these measures do have really an impact on trip frequency. We also tried to test the superiority of HCM on this field. We can concluded that HCM shows a major improvement into the goodness of fit of the model, compared to classical discrete choice model that does not incorporate latent effects. And consequently, the application of HCM shows the importance of LV on the decision of tour complexity. People are more elastic to built environment attributes than level of services. Thus, policy implications must take place to develop more mixed areas, work-places in combination with commercial retails.

Next Generation of Energy Residential Gateways for Demand Response and Dynamic Pricing

Predictions about electric energy needs, based on current electric energy models, forecast that the global energy consumption on Earth for 2050 will double present rates. Using distributed procedures for control and integration, the expected needs can be halved. Therefore implementation of Smart Grids is necessary. Interaction between final consumers and utilities is a key factor of future Smart Grids. This interaction is aimed to reach efficient and responsible energy consumption. Energy Residential Gateways (ERG) are new in-building devices that will govern the communication between user and utility and will control electric loads. Utilities will offer new services empowering residential customers to lower their electric bill. Some of these services are Smart Metering, Demand Response and Dynamic Pricing. This paper presents a practical development of an ERG for residential buildings.

Estrategias innovadoras para la gestión del riesgo de sequía en sistemas ganaderos extensivos

La vulnerabilidad de los sistemas ganaderos de pastoreo pone en evidencia la necesidad de herramientas para evaluar y mitigar los efectos de la sequía. El avance en la teledetección ha despertado el interés por explotar potenciales aplicaciones, y está dando lugar a un intenso desarrollo de innovaciones en distintos campos. Una de estas áreas es la gestión del riesgo climático, en donde la utilización de índices de vegetación permite la evaluación de la sequía. En esta investigación, se analiza el impacto de la sequía y se evalúa el potencial de nuevas tecnologías como la teledetección para la gestión del riesgo de sequía en sistemas de ganadería extensiva. Para ello, se desarrollan tres aplicaciones: (i) evaluar el impacto económico de la sequía en una explotación ganadera extensiva de la dehesa de Andalucía, (ii) elaborar mapas de vulnerabilidad a la sequía en pastos de Chile y (iii) diseñar y evaluar el potencial de un seguro indexado para sequía en pastos en la región de Coquimbo en Chile. En la primera aplicación, se diseña un modelo dinámico y estocástico que integra aspectos climáticos, ecológicos, agronómicos y socioeconómicos para evaluar el riesgo de sequía. El modelo simula una explotación ganadera tipo de la dehesa de Andalucía para el período 1999-2010. El método de Análisis Histórico y la simulación de MonteCarlo se utilizan para identificar los principales factores de riesgo de la explotación, entre los que destacan, los periodos de inicios del verano e inicios de invierno. Los resultados muestran la existencia de un desfase temporal entre el riesgo climático y riesgo económico, teniendo este último un periodo de duración más extenso en el tiempo. También, revelan que la intensidad, frecuencia y duración son tres atributos cruciales que determinan el impacto económico de la sequía. La estrategia de reducción de la carga ganadera permite aminorar el riesgo, pero conlleva una disminución en el margen bruto de la explotación. La segunda aplicación está dedicada a la elaboración de mapas de vulnerabilidad a la sequia en pastos de Chile. Para ello, se propone y desarrolla un índice de riesgo económico (IRESP) sencillo de interpretar y replicable, que integra factores de riesgo y estrategias de adaptación para obtener una medida del Valor en Riesgo, es decir, la máxima pérdida esperada en un año con un nivel de significación del 5%.La representación espacial del IRESP pone en evidencia patrones espaciales y diferencias significativas en la vulnerabilidad a la sequía a lo largo de Chile. Además, refleja que la vulnerabilidad no siempre esta correlacionada con el riesgo climático y demuestra la importancia de considerar las estrategias de adaptación. Las medidas de autocorrelación espacial revelan que el riesgo sistémico es considerablemente mayor en el sur que en el resto de zonas. Los resultados demuestran que el IRESP transmite información pertinente y, que los mapas de vulnerabilidad pueden ser una herramienta útil en el diseño de políticas y toma de decisiones para la gestión del riesgo de sequía. La tercera aplicación evalúa el potencial de un seguro indexado para sequía en pastos en la región de Coquimbo en Chile. Para lo cual, se desarrolla un modelo estocástico para estimar la prima actuarialmente justa del seguro y se proponen y evalúan pautas alternativas para mejorar el diseño del contrato. Se aborda el riesgo base, el principal problema de los seguros indexados identificado en la literatura y, que está referido a la correlación imperfecta del índice con las pérdidas de la explotación. Para ello, se sigue un enfoque bayesiano que permite evaluar el impacto en el riesgo base de las pautas de diseño propuestas: i) una zonificación por clúster que considera aspectos espacio-temporales, ii) un período de garantía acotado a los ciclos fenológicos del pasto y iii) umbral de garantía. Los resultados muestran que tanto la zonificación como el periodo de garantía reducen el riesgo base considerablemente. Sin embargo, el umbral de garantía tiene un efecto ambiguo sobre el riesgo base. Por otra parte, la zonificación por clúster contribuye a aminorar el riesgo sistémico que enfrentan las aseguradoras. Estos resultados han puesto de manifiesto que un buen diseño de contrato puede tener un doble dividendo, por un lado aumentar su utilidad y, por otro, reducir el coste del seguro. Un diseño de contrato eficiente junto con los avances en la teledetección y un adecuado marco institucional son los pilares básicos para el buen funcionamiento de un programa de seguro. Las nuevas tecnologías ofrecen un importante potencial para la innovación en la gestión del riesgo climático. Los avances en este campo pueden proporcionar importantes beneficios sociales en los países en desarrollo y regiones vulnerables, donde las herramientas para gestionar eficazmente los riesgos sistémicos como la sequía pueden ser de gran ayuda para el desarrollo. The vulnerability of grazing livestock systems highlights the need for tools to assess and mitigate the adverse impact of drought. The recent and rapid progress in remote sensing has awakened an interest for tapping into potential applications, triggering intensive efforts to develop innovations in a number of spheres. One of these areas is climate risk management, where the use of vegetation indices facilitates assessment of drought. This research analyzes drought impacts and evaluates the potential of new technologies such as remote sensing to manage drought risk in extensive livestock systems. Three essays in drought risk management are developed to: (i) assess the economic impact of drought on a livestock farm in the Andalusian Dehesa, (ii) build drought vulnerability maps in Chilean grazing lands, and (iii) design and evaluate the potential of an index insurance policy to address the risk of drought in grazing lands in Coquimbo, Chile. In the first essay, a dynamic and stochastic farm model is designed combining climate, agronomic, socio-economic and ecological aspects to assess drought risk. The model is developed to simulate a representative livestock farm in the Dehesa of Andalusia for the time period 1999-2010. Burn analysis and MonteCarlo simulation methods are used to identify the significance of various risk sources at the farm. Most notably, early summer and early winter are identified as periods of peak risk. Moreover, there is a significant time lag between climate and economic risk and this later last longer than the former. It is shown that intensity, frequency and duration of the drought are three crucial attributes that shape the economic impact of drought. Sensitivity analysis is conducted to assess the sustainability of farm management strategies and demonstrates that lowering the stocking rate reduces farmer exposure to drought risk but entails a reduction in the expected gross margin. The second essay, mapping drought vulnerability in Chilean grazing lands, proposes and builds an index of economic risk (IRESP) that is replicable and simple to interpret. This methodology integrates risk factors and adaptation strategies to deliver information on Value at Risk, maximum expected losses at 5% significance level. Mapping IRESP provides evidence about spatial patterns and significant differences in drought vulnerability across Chilean grazing lands. Spatial autocorrelation measures reveal that systemic risk is considerably larger in the South as compared to Northern or Central Regions. Furthermore, it is shown that vulnerability is not necessarily correlated with climate risk and that adaptation strategies do matter. These results show that IRESP conveys relevant information and that vulnerability maps may be useful tools to assess policy design and decision-making in drought risk management. The third essay develops a stochastic model to estimate the actuarially fair premium and evaluates the potential of an indexed insurance policy to manage drought risk in Coquimbo, a relevant livestock farming region of Chile. Basis risk refers to the imperfect correlation of the index and farmer loses and is identified in the literature as a main limitation of index insurance. A Bayesian approach is proposed to assess the impact on basis risk of alternative guidelines in contract design: i) A cluster zoning that considers space-time aspects, ii) A guarantee period bounded to fit phenological cycles, and iii) the triggering index threshold. Results show that both the proposed zoning and guarantee period considerably reduces basis risk. However, the triggering index threshold has an ambiguous effect on basis risk. On the other hand, cluster zoning contributes to ameliorate systemic risk faced by the insurer. These results highlighted that adequate contract design is important and may result in double dividend. On the one hand, increasing farmers’ utility and, secondly, reducing the cost of insurance. An efficient contract design coupled with advances in remote sensing and an appropriate institutional framework are the basis for an efficient operation of an insurance program. The new technologies offer significant potential for innovation in climate risk managements. Progress in this field is capturing increasing attention and may provide important social gains in developing countries and vulnerable regions where the tools to efficiently manage systemic risks, such as drought, may be a means to foster development.

Conditional parallelization of nonStrict independence. Procedures and assessmen

This paper presents a conditional parallelization process for and-parallelism based on the notion of non-strict independence, a more relaxed notion than the traditional of strict independence. By using this notion, a parallelism annotator can extract more parallelism from programs. On the other hand, the intrinsic complexity of non-strict independence poses new challenges to this task. We report here on the implementation we have accomplished of an annotator for non-strict independence, capable of producing both static and dynamic execution graphs. This implementation, along with the also implemented independence checker and their integration in our system, have resulted what is, to the best of our knowledge, the first parallelizing compiler based on nonstrict independence which produces dynamic execution graphs. The paper also presents a preliminary assessment of the implemented tools, comparing them with the existing ones for strict independence, which shows encouraging results.

Human midbrain precursors activate the expected developmental genetic program and differentiate long-term to functional A9 dopamine neurons in vitro. Enhancement by Bcl-XL

Understanding the molecular programs of the generation of human dopaminergic neurons (DAn) from their ventral mesencephalic (VM) precursors is of key importance for basic studies, progress in cell therapy, drug screening and pharmacology in the context of Parkinson's disease. The nature of human DAn precursors in vitro is poorly understood, their properties unstable, and their availability highly limited. Here we present positive evidence that human VM precursors retaining their genuine properties and long-term capacity to generate A9 type Substantia nigra human DAn (hVM1 model cell line) can be propagated in culture. During a one month differentiation, these cells activate all key genes needed to progress from pro-neural and prodopaminergic precursors to mature and functional DAn. For the first time, we demonstrate that gene cascades are correctly activated during differentiation, resulting in the generation of mature DAn. These DAn have morphological and functional properties undistinguishable from those generated by VM primary neuronal cultures. In addition, we have found that the forced expression of Bcl-XL induces an increase in the expression of key developmental genes (MSX1, NGN2), maintenance of PITX3 expression temporal profile, and also enhances genes involved in DAn long-term function, maintenance and survival (EN1, LMX1B, NURR1 and PITX3). As a result, Bcl-XL anticipates and enhances DAn generation.

The new cloud computing paradigm: the way to IT seen as a utility

In the present competitive environment, companies are wondering how to reduce their IT costs while increasing their efficiency and agility to react when changes in the business processes are required. Cloud Computing is the latest paradigm to optimize the use of IT resources considering ?everything as a service? and receiving these services from the Cloud (Internet) instead of owning and managing hardware and software assets. The benefits from the model are clear. However, there are also concerns and issues to be solved before Cloud Computing spreads across the different industries. This model will allow a pay-per-use model for the IT services and many benefits like cost savings, agility to react when business demands changes and simplicity because there will not be any infrastructure to operate and administrate. It will be comparable to the well known utilities like electricity, water or gas companies. However, this paper underlines several risk factors of the model. Leading technology companies should research on solutions to minimize the risks described in this article. Keywords - Cloud Computing, Utility Computing, Elastic Computing, Enterprise Agility

Preference intensity in MCDM when an additive utility function represents DM preferences

We propose a new method for ranking alternatives in multicriteria decision-making problems when there is imprecision concerning the alternative performances, component utility functions and weights. We assume decision maker?s preferences are represented by an additive multiattribute utility function, in which weights can be modeled by independent normal variables, fuzzy numbers, value intervals or by an ordinal relation. The approaches are based on dominance measures or exploring the weight space in order to describe which ratings would make each alternative the preferred one. On the one hand, the approaches based on dominance measures compute the minimum utility difference among pairs of alternatives. Then, they compute a measure by which to rank the alternatives. On the other hand, the approaches based on exploring the weight space compute confidence factors describing the reliability of the analysis. These methods are compared using Monte Carlo simulation.

Determinación del contenido de alúmina reactiva de las adiciones puzolánicas naturales y artificiales, por termogravimetría y por el método de Rietveld

Tanto el tema de esta investigación, como sus objetivos, fundamentos, materiales seleccionados y parte experimental para alcanzarlos, han sido promovidos por la situación y el estado de las construcciones de hormigón armado que se comenzaron a realizar en España a partir del año 1975, con hormigones y morteros de cemento que contenían cenizas volantes hasta un 20 %, en principio, y hasta un 35 %, más tarde, de su dosificación de cemento Portland (CP), los cuales y en contra de lo esperado, han demandado y continúan demandando, muy a pesar de sus aparentes bondades de todo tipo que se les atribuían, una necesidad de mejora de sus prestaciones, especialmente, debido a un nivel inesperadamente bajo de la durabilidad de algunas obras construidas con los mismos. Todo era debido, en definitiva, a que las adiciones puzolánicas, naturales y artificiales, tales como las cenizas volantes, referidas antes, se vienen utilizando reglamentariamente para la fabricación de cementos y/o de sus productos derivados, hormigones, morteros y pastas, en la mayor parte de los países industrializados, desde hace ya más de tres décadas aproximadamente, en las mismas condiciones e idénticos usos constructivos que los hormigones y morteros de CP puro, viniendo además, dictada dicha utilización de estos materiales residuales, hoy sub-productos industriales, por cuestiones medioambientales y/o económicas, principalmente, motivo por el cual esta Tesis Doctoral ha pretendido responder también, adecuadamente y de manera esquemática (en forma de diagrama de flujo), a los criterios que deben de tenerse en cuenta a la hora de su selección y caracterización normalizada y reglamentada de estas adiciones minerales activas, sobre todo, antes de su dosificación y uso en forma del denominado cemento Portland con puzolana, o con ceniza volante, o con esquistos calcinados o con puzolanas calcinadas o con humo de sílice, cemento Portland mixto, cemento puzolánico o cemento compuesto, para que dichos problemas no se le produzcan al hormigón armado ni en masa. De aquí el enfoque tan particular y especial de esta investigación, al haberla circunscrito únicamente a las puzolanas naturales y artificiales, por considerarlas todas ellas, independientemente de por su origen, como materiales constituidos por pequeñas fracciones cristalinas distribuidas aleatoriamente en una matriz mayoritariamente vítrea y/o amorfa, la cual es la que le confiere su reactividad con el hidróxido de calcio en forma de cal apagada o de portlandita procedente de la hidratación del CP. A su vez, dichas fracciones vítreas y/o amorfas están constituidas, en su mayor parte, por sílice reactiva, SiO2r-, alúmina reactiva, Al2O3r-, y óxido de hierro reactivo, Fe2O3r-, recibiendo además, en conjunto, el nombre específico de "factores hidráulicos" de la puzolana, los cuales, por lo común, difieren cuantitativamente de sus contenidos totales, determinados por fusión alcalina mediante procedimientos normalizados. De los tres óxidos reactivos mencionados y desde el punto de vista técnico, los más importantes por su mayor presencia en casi todas las puzolanas naturales y artificiales y, también, transcendencia en la durabilidad química que les pueden llegar a conferir al hormigón armado y en masa, mortero o pasta de cemento que las contenga, son la SiO2r- y la Al2O3r-. El primero de los dos, la SiO2r-, reacciona nada más que con la portlandita (y el Ca(OH)2) para formar geles C-S-H, más tarde transformados en tobermoritas o en jennitas, semejantes a los que originan la alita y la belita del CP en su hidratación. Y desde el punto de vista normativo, la presencia de esta fracción silícica reactiva en las puzolanas viene regulada por la norma EN 197-1, de modo general, siendo además referida por la norma EN 450-1:2006, en el contexto de las cenizas volantes en su adición al hormigón, como "un polvo fino de partículas principalmente de forma esférica y cristalina, procedentes de la combustión de carbón pulverizado, que tiene propiedades puzolánicas y que está compuesto fundamentalmente de SiO2 y Al2O3". Además y de acuerdo con la primera de las dos normas, "El contenido de dióxido de silicio reactivo (definido y determinado según la norma EN 196-2 o su equivalente la UNE 80225) no debe ser inferior al 25 % en masa". Por su parte, cuantiosos estudios experimentales realizados por Talero solo y con otros investigadores, han demostrado que si la puzolana no es adecuada en calidad ni en cantidad, la durabilidad del cemento del que forme parte, y, por consiguiente, de los productos derivados que con él se fabriquen, hormigones, morteros, pastas y prefabricados, puede llegar a ser anormalmente baja, porque la alúmina reactiva, Al2O3r-, o alúmina tetra- o penta-coordinada que la constituye, se implica como tal, de una forma muy directa y con resultados totalmente contrapuestos incluso, en los ataques químicos agresivos naturales que se les producen, provenientes de terrenos y aguas selenitosas (sulfatos, que atacan en su caso al propio material hormigón, mortero y pasta que la contiene para formar ettringita de rápida formación, ett-rf, la cual puede llegar incluso a provocar un ataque rápido del yeso), del rocío marino y de las sales de deshielo (cloruros, que atacan, en cambio, a las armaduras de acero del hormigón provocándoles su corrosión electroquímica por "picadura", si bien, en este otro ataque químico, dicha Al2O3r- lo que origina es sal de Friedel de rápida formación, sF-rf, también, cuyo efecto es, en cambio, colmatador y protector, en definitiva, de dicha corrosión electroquímica), del agua de mar (acción agresiva mutua de cloruros y sulfatos), de la carbonatación, de la reactividad árido-álcali, además de intervenir en la liberación del calor de hidratación, así como también, en el comportamiento reológico de sus pastas, etc., acortándoles de este modo su durabilidad prevista y, en ocasiones, muy seriamente incluso. Pero lo más paradójico de todo es, que a pesar de su referido comportamiento totalmente contrapuesto, frente a sulfatos y cloruros, - aún no se dispone de un método de análisis químico para su determinación cuantitativa, que sea además relativamente sencillo en su operatividad, veraz, preciso, de respuesta rápida, desde el punto de vista técnico de la construcción (no más de 28 días), repetible, reproducible, que no implique peligro alguno para la seguridad vital de las personas que lo tengan que manipular y poner en práctica, económico, y que sirva también tanto para investigación -vertiente científica-, como, sobre todo, para control de calidad -vertiente técnica-, - y ni mucho menos tampoco se dispone todavía, de especificación química alguna que precise el contenido máximo de Al2O3r- (%) que tiene que poseer una puzolana natural o artificial, para poder ser añadida al cemento Portland y/o al hormigón que va a estar sometido a un determinado ataque químico agresivo de los mencionados antes, y, en especial, a sulfatos, a cloruros o al agua de mar. Y para mayor justificación de ambas necesidades, se ha de decir también que la vigente Instrucción de Hormigón Estructural EHE-08 no contempla tampoco especificación química alguna sobre los "factores hidráulicos", en general, ni del contenido de Al2O3r-(%) de las cenizas volantes, muy en particular, en su Artículo 30º "Adiciones", ni en ningún otro Artículo, a pesar de que sí contempla, en cambio, - otras especificaciones químicas que carecen del necesario significado en cuanto a la necesidad de llevar explícita o implícitamente, el mensaje de la Durabilidad Química deseado, y - el Artículo 37º, el cual y para mayor abundamiento se titula paradójicamente "Durabilidad del hormigón y de las armaduras". Asimismo, tampoco se contempla en la última versión que acaba de publicarse de la norma EN 197-1 titulada: "Cementos. Parte 1: Composición, especificaciones y criterios de conformidad de los cementos comunes". Ni tampoco, en la norma EN 450-1:2006 titulada "Cenizas volantes para hormigón. Parte 1: Definiciones, especificaciones y criterios de conformidad", ni en la vigente Instrucción española para la Recepción de Cementos, RC-08, ni en la norma ASTM C618-03 tampoco. La única especificación química que ambas normas, la europea y la norteamericana, refieren es aquella que dice que la suma de los contenidos porcentuales de SiO2 total, Al2O3 total y Fe2O3 total, de la puzolana natural o artificial, ha de ser mayor o igual que 70 %, definiendo, además, a las puzolanas de este modo tan genérico: "materiales silíceos o silíceos y aluminosos, que por sí mismos no poseen valor cementante alguno, pero que finamente divididos y en presencia de humedad, reaccionarán químicamente con hidróxido de calcio a temperaturas ordinarias para formar compuestos que poseen propiedades cementantes". Por consiguiente y de acuerdo con todo lo anterior, el objetivo primordial de esta Tesis Doctoral ha sido: Diseñar y poner a punto un nuevo método analítico de utilidad técnica (que la duración máxima del ensayo no sea mayor de 28 días), para determinar el contenido de alúmina reactiva, vítrea o amorfa, Al2O3r-, de las puzolanas naturales y artificiales. Y una vez puesto a punto, validarlo a nivel de su repetibilidad, de acuerdo con parámetros estadísticos apropiados, poniendo especial énfasis en los criterios de aceptación establecidos por la American Association of Official Analytical Chemists (AOAC). Y para conseguirlo, la innovación de esta investigación se ha basado en los siguientes fundamentos generales, a saber: - Toda la alúmina de cualquier puzolana natural o artificial, capaz de ser atacada, disuelta y lixiviada en 28 días, por la portlandita o por el hidróxido de calcio, Ca(OH)2, en medio acuoso, es considerada como alúmina reactiva, Al2O3r-. - Dicha fracción alumínica reactiva de la puzolana natural o artificial se tiene que encontrar, además, en el estado físico-químico de poder reaccionar químicamente también, en presencia de hidróxido de calcio, cloruro de sodio y agua, para originar monocloro¿aluminato de calcio hidratado, C3A·CaCl2·10H2O, o sal de Friedel. Además, dicho estado físico-químico de la puzolana ha de ser acorde con la definición de alúmina reactiva adoptada en esta investigación en razón de las prestaciones reales de durabilidad química que le puede llegar a conferir a los cementos de mezcla y a sus productos derivados, hormigones, morteros y pastas, que se fabriquen con la misma. - La originalidad de este nuevo método analítico, respecto a los demás métodos ya existentes, reside en que la cuantificación de dicha fracción alumínica reactiva de la puzolana natural o artificial, se realiza mediante cálculo estequiométrico, basándose, para ello, en dicha reacción química de formación de sal de Friedel precisamente, tras 28 días de hidratación básica-salina acelerada de la puzolana natural o artificial, habiéndose realizado, además, en esta investigación dicha determinación cuantitativa de la cantidad de sal de Friedel originada por cada puzolana, mediante dos técnicas analíticas instrumentales que fueron las siguientes: el análisis termogravimétrico (variante I ó I-I en su caso) y el método de Rietveld con la difracción de Rayos X en polvo (variante II). - La reacción química de formación de sal de Friedel tras 28 días de hidratación básica-salina acelerada de las puzolanas que se analicen, se optimizó para asegurar que el único compuesto químico de aluminio y cloro formado fuese sal de Friedel nada más (dosificando para ello en cantidad adecuada los reactivos químicos necesarios: Ca(OH)2, NaCl y agua destilada), manteniendo, además y por otra parte, el compromiso apropiado entre el máximo rendimiento de dicha reacción química (ataque, disolución y lixiviación en 28 días, de toda la alúmina reactiva de la puzolana) y el modo y medios más adecuados de acelerarlo para conseguirlo fue a 40°C de temperatura, con agitación constante y cierre hermético del reactor. - La aplicabilidad y selectividad del nuevo método analítico, objeto de esta Tesis Doctoral, fue estudiada con una serie de puzolanas naturales y artificiales españolas, silíceas y/o silíceas y aluminosas en naturaleza, que fueron las siguientes: M0 (metacaolín 0), M1 (M0 con 50 % de cuarzo), C y L (puzolanas naturales de las Islas Canarias), CV10 y CV17 (cenizas volantes), A (puzolana natural de Almagro), O (puzolana natural de Olot) y HS (humo de sílice). - Todas las adiciones minerales anteriores cumplieron con los principales requisitos físicos y químicos que son preceptivos para poder considerarlas, antes de todo, como puzolanas, lo que era indispensable y de obligado cumplimiento, para poderles determinar su contenido total de Al2O3r- (%) mediante el nuevo método analítico. Estos condicionantes fueron los siguientes: grado adecuado de finura de molido o tamaño medio de partícula (según la norma EN 451-2), haber sido analizadas químicamente antes de todo (según la norma EN 196-2 ó la ASTM C311), con el fin de determinarles especialmente, sus contenidos totales de SiO2 (%), Al2O3 (%) y Fe2O3 (%), haberles determinado, además, su contenido de sílice reactiva, SiO2r- (%) (según la norma UNE 80225), y haber cumplido con el ensayo de puzolanicidad o de Frattini (según la norma EN 196-5) a la edad de 28 días al menos. Este último requisito, otrora de obligado cumplimiento hasta el año 1988, para cualquier puzolana natural y artificial que una fábrica de cementos pretendiera introducir en el proceso de fabricación de un nuevo cemento puzolánico o cemento tipo CEM IV, ha logrado así, que se tenga que volver utilizar de nuevo de forma obligada con esta Tesis Doctoral. Y los resultados obtenidos con el nuevo método analítico de los contenidos de Al2O3r-(%) de las puzolanas seleccionadas, fueron los siguientes: - Mediante su variante I: M0 29.9 %, M1 16.9 %, CV10 11.4 %, L 12.3 %, C 12.6 %, A 8.0 %, CV17 9.5 % y O 6.3 % de Al2O3r-, y - Mediante su variante II: M0 30.7 %, M1 15.4 %, CV10 14.7%, L 11.8 %, C 11.1 %, A 8.9 %, CV17 9.6 % y O 6.8 % de Al2O3r-. Finalmente, todos ellos fueron contrastados, además, mediante la calibración y validación del nuevo método analítico, con los valores de referencia obtenidos de esas mismas puzolanas, los cuales se les habían determinado mediante el método de Florentín, consistente en atacar, disolver y lixiviar también toda la fracción alumínica soluble de la puzolana (y además, aquella silícica y férrica que la constituyen a la par), pero, en especial, su contenido total de alúmina reactiva, mediante un ataque básico (con Ca(OH)2 en medio acuoso a temperatura del laboratorio, habiendo sido, además, su duración hasta 1 año), seguido de otro ácido (con HCl, d = 1.12), habiéndose obtenido esta vez los siguientes resultados de sus contenidos de Al2O3r- (%): M0 28.8 %, M1 16.7 %, CV10 9.7 %, L 11.2 %, C 12.2 %, A 13.0 %, CV17 10.6 % y O 9.5 %. Dicha validación realizada ha puesto de manifiesto, en términos generales, que el nuevo método analítico es más fidedigno que el de Florentín, por lo que resulta ser totalmente apropiado para obtener los resultados que se han pretendido, además de proporcionarlos en un espacio de tiempo relativamente corto (28 días a lo sumo) y a un coste económico razonable por no elevado (salvo error u omisión y libre de impuestos directos e indirectos, el coste económico estimado de la variante I estaría en torno a 800.00 - 900.00 €/puzolana -caso más probable-, y aproximadamente una tercera parte nada más, en el caso de que la edad máxima del ensayo acelerado sea 7 días nada más -caso menos probable-), y, por consiguiente, técnicamente aceptable, al cumplir, además, en todo el rango considerado de variabilidad posible de concentraciones o contenidos del analito buscado en la puzolana, con tales parámetros de validación como son: linealidad (los resultados obtenidos son directamente proporcionales a la señal-respuesta instrumental recibida), sensibilidad, precisión excelente, repetibilidad satisfactoria de los valores obtenidos de los contenidos de Al2O3r- de todas y cada una de las adiciones puzolánicas seleccionadas, confirmando, por ello, la universalidad de su uso. Finalmente, las ventajas del nuevo método analítico, respecto a los métodos ya existentes recopilados de la bibliografía (el método de Florentín, el método de López Ruiz -HF 40 % y HNO3 2N-, el método de Murat y Driouche para arcillas -HF 0.5M-, el método de Arjuan, Silbee y Roy para cenizas volantes -HF 1 %- y su modificación por Fernández-Jiménez y cols. -HF 1 %, 27Al NMR MAS y XRD (método de Rietveld)-, y el método de determinación de la relación SiO2r-/Al2O3r- para arcillas y cenizas volantes por Ruiz-Santaquiteria y cols. -HF 1 %, NaOH 8M y ICP-AES-) son, principalmente, estar exento de peligro alguno para la seguridad vital de las personas que lo tengan que manipular y poner en práctica, ser bastante apropiado para control de calidad además de para investigación, su considerable menor coste económico, su relativamente corto espacio de tiempo que se necesita para obtener la respuesta-resultado pretendida (28 días a lo sumo), así como su universalidad y selectividad, puesto que además, su aplicabilidad es para todo tipo de adiciones puzolánicas naturales o artificiales, como así lo demuestran los resultados obtenidos de los materiales puzolánicos naturales y artificiales seleccionados y analizados, en un rango de concentraciones del analito -contenido de alúmina reactiva, Al2O3r- (%)-, desde el 5 % hasta el 30 % en masa, rango éste que, por otra parte, comprende prácticamente TODAS las adiciones puzolanas naturales y artificiales existentes en el mercado transnacional y las aún por existir. Por consiguiente y de acuerdo con lo anterior, el nuevo método analítico, ya sea realizado mediante su variante I o la II, debido, - en primer lugar, a los fundamentados planteamientos relativos a su procedimiento experimental -modus operandi- el cual ha sido aplicado a una amplia gama de puzolanas naturales y artificiales, y - en segundo lugar, debido a la calidad de los resultados obtenidos con un grado de precisión y repetibilidad excelentes, ha demostrado poseer una gran utilidad científica -para investigación-, pero, sobre todo, técnica -para control de calidad de adiciones puzolánicas naturales y artificiales que se adicionan habitualmente al cemento Portland en fábrica y/o a sus hormigones y morteros en planta-, además de ser representativos los valores obtenidos mediante el mismo respecto a la más que probable durabilidad química que cada una de ellas puede llegarle a conferir al hormigón armado y en masa, mortero y pasta del que forme parte, así como también su cantidad adecuada de sustitución parcial de cada cemento Portland para conseguirla, acorde con sus propias prestaciones químico-físicas o físico-químicas que puede llegarle a conferir, según sea su carácter químico (alumínico, alumínico-silícico, silícico-alumínico, silícico-férrico-alumínico o silícico), forma y tamaño medio de su partícula. Por último, el nuevo método analítico ha demostrado cumplir además, con todos los requisitos de obligado cumplimiento que establece la norma ISO/IEC 17025 sobre la calidad y fiabilidad de nuevos métodos o procedimientos analíticos no normalizados todavía, para poder ser propuesto en un futuro próximo, ante la Comisión de AENOR correspondiente, con objeto de comenzar un expediente para su certificación y normalización. ________________________________________________________________________________ Both the subject of this research, its objectives, fundamentals, materials selected and experimental part to achieve, have all been promoted by the situation and the state of reinforced concrete constructions that began performing in Spain from 1975, with concrete and mortars cement containing fly ash up to 20 %, in principle, and later, up to 35 % to its content of Portland cement, which and against expected, demanded a need to improve their performance, especially due to an unexpectedly low level of durability of some works built with them, despite, however, its apparent benefits of all kinds are ascribed to them. Ultimately, the natural or artificial pozzolanic additions, such as fly ash specially, referred to above, have been used with regulation to manufacture cements and/or its derivatives, concretes, mortars, cement pastes, in the most industrialized countries. More than three decades ago, under the same conditions and identical construction mainly uses concretes and mortars plain Portland cement, besides coming, given that use of these waste materials, industrial by-products today for environmental and/or economic issues. For this reason, this Doctoral Thesis aims to answer properly and schematically (in the form of flow chart), the criteria to be taken into account when selection and characterization standardized for these active mineral additions, especially prior to choosing and use in the so-called Portland Cement (PC) pozzolan, or with fly ash or with calcined shales or with calcined pozzolans or with silica fume or PC mixed or pozzolanic cement or compound cement, for that such pathology problems will not occur when reinforced concretes nor mass concretes are used for building. Hence the very particular and special focus about this research, having confined only to the natural or artificial pozzolans, considering them all, regardless of their origin, approach as materials consisting of small crystalline fractions randomly distributed in a largely vitreous and/or amorphous matrix, which confers their reactivity with calcium hydroxide in the form of slaked lime or portlandite from PC. In turn, these vitreous and/or amorphous fractions consist in its greater part, by reactive silica, SiO2r-, reactive alumina, Al2O3r-, and reactive iron oxide, Fe2O3r-, which also receive, in conjunction, the specific name of "hydraulic factors" of the pozzolan. Usually, they all differs in quantity of their respective total contents of the SiO2 (%), Al2O3 (%) and Fe2O3 (%) determined the pozzolan by alkaline fusion by means of standard procedures. Of the three above-mentioned oxides reagents and from the technical point of view, the most important for its increased presence in every one of the natural or artificial pozzolans and also significance in the chemical durability that can get them to give the concrete mortar or cement paste which contain them, are SiO2r- and Al2O3r-. From the first two, the SiO2r- reacts with portlandite only, released in the hydration of the PC (and with Ca(OH)2), to form C-S-H gels, transformed in tobermorites or jennites later on, similar to C-S-H gels also originating from the alite and belite hydration in the CP. From the standardization criteria point of view, the presence of this silicic fraction in pozzolans is regulated at first, by the European standard EN 197-1, in general, also being referred by the EN 450-1:2006, in the context of the fly ash in addition to the concrete, as a "fine powder of spherical particles mainly crystalline form. It is from the combustion of pulverized coal, which have pozzolanic properties and is mainly composed of SiO2 and Al2O3". In addition and according to the EN 197-1 standard, the reactive silica content (which can be defined and determined in accordance with EN 197-1 standard or its UNE 80225 standard) must not be lower than 25 % of its mass. Meanwhile, considerable experimental studies by Talero and Talero et al, have shown that if the pozzolan is not adequate in quality nor quantity, the durability of cement that is part and, therefore, of its derivative products, concretes, mortars and pastes cement, can become abnormally low because its reactive alumina, Al2O3r- (%), content or tetra- or penta-coordinated alumina which involves itself in a very direct and totally mixed and conflicting results even at all aggressive chemical attack natural to produce to the concrete, mortar and paste with inadequate natural and/or artificial pozzolans, such as those from the selenitous land and waters (sulfates, strikes if the material itself concrete, mortar and paste that contain them, for rapid forming ettringite form, ett-rf, which can even cause rapid gypsum attack to said concrete). In contrast, sea spray and de-icing salts (chlorides strikes the reinforced steel concrete causing them electrochemical corrosion by "bite", although in that other chemical attack, such Al2O3r- causes rapid Friedel's salt formation, Fs-rf, too, to cause protector effect of the electrochemical corrosion of reinforcements for these chlorides), seawater (mutual aggressive action of chlorides and sulfates), carbonation, alkali-silica reaction, and, in addition, to influence the release of hydration heat, as well as in the rheological behavior of the pastes, etc., decreasing/shorting them thus their expected durability and sometimes even very seriously. But the most ironic thing is, that despite its referral totally opposed, compared to sulfates and chlorides, behaviour, - far not available is, a chemical analysis method for its quantitative determination, which is also relatively simple in operation, accurate, precise, rapid response, from a technical point of view for building (no more than 28 days), repeatable, reproducible, not involving danger to life safety of the people who need to manipulate and implement, economic, and also serve for both scientific research and technical side, and - has yet to be any chemical specification that sets maximum levels for Al2O3r-(%) in the natural or artificial pozzolan to be added to the cement and/or to the concrete that will be subject to a particularly aggressive chemical attack from those mentioned above, and in particular, to sulphates, chlorides or sea water. And for the sake of and justification of this need, it has to be said that the current Spanish Instruction for Structural Concrete EHE-08 does not provide any specification on "hydraulic factors" in general, nor the content of Al2O3r- (%) in fly ash, very particular, as Article 30º "Additions", or any other Article, although does provide, however, other chemical specifications lacking the necessary meaning in terms of the message Chemical Durability mentioned, nor the Article 37º which and for greater sake, is paradoxically entitled "Durability of the concrete and of their reinforcements". It has also not contemplated in the latest version just released from EN 197-1 standard entitled "Cement Part 1: Composition, specifications and conformity criteria for common cements". Nor, in EN 450-1:2006 entitled "Fly ash for concrete Part 1: Definitions, specifications and conformity criteria", nor by current Spanish Instruction for Cement Reception, RC-08, nor the ASTM C618-03 Standard either. The only chemical specification that both Standards, European and American, refer is one that says that the sum of the total contents of SiO2 (%), Al2O3 (%) and Fe2O3 (%) of natural and artificial pozzolan, must be greater than or equal to 70 % , defining pozzolans thus: "siliceous or aluminous and siliceous materials, which themselves do not have any cementitious value but finely divided and in the presence of moisture it reacts with calcium hydroxide at ordinary temperatures to form compounds possessing cementitious properties". Consequently and according to everything related before, the primary objective of this Doctoral Thesis has been: To design and start-up a new quantitative analytical method of technical utility (the maximum test duration is not more than 28 days), to determine the content of reactive alumina content, Al2O3r- (%), vitreous or amorphous alumina, of natural and artificial pozzolans. And once designed, validate at repeatability level and in accordance with appropriate statistical parameters with special emphasis on the acceptance criteria established by the American Association of Official Analytical Chemists (AOAC). And to achieve this, the innovation of this research has been based on the following general principles, namely: - All the alumina in any pozzolan, natural or artificial, that can be attacked, dissolved and leached by portlandite or calcium hydroxide, Ca(OH)2, in aqueous medium, is considered reactive alumina, Al2O3r-. - This aluminic fraction of natural or artificial pozzolan to analyze and study, has to be in such physical-chemical state that it can react in the presence of calcium hydroxide, sodium chloride and water status and to cause monochloro-aluminate hydrated calcium, C3A·CaCl2·10H2O or Friedel's salt. Moreover, such physical-chemical state of the pozzolan must be consistent with the definition of reactive alumina adopted in this research because of the actual performance of chemical durability that can reach confer on blended cements and their derivatives, concretes, mortars and pastes that are manufactured with the same. - The originality of this new analytical method, compared to the other methods for determining reactive alumina existing (collected in abbreviated form in the state of the art of this report), is the quantification of such aluminic fraction of natural or artificial pozzolans is performed by stoichiometric calculation based on this, in the chemical reaction of Friedel's salt formation after 28 days of the analysis of saline-basic hydration accelerated natural or artificial pozzolan also performed in this research, and the quantitative determination of the Friedel's salt has been performed by two instrumental analytical techniques known as thermogravimetric analysis (variant I), and Rietveld method with X-ray powder diffraction (variant II). - The chemical reaction of Friedel's salt formation after 28 days of accelerated saline-basic hydration of the selected natural and artificial pozzolan, was optimized to ensure that the single chemical compound of aluminium and chlorine formed was Friedel's salt only (dosing for this purpose in amount suitable chemical reagents: Ca(OH)2, NaCl and distilled water), and, on the other hand, maintaining the appropriate compromise between the highest yield from the chemical reaction (attack, dissolution and leaching in 28 days, all reactive alumina of pozzolan) and to accelerate the etching media, which were 40°C temperature, constant stirring and sealing the reactor. - The applicability and selectivity of the new analytical method, the subject of this Doctoral Thesis, was studied with a series of Spanish natural and artificial pozzolans, siliceous or siliceous and aluminous in nature, which were as follows: M0 (metakaolin 0), M1 (M0 with 50 % quartz), L and C (natural pozzolans of the Canary Islands), CV10 (fly ash 10), CV17 (fly ash 17), A (natural pozzolan of Almagro), O (natural pozzolan of Olot), and HS (silica fume). - All mineral admixtures were selected satisfied the physical and chemical requirements proposed to consider them as pozzolan, which was mandatory, so its Al2O3r- (%) content can determine by the new analytical method. These conditions were as follows: adequate degree of fineness of grind or average particle size (according to EN 451-2 standard), have been analyzed chemically (according to EN 196-2 or ASTM C311 standards), in order to determine their total contents of SiO2 (%), Al2O3 (%) and Fe2O3 (%), mainly, having also determined its reactive silica content, SiO2r- (%) (UNE 80225 standard), and fulfilled with testing of pozzolanicity or Frattini test (according to EN 196-5 standard) at 28 days age at least. The last criteria was mandatory until 1988, for any natural and artificial pozzolan to a factory intended to introduce cements in the manufacturing process of a new Portland cement type CEM IV pozzolanic additions, and with this Doctoral Thesis has made is to be used once again mandatory. And the results obtained using the new analytical method, of the Al2O3r- (%) content for each selected pozzolan, were as follows: - by its variant I: M0 29.9 % , M1 16.9 % , CV10 11.4 % , L 12.3 % , C 12.6 % , A 8.0 % , CV17 9.5 % and O 6.3 % of Al2O3r-, and - by its variant II: M0 30.7 % , M1 15.4 % , CV10 14.7% % , L 11.8 % , C 11.1 % , A 8.9 % , CV17 9.6 % and O 6.8 % of Al2O3r-. Finally, they would all be further contrasted by the calibration and validation of new analytical method, with reference values obtained from these same natural and artificial pozzolans, which had been given by the method of Florentin, consisting of attack, dissolve and leached with a basic attack (with Ca(OH)2 in aqueous medium and laboratory temperature, having also been its duration up to 1 year), followed by another acid attack (HCl, d = 1.12), all soluble aluminic fraction of pozzolan, and in particular their total content of reactive alumina, Al2O3r-(%), was this time as follows: M0 28.8 %, M1 16.7 %, CV10 9.7 %, L 11.2 %, C 12.2 %, A 13.0 %, CV17 10.6 % and O 9.5 % (and their siliceous and iron contents that are at par). This validation has shown on the new analytical method is more reliable than Florentin method, so it turns out to be entirely appropriate to get the results that have been tried by the same, besides providing them a relatively short space of time (28 days at most) and reasonably no high economic cost (unless mistake -free direct and indirect taxes, such economic cost would be between 800.00 - 900.00 €/pozzolan (most likely case), and about an one-third part around, in the event that the maximum age of the accelerated test is 7 days only (less likely case). So it is technically acceptable, to consider the range of possible variability of concentrations or contents pozzolan analyte with validation parameters such as: linearity (the results obtained are directly proportional to the instrumental response signal received), excellent sensitivity and accuracy, satisfactory repeatability values from the contents of each and Al2O3r- (%) each selected pozzolan, confirming therefore universal use. Finally, the advantages of the new analytical method over existing methods compiled from literature (Florentin method , the Lopez Ruiz method -HF and HNO3 40 % 2N-, the method of Murat and Driouche for clays -0.5M HF-, the method of Arjuan, Roy and Silbee for fly ash -HF 1 %- and its modification by Fernández-Jiménez et al -HF 1 %, 27Al MAS NMR and XRD (Rietveld method)-, and the method for determining the SiO2r-/Al2O3r- clay and fly ash ratio of Santaquiteria Ruiz et al -HF 1 %, NaOH 8M and ICP-AES-) are primarily and relatively short time get the result intended answer (28 days at most), its considerable lower cost, free from danger to the life safety of the people who need to manipulate and put in practice as well as its universality and selectivity, since it is applicable for all types of natural or artificial pozzolans, as it has been shown by the results of selected natural and artificial pozzolanic materials and analyzed in a range of analyte concentrations -reactive alumina, Al2O3r- (%) content- from 5 % to 30 % by mass, this range, on the other hand, includes virtually ALL existing transnational market in natural and artificial pozzolans and still exist. Therefore and in accordance with the above, the new analytical method is already performed by the variant I or II, because, - firstly, grounded to experimental approaches concerning its experimental procedure -"modus operandi"- which has been applied to a wide range of natural and artificial pozzolans, and - secondly, due to the quality of the results obtained with a great degree of accuracy and repeatability, it has been shown to possess significant scientific value in the research, but especially technical value -for quality control of natural and artificial pozzolans commonly added to Portland cement factory and/or directly to their concrete and mortar in plant-, and also be representative enough of the values obtained by the same probable chemical durability that each of them can reach out to give the concrete mortar and paste to which it belongs, as well as proper amount of partial replacement of Portland cement. To get in line with their own chemical-physical or physical-chemical features which can come to confer, as its chemical character (aluminic, silicic-aluminic, aluminic-silicic, aluminic-ferric-silicic or ferric-silicic), form and medium size of its particle is. Finally, the new analytical method has proven to meet all mandatory requirements established by ISO/IEC 17025 on the quality and reliability of new methods or analytical procedures are not standardized yet, in order to be considered appropriate this new analytical method, in this Doctoral Thesis it is to be proposed in the near future, before the corresponding AENOR (Spanish Association for Standardization and Certification) Commission, to start a procedure for certification and standardization.

The TRANSPLANTA collection of Arabidopsis lines: a resource for functional analysis of transcription factors based on their conditional overexpression.

Los factores de transcripción (FTs) son reguladores clave de la expresión génica en todos los organismos. En eucariotas los FTs con frecuencia están representados por miembros funcionalmente redundantes de familias génicas de gran tamaño. La sobreexpresión de FTs puede representar una herramienta para revelar las funciones biológicas de FTs redundantes en plantas; sin embargo, la sobreexpresión constitutiva de FTs con frecuencia conlleva diversos defectos en el desarrollo, impidiendo su caracterización funcional. Sin embargo, aproximaciones de sobreexpresión condicional podrían ayudar a solventar este problema. En el consorcio TRANSPLANTA, en el que participan varios laboratorios del CBGP, hemos generado una colección de líneas transgénicas de Arabidopsis, cada una de las cuales expresa un FT bajo el control de un promotor inducible por ?estradiol. Hasta el momento se han generado 1636 líneas homocigotas independientes que corresponden a 634 FTs diferentes, lo que representa una media de 2,6 líneas por cada FT. Como confirmación de la utilidad de esta herramienta, el tratamiento con ?estradiol de líneas que expresaban condicionalmente FTs provoca alteraciones fenotípicas tales como proliferación de pelos radiculares, senescencia inducida por oscuridad, acumulación de antocianinas y enanismo, y que corroboran fenotipos previamente descritos debidos a la sobreexpresión de dichos FTs. Rastreos realizados posteriormente con otras líneas TRANSPLANTA han permitido la identificación de FTs implicados en diferentes procesos biológicos de plantas, confirmando que la colección es una herramienta valiosa para la caracterización funcional de FTs. Las semillas de las líneas TRANSPLANTA han sido depositadas en el Nottingham Arabidopsis Stock Centre para su distribución posterior.

Methodology for assessing regional economic impacts of charging HGVs in Spain: an integrated approach through a random utility based multiregional input-output and a road transportation network model

An integrated approach composed of a random utility-based multiregional input-output model and a road transport network model was developed for evaluating the application of a fee to heavy-goods vehicles (HGVs) in Spain. For this purpose, a distance-based charge scenario (in euros per vehicle kilometer) for HGVs was evaluated for a selected motorway network in Spain. Although the aim of this charging policy was to increase the efficiency of transport, the approach strongly identified direct and indirect impacts on the regional economy. Estimates of the magnitude and extent of indirect effects on aggregated macroeconomic indicators (employment and gross domestic product) are provided. The macroeconomic effects of the charging policy were found to be positive for some regions and negative for other regions.