El sistema de producción localizado de leche y derivados lácteos de Santa Cruz Aquiahuac, Tlaxcala, México

Se describe el sistema de producción localizado (SPL) de producción de leche y derivados lácteos de Aquiahuac, una pequeña comunidad ubicada en el estado de Tlaxcala, México. Este territorio desde la época prehispánica ha sufrido una fuerte presión sobre sus recursos naturales y actualmente presenta el mayor nivel de industrialización del Estado.   Se considera que el conocimiento constituye el elemento más importante de la producción y si éste llega a convertirse en patrimonio compartido por la comunidad local debe ser potencializado en beneficio de los pobladores rurales.   Se revisa la bibliografía relacionada con el desarrollo local y el territorio; se esbozan antecedentes históricos de la ganadería lechera en la comunidad y finalmente se reseña la manera en que se desarrollo la cadena productiva de leche y de queso en Aquiahuac.   ABSTRACT This article describes the local agro-food systems of milk and dairy products in Aquiahuac, a small community located in the state of Tlaxcala in México. This territory has suffered, since prehispanic era, a strong pressure upon its natural resources. Nowadays, it presents the major industrialization level in the state.   It is considered that knowledge constitutes the most important element in production, and if it becomes a share wealth for the local community, it must be potentially used in benefit for rural inhabitant.   This is a literature review related to territory and local development, it gives details of the way that dairy and cheese chain production develops in Aquiahuac.

Innovación tecnológica, oportunismo y acción colectiva en la concentración quesera de Tlaxco, Tlaxcala, México

Tlaxco es una pequeña cuenca lechera del centro de México. Concentra a los diferentes actores implicados en la cadena productiva de queso: ganaderos, recolectores de leche, queseros y comerciantes. Se estudió a través de la metodología de los Sistemas Agroalimentarios Localizados (SIAL) que permite un análisis multidisciplinario. La actividad quesera se desarrolló por la disponibilidad de leche, y los comerciantes han aprovechado la fama que ha ganado el queso a nivel regional. Los agentes se caracterizan por la pequeña escala en sus actividades y un bajo nivel tecnológico y económico; a pesar de eso, la quesería sigue desarrollándose, pero las relaciones son limitadas: las definen la falta de confianza, oportunismo e individualismo. El lugar de promover colectivamente la calidad, la competencia se basa exclusivamente en el precio. La falta de activación colectiva de la cuenca debilita a los actores e impide un uso óptimo de los recursos.   ABSTRACT Tlaxco, is a small dairy area ubicated in the central part of México. The different actors implicated in cheese value chain are concentrated in this zone: dairy farmers, milk collectors, cheese makers and sellers. The Local Agrifood System methodology has been applied to bring us a multidisciplinary point of view. Milk availability made possible cheese-making activity, and Tlaxco cheese enjoy a regional reputation. Actors are characterized by small scale activities and a low technological and economical level. Nevertheless. Cheese-making activities are still in development. But relationships are limited: their main characteristics are mistrust. Opportunism and individualism. Instead of promoting collectively product quality, competition is almost only based on price. The lack of collective activation in the area weakens actors and stops an optimal use of resources.

Las rutas alimentarias, herramienta para valorizar productos de las agroindustrias rurales. El caso de la ruta del queso Turrialba, Costa Rica

En la búsqueda de estrategias para el desarrollo de las zonas rurales, aparece la actividad del turismo como un instrumento de aplicación práctica que puede contribuir a generar procesos de desarrollo local que permitan complementar los ingresos agrícolas, crear nuevos puestos de empleo y evitar el despoblamiento rural.   El agroturismo y las rutas alimentarias entendidas como modalidades del turismo rural resultan de especial interés porque representan nuevas actividades en el medio rural que agregan valor a las producciones agroalimentarias en manos de pequeños productores y en especial a aquellas que emplean procesos artesanales de elaboración.   En el actual contexto de apertura comercial gobernada por los commoditties, el fast-food y las barreras sanitarias, la existencia de muchas pequeñas agroindustrias rurales se ve amenazada puesto que no alcanzan los niveles de productividad para competir en mercados globalizados. Muchas de esas empresas podrían tener una salida si logran articularse con el negocio del turismo rural, por medio de la prestación de productos y servicios para atender las demandas de los turistas interesados en conocer aspectos de la cultura agropecuaria y agroindustrial de una determinada región. Esta propuesta nos lleva a analizar las características generales del turismo rural, el agroturismo y las rutas alimentarias, así como la manera en que esos conceptos pueden aplicarse en un territorio específico, para potenciar procesos y productos con identidad, como es el caso del queso Turrialba en Costa Rica, alrededor del cual se propone la construcción de una ruta alimentaria como estrategia de desarrollo local y valorización de la agroindustria quesera artesanal.   ABSTRACT This paper takes us to analyze the general characteristics of rural tourism, agro-tourism and food routes and of how those concepts may be applied in specific territories, to promote processes and develop products with territories, to promote processes and develop products with territorial identity, as is the case of the Turrialba cheese in Costa Rica. Tourism appears to be an instrument of practical application that can contribute to generate processes of local development that helps to complement agricultural incomes, create new employments and avoid rural migration. Agro-tourism and food routes are of special interest since they represent new activities in the rural areas which add value to the agro-alimentary productions through the hands of small producers.

Potencialidades de desarrollo y movilización local: un estudio SIAL en Santa Cruz de Turrialba

El queso fresco blanco, conocido como queso Turrialba en Costa Rica, es un producto de mucho valor económico  y social, que ha despertado un interés particular en el análisis de sus estructuras de producción y las redes interrelaciónales entre los diferentes actores involucrados en su elaboración y comercio local y regional.  Se comprobó la existencia de un SIAL en Santa Cruz de Turrialba que cuenta con organizaciones con objetivos y metas comunes que han dado origen a acciones colectivas operativas. Este SIAL ha generado también acciones operativas individuales que han diversificado, entre otras, la comercialización de su producto. En el plano colectivo, la planificación y priorización de sus proyecciones futuras están afectando no solamente el área de la producción agroindustrial sino también otros campos como el turismo agroecológico.   ABSTRACT The fresh White cheese in Costa Rica is well known as “Turrialba cheese”. This cheese has high economic and social value and its production structure and its local and regional relations networks became a very interesting fact of analysis. It has been proved the occurrence of a local Agrofood System (SIAL) in Santa Cruz of Turrialba, where organizations with specific common purposes and goals were present. This situation originated individual operative action  as in order to diversity their products and markets. On the collective side, the planification and priorization of their future projects and ideas, have influenced not only the production, but also another fields like the development of agro ecological tourism in the region.

Designing a healthy food partnership: lessons from the Australian food and health dialogue

BACKGROUND: Poor diets are a leading cause of disease burden worldwide. In Australia, the Federal Government established the Food and Health Dialogue (the Dialogue) in 2009 to address this issue, primarily through food reformulation. We evaluated the Dialogue's performance over its 6 years of operation and used these findings to develop recommendations for the success of the new Healthy Food Partnership.

METHODS: We used information from the Dialogue website, media releases, communiqués, e-newsletters, materials released under freedom-of-information, and Parliamentary Hansard to evaluate the Dialogue's achievements from October 2013 to November 2015, using the RE-AIM (reach, efficacy, adoption, implementation and maintenance) framework. We also engaged closely with two former Dialogue members. Our findings update a prior assessment done in October 2013.

RESULTS: Little data is available to evaluate the Dialogue's recent achievements, with no information about progress against milestones released since October 2013. In the last 2 years, only one additional set of sodium reduction targets (cheese) was agreed and Quick Service Restaurant foods were added as an area for action. Some activity was identified in 12 of a possible 137 (9 %) areas of action within the Dialogue's mandate. Independent evaluation found targets were partially achieved in some food categories, with substantial variation in success between companies. No effects on the knowledge, behaviours or nutrient intake of the Australian population or evidence of impact on diet-related disease could be identified.

CONCLUSIONS: The new Healthy Food Partnership has similar goals to the Dialogue. While highly laudable and recognised globally as cost-effective, the mechanism for delivery in Australia has been woefully inadequate. Strong government leadership, adequate funding, clear targets and timelines, management of conflict of interest, comprehensive monitoring and evaluation, and a plan for responsive regulation in the event of missed milestones will be required if the new Healthy Food Partnership is to achieve its urgent public health goals.

Estudo de composto com propriedades funcionais em flor de cardo submetida a diferentes tratamentos de secagem

O cardo é uma planta que encontra-se associada ao fabrico de queijo, pois é responsável pelo fenómeno da coagulação do leite. No entanto, têm aumentado o número de estudos realizados nesta planta, relacionados com a composição química, de forma a perceber os benefícios para a saúde. O objetivo desta dissertação foi caracterizar a flor de cardo relativamente à sua atividade antioxidante, composição em compostos fenólicos e avaliar a sua estabilidade ao longo do trato digestivo. Para tal, foram estudas amostras de flor de cardo da espécie C. cardunculus spp flavescens, após liofilização e secagem em estufa com convecção forçada a diferentes temperaturas (40ºC, 50ºC e 60ºC). Após cada tratamento, efetuaram-se duas extrações sucessivas com soluções de metanol (98% v/v) e de acetona (60% v/v). Os extratos obtidos foram depois utilizados para quantificar os teores em compostos fenólicos totais, em orto-difenois e em flavonóides. A atividade antioxidante foi determinada utilizando os métodos DPPH e ABTS. Por fim, procedeu-se à avaliação da bioacessibilidade dos compostos presentes submetendo os vários extratos a condições simulantes do trato digestivo. Pela análise dos resultados obtidos foi possível constatar que a quantidade de compostos fenólicos e atividade antioxidante na flor de cardo varia consoante a temperatura de secagem, ocorrendo a diminuição à medida que se aumenta a temperatura. Os resultados obtidos para a bioacessibilidade mostraram um comportamento distinto dos extratos de metanol e de acetona. No entanto, em ambos os casos ocorreu uma diminuição na quantidade de compostos disponíveis para absorção intestinal.

Grape and Wine Metabolites: Biotechnological Approaches to Improve Wine Quality

Grape metabolites can be affected by many extrinsic and intrinsic factors, such as grape variety, ripening stage, growing regions, vineyard management practices, and edaphoclimatic conditions. However, there is still much about the in vivo formation of grape metabolites that need to be investigated. The winemaking process also can create distinct wines. Nowadays, wine fermentations are driven mostly by single-strain inoculations, allowing greater control of fermentation. Pure cultures of selected yeast strains, mostly Saccharomyces cerevisiae, are added to grape must, leading to more predictable outcomes and decreasing the risk of spoilage. Besides yeasts, lactic acid bacteria also play an important role, in the final wine quality. Thus, this chapter attempts to present an overview of grape berry physiology and metabolome to provide a deep understanding of the primary and secondary metabolites accumulated in the grape berries and their potential impact in wine quality. In addition, biotechnological approaches for wine quality practiced during wine alcoholic and malolactic fermentation will also be discussed.

From field to fermentation: characterisation and application of non-dairy cultures in dairy foods

This thesis investigates the phenotypic and genotypic diversity of non-dairy L. lactis strains and their application to dairy fermentations. A bank of non-dairy lactococci were isolated from grass, vegetables and the bovine rumen. Subsequent analysis of these L. lactis strains revealed seven strains to possess cremoris genotypes which did not correlate with their observed phenotypes. Multi-locus sequence typing (MLST) and average nucleotide identity (ANI) highlighted the genetic diversity of lactis and cremoris subspecies. The application of these non-dairy lactococci to cheese production was also assessed. In milk, non-dairy strains formed diverse volatile profiles and selected strains were used as adjuncts in a mini Gouda-type cheese system. Sensory analysis showed non-dairy strains to be strongly associated with the development of off-flavours and bitterness. However, microfluidisation appeared to reduce bitterness. A novel bacteriophage, ɸL47, was isolated using the grass isolate L. lactis ssp. cremoris DPC6860 as a host. The phage, a member of the Siphoviridae, possessed a long tail fiber, previously unseen in dairy lactococcal phages. Genome sequencing revealed ɸL47 to be the largest sequenced lactococcal phage to date and owing to the high % similarity with ɸ949, a second member of the 949 group. Finally, to identify and characterise specific genes which may be important in niche adaptation and for applications to dairy fermentations, comparative genome sequence analysis was performed on L. lactis from corn (DPC6853), the bovine rumen (DPC6853) and grass (DPC6860). This study highlights the contribution of niche specialisation to the intra-species diversity of L. lactis and the adaptation of this organism to different environments. In summary this thesis describes the genetic diversity of L. lactis strains from outside the dairy environment and their potential application in dairy fermentations.

Processamento de imagem digital para o controlo de qualidade do queijo regional de Évora

A utilização generalizada do computador para a automatização das mais diversas tarefas, tem conduzido ao desenvolvimento de aplicações que possibilitam a realização de actividades que até então poderiam não só ser demoradas, como estar sujeitas a erros inerentes à actividade humana. A investigação desenvolvida no âmbito desta tese, tem como objectivo o desenvolvimento de um software e algoritmos que permitam a avaliação e classificação de queijos produzidos na região de Évora, através do processamento de imagens digitais. No decurso desta investigação, foram desenvolvidos algoritmos e metodologias que permitem a identificação dos olhos e dimensões do queijo, a presença de textura na parte exterior do queijo, assim como características relativas à cor do mesmo, permitindo que com base nestes parâmetros possa ser efectuada uma classificação e avaliação do queijo. A aplicação de software, resultou num produto de simples utilização. As fotografias devem respeitar algumas regras simples, sobre as quais se efectuará o processamento e classificação do queijo. ABSTRACT: The widespread use of computers for the automation of repetitive tasks, has resulted in developing applications that allow a range of activities, that until now could not only be time consuming and also subject to errors inherent to human activity, to be performed without or with little human intervention. The research carried out within this thesis, aims to develop a software application and algorithms that enable the assessment and classification of cheeses produced in the region of Évora, by digital images processing. Throughout this research, algorithms and methodologies have been developed that allow the identification of the cheese eyes, the dimensions of the cheese, the presence of texture on the outside of cheese, as well as an analysis of the color, so that, based on these parameters, a classification and evaluation of the cheese can be conducted. The developed software application, is product simple to use, requiring no special computer knowledge. Requires only the acquisition of the photographs following a simple set of rules, based on which it will do the processing and classification of cheese.

Evaluation of the evolution of the anthocyanins profile in red wine grape varieties in Alentejo

The flavonoids (including anthocyanins) are wine compounds with important anti-oxidant activity, protecting the cells against oxidative processes, preventing cardiovascular and neurodegenerative diseases, cancer, among others (Antoniolli et al. 2015; Castañeda-Ovando et al. 2009; Hosu et al. 2014; Huang et al. 2009; Kong et al. 2003). Anthocyanins in grapes at harvest are determinant to red wine quality and their development in the grape must be characterised in order to determine the most suitable date for the harvest. Thus the aim of this research is the evaluation of anthocyanins composition in two red wine grape varieties from véraison continuing through ripening. Anthocyanins were quantified by high resolution liquid chromatography (HPLC-DAD). Additionally, the total phenols content were quantified by UV-Vis Spectrometry. The anthocyanins’ profile evolution may be dependent on the variety and ripening phase. During ripening grape samples have shown an increase of coumaryl derivatives. This information may lead us to understand the anthocyanins biosynthesis pathway in different grape varieties. The development of anthocyanins from the véraison seems to follow a pattern that coincides with the increasing accumulation of soluble sugars.

Effect of autochthonous starter cultures in the production of "Paio", a traditional Portuguese dry- cured sausage

Traditional dry-cured sausages are highly appreciated in Mediterranean countries. The aim of the present study was to evaluate the effect of different starter cultures in the sausages Alentejano pig meat was used to prepare drycured sausages in a local factory. Staphylococcus xylosus, Lactobacillus sakei and a yeast strain were inoculated at a concentration of 106 cfu/g meat batter both in separate and in mixed culture. Three independent batches with two replicates per treatment were produced. Samples were collected throughout the ripening process. pH and aw were determined according to the ISO standards. Microbiological counts of total mesophiles, total psycrotrophs, anaerobes, coagulase-negative staphylococci (CNS), lactic acid bacteria (LAB), enterobacteria, yeasts and moulds and Listeria monocytogenes were done according to the respective ISO standards, as well as detection of Salmonella spp. Biogenic amines quantification was performed by HPLC as described by Roseiro et al. (1). The treatment with L. sakei alone was the most effective in reducing the contamination level both with Salmonella spp. and L. monocytogenes, however this effect seems to be lost in the mixed cultures. The presence of the yeast strain seems to increase the levels of phenylethylamine and histamine. The contents in cadaverine, putrescine and tyramine were generally lower in the inoculated sausages. Regarding tyramine, the treatments with L. sakei showed significantly lower values. No significant differences between treatments were observed for both spermine and spermidine.

The women role in the agroenvironmental activities of Amazon agroforeststry systems.

This paper presents the results of a research on the way which shows the role of the farmer women to contribute to overcome the life hindrances in the Amazonian agroforest environment, for food safety and familiar income. The sample was the women of the Associação de Produtores Alternativos - APA, Ouro Preto do Oeste, Rondônia, Brazil. Questionnaires were applied to 50 women of this community, in 2004/2005, with additional interviews in 2006, taking into account their role in the farms, and their several invisible activities. The role of APA?s women was compared to that of other Amazonian women, like rubber tappers and riverine people. The APA?s women perform tasks as field manpower - 78.0% of them, making decisions about what to plant - 18.0%, harvest destination - 32.0%, which animals to raise -14.0% and animal products destination -34.0%. Traditionally, in rural zone, the women with their children play a role as non-paid manpower, getting some monetary income with occasional commercialization of on-farm processed products as cheese, pickles, jellies and fruit liqueurs. They are responsible for the cultures practiced around the house. All these Amazonian women edaphoclimatic conditions, greatly contributing to the biodiversity, conservation, and to ecological, social and economic stability.are guardians, perpetuators and disseminators of a rich germplasm adapted to local.

Susceptibility and Interactions of Drosophila suzukii and Zaprionus indianus (Diptera: Drosophilidae) in Damaging Strawberry.

Resumo: Drosophila suzukii (Matsumura) foi recentemente detectado causando danos idade para morangos no Brasil. Infestação na cultura de morango tem frequentemente foi observado conjuntamente com a presença de Zaprionus indianus Gupta. este estudo investigou a suscetibilidade de morangos em três amadurecimento estágios para infestação de D. suzukii e Z. indianus e sua interação. Abstracts: Drosophila suzukii (Matsumura) has been recently detected causing damage to strawberries in Brazil. Infestation in strawberry culture has often been observed jointly with the presence of Zaprionus indianus Gupta. This study investigated the susceptibility of strawberries at three ripening stages to infestation of D. suzukii and Z. indianus and their interaction. In the laboratory, strawberries cv. Albion at different ripening stages (green, semi-ripe and ripe) were exposed to D. suzukii and Z. indianus for 24 h in choice and no-choice bioassays. Additionally, we evaluated the effects of mechanical damage incurred artificially or by D. suzukii ovi-position on Z. indianus infestation. In no-choice bioassay, there were no significant differences in fruit susceptibility to D. suzukii infestation at different ripening stages. However, in choice bioassay, D. suzukii adults preferred to oviposit on R fruit. The presence of mechanical damage did not increase susceptibility of fruit to D. suzukii oviposition. For Z. indianus , there was greater susceptibility of R fruit in relation to SR and G fruit in both the choice and no-choice bioassays. There was a significant and positive interaction of mechanical damage and damage caused by D. suzukii to R fruit and infestation by Z. indianus , which was not observed in SR and G fruit. Although infestation of Z. indianus is related to attack damaged or decaying fruit, this work shows that this species has the ability to oviposit and develop in healthy strawberry fruit with and increased infestation level when the fruit has damage to its epidermis.

Régime thermique en période de maturation du raisin dans le géoclimat viticole.

Mots clés :indice de fraîcheur des nuits, amplitude thermique, Système CCM Géoviticole, zonage, qualité. Key words: cool night index, thermal amplitude, MCC System for World Viticulture, zoning, quality. RESUME Le régime thermique en période de maturation du raisin est l?une des variables déterminantes de la coloration du raisin et de la richesse en arômes, anthocyanes et polyphénols des vins. L?objectif du travail est de caractériser le régime thermique, notamment la fraîcheur des nuits et l?amplitude thermique au cours de la maturation, au niveau du climat viticole mondial, sur une base des données de 100 régions viticoles dans 30 pays, obtenue par l?intermédiaire de l?Organisation Mondiale de la Météorologie - OMM. Plusieurs indices climatiques viticoles ont été calculés: l?Indice de Fraîcheur des Nuits ?IF (ºC), l?Indice Héliothermique de Huglin ?IH (ºC) et l?Indice de Sécheresse ?IS (mm) du Système de Classification Climatique Multicritères Géoviticole, et l?amplitude thermique moyenne en août et septembre Aa-s (ºC). Egalement, sur la période véraison-récolte ?v-r(moyenne des 30 jours précédant la date de récolte, estimée sur la base d?un Indice Héliothermique de HUGLIN égal à 1.900 - approximatif pour la maturation du Cabernet-Sauvignon): la fraîcheur des nuits (FNv -r), la température moyenne de l?air (Tv-r), la température maximale de l?air (Txv-r) et l?amplitude thermique (Av-r). Les résultats montrent que IH est corrélé avec Tv-r (r=0,79**) et avec Txv-r (r=0,80**). IH représente donc bien les conditions thermiques générales de la période de maturation en ce qui concerne la température moyenne et maximale de l?air. Mais IH n?est pas corrélé ni avec Aa-s ni avec Av-r. Par contre, IF est corrélé avec Aa-s (r=-0,70**) et FNv-r est corrélé avec Av-r (r=-0,69**). Cette corrélation doit justifier, en partie, l?usage assez courant de l?amplitude thermique comme indicateur de bonnes conditions thermiques de maturation pour les régions qui présentent des valeurs élevées. Mais ce raisonnement peut amener à des caractérisations erronées. Le travail met en évidence, également, ?importance de considérer le bilan hydrique des régions (IS) dans l?analyse du régime thermique sur la qualité du raisin. On peut onclure que pour avoir une bonne caractérisation du régime thermique en période de maturation il faut considérer la fraîcheur des nuits (IF étant un bon indicateur de FNv-r moyen des régions, avec un r=0,80**), caractérisation qui peut être améliorée avec l?information des températures maximales et de sur 11l?amplitude thermique en période de maturation du raisin. Les éléments présentés peuvent servir à améliorer les indices climatiques pour estimer le potentiel qualitatif du raisin des différentes régions viticoles, notamment en complément de IF. ABSTRACT The thermal conditions during the grape ripening period are important variables related to colour of the grapes, anthocyanins, polyphenols and flavour of the wine. The main purpose of this work was to characterise the thermal conditions, especially the night coolness and the thermal amplitude during maturation, in the geoclimate of the world vine culture. A database of 100 grape-growing regions of 30 countries obtained from the World Meteorology Organisation (WMO) was used. Some climatic indexes were calculated: Cool Night Index ?IF (°C), Huglin?s Heliothermal Index ?IH (°C) and Dryness Index ?IS (mm), from the Multicriteria Climatic Classification System for World Viticulture, and the thermal amplitude in August and September Aa-s (°C). Over véraison-harvest period ?v-r(mean of the 30 days before harvesting date, estimated on the basis of HUGLIN Heliothermal Index equal to 1,900 ? approximately value to ripen Cabernet-Sauvignon) similar indexes were obtained: the cool night (FNv-r), the mean air temperature (Tv-r), the maximal air temperature (Txv-r) and the thermal amplitude (Av-r). The results showed that IH is positively correlated with Tv-r (r=0.79**), Txv-r (r=0.80)and IF (r=0.67**). Therefore, IH represents well the general thermal conditions during maturation period, specially concerning the mean and the maximal air temperature. owever, IH was correlated neither with Aa-s nor to Av-r. IF was negatively correlated with Aa-s (r= -0.70**) and FNv-r was negatively correlated with Av-r (r=-0.69**). The correlation to some extent explains the current use of the thermal amplitude to predict good ripening thermal conditions for those regions that show high values. As here we have described, this thinking may give incorrect results. This work has also showed the importance to consider the water balance of the regions (IS) in the effect of the thermal conditions in grape quality. We conclude that the characterisation of the thermal conditions during the ripening period do need the cool night index (in this case, IFis a good index to provide the mean FNv-r of the regions, r=0,80**). Factors other than cool night which influence this characterisation are both maximal air temperature and thermal amplitude data. The elements presented in this work, in addition to IF , may improve the climatic indexes to be used to predict the qualitative potential of grapes from different regions.

Identificação de Staphylococcus spp. em amostras de queijos elaborados com leite de cabra.

2015