Synergistic Action of Actinoporin Isoforms from the Same Sea Anemone Species Assembled into Functionally Active Heteropores

Among the toxic polypeptides secreted in the venom of sea anemones, actinoporins are the pore-forming toxins whose toxic activity relies on the formation of oligomeric pores within biological membranes. Intriguingly, actinoporins appear as multigene families that give rise to many protein isoforms in the same individual displaying high sequence identities but large functional differences. However, the evolutionary advantage of producing such similar isotoxins is not fully understood. Here,using sticholysins I and II (StnI and StnII) from the sea anemone Stichodactyla helianthus, it is shown that actinoporin isoforms can potentiate each other’s activity. Through hemolysis and calcein releasing assays, it is revealed that mixtures of StnI and StnII are more lytic than equivalent preparations of the corresponding isolated isoforms. It is then proposed that this synergy is due to the assembly of heteropores because (i) StnI and StnII can be chemically cross-linked at the membrane and (ii) the affinity of sticholysin mixtures for the membrane is increased with respect to any of them acting in isolation, as revealed by isothermal titration calorimetry experiments. These results help us understand the multigene nature of actinoporins and may be extended to other families of toxins that require oligomerization to exert toxicity.

The Mishongnovi ceremonies of the Snake and Antelope fraternities / by George A. Dorsey -- and H. R. Voth.

v.3:no.3(1902)

The Oraibi summer snake ceremony, by H. R. Voth -- The Stanley McCormick Hopi expedition -- George A. Dorsey --

v.3:no.4(1903)

The truth about snake stories / by Karl P. Schmidt, Asst. Curator of Reptiles and Amphibians.

no.10(1929)

Stratigraphy of the unsaturated zone and uppermost part of the Snake River Plain aquifer at Test Area North, Idaho National Engineering Laboratory, Idaho /

Mode of access: Internet.

Report on Snake River streamflow depletion.

"Supplement A to Report ... prepared by a special task force of the Water Management Subcommittee, CBIAC, December, 1952" (7 l.) inserted at front of main report.

Upper Snake River Basin: Wyoming, Idaho, Utah, Nevada, Oregon. Public hearings. Boise, Idaho, U. S. Dept. of the Interior, Bureau of Reclamation, Region 1.

Hearings held at Idaho Falls, Idaho, Dec. 7, 1960; Twin Falls, Idaho, Dec. 8, 1960; Caldwell, Idaho, Dec. 9, 1960.

Upper Snake River Basin: Wyoming, Idaho, Utah, Nevada, Oregon. Boise, Idaho, U.S. Dept. of the Interior, Bureau of Reclamation, Region 1.

Mode of access: Internet.

The snake-dance of the Moquis of Arizona; being a narrative of a journey from Santa Fé, New Mexico, to the villages of the Moqui Indians of Arizona, with a description of the manners and customs of this peculiar people, and especially of the revolting religious rite, the snake-dance; to which is added a brief dissertation upon serpent-worship in general, with and account of the tablet dance of the pueblo of Santo Domingo, New Mexico, etc.

Mode of access: Internet.

Pollution effects on adult steelhead migration in the Snake River,

Prepared for Office of Research and Development, U.S. Environmental Protection Agency under project 18050DMB, program element 1B1021.

Traveltime, unit-concentration, longitudinal-dispersion, and reaeration characteristics of upstream reaches of the Yampa and Little Snake Rivers, Colorado and Wyoming /

Mode of access: Internet.