Environment monitoring technical report for the SON cage fish culture site at Bugungu, Napoleon Gulf, northern Lake Victoria, November 2013

Source of the Nile Fish farm (SON) is located at Bugungu area in Napoleon Gulf, northern Lake Victoria. The proprietors of the farm and the National Fisheries Resources Research Institute (NaFIRRI) have an established collaborative arrangement where NaFIRRI provides technical back‐stopping to enable quarterly environment monitoring of the cage site as a mandatory requirement of the National Environment Management Authority (NEMA). The agreed study areas are selected physical‐chemical factors (water depth, water transparency/secchi depth, water temperature, dissolved oxygen, pH, conductivity, and nutrient status), algal community (including primary production), aquatic invertebrates (zooplankton and macro‐benthos) and the fish community. This report presents field observations made during the fourth quarter (October‐December) field survey undertaken during December 2013; along with scientific interpretation and discussion of the results in reference to possible impacts of the cage facility to the water environment quality and aquatic biota.

Environment monitoring technical report for the SON cage fish culture site at Bugungu, Napoleon Gulf, northern Lake Victoria, June 2013

Source of the Nile Fish farm (SON) is located at Bugungu area in Napoleon Gulf, northern Lake Victoria. The proprietors of the farm requested NaFIRRI to provide technical assistance to undertake regular environment monitoring of the cage site as a mandatory requirement under the NEMA conditions. NAFIRRI agreed to undertake regular environment surveys in the cage area covering selected physical‐chemical factors (water column depth, water transparency, water column temperature, dissolved oxygen, pH, conductivity, nutrient status), algal aquatic invertebrates (micro‐invertebrates/zooplankton and macro‐benthos) and fish communities. During the year 2013, it was agreed with management to undertake quarterly environment monitoring surveys. However, the first quarter (January‐March 2013) survey was missed out due to late decision. The present report therefore covers the survey taken during the second quarter (April‐June 2013). Results/observations made are presented in this technical report along with a scientific interpretation and discussion of the results with reference to possible impacts of the cage facilities to the water environment and aquatic biota.

Bi-annual environment monitoring technical report for the SON cage fish culture site at Bugungu, Napoleon Gulf, northern Lake Victoria, December 2012

The first year-round quarterly surveys were completed for the year 2011. For the year 2012, SON management decided to change the frequency of the surveys from quarterly to biannual and the first such survey, was undertaken in June 2012. The second survey was undertaken in December 2012 and is the subject of this report: Results/observations made are presented in this technical report along with a scientific interpretation and discussion of the results with reference to possible impacts of the cage facilities to the water environment and aquatic biota. SON cage study sites were coded as downstream of cages (DSC), within cages (WIC) and upstream of cages (USC). Physical-chemical parameters (water column temperature, dissolved oxygen, pH, conductivity, were measured in-situ with a pre-calibrated hydrolab at each site. A digital Echo Sounder was used to determine the total water column depth at each site. A black and white Secchi disc was used to determine water column transparency. Coordinate locations were determined with a GPS device.

Environment monitoring technical report for the SON cage fish culture site at Bugungu, Napoleon Gulf, northern Lake Victoria, August 2014

Source of the Nile Fish farm (SON) is located at Bugungu area in Napoleon Gulf, northern Lake Victoria. The proprietors of the farm and the National Fisheries Resources Research Institute (NaFIRRI) have an established collaborative arrangement where NaFIRRI provides technical back-stopping to enable quarterly environment monitoring of the cage site; a mandatory requirement of the National Environment Management Authority (NEMA). The agreed study areas are physical-chemical factors (water depth, water transparency/secchi depth, water temperature, dissolved oxygen, BOO, pH, conductivity), and selected nutrients), algal community (including primary production), aquatic invertebrates (zooplankton and macrobenthos) and the fish community. This report presents field observations made during the third quarter (July-September) field survey undertaken during August 2014; along with scientific interpretation and discussion of the results in reference to possible impacts of the cage facility to the water environment quality and aquatic biota.

Environment monitoring technical report for the SON cage fish culture site at Bugungu, Napoleon Gulf, northern Lake Victoria, September 2013

Source of the Nile Fish farm (SON) is located in northern Lake Victoria close to the headwaters of the River Nile. The proprietors of the farm have established a collaborative agreement with the National Fisheries Resources Research Institute (NaFIRRI) to undertake quarterly environment monitoring surveys of the fish cage site at Bugungu in the Napoleon Gulf. This activity is a mandatory requirement of the National Environment Management Authority (NEMA) of Uganda. Therefore NAFIRRI undertakes monitoring surveys once every quarter covering selected physical‐chemical parameters including water column depth, water transparency, water column temperature, dissolved oxygen, pH, conductivity and nutrient status; algal, zooplankton, macro‐benthos and fish communities. While the first quarter survey of 2013 (January‐March) was missed out due to late decision, the second quarter monitoring survey was dully undertaken in May 2013 and a technical report was compiled and submitted to the client. The present report covers the third quarter survey (July‐September) undertaken in September 2013. Results/observations made are presented in this technical report along with a scientific interpretation and discussion of the results with reference to possible impacts of the cage facilities to the water environment quality and selected aquatic biota.

SON bi‐annual environment monitoring technical report for the cage culture site at Bugungu, northern Lake Victoria, June 2012

Source of the Nile Fish farm (SON) is located at Bugungu area in Napoleon Gulf, northern Lake Victoria. The proprietors of the farm requested NaFIRRI to provide technical assistance to undertake regular environment monitoring of the cage site as is mandatory under the NEMA conditions. NAFIRRI agreed to undertake regular environment surveys in the cage area covering selected physical‐chemical factors i.e. water column depth, water transparency, water column temperature, dissolved oxygen, pH, conductivity, redox potential and turbidity; nutrient status, algal and invertebrate communities (micro‐invertebrates/zooplankton and macro‐invertebrates/macro‐benthos) as well as fish community. The first year‐round quarterly surveys were completed for the year 2011. It was decided by SON management to change the frequency of the monitoring surveys to biannual starting in the year 2012 and the first such survey, which is the subject of this report, was undertaken in June 2012. Results/observations made are presented in this technical report along with a scientific interpretation and discussion of the results with reference to possible impacts of the cage facilities to the water environment and aquatic biota. SON

Environment monitoring technical report for the SON cage fish culture site at Bugungu, Napoleon Gulf, northern Lake Victoria, March 2014

Source of the Nile Fish farm (SON) is located at Bugungu area in Napoleon Gulf, northern Lake Victoria. The proprietors of the farm and the National Fisheries Resources Research Institute (NaFIRRI) have an established collaborative arrangement where NaFIRRI provides technical back‐stopping to enable quarterly environment monitoring of the cage site as a mandatory requirement of the National Environment Management Authority (NEMA). The agreed study areas are selected physical‐chemical factors (water depth, water transparency/secchi depth, water temperature, dissolved oxygen, pH, conductivity, and nutrient status), algal community (including primary production), aquatic invertebrates (zooplankton and macro‐benthos) and the fish community. This report presents field observations made during the first quarter (January‐March) field survey undertaken during March 2014; along with scientific interpretation and discussion of the results in reference to possible impacts of the cage facility to the water environment quality and aquatic biota. The

Trimeresurus stejnegeri snake venom plasminogen activator - Site-directed mutagenesis and molecular modeling

The specific plasminogen activator from Trimeresurus stejnegeri venom (TSV-PA) is a serine proteinase presenting 23% sequence identity with the proteinase domain of tissue type plasminogen activator, and 63% with batroxobin, a fibrinogen clotting enzyme from Bothrops atrox venom that does not activate plasminogen. TSV-PA contains six disulfide bonds and has been successfully overexpressed in Escherichia coli (Zhang, Y., Wisner, A., Xiong, Y. L,, and Bon, C, (1995) J. Biol. Chem. 270, 10246-10255), To identify the functional domains of TSV-PA, we focused on three short peptide fragments of TSV-PA showing important sequence differences with batroxobin and other venom serine proteinases. Molecular modeling shows that these sequences are located in surface loop regions, one of which is next to the catalytic site, When these sequences were replaced in TSV-PA by the equivalent batroxobin residues none generated either fibrinogen-clotting or direct fibrinogenolytic activity, Two of the replacements had little effect in general and are not critical to the specificity of TSV-PA for plasminogen. Nevertheless, the third replacement, produced by the conversion of the sequence DDE 96a-98 to NVI, significantly increased the K-m for some tripeptide chromogenic substrates and resulted in undetectable plasminogen activation, indicating the key role that the sequence plays in substrate recognition by the enzyme.

Further investigations on the relative efficiency of different shaped otter boards

Horizontal curved, vertical curved and V-shaped otter boards were studied to compare their relative efficiency under identical fishing conditions. The gear operated with V-shaped otter boards performed well followed by the gear fitted with horizontal curved boards. Vertical curved boards were found to be comparatively less efficient, but with slight modification, can be used advantageously for bottom and off bottom fishes. Fishing at various depths with horizontal curved and V-shaped otter boards at different scope ratios showed better performance of horizontal curved boards at a scope ratio of 1:6 and V-shaped otter boards at a scope ratio of 1:5.

Investigations on long distance transportation of fish. 3. Field trials with a dismantlable insulated galvanised iron container

The paper communicates the results of field trials conducted with a dismantlable insulated galvanised iron container designed and fabricated by the first two authors in their laboratory. Different varieties of fishes and different types of packing, namely, fresh iced, chilled iced and frozen were employed in the transportation experiments which were conducted from Kakinada to Howrah, Kakinada to New Delhi and Paradeep to Howrah. In all the experiments the container performed exceedingly well and has still remained in very trim condition.

Investigations on long distance transportation of fish. 4. A comparative study of the performance of expanded polystyrene slabs and multi-layer gunny (jute) fabric as insulants

A comparative study of the insulation efficiencies of expanded polystyrene slabs and multi-layer gunny fabric in long distance transportation of fresh iced fish was made. Used plywood boxes (second hand tea chests) were employed as containers and the experiments conducted between Kakinada and Calcutta. All the three insulants tried, namely, 25.4 mm thick expanded polystyrene slab, four and two layer gunny (jute) fabric, all sealed in 150 gauge polythene sheets, showed comparable insulation efficiencies, considering total bacterial counts, organoleptic qualities and TMA and TVN values of the transported fish as parameters.

Investigations on long distance transportation of fish. 5. Transportation of filleted and round seer fish (Scomberomorus sp.) from Kakinada to Calcutta by rail

Iced seer fish (Scomberomorus sp.) was transported by rail in expanded polystyrene insulated plywood boxes from Kakinada to Calcutta in round and fillet forms. While both withstood the rigors of transportation squarely, the fillets fetched only half the price of round fish in the auction conducted at the Calcutta market.

Bacteriological investigations of prawn canneries 1. Incidence of aerobic sporeformers

A detailed bacteriological survey of the prawn canneries of Cochin area was carried out to study the nature and type of micro-organisms present in the factory environs and their role in causing contamination of the canned products. About 26% of the total of 1030 strains isolated was found to be gram positive spore-formers of the Bacillus type, the cooling water being their major source. Similar types of organisms formed the major group often met with in defective canned prawn samples picked up from the factories for examination, thus establishing a correlation between bacterial characteristics and load of cooling water and can contamination.

Biochemical investigations on edible molluscs of Kerala 1. A study on the nutritional value of some bivalves

Data on the biochemical constituents and food values of five commercially important edible bivalves of Kerala, Lamellidens corrianus, Corbicuta striatella, Mytilus edulis, Vellorita cohinensis and Ostrea cucullata have been presented. Physiological significance of the variations has been discussed. Present study reveals the bivalves meat compares favourably with the common food fishes with regard to their caloric value and hence would be an excellent and economic source of nutrition for our people.

Investigations on wooden floating materials: the loss of buoyancy under continuous immersion in water and the rate of its redemption by drying

A wooden fishing float under immersion in water for long periods is liable to absorb water, the quantity of water absorbed possibly being dependent upon the physical factors like the specific gravity and the inherent property of the material, the time of soaking and the pressure acting on it. Consequently a wooden float is likely to become heavy and loss its original buoyancy. However, when the float is removed from water and dried, the lost buoyancy is regained on complete drying. The present paper is an attempt to elucidate these two important characteristics of some of the chief wooden floating materials used on the West Coast of India.