Cloning, expression, and distribution of a Ca(2+)-activated K+ channel beta-subunit from human brain.

We have cloned and expressed a Ca(2+)-activated K+ channel beta-subunit from human brain. The open reading frame encodes a 191-amino acid protein possessing significant homology to a previously described subunit cloned from bovine muscle. The gene for this subunit is located on chromosome 5 at band q34 (hslo-beta). There is no evidence for alternative RNA splicing of this gene product. hslo-beta mRNA is abundantly expressed in smooth muscle, but expression levels are low in most other tissues, including brain. Brain subregions in which beta-subunit mRNA expression is relatively high are the hippocampus and corpus callosum. The coexpression of hslo-beta mRNA together with hslo-alpha subunits in either Xenopus oocytes or stably transfected HEK 293 cells give rise to Ca(2+)-activated potassium currents with a much increased calcium and/or voltage sensitivity. These data indicate that the beta-subunit shows a tissue distribution different to that of the alpha-subunit, and in many tissues there may be no association of alpha-subunits with beta-subunits. These beta-subunits can play a functional role in the regulation of neuronal excitability by tuning the Ca2+ and/or the voltage dependence of alpha-subunits.

Distribution of endogenous type B and type D sheep retrovirus sequences in ungulates and other mammals.

The jaagsiekte sheep retrovirus (JSRV), which appears to be a type B/D retrovirus chimera, has been incriminated as the cause of ovine pulmonary carcinoma. Recent studies suggest that the sequences related to this virus are found in the genomes of normal sheep and goats. To learn whether there are breeds of sheep that lack the endogenous viral sequences and to study their distribution among other groups of mammals, we surveyed several domestic sheep and goat breeds, other ungulates, and various mammal groups for sequences related to JSRV. Probes prepared from the envelope (SU) region of JSRV and the capsid (CA) region of a Peruvian type D virus related to JSRV were used in Southern blot hybridization with genomic DNA followed by low- and high-stringency washes. Fifteen to 20 CA and SU bands were found in all members of the 13 breeds of domestic sheep and 6 breeds of goats tested. There were similar findings in 6 wild Ovis and Capra genera. Within 22 other genera of Bovidae including domestic cattle, and 7 other families of Artiodactyla including Cervidae, there were usually a few CA or SU bands at low stringency and rare bands at high stringency. Among 16 phylogenetically distant genera, there were generally fewer bands hybridizing with either probe. These results reveal wide-spread phylogenetic distribution of endogenous type B and type D retroviral sequences related to JSRV among mammals and argue for further investigation of their potential role in disease.

Intracellular polyamines mediate inward rectification of Ca(2+)-permeable alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors.

alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors that lack the glutamate receptor GluR2 subunit are Ca(2+)-permeable and exhibit inwardly rectifying current responses to kainate and AMPA. A proportion of cultured rat hippocampal neurons show similar Ca(2+)-permeable inwardly rectifying AMPA receptor currents. Inward rectification in these neurons was lost with intracellular dialysis and was not present in excised outside-out patches but was maintained in perforated-patch whole-cell recordings, suggesting that a diffusible cytoplasmic factor may be responsible for rectification. Inclusion of the naturally occurring polyamines spermine and spermidine in the recording pipette prevented loss of rectification in both whole-cell and excised-patch recordings; Mg2+ and putrescine were without effect. Inward rectification of Ca(2+)-permeable AMPA receptors may reflect voltage-dependent channel block by intracellular polyamines.

D. Certification to study French, 1796 September 5

This subseries contains one brief handwritten certification that Loammi Baldwin's son may study the French language as part of his college studies.

D. Certification to study French, 1796 September 5

This subseries contains one brief handwritten certification that Loammi Baldwin's son may study the French language as part of his college studies.

M.E. Blochii ... Systema ichthyologiae iconibus CX illustratum / post obitum auctoris opus inchoatum absoluit, correxit, interpolavit Jo. Gottlob Schneider, Saxo.

[Atlas]

Records of the Geographical Society, ca. 1796

This small blue-covered paper notebook contains four leaves with the handwritten records of the Geographical Society, an undergraduate organization at Harvard in the late 1790s. The records consist of ten handwritten "Laws of the Geographical Society" and a short list of fines dispensed on October 7th. A list of six student surnames is written on a scrap of paper and attached with pins to the notebook's inside front cover. The surnames likely correspond to six members of the Harvard Class of 1798: John Abbot (1777-1854), Isaac Adams (d. 1807), Francis Brigham (d. November 14, 1796), Humphrey Devereux (1779-1867), Joseph Emerson (1777-1833), and Artemas Sawyer (d. 1826). The notebook is undated but was presumably kept in 1795 or 1796 around the time of Brigham's death on November 14, 1796. While Brigham's surname appears in the list of fines, it is crossed out on the inside front cover.

John Winthrop's proposal respecting electrical globes and jars, ca. 1758

In this proposal, John Winthrop explains the need to replace damaged "electric globes" used in the College's collection of scientific apparatus. He states that Benjamin Franklin, at the time residing in London, was willing to seek replacement globes for the College's collection. Winthrop then proceeds to assert that the College should acquire "square bottles, of a moderate size, fitted in a wooden box, like what they call case bottles for spirits" instead of the large jars included in the scientific apparatus, because those jars cracked frequently.

Abstracts of legislation relating to Harvard College governance (manuscript notebook), ca. 1723

Octavo-sized notebook containing handwritten abstracts of Massachusetts General Court legislation between 1650 and August 24, 1723 related to Harvard governance. The volume contains informal notes with extracts and summaries of legislation that established or amended the makeup and power of the Harvard Corporation. The authors of the volume are unidentified, but the notes appear to be in two different hands. The volume was presumably created during the fellowship controversy that erupted in the early 1720s after tutors Henry Flynt, Nicholas Sever, and Thomas Robie presented a memorial to the Board of Overseers calling for the tutors' right of fellowship in the Corporation.

Poems copied by James Diman, ca. 1730-1733

Small pamphlet-sized notebook containing handwritten transcriptions of three poems copied by James Diman, likely in the early 1730s. The notebook contains "The Catholic Remedy. to ye Tune of To all you Ladies, not at land &c.," "Father Ab--y's Will. Col. Sweeper Camb: Dec. 1731," and "The Poet's Lamentation for ye Loss of his cat, w'ch he used to call his Muse" copied from the London Magazine, November 1733. The "Catholic Remedy" begins "You Peope who desire to mend / your Desperate Estate..." and includes the note, "Made upon A--D--'s goving over to take orders. The note refers to the voyage of Addington Davenport (Harvard AB 1719) to England join the priesthood of the Church of England in 1730. "Father Ab--y's Will" begins "To my dear Wife, / My joy and Life..." and was a humorous poem published in 1731 after the death of Harvard College Sweeper Matthew Abdy, and attributed to Jonathan Seccombe (Harvard AB 1728). The "Poet's Lamentation" begins "Oppress'd with Grief, in heavy strains I mourn..." and was written by Joseph Green (Harvard AB 1726) as a parody of a psalm composed by Mather Byles (Harvard AB 1725). Pages 10-12, holding part of "Father Ab--y's Will" are missing, and pages 13-15 are no longer attached to the item.

Mathematical notebook attributed to Thaddeus Mason Harris, ca. 1787

Hardcover notebook containing handwritten transcriptions of rules, cases, and examples from 18th century mathematical texts. The author and purpose of the volume is unclear, though it has been connected with Thaddeus Mason Harris (Harvard AB 1787). Most of the entries include questions and related answers, suggesting the notebook was used as a manuscript textbook and workbook. The extracts appear to be copied from John Dean's " Practical arithmetic" (published in 1756 and 1761), Daniel Fenning's "The young algebraist's companion" (published in multiple editions beginning in 1750), and Martin Clare's "Youth's introduction to trade and business" (extracts first included in 1748 edition).

Thaddeus Mason Harris' working documents regarding subject-based library catalog, ca. 1790

Thaddeus Mason Harris, who served as interim librarian of the Harvard College Library in 1787 and as its librarian from 1791 through 1793, is believed to have created these notes while helping compile the library's first printed subject-based catalog. The catalog, Catalogus Bibliothecae Harvardianae Cantabrigiae Nov-Anglorum, was published in 1790 and represented a significant change in approach to the cataloging of the library's collections, which had formerly been cataloged alphabetically. These documents, many of them on small scraps of paper, contain the titles and bibliographic information of books on a range of topics, from "Anatomici" to "Rhetorica."

The invention of letters annotated by Charles Pinckney Sumner, ca. 1795-1796

Interleaved second-edition copy of Robert Treat Paine's poem "The Invention of Letters" with handwritten excerpts of 18th century poetry copied by Charles Pinckney Sumner. The excerpts appear to be verses alluded to, or emulated, by Paine in the poem. For example, Paine's verse includes "Beneath the shade, which Freedom's oak displays" and Sumner on the opposite page quoted Alexander Pope's poetry, "Beneath the shade a spreading beech displays." The excerpts include poetry by Alexander Pope, James Thompson, Robert Dodsley, William Falconer, William Hayley, Samuel Rogers, Richard Brinsley Sheridan, Thomas Gray, and John Denham.

Rabbinical manuscripts of Judah Monis, ca. 1700s

This leather-bound volume contains ten handwritten Hebrew texts presumably compiled by Judah Monis in the early 18th century. The pieces range from three to 150 pages on different sized leaves and appear to be in multiple hands. The last page of the volume has the struck-through inscription, "Judah Monis' Book" and accompanies a 44-page text. The texts are unattributed and undated, but have been identified as transcriptions of cabalistic writings and include a short biography of Isaac Luria (1533-1572) and extracts from the work of Luria, Hayyim ben Joseph Vital, Jacob ben Hayyim Zemah, Abraham ben Isaac of Granada, and Naphtali Bachrach. The transcriptions appear to be unattributed and undated.