Human semaphorins A(V) and IV reside in the 3p21.3 small cell lung cancer deletion region and demonstrate distinct expression patterns.

Semaphorins and collapsins make up a family of conserved genes that encode nerve growth cone guidance signals. We have identified two additional members of the human semaphorin family [human semaphorin A(V) and human semaphorin IV] in chromosome region 3p21.3, where several small cell lung cancer (SCLC) cell lines exhibit homozygous deletions indicative of a tumor suppressor gene. Human semaphorin A(V) has 86% amino acid homology with murine semaphorin A, whereas semaphorin IV is most closely related to murine semaphorin E, with 50% homology. These semaphorin genes are approximately 70 kb apart flanking two GTP-binding protein genes, GNAI-2 and GNAT-1. In contrast, other human semaphorin gene sequences (human semaphorin III and homologues of murine semaphorins B and C) are not located on chromosome 3. Human semaphorin A(V) is translated in vitro into a 90-kDa protein, which accumulates at the endoplasmic reticulum. The human semaphorin A(V) (3.4-kb mRNA) and IV (3.9- and 2.9-kb mRNAs) genes are expressed abundantly but differentially in a variety of human neural and nonneural tissues. Human semaphorin A(V) was expressed in only 1 out of 23 SCLCs and 7 out of 16 non-SCLCs, whereas semaphorin IV was expressed in 19 out of 23 SCLCs and 13 out of 16 non-SCLCs. Mutational analysis in semaphorin A(V) revealed mutations (germ line in one case) in 3 of 40 lung cancers. Our data suggest the need to determine the function of human semaphorins A(V) and IV in nonneural tissues and their role in the pathogenesis of lung cancer.

Electrochemical reactions of catechol, methylcatechol and dopamine at tetrahedral amorphous carbon (ta-C) thin film electrodes

The electrochemical reactions of dopamine, catechol and methylcatechol were investigated at tetrahedral amorphous carbon (ta-C) thin film electrodes. In order to better understand the reaction mechanisms of these molecules, cyclic voltammetry with varying scan rates was carried out at different pH values in H2SO4 and PBS solutions. The results were compared to the same redox reactions taking place at glassy carbon (GC) electrodes. All three catechols exhibited quasi-reversible behavior with sluggish electron transfer kinetics at the ta-C electrode. At neutral and alkaline pH, rapid coupled homogeneous reactions followed the oxidation of the catechols to the corresponding o-quinones and led to significant deterioration of the electrode response. At acidic pH, the extent of deterioration was considerably lower. All the redox reactions showed significantly faster electron transfer kinetics at the GC electrode and it was less susceptible toward surface passivation. An EC mechanism was observed for the oxidation of dopamine at both ta-C and GC electrodes and the formation of polydopamine was suspected to cause the passivation of the electrodes.

Mean arterial pressure and vasopressor load after out-of-hospital cardiac arrest: Associations with one-year neurologic outcome

THE AIM OF THE STUDY There are limited data on blood pressure targets and vasopressor use following cardiac arrest. We hypothesized that hypotension and high vasopressor load are associated with poor neurological outcome following out-of-hospital cardiac arrest (OHCA). METHODS We included 412 patients with OHCA included in FINNRESUSCI study conducted between 2010 and 2011. Hemodynamic data and vasopressor doses were collected electronically in one, two or five minute intervals. We evaluated thresholds for time-weighted (TW) mean arterial pressure (MAP) and outcome by receiver operating characteristic (ROC) curve analysis, and used multivariable analysis adjusting for co-morbidities, factors at resuscitation, an illness severity score, TW MAP and total vasopressor load (VL) to test associations with one-year neurologic outcome, dichotomized into either good (1-2) or poor (3-5) according to the cerebral performance category scale. RESULTS Of 412 patients, 169 patients had good and 243 patients had poor one-year outcomes. The lowest MAP during the first six hours was 58 (inter-quartile range [IQR] 56-61) mmHg in those with a poor outcome and 61 (59-63) mmHg in those with a good outcome (p<0.01), and lowest MAP was independently associated with poor outcome (OR 1.02 per mmHg, 95% CI 1.00-1.04, p=0.03). During the first 48h the median (IQR) of the TW mean MAP was 80 (78-82) mmHg in patients with poor, and 82 (81-83) mmHg in those with good outcomes (p=0.03) but in multivariable analysis TWA MAP was not associated with outcome. Vasopressor load did not predict one-year neurologic outcome. CONCLUSIONS Hypotension occurring during the first six hours after cardiac arrest is an independent predictor of poor one-year neurologic outcome. High vasopressor load was not associated with poor outcome and further randomized trials are needed to define optimal MAP targets in OHCA patients.

1966 UM football players

back row L-R: Michael Bass, Rick Volk, Frank Nunley, Dennis Morgan, Rick Sygar, John Rowser. front row L-R: terry Salmi, Kenneth Wright, David Porter, Richard Williamson, Roger Rosema.

400 outstanding women of the world and costumology of their time /

Mode of access: Internet.

Im Vaterhause; sozialer Roman.

Mode of access: Internet.

Varia sacra.

fasc.I., I.Annonymi chiliastae in Matthaeum XXIV fragmenta. Accedunt tractus De tribus mensuris et De Petro apostolo.-II. Piccoli supplementi agliscritti dei dottori Cappadoci e di S. Cirillo Alessandrino.-III. I fragmenti inediti dell'antica versione latina del commentario di Teodoro Mopsuesteno sui Salmi.

I Libri poetici della Bibbia : tradotti dall'ebraico originale ed adattati al gusto della poesia italiana ... /

Mode of access: Internet.

I Libri poetici della Bibbia : tradotti dall'ebraico originale ed adattati al gusto della poesia italiana colle note ed osservazioni critiche, politiche e morali e colle dissertazioni ... /

Mode of access: Internet.

Gotthold Ephraim Lessings sämtliche schriften.

Volumes 12-21 have imprint: Leipzig, G. J. Göschen; v. 22: Berlin und Leipzig, G. J. Göschen; v. 23: Berlin und Leipzig, W. de Gruyter & co.

Familien-bibliothek der deutschen classiker ...

No vol. numbered 7; two volumes numbered 2.

Lessing's werke ...

Theil 7 edited by Georg Zimmermann; th. 8, by Robert Pilger; th. 9-10, 12, 19-20, by Carl Chritian Redlich; th. 11, abth, 2, by Robert Boxberger; th. 13, abth, 1, by Emil Grosse; th. 13, abth, 2, by Alfred Schöne; th. 14-18, by Christian Gross, cf. Goedeke, Grundriss zur geschichte der deutschen dichtung, 3. aufl., vol. iv, p. 345.

G. E. Lessing als reformator der deutschen literatur, bargestellt /

V. 1. Lessing reformatorische bedeutung. Minna von Barnhelm. Faust. Emilia Galotti. 2. aufl.--v. 2. Nathan der weise. Der idee und die charaktere der dichtung. 5. aufl.

Tsinat Daṿid : maʾamar ḥoḳer zeman huledet sefer Tehilim... : ṿe-nosaf elaṿ Tiferet shire ḳodesh /

Added t.p. has title also in German.