Inhibition of AP-1 binding and transcription by gold and selenium involving conserved cysteine residues in Jun and Fos.

Gold(I) salts and selenite, which have diverse therapeutic and biological effects, are noted for their reactivity with thiols. Since the binding of Jun-Jun and Jun-Fos dimers to the AP-1 DNA binding site is regulated in vitro by a redox process involving conserved cysteine residues, we hypothesized that some of the biological actions of gold and selenium are mediated via these residues. In electrophoretic mobility-shift analyses, AP-1 DNA binding was inhibited by gold(I) thiolates and selenite, with 50% inhibition occurring at approximately 5 microM and 1 microM, respectively. Thiomalic acid had no effect in the absence of gold(I), and other metal ions inhibited at higher concentrations, in a rank order correlating with their thiol binding affinities. Cysteine-to-serine mutants demonstrated that these effects of gold(I) and selenite require Cys272 and Cys154 in the DNA-binding domains of Jun and Fos, respectively. Gold(I) thiolates and selenite did not inhibit nonspecific protein binding to the AP-1 site and were at least an order of magnitude less potent as inhibitors of sequence-specific binding to the AP-2, TFIID, or NF1 sites compared with the AP-1 site. In addition, 10 microM gold(I) or 10 microM selenite inhibited expression of an AP-1-dependent reporter gene, but not an AP-2-dependent reporter gene. These data suggest a mechanism regulating transcription factor activity by inorganic ions which may contribute to the known antiarthritic action of gold and cancer chemoprevention by selenium.

The genera of fishes ... : A contribution to the stability of scientific nomenclature / By David Starr Jordan.

pt. 1-2

The Year-book of agriculture, or, The annual of agricultural progress and discovery / [edited] by David A. Wells.

1855-1856

The genera of fishes ... : A contribution to the stability of scientific nomenclature / By David Starr Jordan.

pt. 3-4

Trachelyichthys exilis, a new species of catfish (Pisces, Auchenipteridae) from Peru / David W. Greenfield and Garrett S. Glodek.

v.72:no.3(1977)

New agnathous fishes from the Pennsylvanian of Illinois / David Bardack -- and Eugene S. Richardson, Jr. --

v.33:no.26(1977)

Morphology and relationships of saurocephalid fishes / [by] David Bardack -- and Gloria Sprinkle --.

v.16:no.12(1969)

Paracanthopterygian and acanthopterygian fishes from the Upper Cretaceous of Kansas / David Bardack --.

v.33:no.20(1976)

Ptycholepis marshi Newberry : a chondrostean fish from the Newark group of Eastern North America / Bobb Schaeffer --, David H. Dunkle --, Nicholas G. McDonald --.

v.33:no.12(1975)

The non-therapsid reptiles of the Lufeng Basin, Yunnan, China / David Jay Simmons -- [Edited by Edward G. Nash]

v.15:no.1(1965)

Book of monsters, by David and Marian Fairchild. Portraits and biographies of a few of the inhabitiants of woodland and meadow.

1914

Letter from David Phips to Colonel Jonathan Snelling regarding escort of Governor Hutchinson to Harvard Commencement, 1773 July 12

David Phips wrote this letter to Colonel Jonathan Snelling from Cambridge on July 12, 1773, to inform him that Massachusetts Governor Thomas Hutchinson had requested the accompaniment of guards during his travels from Milton to Cambridge on July 21, 1773, to attend the Harvard College Commencement exercises. In the letter, Phips informs Snelling that he has issued warrants to the guards, instructing them to congregate at the Sign of the Grey Hound in Roxbury, Massachusetts at eight o'clock on the morning of the 21st. He explains that twelve other men will march, under the command of Sub-Brigadier Sumner, to the Governor's home in Milton to escort him to Roxbury, where the larger party will assemble. These heightened security measures were certainly prompted by political unrest, although this is not stated explicitly in the letter. Phips concludes by saying: "I shall order a dinner for us at Bradish's, where I hope to have the pleasure to dine with you."

Journal of Charles Coffin and David Gurney, 1781-1785

This diary appears to have been kept by two different students, both members of the Harvard College class of 1785. The first two pages contain entries made by a student named David, believed to be David Gurney because the entries relate to the freshman curriculum and Gurney was the only student named David who was a freshman in 1781. Gurney originally titled the volume "A Journal or Diary of my concerns in College of important matters." He made entries from August 28 through October 21, 1781, recording his lessons on Virgil, Tully, Homer, the Greek Testament, Hebrew grammar, English author John Ash's "Grammar," and a text called "The Art of Speaking." At the top of one of the pages recounting these studies, Gurney wrote in large, bold letters: "About how I misspent my precious time." Charles Coffin's entries begin on October 25, 1781 and fill the bulk of the journal. Coffin kept this diary while a student at Harvard College from 1781 to 1785. Although most of Coffin's entries are written in Latin, an account of his July 1781 examination for admission to the College is in English.