Comparison of the depot effect and immunogenicity of liposomes based on dimethyldioctadecylammonium (DDA), 3β-[N-(N',N'-Dimethylaminoethane)carbomyl] cholesterol (DC-Chol), and 1,2-Dioleoyl-3-trimethylammonium propane (DOTAP):prolonged liposome retention mediates stronger Th1 responses

The immunostimulatory capacities of cationic liposomes are well-documented and are attributed both to inherent immunogenicity of the cationic lipid and more physical capacities such as the formation of antigen depots and antigen delivery. Very few studies have however been conducted comparing the immunostimulatory capacities of different cationic lipids. In the present study we therefore chose to investigate three of the most well-known cationic liposome-forming lipids as potential adjuvants for protein subunit vaccines. The ability of 3ß-[N-(N',N'-dimethylaminoethane)carbomyl] cholesterol (DC-Chol), 1,2-dioleoyl-3-trimethylammonium propane (DOTAP), and dimethyldioctadecylammonium (DDA) liposomes incorporating immunomodulating trehalose dibehenate (TDB) to form an antigen depot at the site of injection (SOI) and to induce immunological recall responses against coadministered tuberculosis vaccine antigen Ag85B-ESAT-6 are reported. Furthermore, physical characterization of the liposomes is presented. Our results suggest that liposome composition plays an important role in vaccine retention at the SOI and the ability to enable the immune system to induce a vaccine specific recall response. While all three cationic liposomes facilitated increased antigen presentation by antigen presenting cells, the monocyte infiltration to the SOI and the production of IFN-? upon antigen recall was markedly higher for DDA and DC-Chol based liposomes which exhibited a longer retention profile at the SOI. A long-term retention and slow release of liposome and vaccine antigen from the injection site hence appears to favor a stronger Th1 immune response.

Semantic search meets the Web

While semantic search technologies have been proven to work well in specific domains, they still have to confront two main challenges to scale up to the Web in its entirety. In this work we address this issue with a novel semantic search system that a) provides the user with the capability to query Semantic Web information using natural language, by means of an ontology-based Question Answering (QA) system [14] and b) complements the specific answers retrieved during the QA process with a ranked list of documents from the Web [3]. Our results show that ontology-based semantic search capabilities can be used to complement and enhance keyword search technologies.

Foreword to the special issue on European conference on optical communications (ECOC 2014)

The articles in this special section were presented at the European Conference on Optical Communications (ECOC) that was held on on 21???25 September 2014 in Cannes, France

Conference on Geometry and Mathematical Physics, Bulgaria, Zlatograd, 28.08-01.09.2005

List of Participants

A Conference on The 200th Anniversary of the Birth of Gertrud is Gómez de Avellaneda: A Celebration of Nineteenth-Century Cuban Literature

This flyer promotes the event "A Conference on The 200th Anniversary of the Birth of Gertrud is Gómez de Avellaneda: A Celebration of Nineteenth-Century Cuban Literature" cosponsored by the Cuban Research Institute and the Department of Modern Languages at Florida International University.

Ninth Conference on Cuban and Cuban-American Studies "Dispersed Peoples: The Cuban and Other Diasporas"

This flyer promotes the Ninth Conference on Cuban and Cuban-American Studies "Dispersed Peoples: The Cuban and Other Diasporas" , cosponsored by FlU's Cuban Research Institute, FlU African and African Diaspora Studies Program, Center for the Humanities in an Urban Environment, Exile Studies Program, and WPBT2. This event was held May 23, 2013 - May 25, 2013.