Identification and relative quantification of tyrosine nitration in a model peptide using two-dimensional infrared spectroscopy

Nitration of tyrosine in proteins and peptides is a post-translational modification that occurs under conditions of oxidative stress. It is implicated in a variety of medical conditions, including neurodegenerative and cardiovascular diseases. However, monitoring tyrosine nitration and understanding its role in modifying biological function remains a major challenge. In this work, we investigate the use of electron-vibration-vibration (EVV) two-dimensional infrared (2DIR) spectroscopy for the study of tyrosine nitration in model peptides. We demonstrate the ability of EVV 2DIR spectroscopy to differentiate between the neutral and deprotonated states of 3-nitrotyrosine, and we characterize their spectral signatures using information obtained from quantum chemistry calculations and simulated EVV 2DIR spectra. To test the sensitivity of the technique, we use mixed-peptide samples containing various levels of tyrosine nitration, and we use mass spectrometry to independently verify the level of nitration. We conclude that EVV 2DIR spectroscopy is able to provide detailed spectroscopic information on peptide side-chain modifications and to detect nitration levels down to 1%. We further propose that lower nitration levels could be detected by introducing a resonant Raman probe step to increase the detection sensitivity of EVV 2DIR spectroscopy. (Graph Presented).

Optical immersion of mid-infrared LEDs and photodiodes for gas-sensing applications

The high gains in performance predicted for optical immersion are difficult to achieve in practice due to total internal reflection at the lens/detector interface. By reducing the air gap at this interface optical tunneling becomes possible and the predicted gains can be realized in practical devices. Using this technique we have demonstrated large performance gains by optically immersing mid-infrared heterostructure InA1Sb LEDs and photodiodes using hypershperical germanium lenses. The development of an effective method of optical immersion that gives excellent optical coupling has produced a photodiode with a peak room temperature detectivity (D*) of 5.3 x 109 cmHz½W-1 at λpeak=5.4μm and a 40° field of view. A hyperspherically immersed LED showed a f-fold improvement in the external efficiency, and a 3-fold improvement in the directionality compared with a conventional planar LED for f/2 optical systems. The incorporation of these uncooled devices in a White cell produced a NO2 gas sensing system with 2 part-per-million sensitivity, with an LED drive current of <5mA. These results represent a significant advance in the use of solid state devices for portable gas sensing systems.

Distributed Visualization Systems in Remote Sensing Data Processing Grid

Implementation of GEOSS/GMES initiative requires creation and integration of service providers, most of which provide geospatial data output from Grid system to interactive user. In this paper approaches of DOS- centers (service providers) integration used in Ukrainian segment of GEOSS/GMES will be considered and template solutions for geospatial data visualization subsystems will be suggested. Developed patterns are implemented in DOS center of Space Research Institute of National Academy of Science of Ukraine and National Space Agency of Ukraine (NASU-NSAU).

Chalcogenide suspended-core fibers for supercontinuum generation in the mid-infrared

Chalcogenide suspended core fibers are a valuable solution to obtain supercontinuum generation of light in the mid-infrared, thanks to glass high transparency, high index contrast, small core diameter and widely-tunable dispersion. In this work the dispersion and nonlinear properties of several chalcogenide suspended core mi-crostructured fibers are numerically evaluated, and the effects of all the structural parameters are investigated. Optimization of the design is carried out to provide a fiber suitable for wide-band supercontinuum generation in the mid-infrared.

Review and Outlooks of the Means for Visualization of Syntax Semantics and Source Code. Procedural and Object Oriented Paradigm – Differences

In the article, we have reviewed the means for visualization of syntax, semantics and source code for programming languages which support procedural and/or object-oriented paradigm. It is examined how the structure of the source code of the structural and object-oriented programming styles has influenced different approaches for their teaching. We maintain a thesis valid for the object-oriented programming paradigm, which claims that the activities for design and programming of classes are done by the same specialist, and the training of this specialist should include design as well as programming skills and knowledge for modeling of abstract data structures. We put the question how a high level of abstraction in the object-oriented paradigm should be presented in simple model in the design stage, so the complexity in the programming stage stay low and be easily learnable. We give answer to this question, by building models using the UML notation, as we take a concrete example from the teaching practice including programming techniques for inheritance and polymorphism.

Mid-infrared passively switched pulsed dual wavelength Ho3+ -doped fluoride fiber laser at 3 μm and 2 μm

Cascade transitions of rare earth ions involved in infrared host fiber provide the potential to generate dual or multiple wavelength lasing at mid-infrared region. In addition, the fast development of saturable absorber (SA) towards the long wavelengths motivates the realization of passively switched mid-infrared pulsed lasers. In this work, by combing the above two techniques, a new phenomenon of passively Q-switched ~3 μm and gain-switched ~2 μm pulses in a shared cavity was demonstrated with a Ho3+-doped fluoride fiber and a specifically designed semiconductor saturable absorber (SESAM) as the SA. The repetition rate of ~2 μm pulses can be tuned between half and same as that of ~3 μm pulses by changing the pump power. The proposed method here will add new capabilities and more flexibility for generating mid-infrared multiple wavelength pulses simultaneously that has important potential applications for laser surgery, material processing, laser radar, and free-space communications, and other areas.

Interactive 3D Visualization of Bézier Curves using Java Open Graphics Library (JOGL)

We present a new program tool for interactive 3D visualization of some fundamental algorithms for representation and manipulation of Bézier curves. The program tool has an option for demonstration of one of their most important applications - in graphic design for creating letters by means of cubic Bézier curves. We use Java applet and JOGL as our main visualization techniques. This choice ensures the platform independency of the created applet and contributes to the realistic 3D visualization. The applet provides basic knowledge on the Bézier curves and is appropriate for illustrative and educational purposes. Experimental results are included.

Visualization of Problems’ Solution

Джурджица Такачи - В доклада се разглеждат дидактически подходи за решаване на задачи, упражнения и доказване на теореми с използване на динамичен софтуер, по-специално – с вече широко разпространената система GeoGebra. Въз основа на концепция-та на Пойа се анализира използването на GeoGebra като когнитивно средство за решаване на задачи и за обсъждане на техни възможни обобщения.

3D Reconstruction and Digital Visualization of the South of the Royal Palace in Great Preslav

The report presents the film 10th century. The South of the Royal Palace in Great Preslav. It consists of two parts – 10th century. The Royal Palace in Great Prelsav. The Square with the Pinnacle and The Ruler’s Lodgings. 3D and virtual reconstructions of an architectural ensemble – part of the Preslav Royal Court unearthed during archaeological researches are used in the film. 3D documentaries have already gained popularity around the world and are well received by both scholars and the public at large. One of the distinguished tourist destinations in Bulgaria is Great Preslav – capital of the mediaeval Bulgarian state and a significant cultural center of the European Southeast in 9th–10th centuries, too. The first part of the film is created with the financial support of America for Bulgaria Foundation and the second – with the funding of Bulgarian National Science Fund at the Ministry of Education, Youth and Science. A team of almost 20 members worked on the film, including computer specialists, professional actors, and translators in the four main European languages – English, German, French and Russian, Trima Sound Recording Studio. In the first part of the 3D film are shown a segment of the Royal Palace, the square with the water pinnacle and the adjacent buildings – an important structural element of the town-planning of the Preslav Court center in the 10th century. In the second part the accent is the southern part of the Royal Palace in Great Preslav, where the personal residence of the Preslav ruler’s dynasty is situated. The work on the virtual reconstruction was done by Virtual Archaeology club at the Mathematical School, Shumen. Due to the efforts of its members it is now clear how the square in front of the southern gate looked like.

Interactive Visualization of the Building of University of Economics – Varna via 3D Modeling

The object of this paper is presenting the University of Economics – Varna, using a 3D model with 3Ds MAX. Created in 1920, May 14, University of Economics - Varna is a cultural institution with a place and style of its own. With the emergence of the three-dimensional modeling we entered a new stage of the evolution of computer graphics. The main target is to preserve the historical vision, to demonstrate forward-thinking and using of future-oriented approaches.

Demo: Shield UI JavaScript Chart - A Flexible HTML5 Data Visualization Component

Shield UI’s advanced framework for creating rich charts and graphs is the first of a line of data visualization components, giving web developers the power for embedding rich graphics in their web projects with minimum effort. Built with HTML, CSS3 and packaged as a jQuery plugin, the library has full support for legacy and modern desktop web browsers, as well as the latest mobile devices.

Mathematical competence for information visualization by correspondence analysis methods

Report published in the Proceedings of the National Conference on "Education and Research in the Information Society", Plovdiv, May, 2016

Fibre grating filters for suppression of near infrared OH emission lines

Here we present the design and fabrication of multi-notch optical fibre Bragg gratings for suppressing OH emission lines in the near infrared spectra of the night sky for astrophysical applications. We demonstrate a novel approach of fabricating 2, 3 and 5-notch filters using the phase mask technology, which show a good match with the model.

Highly nonlinear chalcogenide suspended-core fibers for applications in the mid-infrared

Due to their unique dispersion and nonlinear properties, chalcogenide suspended-core fibers, characterized by a few micrometer-sized core suspended between large air-holes by few small glaß struts, are excellent candidates for mid-infrared applications. In the present study the influence of the main croß-section characteristics of the chalcogenide suspended-core fibers on the dispersion curve and on the position of the zero-dispersion wavelength has been thoroughly analyzed with a full-vector modal solver based on the finite element. In particular, the design of suspended-core fibers made of both As2S3 and As2Se3 has been optimized to obtain dispersion properties suitable for the supercontinuum generation in the mid-infrared.