Phosphoproteome analysis of mouse liver using immobilized metal affinity purification and linear ion trap mass spectrometry

Since protein phosphorylation is a dominant mechanism of information transfer in cells, there is a great need for methods capable of accurately elucidating sites of phosphorylation. In recent years mass spectrometry has become an increasingly viable alternative to more traditional methods of phosphorylation analysis. The present study used immobilized metal affinity chromatography (IMAC coupled with a linear ion trap mass spectrometer to analyze phosphorylated proteins in mouse liver. A total of 26 peptide sequences defining 26 sites of phosphorylation were determined. Although this number of identified phosphoproteins is not large, the approach is still of interest because a series of conservative criteria were adopted in data analysis. We note that, although the binding of non-phosphorylated peptides to the IMAC column was apparent, the improvements in high-speed scanning and quality of MS/MS spectra provided by the linear ion trap contributed to the phosphoprotein identification. Further analysis demonstrated that MS/MS/MS analysis was necessary to exclude the false-positive matches resulting from the MS/MS experiments, especially for multiphosphorylated peptides. The use of the linear ion trap considerably enabled exploitation of nanoflow-HPLC/MS/MS, and in addition MS/MS/MS has great potential in phosphoproteome research of relatively complex samples. Copyright (C) 2004 John Wiley Sons, Ltd.

Capillary array electrophoresis with confocal fluorescence rotary scanner

A capillary array electrophoresis system with rotary corifocal fluorescence scanner was reported. High speed direct current rotary motor combined with a rotary encoder and the reflection mirror has been designed to direct exactly the excitation laser beam. to the array of capillaries, which are symmetrically distributed around the motor. The rotary encoder is introduced to accurately orient the position of each capillary and its output signal triggers the data acquiring system to record. the fluorescence signal corresponding to each capillary. Separations of several amino acids are demonstrated by eight-channel capillary array electrophoresis built by ourselves.

一种高速安全反向代理服务器的设计与实现

安全反向代理服务器架设在真实网页服务器与用户浏览器之间靠近真实服务器的一侧,通过传输层安全协议保障用户与真实服务器之间的通信.作为基于传输层安全协议的虚拟专用网服务器的一个最重要的组成部分,安全反向代理有着极大的商业价值和技术含量.在FreeBSD-6.3的基础上设计并实现了一个与传统TCP/IP并行的TCP/IP跳转表.基于TCP/IP跳转表设计并实现了一种多加速卡调度算法.设计并实现了直接从加速卡队列获取数据包的代理转发协议栈.设计并实现了基于后台真实服务器反馈的负载均衡算法.测试表明,由这些算法和协议栈组成的高速安全反向代理服务器新进连接数达到了国内领先水平.

实行三个基本转变促进黄土丘陵区农业和经济持续发展

依据黄土丘陵区几十年治理开发的正反两方面经验 ,认为该地区要真正抓住西部大开发大好机遇 ,迅速改变落后面貌 ,实现农业和经济的快速及可持续发展 ,必须在指导思想和农业生产上实现三个根本转变。即 :变以粮为主的广种薄收为高效的农林果牧综合发展 ;变自给自足的小农经济为以商品生产为主的集约经营 ;变生态经济相悖论为生态经济统一观

Wear mechanism map of uncoated HSS tools during drilling die-cast magnesium alloy

A wear mechanism map of uncoated high-speed steel (HSS) tools was constructed under the conditions of dry-drilling die-cast magnesium alloys. Three wear mechanisms appear in the map based on the microanalysis of drilled HSS tools by SEM, including adhesive wear, abrasive wear and diffusion wear. In the map, there exists a minor wear region which is called "safety zone". This wear mechanism map will be a good reference for choosing suitable drilling parameters when drilling die-cast magnesium alloys.

Characterization of the microstructure of biaxially oriented polypropylene using preparative temperature-rising elution fractionation

Two commercial biaxially oriented polypropylene (BOPP) resins, resin A and resin B, having different processing properties, were fractionated by preparative temperature-rising elution fractionation (TREF). The TREF fractions were further characterized by gel permeation chromatography (GPC), gel permeation chromatography coupled with light scattering (GPC-LS), wide-angle X-ray diffraction (WAXD), and differential scanning calorimetry (DSC). GPC-LS did not find visible long-chain branching in either resin A or B. The results from TREF and DSC indicate that the fractional melting parameter f(T) may be used to predict the profile of the TREF cumulative weight distribution curve. GPC results show that the molecular weights of the fractions tend to increase with elution temperature. WAXD and DSC data show that the crystallinity of fractions does not increase monotonically with increase of elution temperature. There appears to be a maximum in the plot of crystallinity versus elution temperature. The high-speed BOPP resin A has a lower isotacticity but a homogeneous isotacticity distribution and a higher molecular weight but a broader molecular weight distribution than resin B.

S／T曲线速度规划在定点DSP上的实现

本文将S／T曲线速度规划的思想引入全数字伺服驱动系统中,通过提高速度的平滑性,特别是高速启、制动状态,来提高伺服系统的整体控制性能。基于定点数字信号处理器DSP芯片对提出的算法进行了实现。由于定点运算的限制,算法在实现中需要进行特殊的处理,本文对此进行了研究,并提出了一种余码补偿方案。实验研究表明,使用本文提出的方法可以提高系统运行的平稳性和控制的精确度。

面向实时控制任务的高速数据采集存储方法

提出一种基于CAN总线,面向实时控制任务的高速数据采集存储方法.该方法将数据实时采集、系统实时控制、数据存储与显示分配在相互独立的处理单元中实现,处理单元之间通过独立的CAN网络进行通信.该数据采集方法解决了数据实时采集与系统实时控制之间的矛盾.利用队列技术,该方法解决了数据存储与数据实时显示之间的矛盾.该数据采集存储方法已成功应用到某大型仿真系统中.

基于非线性修正的多线阵像机的空间定位

介绍了一种基于多线阵像机构成的视觉空间定位系统.该系统利用线阵像机的快速性与高分辨率的特点,采用了非平行空间投影面相交定位的基本原理,利用几何投影关系定位求解的方法,实现了多线阵像机视觉系统的空间定位.并提出了多线阵像机的神经网络非线性修正方法,使修正后的PSD能在较宽的位置范围内输出高线性度的信号.实验结果表明,基于非线性修正的多线阵像机位姿测量系统简化了立体视觉空间定位计算的复杂性,在定位精度、定位范围和采样速度上均达到了良好效果.

X射线在介质中的剂量分布计算

研究了放射治疗中X射线在介质中的输运过程,编程实现了基于蒙特卡罗方法的剂量计算．并在便于图形处理的软件Matlab中对光子输运结果进行了可视化处理．对X射线在均匀介质和菲均匀介质中的蒙特卡罗模拟结果与实测结果、其他蒙特卡罗软件模拟结果进行了比较,结果符合较好．实验结果表明该方法既可以获得很快的仿真速度,又能得到精确直观的剂量计算结果,为提高放射治疗水平具有重要的指导意义和应用价值．

一种基于神经网络的视觉定位系统

本文提出了一种基于人工神经网络的多线阵相机系统标定与 3D定位方法 ,并应用于基于多线阵相机构成的视觉空间定位系统 .该视觉定位系统利用了线阵相机的快速性与高分辨率的特点 ,非平行空间投影面相交定位的基本原理 ,实现了这种结构下快速、高精度空间定位 .实验表明 ,人工神经网络的定位方法简化了多线阵视觉定位系统标定与定位计算的复杂性 ,在定位精度上达到了良好效果 .为机器人位置反馈控制提供了有效的技术途径

高速重载工业机器人臂机构研究

研究出一种新的机器人臂机构———“并联放大”复合臂机构 ,介绍了其机构原理、结构设计及测试实验。实验表明 :载荷 2 5kg时 ,臂机构末端运动速度可达到 10m/s ,该臂机构具有高速运动及抗冲击振动的能力。

一种水面救助机器人

本文介绍一种能在恶劣海况下 ,向遇险船舶送递导引缆绳和向落水人员快速送递救生圈的水面救助机器人 .该机器人具有抗涌浪能力强、体积小、重量轻、速度快以及机动灵活的特点 ,还可广泛应用于其他领域

关于参数规划在自治智能移动式机器人中实际应用的探讨

机器人行走空间的复杂性决定了导航监控系统不仅为机器人提供行走路线,还要依据预先得到的先验数据与知识告诉机器人不同行走段的障碍分布、环境边界走向及约束程度,按照载体的几何形状,运动特性选择合适的速度、加速度,保证在安全的前提下最快完成行走.本文讨论了提取影响机器人当前行走的障碍、环境边界算法和不同行走速度段的划分与关键点抽取方法,讨论了各种速度段的分离、合并、高速行走段的插补和过短行走段的平滑滤除等等.