Proximodistal identity during vertebrate limb regeneration is regulated by Meis homeodomain proteins

The mechanisms by which cells obtain instructions to precisely re-create the missing parts of an organ remain an unresolved question in regenerative biology. Urodele limb regeneration is a powerful model in which to study these mechanisms. Following limb amputation, blastema cells interpret the proximal-most positional identity in the stump to reproduce missing parts faithfully. Classical experiments showed the ability of retinoic acid (RA) to proximalize blastema positional values. Meis homeobox genes are involved in RA-dependent specification of proximal cell identity during limb development. To understand the molecular basis for specifying proximal positional identities during regeneration, we isolated the axolotl Meis homeobox family. Axolotl Meis genes are RA-regulated during both regeneration and embryonic limb development. During limb regeneration, Meis overexpression relocates distal blastema cells to more proximal locations, whereas Meis knockdown inhibits RA proximalization of limb blastemas. Meis genes are thus crucial targets of RA proximalizing activity on blastema cells.

A synthetic biology-based device prevents liver injury in mice

BACKGROUND & AIMS The liver performs a panoply of complex activities coordinating metabolic, immunologic and detoxification processes. Despite the liver's robustness and unique self-regeneration capacity, viral infection, autoimmune disorders, fatty liver disease, alcohol abuse and drug-induced hepatotoxicity contribute to the increasing prevalence of liver failure. Liver injuries impair the clearance of bile acids from the hepatic portal vein which leads to their spill over into the peripheral circulation where they activate the G-protein-coupled bile acid receptor TGR5 to initiate a variety of hepatoprotective processes. METHODS By functionally linking activation of ectopically expressed TGR5 to an artificial promoter controlling transcription of the hepatocyte growth factor (HGF), we created a closed-loop synthetic signalling network that coordinated liver injury-associated serum bile acid levels to expression of HGF in a self-sufficient, reversible and dose-dependent manner. RESULTS After implantation of genetically engineered human cells inside auto-vascularizing, immunoprotective and clinically validated alginate-poly-(L-lysine)-alginate beads into mice, the liver-protection device detected pathologic serum bile acid levels and produced therapeutic HGF levels that protected the animals from acute drug-induced liver failure. CONCLUSIONS Genetically engineered cells containing theranostic gene circuits that dynamically interface with host metabolism may provide novel opportunities for preventive, acute and chronic healthcare. LAY SUMMARY Liver diseases leading to organ failure may go unnoticed as they do not trigger any symptoms or significant discomfort. We have designed a synthetic gene circuit that senses excessive bile acid levels associated with liver injuries and automatically produces a therapeutic protein in response. When integrated into mammalian cells and implanted into mice, the circuit detects the onset of liver injuries and coordinates the production of a protein pharmaceutical which prevents liver damage.

Thermal regeneration of activated carbon /

Mode of access: Internet.

Judith : or, The regeneration of Manasseh : an oratorio /

Mode of access: Internet.

Results of regeneration cutting in a spruce-subalpine fir stand /

Includes bibliographical references.

Observations on hepatic diseases, incidental to europeans, in the East-Indies /

Mode of access: Internet.

My Mamie Rose; the story of my regeneration,

Mode of access: Internet.

Indian paint fungus : a method for recognizing and reducing hazard in advanced grand and white fir regeneration in eastern Oregon and Washington /

Bibliography: p. 9.

Christian memoirs, or the nature of conviction of sin and regeneration ...

Mode of access: Internet.

Current cancer research on hepatic neoplasms.

Prepared for the ICRDB Program by the Current Cancer Research Project Analyis Center.

Der histologische Vorgang bei der Regeneration der Hufmatrix.

Inaug.-diss.--Hannover, 1912.

Rural regeneration in England; a short discussion of some of the outstanding features of the rural land question and of the principal proposals for reform ....

Mode of access: Internet.

Original sin, free-will, grace, regeneration, justification, faith, good works, and universal redemption : as maintained in certain declarations of our reformers, which are the groundwork of the articles of our established church upon these subjects : with an important account of the subscription to the articles in 1604, and an historical and critical introduction to the whole /

Mode of access: Internet.

Flora hibernica, comprising the flowering plants, ferns, characeæ, musci, hepaticæ, lichenes and algæ of Ireland, arranged according to the natural systems, with a synopsis of the genera according to the Linnæan system.

Mode of access: Internet.

The mysteries opened, or, Scriptural views of preaching and the sacraments, as distinguished from certain theories concerning baptismal regeneration and the real presence /

Mode of access: Internet.