Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle

Background: The model eukaryote, Tetrahymena thermophila, is the first ciliated protozoan whose genome has been sequenced, enabling genome-wide analysis of gene expression. Methodology/Principal Findings: A genome-wide microarray platform containing the predicted coding sequences (putative genes) for T. thermophila is described, validated and used to study gene expression during the three major stages of the organism's life cycle: growth, starvation and conjugation. Conclusions/Significance: Of the,27,000 predicted open reading frames, transcripts homologous to only,5900 are not detectable in any of these life cycle stages, indicating that this single-celled organism does indeed contain a large number of functional genes. Transcripts from over 5000 predicted genes are expressed at levels >5x corrected background and 95 genes are expressed at >250x corrected background in all stages. Transcripts homologous to 91 predicted genes are specifically expressed and 155 more are highly up-regulated in growing cells, while 90 are specifically expressed and 616 are up-regulated during starvation. Strikingly, transcripts homologous to 1068 predicted genes are specifically expressed and 1753 are significantly up-regulated during conjugation. The patterns of gene expression during conjugation correlate well with the developmental stages of meiosis, nuclear differentiation and DNA elimination. The relationship between gene expression and chromosome fragmentation is analyzed. Genes encoding proteins known to interact or to function in complexes show similar expression patterns, indicating that co-ordinate expression with putative genes of known function can identify genes with related functions. New candidate genes associated with the RNAi-like process of DNA elimination and with meiosis are identified and the late stages of conjugation are shown to be characterized by specific expression of an unexpectedly large and diverse number of genes not involved in nuclear functions.

Knotted wave dislocation with the hopf invariant

We study the wave dislocations with an induced gauge potential. The topological current characterized the wave dislocations is constructed with the dual of Abelian gauge field. And the topological charges and locations of the wave dislocations are determined by the phi-mapping topological current theory. Furthermore, it is shown that the knotted wave dislocations can be described with a Hopf invariant in the wave field. At last we discussed the evolution of the knotted wave dislocations.

A Particle-in-cell scheme of the RFQ in the SSC-Linac

A 52 MHz Radio Frequency Quadrupole (RFQ) linear accelerator (linac) is designed to serve as an initial structure for the SSC-Linac system (injector into Separated Sector Cyclotron). The designed injection and output energy are 3.5 keV/u and 143 keV/u, respectively. The beam dynamics in this RFQ have been studied using a three-dimensional Particle-In-Cell (PIC) code BEAMPATH. Simulation results show that this R,FQ structure is characterized by stable values of beam transmission efficiency (at least 95%) for both zero-current mode and the space charge dominated regime. The beam accelerated in the RFQ has good quality in both transverse and longitudinal directions, and could easily be accepted by Drift Tube Linac (DTL). The effect of the vane error and that of the space charge on the beam parameters have been studied as well to define the engineering tolerance for RFQ vane machining and alignment.

Activated carbon supported bimetallic CoMo carbides synthesized by carbothermal hydrogen reduction

A carbothermal hydrogen reduction method was employed for the preparation of activated carbon supported bimetallic carbide. The resultant samples were characterized by BET surface area measurement, X-ray diffraction, and temperature-programmed reduction-mass spectroscopy. The results showed that nanostructured beta-Mo2C can be formed on the activated carbon by carbothermal hydrogen reduction above 700 degreesC. The particle sizes of beta-Mo2C increase with increasing reaction temperatures and Mo loading. The bimetallic CoMo carbide can be synthesized by the carbothermal hydrogen reduction even around 600 degreesC. The bimetallic CoMo carbide is from carbothermal hydrogen reduction of CoMoO4 precursor and is easily formed when the Co/Mo molar ratio is 1.0. Separation of the bimetallic CoMo carbide phase into Mo carbide and Co metal occurs when the temperature of the reduction is above 700 degreesC. The addition of a second metal such as Co and Ni, decreases the formation temperature of carbide because the second metal promotes formation of CHx species from reactive carbon atoms or groups on carbon material and hydrogen, which further carburizes oxide precursors. (C) 2003 Elsevier Science Ltd. All rights reserved.

化肥防结块剂的类型及应用

阐述肥料结块的原因及影响因素,介绍防结块剂的类型和效果,并提出防结块剂应用的现存问题和发展前景。

鱇白鱼的池塘人工驯养方法

本发明涉及一种鱇白鱼的池塘人工驯养方法，属鱼产品养殖技术领域。鱇白鱼的池塘人工驯养方法为先用消毒剂对池塘进行清塘，随后放入清水，其水深为0.8米，此时将鱼苗放入池中，驯养期间水温在8-25℃，鱼苗投喂时间为每日二次，投喂的饲料分别为：一个月前喂蛋黄或轮虫，随后喂蛋黄、豆浆和混合饲料，5个月后投喂混合饲料，饲料的喂量为蛋黄每次5个，豆浆每天0.5公斤黄豆研磨成浆，混合饲料早期每次1公斤，随着鱼体长大酌情增量。采用本鱇白鱼的池塘人工驯养方法，具有操作简便，成本低廉，鱼苗成活率高，并将在自然状况下，生长到5厘米左右时便进入40-50米的深水区生长发育，直到性成熟后才洄游到浅水区的砾石砂滩和溶洞出水口处产卵的抚仙湖特有鱼类——鱇白鱼驯化成为一种池塘人工养殖品种等优点。