Qualidade fisiológica de sementes de Sebastiania commersoniana (Baill.) Smith & Downs (branquilho - Euphorbiaceae) durante o armazenamento

This paper aims to evaluate the physiological quality of Sebastiania commersoniana seeds stored in cloth bag, plastic bag and glass container and kept in two conditions: laboratory workbench and cold chamber (4 ± 2°C e 80% relative humidity - RH) for 531 days. Periodically, samples were taken in order to determine the moisture content and to perform germination tests. The experimental design was completely randomized, in factorial scheme of 3 × 2 (wrappings × conditions), for each period of storage evaluated (0, 158, 271, 389 and 531 days of storage). Supplementary, a polynomial regression was adjusted in order to describe the physiological quality of the seeds for the different conditions during the experimental period. The results lead to the following considerations: a) storage under variable temperature and air humidity (laboratory workbench) can be used for periods of over five and a half months, with the seeds stored in cloth bag, plastic bag or glass container; b) the physiological quality of the seeds was not hardly altered when stored in cold chamber in cloth bag, plastic bag and glass container for over 18 months.

Testes de vigor para avaliação da qualidade fisiológica de sementesde Sebastiania commersoniana (Baill.) Smith & Downs.

The objective of this work was to evaluate the efficiency of different vigor tests to assess S. commersoniana seeds physiological quality during storage. Therefore, seeds were stored in cloth bags, plastic bags and glass containers for 531 days, both at room temperature and in a cold chamber. Periodically samples were taken and the following tests were conducted: standard germination, germination speed index, first count after germination, seedlings fresh and dry matter, electrical conductivity (imbibitions for 2 and 24 hours) and accelerated aging, in order to monitoring the seeds physiologic quality. Data were submitted to polynomial regression analysis, for each of the combination of packaging/storage condition. The results obtained led to the following considerations: a) the speed germination index and the accelerated aging test permitted a good evaluation of the seed-lots quality; b) the first count of germination did not allow any prediction about seed lots vigor; c) seedling fresh and dry matter did not discriminate among seed lots; d) electrical conductivity test after 2 h imbibitions was not adequate to evaluate the seed lots vigor and e) the electric conductivity test with 24 h imbibitions was more adequate to differentiate among seed lots, but with little efficiency to discriminate among seed lots with intermediate vigor or with low quality differences.

Regeneração natural de espécies da Mata Atlǎntica em sub-bosque de Eucalyptus saligna Smith. em uma antiga unidade de produção forestal no Parque das Neblinas, Bertioga, SP

This study aimed at characterizing the potential for natural regeneration of native vegetation in the under-story of an earlier Eucalyptus saligna Smith production stand. The study was carried out at the Parque das Neblinas, Bertioga municipality, SP, in a 45 ha third rotation stand; which had been abandoned 15 years ago for natural regeneration to occur. The sampling was done in 24 plots of 20 × 40 m. The sampled area was of 19,200 m2, with inventory made of 100% of the eucalyptus trees. All regeneration trees with a height ≥ 1.30 m and DBH ≥ 5.0 cm were measured, as well as adult individuals with DBH ≥ 5.0 cm; surveyed in two size classes. 1,417 individuals of E. saligna were measured, with a density of 738,02 individuals/ha and a basal area of 22.69 m2/ha. Among 2,763 natural regeneration individuals, 111 species belonged to 66 genera and 34 botanical families. The species represented 43.7% of the tree richness of neighboring native forest fragments. The total estimated density and the basal area were respectively 1,052.6 individuals/ha and 6.4 m2/ha of autochthonous trees with DBH ≥ 5.0 cm (Class 1); while for regeneration there were 3,864.58 individuals/ha, and 2.76 m2/ha of individuals with a height ≥ 1.30 m and DBH < 5.0 cm (Class 2). Shannon diversity (H') was 2.83 and 3.68, respectively, for Classes 1 and 2, and the corrected species richness for a 1000-individual sample (R1000) were 75.6 and 87.29 (Fisher's a index) for the same classes. The majority of the species (34.84%) was typical from the understory of wet tropical forest and had zoochoric fruit dispersal (67.57%). The results indicate that, under these conditions, a eucalyptus forest is able to provide adequate regeneration niches for native vegetation, and may represent a sink habitat for local populations.

Detection of classical 17p11.2 deletions, an atypical deletion and RAI1 alterations in patients with features suggestive of Smith-Magenis syndrome

Smith-Magenis syndrome (SMS) is a complex disorder whose clinical features include mild to severe intellectual disability with speech delay, growth failure, brachycephaly, flat midface, short broad hands, and behavioral problems. SMS is typically caused by a large deletion on 17p11.2 that encompasses multiple genes including the retinoic acid induced 1, RAI1, gene or a mutation in the RAI1 gene. Here we have evaluated 30 patients with suspected SMS and identified SMS-associated classical 17p11.2 deletions in six patients, an atypical deletion of ∼139 kb that partially deletes the RAI1 gene in one patient, and RAI1 gene nonsynonymous alterations of unknown significance in two unrelated patients. The RAI1 mutant proteins showed no significant alterations in molecular weight, subcellular localization and transcriptional activity. Clinical features of patients with or without 17p11.2 deletions and mutations involving the RAI1 gene were compared to identify phenotypes that may be useful in diagnosing patients with SMS. © 2012 Macmillan Publishers Limited All rights reserved.

Corn yield with reduction of insecticidal sprayings against fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae)

The ideal time is more important than amount of insecticidal spraying to adequate the control of Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) in corn. This study aimed to evaluate lufenuron sequential sprayings effect and its rotation with other active ingredients on the population, damage caused by S. frugiperda and the impact on corn yield. The experiment was carried out in the field with six treatments: (1) one lufenuron spraying, (2) two lufenuron sprayings, (3) three lufenuron sprayings (4) four lufenuron sprayings, (5) sprayings with spinosad, lufenuron, thiamethoxam+lambdacyhalothrin and deltamethrin (in sequence, at ten days intervals) (6) control treatment. Sprayings started twenty days after the seedling had emerged and then every ten days for a maximum of four sprays. Both caterpillar population (20.9-21.7 larvae/plot) and index of damage (1.2-1.7) observed in corn plants were significantly lower in treated plots compared to control (untreated) (31.7 larvae/plot and index of damage 2.7), regardless of spraying amount. The results showed that multiple insecticide applications to control S. frugiperda do not guarantee higher yields in corn, ranging from 6375.2 to 7650.1 kg ha -1. Only one spraying of lufenuron was enough to prevent significant reduction in corn yield (6749.9 kg ha -1). © 2012 Asian Network for Scientific Information.

Division of labor in pachycondyla striata Fr. Smith, 1858 (Hymenoptera: Formicidae: Ponerinae)

Four colonies of the ant Pachycondyla striata were used to analyze the specie behavioral repertoire. Forty-six behavioral acts were recorded in laboratory. Here, we present the record the division of labor between the castes and the temporal polyethism of monomorphic workers. The queens carried out many of the behavioral traits recorded in this work however; they performed them less frequently compared to the worker. The workers activity involved chasing and feeding on fresh insects and usingthem to nourish larvae besides laying eggs in the C-posture, an activity also performed by queens, which is similar to that of wasps of the subfamily Stenogastrinae. The young workers were involved in activities of brood care, sexuate care, and nest maintenance, and the older workers were involved in defense, exploration, and foraging. © 2012 Adolfo da Silva-Melo and Edilberto Giannotti.

Intraspecific and intracolonial variation in the profile of venom alkaloids and cuticular hydrocarbons of the fire ant Solenopsis saevissima smith (Hymenoptera: Formicidae)

Fire ants are aggressive Neotropical ants that are extensively similar in general biology and morphology, making species identification difficult. Some fire ant species are top-rated pests spreading throughout the world by trade vessels. Many researchers attempted to sort between invasive and native species by using chemical characters, including patterns of venom alkaloids. The present study is the first to report intraspecific variation in some chemical characters, namely, cuticular hydrocarbons and venom alkaloids, within the Brazilian fire ant species Solenopsis saevissima and also reports on within-nest variations among members of different castes. Two different haplotypes (cryptic species) of S. saevissima were clearly identified, one presenting a predominant combination of the venom alkaloids cis- and trans-2-methyl-6-undecylpiperidine with the cuticular hydrocarbons C23, 3-Me-C23, 10-C 25:1, C25, and 3-Me-C25, and the other a predominant combination of cis- and trans-2-methyl-6-tridecenylpiperidine with predominance of 12-C25:1, C25, 11-Me-C25, 3-Me-C25, 13-C27:1, C27, and 13-Me-C 27. Intranest variations revealed that the proportions among these compounds varied sensibly among workers of different sizes, gynes, and males (no alkaloids were detected in the latter). Larva contained vestiges of the same compounds. The recorded chemical profiles are quite different from previous reports with S. saevissima samples from So Paulo. The finds thus support other recent claims that S. saevissima includes cryptic species; the study, moreover, adds the find that they can occur in the same geographical location. © 2012 Eduardo Gonalves Paterson Fox et al.

Osteologia craniana de Micrastur semitorquatus Vieillot, 1817 (Falconiformes: Falconidae)

Micrastur semitorquatus, popularly known as Collared Forest-Falcon is an inhabitant of the rainforests of the Neotropical Region. Studies on the cranial osteology are scarce in the literature. To increase the knowledge about the anatomy of this species its cranial osteology was described and compared with other species of birds of prey. Three specimens of M. semitorquatus were examined at the Bird Division of the osteological collection at the Smithsonian National Museum of Natural History (USNM), Washington, DC (USNM: 245788, USNM 013493 and USNM 289773). Although the relationship between the form of a structure and its function is not fully established, many of the adaptations of the skull of M. semitorquatus may be related to this lifestyle and behavior, such as narrowing of the interorbital width, the robustness of pila supranasalis coupled with the prokinesis of the skull, a process developed post-orbital, and palatine expansion. In addition, the cranial osteology of M. semitorquatus can provide useful characters for cladistic analysis, sustaining the hypothesis of phylogenetic relationships with other species of the same genus or the same family.

La población y el desarrollo en el pensamiento de A. Smith y R.T. Malthus

Documento Docente - Programa Global de Formación en Población y Desarrollo

Histochemical study of the hepatopancreas in adult females of the pink-shrimp Farfantepenaeus brasiliensis Latreille, 1817

This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads). The F. brasiliensis females were collected in seawater off the Guarapari coast, Espirito Santo, Brazil. Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive). The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids). Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli. In females with exhausted gonads polysaccharides were also seen in the intertubular spaces and inside the lumina of the tubules. The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells. The bound calcium was mainly found in the F and M cells. The F cells showed strong positive staining for lipid while the R cell only stained weakly. The E cells did not react to any of the applied staining techniques. The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis. Also, they may be more related to the molt stage, as in the case of calcium salts. © 2013 Elsevier GmbH. All rights reserved.

Estudos estruturais e funcionais das hemoglobinas de Phrynops geoffroanus (Schweigger, 1812)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Padrão de atividades, ecologia alimentar e área de vida em um grupo de Callithrix penicillata (Humboldt, 1812)

Pós-graduação em Biologia Animal - IBILCE

Sistemática molecular de Atta laevigata (Smith 1858) e Acromyrmex balzani (Emery 1890)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aspectos ultrassonográficos, biometria e dopplerfluxometria ocular de primatas-Alouatta fusca (BUGIO RUIVO-Geoffroy Saint-Hilaire, 1812)

Pós-graduação em Medicina Veterinária - FMVZ