Ocean Acidification Affects Redox-Balance and Ion-Homeostasis in the Life-Cycle Stages of Emiliania huxleyi

Ocean Acidification (OA) has been shown to affect photosynthesis and calcification in the coccolithophore Emiliania huxleyi, a cosmopolitan calcifier that significantly contributes to the regulation of the biological carbon pumps. Its non-calcifying, haploid life-cycle stage was found to be relatively unaffected by OA with respect to biomass production. Deeper insights into physiological key processes and their dependence on environmental factors are lacking, but are required to understand and possibly estimate the dynamics of carbon cycling in present and future oceans. Therefore, calcifying diploid and non-calcifying haploid cells were acclimated to present and future CO2 partial pressures (pCO2; 38.5 Pa vs. 101.3 Pa CO2) under low and high light (50 vs. 300 µmol photons/m**2 /s). Comparative microarray-based transcriptome profiling was used to screen for the underlying cellular processes and allowed to follow up interpretations derived from physiological data. In the diplont, the observed increases in biomass production under OA are likely caused by stimulated production of glycoconjugates and lipids. The observed lowered calcification under OA can be attributed to impaired signal-transduction and ion-transport. The haplont utilizes distinct genes and metabolic pathways, reflecting the stage-specific usage of certain portions of the genome. With respect to functionality and energy-dependence, however, the transcriptomic OA-responses resemble those of the diplont. In both life-cycle stages, OA affects the cellular redox-state as a master regulator and thereby causes a metabolic shift from oxidative towards reductive pathways, which involves a reconstellation of carbon flux networks within and across compartments. Whereas signal transduction and ion-homeostasis appear equally OA-sensitive under both light intensities, the effects on carbon metabolism and light physiology are clearly modulated by light availability. These interactive effects can be attributed to the influence of OA and light on the redox equilibria of NAD and NADP, which function as major sensors for energization and stress. This generic mode of action of OA may therefore provoke similar cell-physiological responses in other protists.

(Table 1) Stratigraphic datums from ODP Site 130-806

A 2 m.y. oxygen isotope record of Globigerinoides sacculifer from the Ontong Java Plateau, based on cores from Ocean Drilling Program Leg 130, is dated by matching variations to an orbital template. The procedure allows us to present the most complete Quaternary record available for the western equatorial Pacific. The template-generating algorithm describes a balance between growth and melting of ice. Following basic Milankovitch theory, ice growth is taken as constant, while melting is taken to depend on summer insolation, current ice mass, and average past ice mass. Template settings must be changed once, between 1 and 1.2 Ma, to reflect a major shift in climate. Template fits are strikingly good over much of the record and can be used to detect and fill gaps from core breaks and other disturbances. One result of template dating is an exact age for the Brunhes-Matuyama magnetic reversal boundary, at 790+/-5 ka, as well as several other precise dates (900 ka for the middle Pleistocene climate shift; 1070, 1240, and 1450 ka for isotope stages 31, 37, and 47, respectively). Sedimentation rates fluctuate between 18 and 28 m/m.y., a ca. 400 ka cycle being the most prominent. Major anomalies arise within the transitional regime (1.2 to 1 Ma). The origin of the cycles is unknown; we propose productivity variations in the western equatorial Pacific.