Caracteriza����o das prote��nas caveolinas -1 e -2 na placenta de conceptos bovinos clonados transg��nicos

The transgenic application of green fluorescent protein (GFP) as fetal cell marker on cattle cloned placenta could provide an exclusive model for studying the morphologic and immunologic maternal-fetal interactions, providing information about its mapping, distinguishing the fetal from maternal cells. This model will have direct application, mainly because these animals present problems during its development. With this model's support, we intend to verify the substances transport between mother and fetus during endocytosis, through the immunolocalization of protein named caveolae. For these, we used 06 cloned bovine and 30 cattle samples of artificial insemination (AI) with 90 days of pregnancy, which had been their development interrupted by humanitarian slaughter of the recipient and recovery of the pregnant uterus. We collected the placentome and the chorion. A part of the samples was cut and fixed, by immersion, on a solution containing 4% of parafomaldehyde or 10% of formaldehyde on a sodium phosphate buffer (PBS), at 0,1 M pH 7.4, Zamboni solution (4% of paraformaldehyde, 15% of picric acid, on sodium phosphate buffer 0,1 M pH 7.4), metacarn (60% of metanol, 30% of chloroform, and 10% glacial acetic acid), for morphologic and immunohistochemistry verification for caveolinas proteins -1 and -2 (CAV -1 and CAV-2). The caveolins -1 were found in fetal and maternal villi, but its strongest staining was observed in the endometrial stroma. The caveolins -2 had positive staining in trophoblast and chorioallantoic membrane, and specifically in giant trophoblastic binucleated cell. Therefore the results were compared between cloned cattle and from AI or natural mating, for assisting on detection of the reason of many placental alterations, embryonic losses, spontaneous abortion, post-natal mortality and large offspring syndrome on laboratory-manipulated animals. The result suggests that the proteins caveolins -1 and -2 (CAV-1 and CAV-2) are part of the caveolae composition and important structures related to the molecule transfer to the fetus, nourish it through endocytosis and pinocytosis.

Propiedades termof��sicas del jugo concentrado de lulo a temperaturas por encima del punto de congelaci��n

Neste trabalho, foram determinados experimentalmente o calor espec��fico, a condutividade t��rmica, difusividade t��rmica e densidade de suco de lulo na faixa de conte��do de ��gua entre 0,55 e 0,90 (p/p em base ��mida) e em temperaturas entre 4 e 78,6 ��C. A condutividade t��rmica e o calor espec��fico foram obtidos utilizando-se o mesmo equipamento - uma célula constitu��da de dois cilindros conc��ntricos - operando em estado estacion��rio e n��o estacion��rio, respectivamente. A difusividade t��rmica foi obtida atrav��s do m��todo de Dickerson e a densidade determinada por picnometria. Tanto a temperatura como o conte��do de ��gua apresentaram grande influ��ncia nos dados experimentais das propriedades termof��sicas do suco de lulo. Os resultados obtidos foram utilizados na obten����o de modelos matem��ticos para predizer estas propriedades em fun����o da concentra����o e temperatura.

Study on the origin and nature of the adenomatoid odontogenic tumor by immunohistochemistry

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Migra����o e invas��o celular in vitro de células humanas expressando variantes da oncoprote��na LMP1 do v��rus de Epstein-Barr (EBV)

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Transcri����o cooperativa de genes ribossomais em Escherichia coli usando um modelo estoc��stico e dependente de sequ��ncia

Conselho Nacional de Desenvolvimento Cient��fico e Tecnol��gico (CNPq)

Funcionalidade das prote��nas EF-Tu e 14-3-3 na virul��ncia de Paracoccidioides brasiliensis

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Avalia����o laboratorial da cinomose canina: estudo retrospectivo de 25 casos no munic��pio de Ara��atuba-SP

Canine Distemper is a contagious, severe and multisystemic disease caused by a virus from Morbilliv��rus genus. The virus is distributed worldwide and it presents a high lethality rate, affecting mainly dogs. The diagnosis is based on clinical signs associated with hematological fi ndings. The observation of Lentz bodies in erythrocytes and leukocytes is the defi nitive diagnosis for the disease. The aim of this work was to test the hypothesis that the hematological profi le in dogs positive for canine distemper differs according to blood cell type presenting Lentz bodies. For this purpose, 25 dogs positive for the disease were evaluated at the Veterinary Hospital ���Lu��s Quintiliano de Oliveira��� UNESP, Ara��atuba city. The diagnosis was based on the observation of Lentz bodies in blood smears. Fromthe total, 64% of dogs presented anemia, 16% leucopenia and 12% leukocytosis. Lymphopenia occurred in 76% of dogs. Viral inclusions were observed solely in neutrophils (32%), lymphocytes (28%) and erythrocytes (12%). Concomitant observation occurred in lymphocytes and erythrocytes (4 %), in lymphocytes and neutrophils (12%), in neutrophils and monocytes (4%) and in neutrophils and erythrocytes (4%). In an isolated case Lentz bodies were observed simultaneously in neuthrophils, monocytes and lymphocytes. In conclusion, hematological profi le is not associated with the presence of viral inclusion in a particular cell type.

Vacina de DNA: uma alternativa para o tratamento do melanoma canino: revis��o de literatura

O melanoma �� considerado um dos tumores cut��neos de maior import��ncia em c��es devido ao seu car��ter agressivo, �� capacidade de produzir met��stases em est��gios precoces e �� baixa resposta aos tratamentos n��o cir��rgicos. Recentemente, as vacinas de DNA apresentam-se promissoras na terap��utica do melanoma canino. O objetivo deste trabalho �� apresentar uma revis��o bibliogr��fica sobre o assunto. As vacinas de DNA s��o baseadas em plasm��deos que cont��m o gene codificante para o ant��geno alvo, expressando-o na célula do hospedeiro e apresentando, assim, vantagens em rela����o ��s vacinas tradicionais, como: facilidade de produ����o, estabilidade t��rmica, baixo custo e estimula����o da resposta imune celular (linf��citos T CD8+). Devido ao sucesso limitado das terapias padr��es, o estabelecimento de uma vacina de DNA efetiva aumenta a possibilidade de uma terapia promissora para o melanoma canino, podendo trazer novas expectativas aos animais portadores dessa neoplasia.

Corrosion resistance of Ni-Cr alloys in different mouthwashes

Several alloys have been used for prosthodontics restorations in the last years. These alloys have a number of metals that include gold, palladium, silver, nickel, cobalt, chromium and titanium and they are used in oral cavity undergo several corrosion. Corrosion can lead to poor esthetics, compromise of physical properties, or increased biological irritation. The objective of this study was evaluated corrosion resistance of two alloys Ni-Cr and Ni-Cr-Ti in three types of mouthwashes with different active ingredients: 0.5g/l cetylpyridinium chloride + 0.05% sodium fluoride, 0.05% sodium fluoride + 0.03% triclosan (with fluor) and 0.12% chlorohexidine digluconate. The potentiodynamic curves were performed by means of an EG&G PAR 283 potentiostat/galvanostat. The counter electrode was a platinum wire and reference electrode was an Ag/AgCl, KCl saturated. Before each experiment, working electrodes were mechanically polished with 600 and 1200 grade papers, rinsed with distilled water and dried in air. All experiments were carried out at 37.0oC in conventional three-compartment double wall glass cell containing mouthwashes. The microstructures of two alloys were observed in optical microscopy. Analysis of curves showed that Ni-Cr alloy was less reactive in the presence of 0.12% chlorohexidine digluconate while Ni-Cr-Ti alloy was more sensitive for others two types of mouthwashes (0.5g/l cetylpyridinium chloride + 0.05% sodium fluoride ��� and 0.05% sodium fluoride + 0.03% triclosan). This occurred probably due presence of titanium in this alloy. Microstructural analysis reveals the presence of dendritic and eutectic microstructures for NiCr and Ni-Cr-Ti, respectively.

Avalia����o da resist��ncia adesiva de diferentes sistemas resinosos de colagem ortod��ntica

Recent orthodontic bonding materials have aimed to reduce the working time of the clinician, by simplifying the acid etching procedure by applying self-etching primer adhesive systems. However, the adhesion quality of these materials still demands investigation. Thus, the present study aimed to evaluate the bond strengths of three different adhesive systems. A hundred and eighty bovine lower incisors were cut and embebbed in acrylic resin matrices, in which orthodontic brackets were bonded with Transbond XT (2n = 60), Transbond XT Self Etching Primer (2n = 60) e Tyrian (2n = 60). For each composite, bond strength tests were executed immediately (n = 30) and 24 hours (n = 30) after the bonding, in the assay machine Versat 2000 (Pantec), by applying 500 Kgf of load at 1mm/min of velocity. Transbond XT, Transbond XT Self Etching Primer and Tyrian presented, respectively, average values of bond strength of 7.43, 7.09 and 3.41 MPa at the time immediately, and 7.42, 8.81 and 5.35 MPa at 24 hours after the bonding, where differences were statistically significant at 5% between Tyrian and Transbond groups at both observation times. It was concluded that Tyrian was the material that presented significant lower bond strength with regard to Transbond groups that were similar.

Terapia fotodin��mica na Endodontia: emprego de uma estrat��gia coadjuvante frente �� infec����o endod��ntica

O tratamento endod��ntico �� de fundamental import��ncia para abolir a infec����o nos dentes com necrose pulpar. O sucesso desse tratamento depende: da elimina����o eficiente da infec����o no sistema de canais radiculares (SCR) e do correto selamento pela obtura����o dos canais radiculares. Devido �� complexidade anat��mica do SCR, certas ��reas podem ser inacess��veis ao preparo biomec��nico (PBM), portanto, o emprego de uma medica����o intracanal potencializa a redu����o dos microrganismos (MO) e seus produtos t��xicos no SCR. Mesmo com o avan��o t��cnico e cient��fico da Endodontia, h�� MO que ainda sobrevivem ao PBM, sendo os principais respons��veis pela manuten����o infec����o endod��ntica. Assim, novos tratamentos devem ser pesquisados. Com o advento dos aparelhos de laser e LED, surgiram alternativas de tratamentos na ��rea da sa��de, como a terapia fotodin��mica (TFD), que �� um conjunto de procedimentos f��sicos, qu��micos e biol��gicos, que ocorrem ap��s a administra����o de um agente fotossensibilizador (FS) ativado por meio de uma luz vis��vel de comprimento de onda espec��fico (laser ou LED) para destruir a célula-alvo, ou auxiliar no combate das infec����es. Na Endodontia, foram demonstrados em estudos in vitro e in vivo que o emprego da TFD atua como um coadjuvante e potencializa a desinfec����o do SCR, al��m de ser de f��cil aplica����o e n��o promover resist��ncia microbiana. O objetivo da presente revis��o �� apresentar o estado atual da terapia fotodin��mica em Endodontia.

Sistem��tica e filogenia do fungo parasita Escovopsis associado ��s formigas Attini

Conselho Nacional de Desenvolvimento Cient��fico e Tecnol��gico (CNPq)

An��lise da cin��tica do VO2 no dom��nio severo do crawl em condi����o atada de nado

P��s-gradua����o em Desenvolvimento Humano e Tecnologias - IBRC

Influ��ncia das células mioepiteliais neopl��sicas benignas de adenoma pleom��rfico na produ����o de MMPs -1, -2, - 8, -9 e -13 por células epiteliais malignas e sua poss��vel rela����o com o ��ndice de invas��o

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Quantifica����o de citocinas no conte��do abomasal de bovinos de corte na presen��a ou aus��ncia de ulcera����o g��strica

P��s-gradua����o em Ci��ncia Animal - FMVA