Genetic maps of the proximal half of chromosome arm 2L of Drosophila melanogaster

Drosophila mutants have played an important role in elucidating the physiologic function of genes. Large-scale projects have succeeded in producing mutations in a large proportion of Drosophila genes. Many mutant fly lines have also been produced through the efforts of individual laboratories over the past century. In an effort to make some of these mutants more useful to the research community, we systematically mapped a large number of mutations affecting genes in the proximal half of chromosome arm 2L to more precisely defined regions, defined by deficiency intervals, and, when possible, by individual complementation groups. To further analyze regions 36 and 39-40, we produced 11 new deficiencies with gamma irradiation, and we constructed 6 new deficiencies in region 30-33, using the DrosDel system. trans-heterozygous combinations of deficiencies revealed 5 additional functions, essential for viability or fertility.

Simulating dioxane transport in a heterogeneous glacial aquifer system (Washtenaw County, Michigan) using publicly available models and data

The primary challenge in groundwater and contaminant transport modeling is obtaining the data needed for constructing, calibrating and testing the models. Large amounts of data are necessary for describing the hydrostratigraphy in areas with complex geology. Increasingly states are making spatial data available that can be used for input to groundwater flow models. The appropriateness of this data for large-scale flow systems has not been tested. This study focuses on modeling a plume of 1,4-dioxane in a heterogeneous aquifer system in Scio Township, Washtenaw County, Michigan. The analysis consisted of: (1) characterization of hydrogeology of the area and construction of a conceptual model based on publicly available spatial data, (2) development and calibration of a regional flow model for the site, (3) conversion of the regional model to a more highly resolved local model, (4) simulation of the dioxane plume, and (5) evaluation of the model's ability to simulate field data and estimation of the possible dioxane sources and subsequent migration until maximum concentrations are at or below the Michigan Department of Environmental Quality's residential cleanup standard for groundwater (85 ppb). MODFLOW-2000 and MT3D programs were utilized to simulate the groundwater flow and the development and movement of the 1, 4-dioxane plume, respectively. MODFLOW simulates transient groundwater flow in a quasi-3-dimensional sense, subject to a variety of boundary conditions that can simulate recharge, pumping, and surface-/groundwater interactions. MT3D simulates solute advection with groundwater flow (using the flow solution from MODFLOW), dispersion, source/sink mixing, and chemical reaction of contaminants. This modeling approach was successful at simulating the groundwater flows by calibrating recharge and hydraulic conductivities. The plume transport was adequately simulated using literature dispersivity and sorption coefficients, although the plume geometries were not well constrained.

UPLC-ESI-TOFMS-based metabolomics and gene expression dynamics inspector self-organizing metabolomic maps as tools for understanding the cellular response to ionizing radiation

Global transcriptomic and proteomic profiling platforms have yielded important insights into the complex response to ionizing radiation (IR). Nonetheless, little is known about the ways in which small cellular metabolite concentrations change in response to IR. Here, a metabolomics approach using ultraperformance liquid chromatography coupled with electrospray time-of-flight mass spectrometry was used to profile, over time, the hydrophilic metabolome of TK6 cells exposed to IR doses ranging from 0.5 to 8.0 Gy. Multivariate data analysis of the positive ions revealed dose- and time-dependent clustering of the irradiated cells and identified certain constituents of the water-soluble metabolome as being significantly depleted as early as 1 h after IR. Tandem mass spectrometry was used to confirm metabolite identity. Many of the depleted metabolites are associated with oxidative stress and DNA repair pathways. Included are reduced glutathione, adenosine monophosphate, nicotinamide adenine dinucleotide, and spermine. Similar measurements were performed with a transformed fibroblast cell line, BJ, and it was found that a subset of the identified TK6 metabolites were effective in IR dose discrimination. The GEDI (Gene Expression Dynamics Inspector) algorithm, which is based on self-organizing maps, was used to visualize dynamic global changes in the TK6 metabolome that resulted from IR. It revealed dose-dependent clustering of ions sharing the same trends in concentration change across radiation doses. "Radiation metabolomics," the application of metabolomic analysis to the field of radiobiology, promises to increase our understanding of cellular responses to stressors such as radiation.

Auriferous Tertiary Gravels Near Rocker, in Silver Bow County, Montana

Between the villages of Rocker and Silver Bow, in south­western Montana, are found an interesting group of placers. Gold occurs in Tertiary gravel beds that are interstratified with beds of rhyolitic volcanic ash. With the aid of a plane table and open-sight alidade, a small portion of the lake-bed area near Rocker was mapped; all distances were paced, but numerous checks assure a fairly accurate map.

Geology and Ore Deposits of the Golden Messenger MIne, Lewis and Clark County, Helena, Montana.

The Golden Messenger Mine which is approximately twenty-three miles northeast of Helena, Montana, near York, on Trout Creek, has long presented several problems of both theoretical and practical interest.

Effects of Enhanced Oil Recovery on Production Engineers, Elm Coulee Field, Bakken Reservoir, Richland County, Montana

This presentation describes research at Montana Tech to improve oil recovery rates in the Elm Coulee Oil Field in the Bakken Reservoir, Richmond County, Montana. The slides display current recovery rate predictions, enhanced oil recovery methods, reservoir model building and history matching strategies. Recommended development strategies include implementing hydrocarbon gas injection operations to improve current oil recovery rates by more than 75%. The impact of increased production on Production Engineers is also described. The Principal Investigators include John Evans, Leo Heath, David Reichhardt and Burt Todd in the Petroleum Engineering Department.

Geology of a Portion of the Bull Mountain Range Jefferson County, Montana

Work was first done on a known section, the south Boulder Section, in order to familiarize the student with the formations. Most of the area was mapped by plane table and telescopic alidade, general features being surveyed by automobile traverse and a pacing traverse.

Report on the Ermont Mine Sec. 35, T. 6 S., R. 11 W., Beaverhead County, Montana

An examination of the Ermont Mine was requested by the owners, Messrs. J. R. Bowles and R. B. Caswell, to determine the amount and grade of ore developed, the advisability of constructing a mill at the present time, and to recommend future development work.

Some Chromite Deposits in Madison County, Montana

Chromite occurs in Madison County, Montana, in two areas, one three miles southwest of Silver Star, the other five miles southeast of Sheridan. The ore bodies are small, lenticular masses surrounded by metamorphosed sedimentary rocks of the pre-Cambrian Cherry Creek series. The ore was deposited from the hydrothermal solutions which serpentinized the surrounding metamorphic host rocks.

A STUDY OF THE IMPACTS OF FREEZE-THAW ON CLIFF RECESSION AT THE CALVERT CLIFFS IN CALVERT COUNTY, MARYLAND

The Calvert Cliffs, which form much of the western coastline of the Chesapeake Bay in Calvert County, Maryland, are actively eroding and destabilizing, resulting in a critical situation for many homes in close proximity to the slope's crest. Past studies have identified that where waves directly interact with the toe of the slope, wave action controls cliff recession; however, where waves do not regularly interact with the slope toe, the past work identified that freeze-thaw controls recession. This study investigated the validity of this second claim by analyzing the recession rate and freeze-thaw behavior of six study sites along the Calvert Cliffs that are not directly affected by waves. While waves do remove failed material from the toe, in these regions freeze-thaw is believed to be the dominant factor driving recession at the Calvert Cliffs. Past recession rates were calculated using historical aerial photographs and were analyzed together with a number of other variables selected to represent the freeze-thaw behavior of the Calvert Cliffs. The investigation studied sixteen independent variables and found that over 65% of recession at these study sites can be represented by the following five variables: (1) cliff face direction, (2 and 3) the percent of total cliff height composed of soil with freeze-thaw susceptibility F4 and F2, (4) the number of freeze-thaw cycles, and (5) the weighted shear strength. Future mitigation techniques at these sites should focus on addressing these variables and might include vegetation or addressing the presence of water along the face of the slope. Unmitigated, the Calvert Cliffs will continue to recede until a stable slope angle is reached and maintained.

Subsurface to Surface Correlation of the Tensleep Sandstone in the West Flank of the Pryor Mountains in Carbon County, Montana

The Pennsylvanian Tensleep Sandstone is an eolian and nearshore marine/sabka quartz arenite unit with prominent outcrops along the western Pryor/Bighorn Mountain front east of Red Lodge, MT. Regionally, the formation represents one of the largest ergs in the global geologic record. High permeability makes it an important oil and gas reservoir and aquifer in south central Montana and throughout much of Wyoming. The Tensleep Sandstone’s high percentage of quartz content and grain roundness, due to its eolian origin, makes it a prospective source for natural proppant sand. Three continuous 4-inch cores were obtained during a cooperative project between Montana Tech and industry partners. Using stratigraphic sections, cores, thin sections, and x-ray fluorescence (XRF) analysis, the usefulness and economic feasibility of the Tensleep Sandstone as a minable hydraulic fracture proppant was explored. Usefulness depends on cementation, grain shape, grain size, and depth from surface of the prospective zone. Grain shape and size were determined by thin sections, sieving, and stereomicroscope analysis. Analysis of 20 disaggregated sand samples has shown that as much as 30 percent of the grain sizes fall between 30-50 mesh (medium- to finegrained sand size) and about 45 percent of the grain sizes fall between 70–140 mesh (very fine-grained sand to coarse silt), grain sizes appropriate for some hydraulic fracture operations. Core descriptions and XRF data display the distribution of lithology and cementation. Core descriptions and XRF data display the distribution of lithology and cementation. Elemental (XRF) analyses help to delineate more pure quartz sands from those with grain fractions reflecting fine-grained clastic and evaporitic inputs. The core and nearby stratigraphic sections are used to quantify the amount of overburden and the 3 amount of resource in the area. Initial results show favorable crush strength and useable grain size and shape.

Vermiculite, Occurence, Character, and Concentration of Mineral from the Pony District, Madison County, Montana

The purpose of this paper is to investigate the occurrence and character of the vermiculite deposits approximately four miles northwest of Pony; Madison County, Montana. The deposits are situated in rolling foothills at the northern end of the Tobacco Root Mountains.

Isopach Maps and Discussion of the Ordovician and Devonian of Montana and Adjacent Areas

The intensive postwar search for new petroleum horizons has resulted in widespread prospecting in the northern Great Plains. No commercial production has as yet been derived from Ordovician or Devonian rocks in Montana, but the relat­ively few tests that have penetrated to critical depths have disclosed encouraging conditions which merit further consider­ation, especially in Devonian strata.

Geology of Upper Bear Gulch and Dry Boulder Creek Area Madison County, Montana

Potential gold mines lie high among the rugged peaks of the Tobacco Root Mountains of southwestern Montana. This is a region where little geologic work has been done, though extensive mine operations have been carried on, and valuable ore has been shipped.