Análise microbiológica da exposição das roscas dos implantes osseointegráveis no seio maxilar de ovelhas

Pós-graduação em Odontologia - FOA

Contaminación microbiana en las líneas de agua de las unidades odontológicas

Introduction and Objective: Microorganisms are responsible for multiple infections and pathologies; this is why it is important to control microbes that can be found in the triple syringe used for irrigation in different dental procedures. The aim of this study was to identify cultivable species of microbes (fungus and bacteria) found in some dental units water lines of a private dental clinic from Medellín, Colombia. Materials and Methods: Random samples were taken from 11 chairs from a total of 89; a sample of water of 500ml was collected from the triple syringe of each selected chair. The study aim to search for the presence of total coliforms, filamentous fungi and leavened Results: the average presence of microorganisms was between 40CFU and more of 200 CFU. Microorganisms such as Aeromona salmonicida, Actinobacilus sp and Pseudomona maltophil were isolated. No total coliforms neither fecal coliforms were found. Conclusions: the high levels of contamination suggest that there is a mature biofilm in somewhere of the dental unit water line, but the absence of total and fecalis coliforms suggest that the water had been treated.

Genotoxic effect of photodynamic therapy mediated by curcumin on Candida albicans

Photodynamic therapy (PDT) is a promising method for localized and specific inactivation of fungi and bacteria. A nontoxic light-sensitive compound is taken up by cells, which are then exposed selectively to light, which activates toxicity of the compound. We investigated the potential of sublethal PDT using light-sensitive curcumin (CUR) in combination with blue (455 nm) light to promote reactive oxygen species (ROS) formation in the form of singlet oxygen and DNA damage of Candida albicans. Surprisingly, CUR-mediated PDT but also light alone caused significantly longer comet tails, an indication of DNA damage of C. albicans when compared with the negative control. The intracellular ROS production was also significantly higher for the group treated only with light. However, PDT compared to blue light alone significantly slowed DNA repair. Comet tails decreased during 30 min visualized as a 90% reduction in length in the absence of light for cells treated with light alone, while comet tails of cells treated with PDT only diminished in size about 45%. These results indicate that complex mechanisms may result in PDT in a way that should be considered when choosing the photosensitive compound and other aspects of the treatment design.

Caracterização dos mecanismos de ação da imunoterapia intravesical com P-MAPA envolvendo as vias de sinalização dor receptores Toll-like (TLR) 2 e 4 na progressão do câncer de bexiga não-músculo invasivo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação do potencial de degradação do herbicida diuron por fungos isolados de solo de canavial e suas tolerâncias aos metabólitos gerados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção de celulases fúngicas por fermentação em estado sólido: ampliação de escala de biorreatores de leito fixo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efetividade da higienação de brinquedos infantis quando comparada a nenhuma intervenção na redução de infecções microbianas em ambientes relacionados ao cuidado de crianças: revisão sistemática da literatura

Pós-graduação em Enfermagem - FMB

Planeamiento y análisis integral del paisaje de la cuenca hidrográfica del río buenavista provincia de Manabí-Ecuador para la implementación de políticas de incentivos a la restauración de ecosistemas con fines de conservación (programa socio Bosque-Ecuador)

Pós-graduação em Geografia - FCT

Recent advances in nanoparticle carriers for coordination complexes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The multifaceted roles of metabolic enzymes in the Paracoccidioides species complex

Paracoccidioides species are dimorphic fungi and are the etiologic agents of paracoccidioidomycosis, which is a serious disease that involves multiple organs. The many tissues colonized by this fungus suggest a variety of surface molecules involved in adhesion. A surprising finding is that most enzymes in the glycolytic pathway, tricarboxylic acid (TCA) cycle and glyoxylate cycle in Paracoccidioides spp. have adhesive properties that aid in interacting with the host extracellular matrix and thus act as 'moonlighting'proteins. Moonlighting proteins have multiple functions, which adds a dimension to cellular complexity and benefit cells in several ways. This phenomenon occurs in both eukaryotes and prokaryotes. For example, moonlighting proteins from the glycolytic pathway or TCA cycle can play a role in bacterial pathogenesis by either acting as proteins secreted in a conventional pathway and/or as cell surface components that facilitate adhesion or adherence. This review outlines the multifunctionality exhibited by many Paracoccidioides spp. enzymes, including aconitase, aldolase, glyceraldehyde-3-phosphate dehydrogenase, isocitratelyase, malatesynthase, triose phosphate isomerase, fumarase, and enolase. We discuss the roles that moonlighting activities play in the virulence characteristics of this fungus and several other human pathogens during their interactions with the host.

Evaluation of the presence of microorganisms in solid-organ preservation solution

OBJECTIVE: To assess the presence of microorganism contamination in the preservation solution for transplant organs (kidney/pancreas). Method: Between August 2007 and March 2008, 136 samples of preservation solution were studied prior to graft implantation. Variables related to the donor and to the presence of microorganisms in the preservation solution of organs were evaluated, after which the contamination was evaluated in relation to the recipient culture variable. Univariate and multivariate statistical analyses were performed. RESULTS: The contamination rate of the preservation solution was 27.9%. Coagulase-negative Staphylococcus was the most frequently isolated microorganism. However, highly virulent agents, such as fungi and enterobacteria, were also isolated. In univariate analysis, the variable donor antibiotic use was significantly associated to the contamination of the preservation solution. on the other hand, multivariate analysis found statistical significance in donor antibiotic use and donor's infectious complications variables. CONCLUSIONS: In this study, 27.9% of the preservation solutions of transplant organs were contaminated. Infectious diseases and non-use of antibiotics by the donor were significantly related to the presence of microorganisms in organ preservation solutions. Contamination in organ preservation solutions was not associated with infection in the recipient.

ITScan: a web-based analysis tool for Internal Transcribed Spacer (ITS) sequences

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Molar incisor hypomineralization: considerations about treatment in a controlled longitudinal case

Molar incisor hypomineralization (MIH) is a defect in the tooth enamel of systemic origin and may affect one or all four first permanent molars frequently associated with the permanent incisors. This case reports a 7-year-old child with severe MIH in the permanent molars associated with tooth decay and intense pain. In the first stage of treatment, therapy was performed with fluoride varnish and restoration with glass ionomer cement (GIC). After 6 years of clinical and radiographic follow-up, the restorations presented wear and fractures on the margins, indicating their replacement with composite resin. Severe cases of MIH in the early permanent molars can be treated with varnish and GIC to restore the patient's comfort and strengthen the hypomineralized dental structures. The clinical and radiographic monitoring frequently indicated when the restoration with composite resin should be performed.

Supressividade a Fusarium oxysporum f. sp. cubense por produtos orgânicos

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Assembleia, frugivoria e biologia reprodutiva de morcegos em áreas restauradas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)