ENaC activity in collecting ducts modulates NCC in cirrhotic mice.

Cirrhosis is a frequent and severe disease, complicated by renal sodium retention leading to ascites and oedema. A better understanding of the complex mechanisms responsible for renal sodium handling could improve clinical management of sodium retention. Our aim was to determine the importance of the amiloride-sensitive epithelial sodium channel (ENaC) in collecting ducts in compensate and decompensate cirrhosis. Bile duct ligation was performed in control mice (CTL) and collecting duct-specific αENaC knockout (KO) mice, and ascites development, aldosterone plasma concentration, urinary sodium/potassium ratio and sodium transporter expression were compared. Disruption of ENaC in collecting ducts (CDs) did not alter ascites development, urinary sodium/potassium ratio, plasma aldosterone concentrations or Na,K-ATPase abundance in CCDs. Total αENaC abundance in whole kidney increased in cirrhotic mice of both genotypes and cleaved forms of α and γ ENaC increased only in ascitic mice of both genotypes. The sodium chloride cotransporter (NCC) abundance was lower in non-ascitic KO, compared to non-ascitic CTL, and increased when ascites appeared. In ascitic mice, the lack of αENaC in CDs induced an upregulation of total ENaC and NCC and correlated with the cleavage of ENaC subunits. This revealed compensatory mechanisms which could also take place when treating the patients with diuretics. These compensatory mechanisms should be considered for future development of therapeutic strategies.

La flûte enchantée. Ah! l'ingrat par son silence : air de Pamina / Mozart, comp.. Roméo et Juliette. Ah! je veux vivre dans le rêve : valse / Gounod, comp. ; Mme Ritter Ciampi, S ; accompagnement d'orchestre

Comprend : La flûte enchantée ; Roméo et Juliette

Airs de Ballet : Marche, Air gai en rondeau, Tambourins... / Jean-Philippe Rameau, comp. ; Mme Pauline Aubert, clav. ; orchestre sous la direction de M. R. Gerlin

Comprend : Marche / Jean-Philippe Rameau, comp. ; Mme Pauline Aubert, clav. ; orchestre sous la direction de M. R. Gerlin ; Air gai en rondeau / Jean-Philippe Rameau, comp. ; Mme Pauline Aubert, clav. ; orchestre sous la direction de M. R. Gerlin ; Tambourins / Jean-Philippe Rameau, comp. ; Mme Pauline Aubert, clav. ; orchestre sous la direction de M. R. Gerlin ; Menuets / Jean-Philippe Rameau, comp. ; Mme Pauline Aubert, clav. ; orchestre sous la direction de M. R. Gerlin ; Rondeau / Jean-Philippe Rameau, comp. ; Mme Pauline Aubert, clav. ; orchestre sous la direction de M. R. Gerlin ; Air / Jean-Philippe Rameau, comp. ; Mme Pauline Aubert, clav. ; orchestre sous la direction de M. R. Gerlin

L'Amour garde du corps . Air : Ce mouchoir, belle Raimonde, &c.

Comprend : Chanson bachique d'un soldat, sur l'air d'une contre-danse allemande ; Romance, sur l'air : "Charmante Gabrielle, &c." ; V'la c'te chanson véritable par laquelle on prouve, comme quoi après la pluie vient l'beau tems