喀斯特植物适应高钙的方式与干旱及外源钙诱导基因的克隆和表达

喀斯特环境是一类发育在可溶性碳酸盐类岩石上的环境系境，其生境具有高钙和干早等特征。喀斯特生境中的优势/特征植物也多具适应高钙、耐旱的特性。旋蒴苣苔(Boea hygrometrica)是喀斯特地区一种典型的抗旱和适应高钙的植物。本文在探讨了喀斯特地区植物适应高钙环境的不同方式的基础上，从旋蒴苣苔中克隆了两个受脱水和外源Ca2+诱导表达的基因，并对其表达调控和蛋白质产物进行了分析。本研究将为治理西南喀斯特地区日益退化的生态系统提供理论基础。 本文测定了贵州4个地区采集地内45种优势/常见种的地上部分和地下部分的全钙含量以及土壤的交换性钙含量。通过分析喀斯特地区植物与土壤钙含量的特征发现：喀斯特地区植物具有较高的钙含量平均值；土壤交换性钙含量对植物地上部钙含量的影响总体上不显著，对植物地下部钙含量的影响显著；不同类别的植物钙含量存在显著差异，蕨类植物地上部钙含量平均值明显低于被子植物；不同类别植物的钙的分布部位也存在显著差异，在蕨类植物和单子叶植物中地上部和地下部的钙含量相近，而双子叶植物的地上部钙含量明显高于地下部。根据地上部分与地下部分钙含量的差异性以及与土壤交换性钙含量的相关关系将其中14种优势植物对土壤高钙的适应方式分为3种类型：随遇型，高钙型和低钙型。随遇型植物地上部和地下部的钙含量均与土壤交换性钙含量成显著正相关关系；高钙型植物具有较强的钙富集能力，其地上部即使在低钙含量的土壤中也可维持较高的钙含量；低钙型植物的地上部即使在高钙的土壤中亦可维持较低的钙含量。对贵州和北京等地采集到的旋蒴苣苔钙含量和土壤含钙量的分析发现，其地上部和地下部钙含量即使在高钙土中也呈低水平，推测可能是通过控制根部对钙的吸收和向上运输来维持低钙水平。 从旋蒴苣苔cDNA 文库中筛选到两个片段，经5’RACE 得到cDNA 全长，分别命名为BhOMT1 和BhC2DP1。BhOMT1 编码一个包含359 个氨基酸的蛋白质，蛋白序列分析表明BhOMT1 为植物O-甲基转移酶。BhOMT1 基因组序列包含一个内含子，与其同源基因的基因结构相似。BhC2DP1 编码一个包含154 氨基酸的小分子蛋白质，内含一个C2 结构域，该结构域在很多植物蛋白中均存在。通过5’RAGE 的方法分别克隆了1465bp BhOMT1 启动子序列和1113bp 的BhC2DP1 启动子序列，分析发现其中均含有多种逆境胁迫诱导元件。通过对BhOMT1 和BhC2DP1 表达分析和Western 杂交发现这两个基因均受脱水和外源Ca2+的诱导表达，表明这两个基因可能参与了旋蒴苣苔的脱水保护和对高钙环境的适应。

Impact of mechanisation of fisheries development in Karnataka

Karnataka, one of the maritime states on the west coast of India, has progressed quite well in marine fisheries due to its vast fisheries resources and diversification in mechanised fishing. Mechanisation programmes were started by the state from 1957-58 only. Starting with two small mechanised boats, the state has today a fishing fleet of 398 purseseiners, 731 gillnetters, 2 deepsea trawlers and about 1,500 shrimp trawlers contributing over 85% of the total marine fish landings. The marine fish production during 1987-88 up to the end of March 1988 was 1,29,659 tonnes valued at Rs.48.05 crores.

Effect of different media on the determination of the number of micro organisms in frozen prawns (shrimp)

The Indian standards Institution have recently laid down specifications for the grading of frozen shrimp of which the bacterial examination of the product forms a significant part. Work carried out in this regard in our laboratories have shown that various improvements could be made in the composition of the medium to obtain a more true picture of the total bacterial count. Changes in the medium for total bacterial count have, therefore, been suggested and the reasons for the same discussed.

Studies on the gonadosomatic index and fecundity of chapila (Gudusia chapra Ham.)

The study was conducted in pond to determine the fecundity and gonadosomatic index of chapila, Gudusia chapra. The male fish was found to attain sexual maturity at 7.7 cm and 7.41 g and that of the female at 9.3 cm and 14.65 g by standard-length and body-weight respectively. The investigated fishes were found to be male and female at the ratio of 1:3 and generally female was found to be larger than male. The fish was found to spawn for several months with two spawning peaks, one in April and another in August as indicated by the peaks of gonadosomatic index and ova diameter. Fecundity of the fish ranged from 25,220 to 154,528 with an average value of 72,383 and was found to increase with the increase in length and weight of the fish. The relationships between fecundity and standard-length, body-weight, gonad-length and gonad weight of the fish were found to be linear and significant.

莱州湾区域表层沉积物中多氯化萘、多溴联苯醚和氯化石蜡的研究

本研究建立了沉积物中氯化石蜡（CPs）的分析方法，对莱州湾和该区域主要河流的表层沉积物中多氯萘（PCNs），多溴联苯醚（PBDEs）和CPs进行了分析，获得了他们在研究区域的含量水平、空间分布和单体分布模式，并初步探讨了它们在环境中的来源和迁移规律，估算了这三类污染物在莱州湾表层沉积物中的储量，初步评估了莱州湾沿岸的工业排放与人类活动对海洋生态环境的影响。 莱州湾海洋和河流沉积物PCNs的含量分别为65-470 pg/g dw，52-5100 pg/g dw，平均值分别为260 pg/g dw，1100 pg/g dw。莱州湾沉积物中PCNs的含量较低，与发达国家的背景值相当，主要河流沉积物与其他地区的近海、海湾、湖泊沉积物的含量相当。该区域的PCNs主要以5-Cl和6-Cl为主，为工业来源，主要受化工行业的影响。石化工厂热过程产生的低氯组分可能也是该区域PCNs的一个重要来源。莱州湾的PCNs主要来自水体颗粒的输入，而大气沉降来源的贡献不明显。 河流沉积物ΣPBDEs（不包括BDE-209）的含量分别为0.01- 53 ng/g dw，BDE-209的含量为0.74- 280 ng/g dw，平均值分别为4.4 ng/g dw，51 ng/g dw。在组成上，BDE-209占绝对优势，比低溴代BDEs高1-2个数量级，这是由于我国市场上十溴联苯醚是最主要的溴代阻燃剂。ΣPBDEs与亚洲一些地区含量相当，比北美和欧洲一些地区要低；BDE-209含量含量高出北美和欧洲一些地区，处于较高的水平。河流沉积物中BDE-47与-99，BDE-183与-153、-154具有很好的相关性，而且这几种单体质量分数相当，说明莱州湾区域存在五溴和八溴两种工业BDE阻燃剂来源。海洋沉积物中ΣPBDEs的含量为nd- 0.66 ng/g dw，平均值为0.32 ng/g dw，处于较低水平，BDE-209的含量为0.66- 12 ng/g dw，平均值为5.1 ng/g dw，与欧美一些地区相当，主要来自水体颗粒的输入。 本研究用电子捕获低分辨质谱（ECNI-LRMS）建立了沉积物中CPs的分析方法。该方法基于ECNI质谱对SCCP的响应与其氯含量在一定范围内呈线性关系的特性，建立SCCP的总响应因子与氯含量的工作曲线，从而建立定量方法。重点改进了传统方法中由于SCCP标准品和样品中氯含量不同所造成的响应因子的差别，不再要求标准品的含氯量与样品一致，从而提高了SCCP的分析效率与方法准确性。实验发现，当SCCP的实际氯含量在51%-63%之间时，二者线性关系良好（r2>0.96）。用不同氯含量的标准参考品测试，误差为8%-43%。仪器检测限和方法检测限分别为25-400 μg/L，20 ng/g。类似地，建立了中链氯化石蜡（MCCP）的分析方法，工作曲线的实际氯含量范围为44-57%，仪器检测限40-600 μg/L，方法检测限为6 ng/g。通过对比样品与标准品的峰形特征，严格控制保留时间来辨别SCCP与MCCP，实验证明由于二者叠加导致的误差：SCCP为8%，MCCP为14%，总体来说，用该方法得到的CPs数据是可靠的。本文所使用的净化方法能实现CPs与大部分有机氯化合物的分离。 用该方法对莱州湾的沉积物的CPs进行定量，除了两个河流样品MCCP的计算含氯量低于工作曲线以外，其他样品的SCCP和MCCP的计算氯含量都在工作曲线的范围之内。河流沉积物中SCCP和MCCP的含量分别为1-1200 ng/g dw，1-3300 ng/g dw，MCCP/SCCP均值为1.2；含量分布变化较大，一些河段受到工业排放的影响，CPs浓度高出均值1-2个数量级，MCCP/SCCP值也显著高出平均值。莱州湾区域大部分河流的CPs含量与欧洲、北美和日本一些地区相当，而一些受到点源污染的河段则处于较高的水平。河流中SCCP的质量分布在不同的站位不一样，在受到点源影响的样品中，C13的质量分数明显增大，占优势地位，可能是由MCCP携带的SCCP组分所致。MCCP的质量分布在河流和海洋中没有区别，都是C14占绝对优势。从氯取代的组分来讲，所有样品都是以6-8氯取代为主。海洋沉积物中SCCP和MCCP的含量分别为3-18 ng/g dw，1-13 ng/g dw， MCCP/SCCP均值为0.68，显著低于河流沉积物；SCCP的质量分布较为一致，都是以C10和C11为主。研究发现莱州湾区域SCCP与MCCP来源相似，但迁移规律有的不同。海洋环境中的MCCP可能主要来自水体颗粒的输入，而溶解态输入也是海洋环境中SCCP的重要来源。 对这三类污染物在莱州湾表层沉积物中的储量估算得到PCNs、ΣPBDEs、BDE-209、SCCP和MCCP在莱州湾表层0-10 cm沉积物中的储量分别为0.20t，0.25t，3.95t，6.52t，4.30t。拥有10%的渤海海域面积的莱州湾ΣPBDEs和BDE-209的储量分别占渤海海区总量的18-23%，18-32%，远远超过渤海的平均值。

Widening engineering horizons: Addressing the complexity of sustainable development

The complex, fragmented and diverse aspects of a sustainable development perspective are translated into an eight-point framework that defines a problem boundary larger than that traditionally adopted by civil engineers. This leads to practical questions intended to inform engineers who ask 'am I being sustainable?' during project implementation. The value of the questions is tested against a case history of a wastewater treatment project. This demonstrates the relevance of the questions to successive project delivery phases of defining the problem, choosing a solution and implementing that solution through design, construction and operation. The case history highlights that answers to several of the additional questions raised by considering this wider problem space are currently buried within government and clients' policies, regulations and standard practice; these answers may not be accessible to the professional engineer.

Rapid evolution, genetic variations, and functional association of the human spermatogenesis-related gene NYD-SP12

NYD-SP12 is a recently identified spermatogenesis-related gene with a pivotal role in human testis development. In this study, we analyzed between-species divergence and within-species variation of NYD-SP12 in seven representative primate species, four wo

Numerical simulation of the growth of ZnO nanostructures in a tube furnace by physical vapour deposition

Zinc oxide is a versatile II-VI naturally n-type semiconductor that exhibits piezoelectric properties. By controlling the growth kinetics during a simple carbothermal reduction process a wide range of 1D nanostructures such as nanowires, nanobelts, and nanotetrapods have been synthesized. The driving force: for the nanostructure growth is the Zn vapour supersaturation and supply rate which, if known, can be used to predict and explain the type of crystal structure that results. A model which attempts to determine the Zn vapour concentration as a function of position in the growth furnace is described. A numerical simulation package, COMSOL, was used to simultaneously model the effects of fluid flow, diffusion and heat transfer in a tube furnace made specifically for ZnO nanostructure growth. Parameters such as the temperature, pressure, and flow rate are used as inputs to the model to show the effect that each one has on the Zn concentration profile. An experimental parametric study of ZnO nanostructure growth was also conducted and compared to the model predictions for the Zn concentration in the tube. © 2008 Materials Research Society.

Effect of feeding frequency on the growth of common carp (Cyprinus carpio L.) fry

A laboratory-feeding trail was conducted for 45 days with fry of common carp Cyprinus carpio L. (0.45±0.03g) in aquaria in a static indoor fish rearing system. The fry were fed on a pelleted diet containing 33% crude protein having fishmeal as major protein source. The fish fry in five treatments A, B, C, D, and E, each with two replicates were fed on 5% daily ration divided into different feeding frequencies of 2, 3, 4, 5 and 6 times a day respectively in order to observe the growth performance. Each replicate contained 15 fry having total initial weight of 6.87±0.31g. At the end of the feeding trial, significantly different and higher (p<0.05) growth response was observed in treatment C having a feeding frequencies of 4 times a day. Significantly the highest and the lowest percent growth of 334.30 and 218.91% were observed in fish fed on the diet (Treatment C) with 4 times and (Treatment A) 2 times feeding frequencies per day, respectively. Food conversion ratio (FCR) of 1.78 was significantly higher (p

An illustrated key to the Malacostraca (Crustacea) of the northern Arabian Sea. Part 3: Euphausiacea

The key includes twenty-one species of euphausiids belonging to two families and six genera. The key was prepared following Brinton (1975). Since several authors attributed a fundamental importance to thelycum in systematics of euphausiids therefore the available figures of thelycum are also included.