Water quality and nutrient dynamics - Lake Victoria, Kenya

Limnological studies in Lake Victoria (Kenyan portion) have been sporadic. Water quality and nutrient dynamics studies are being undertaken in fifteen sampling sites that have been divided into four ecological zones namely: Nyanza Gulf, Rusinga Channel, open waters inshore and open waters. The ongoing study will show how the physical and chemical paramenters affect fish distribution and abundance.

K418合金和42CrMo钢激光焊焊缝的微观组织

采用透射电镜及附带能谱(TEM-EDS)对K418高温合金涡轮盘和42CrMo合金钢转轴激光焊焊缝的微观组织进行了分析.结果表明:焊缝主要由树枝状非平衡凝固的FeCrNiC(γ)固溶体组成,此外,还有少量细小、弥散的Ni_3Alγ'相、Laves颗粒和少量MC碳化物分布在树枝晶之间区域;根据热动力学原理对形成这一组织特征的原因进行了简要讨论.

The diagnostics of density distribution for inhomogeneous dense DT plasmas using fast protons

The density distribution of inhomogeneous dense deuterium-tritium plasmas in laser fusion is revealed by the energy loss of fast protons going through the plasma. In our simulation of a plasma density diagnostics, the fast protons used for the diagnostics may be generated in the laser-plasma interaction. Dividing a two-dimensional area into grids and knowing the initial and final energies of the protons, we can obtain a large linear and ill-posed equation set. for the densities of all grids, which is solved with the Tikhonov regularization method. We find that the accuracy of the set plan with four proton sources is better than those of the set plans with less than four proton sources. Also we have done the density reconstruction especially. for four proton sources with and without assuming circularly symmetrical density distribution, and find that the accuracy is better for the reconstruction assuming circular symmetry. The error is about 9% when no noise is added to the final energy for the reconstruction of four proton sources assuming circular symmetry. The accuracies for different random noises to final proton energies with four proton sources are also calculated.

Prevalence and magnitude of body weight and image dissatisfaction among women in dietetics majors

[EN] Some authors have suggested that body weight dissatisfaction may be high in students majoring in dietetics. Therefore, this study was conducted to examine the extent of body weight and image dissatisfaction in a sample of women in dietetics major. Additionally, predictors of magnitude of body weight dissatisfaction were analyzed. Participants were 62 volunteers with normalweight whose mean age was 21.87±1.89 years old (nonrandom sample). The assessment instruments included anthropometric measurements, a somatomorphic matrix test and an eating disorders inventory (EDI-2). Data were analyzed using SPSS vs. 15.0. A larger proportion of students chose an ideal body weight lower than actual weight (67.7%) and body image with less body fat and more muscle mass than actual values (56.4%). The magnitude of body weight dissatisfaction was associated with muscle mass and body fat dissatisfaction, and with the subscale of EDI-2 “body dissatisfaction”. So, from a public health standpoint, we consider important to continue working in this line of research with the aim of better understanding the extent of body weight dissatisfaction in women dietitians, and how this dissatisfaction could interfere with their professional practice.

Sobrepeso, obesidad y bajo peso en niños tratados por el Servicio de Urgencias de Pediatría.

[ES] En el presente trabajo se estudiaron de forma transversal 235 niños con edades comprendidas entre los 2 y 14,9 años. El 6% de la muestra total presentó bajo peso, y el 17% sobrepeso u obesidad. Por lo que concluimos que los servicios de pediatría deberían tener un papel activo en el control y prevención de las alteraciones del estado nutricional.

Revealing, illuminating, and modifying proteins in human diseases using noncanonical amino acids

To better understand human diseases, much recent work has focused on proteins to either identify disease targets through proteomics or produce therapeutics via protein engineering. Noncanonical amino acids (ncAAs) are tools for altering the chemical and physical properties of proteins, providing a facile strategy not only to label proteins but also to engineer proteins with novel properties. My thesis research has focused on the development and applications of noncanonical amino acids in identifying, imaging, and engineering proteins for studying human diseases. Chapter 1 introduces the concept of ncAAs and reveals insights to how I chose my thesis projects.

ncAAs have been incorporated to tag and enrich newly synthesized proteins for mass spectrometry through a method termed BONCAT, or bioorthogonal noncanonical amino acid tagging. Chapter 2 describes the investigation of the proteomic response of human breast cancer cells to induced expression of tumor suppressor microRNA miR-126 by combining BONCAT with another proteomic method, SILAC or stable isotope labeling by amino acids in cell culture. This proteomic analysis led to the discovery of a direct target of miR-126, shedding new light on its role in suppressing cancer metastasis.

In addition to mass spectrometry, ncAAs can also be utilized to fluorescently label proteins. Chapter 3 details the synthesis of a set of cell-permeant cyclooctyne probes and demonstration of selective labeling of newly synthesized proteins in live mammalian cells using azidohomoalanine. Similar to live cell imaging, the ability to selectively label a particular cell type within a mixed cell population is important to interrogating many biological systems, such as tumor microenvironments. By taking advantage of the metabolic differences between cancer and normal cells, Chapter 5 discusses efforts to develop selective labeling of cancer cells using a glutamine analogue.

Furthermore, Chapter 4 describes the first demonstration of global replacement at polar amino acid positions and its application in developing an alternative PEGylation strategy for therapeutic proteins. Polar amino acids typically occupy solvent-exposed positions on the protein surface, and incorporation of noncanonical amino acids at these positions should allow easier modification and cause less perturbation compared to replacements at the interior positions of proteins.

Die Herings-Polarstamm-Theorie 250 Jahre alt - und aktuelle Bezüge

250 years ago, in Hamburg a herring polar migration theory was published as part of the "Nachrichten von Island, Grönland und der Straße Davis" from the literary remains of the mayor Dr. Johann Anderson. Based on various sources and capable to answer to the herring questions of the time, this one-stock-migration theory became international hypothesis during the following tenth of years. It still is classified as remarkable because some actual ideas on herring migration were thought already 250 years ago. Abstract DE: In Hamburg wurde vor 250 Jahren eine Polarstamm-Theorie zur Heringswanderung als Teil der Nachrichten von Island, Grönland und der Strasse Davis aus dem Nachlass des Bürgermeisters Dr. Johann Anderson veröffentlicht. Aufbauend auf unterschiedliche Quellen und in der Lage, die damaligen Fragen um den Hering zu beantworten, wurde diese Ein-Stamm-Theorie zur heringswanderung über Jahrzehnte internationale Lehrmeinung. Sie wird auch heute noch als bemerkenswert eingestuft. Denn sie enthielt vor 250 Jahren schon Gedankengänge, die noch aktuell sind.

Surface-enhanced resonance Raman scattering spectroscopy of single R6G molecules

Surface-enhanced resonance Raman scattering (SERRS) of Rhodamine 6G (R6G) adsorbed on colloidal silver clusters has been studied. Based on the great enhancement of the Raman signal and the quench of the fluorescence, the SERRS spectra of R6G were recorded for the samples of dye colloidal solution with different concentrations. Spectral inhomogeneity behaviours from single molecules in the dried sample films were observed with complementary evidences, such as spectral polarization, spectral diffusion, intensity fluctuation of vibrational lines and even "breathing" of the molecules. Sequential spectra observed from a liquid sample with an average of 0.3 dye molecules in the probed volume exhibited the expected Poisson distribution for actually measuring 0, 1 or 2 molecules. Difference between the SERRS spectra of R6G excited by linearly and circularly polarized light were experimentally measured.

1976 step in the Pacific climate: forty environmental changes between 1968-1975 and 1977-1984

Examination of 40 time series of multidisciplinary environmental variables from the Pacific Ocean and the Americas, collected in 1968 to 1984, demonstrated the remarkable consistency of a major climate-related, step-like change in 1976. To combine the 40 variables (e.g., air and water temperatures, Southern Oscillation, chlorophyll, geese, salmon, crabs, glaciers, atmospheric dust, coral, carbon dioxide, winds, ice cover, Bering Strait transport) into a single time series, standard variants of individual annual values (subtracting the mean and dividing by a standard deviation) were averaged. Analysis of the resulting time series showed that the single step in 1976, separating the 1968-1975 period from the 1977-1984 period, accounted for 89% of variance within the composite time series. Apparently, one of the Earth's large ecosystems occasionally undergoes large abrupt shifts.

Factors influencing the downstream transport of sediment in the Lough Feeagh catchment, Burrishoole, Co. Mayo, Ireland

Research laboratories in the Burrishoole catchment have been the focus of salmonid research since 1955. One aspect of the research has been to monitor the number of salmon and sea trout migrating to sea as smolts and returning to the catchment as adults. In the early 1990s it became clear that the smolt output from the catchment had declined over the previous two decades. At about the same time the presence of fine particles of peat silt in the hatchery became increasingly apparent and led to a higher incidence of mortality of young fry. These observations and management difficulties led to a study of silt transport in the surface waters of the catchment, which is described in this article. The authors describe geology, soils, climate and hydrology of Burrishoole before examining the sediment deposition in Lough Feeagh.