992 resultados para nutrient contents


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Intrauterine dietary restriction may cause changes in the functioning of offspring organs and systems later in life, an effect known as fetal programming. The present study evaluated mRNA abundance and immunolocalization of nutrient transporters as well as enterocytes proliferation in the proximal, median and distal segments of small intestine of rats born to protein-restricted dams. Pregnant rats were fed hypoproteic (6% protein) or control (17% protein) diets, and offspring rats were evaluated at 3 and 16 weeks of age. The presence of SGLT1 (sodium-glucose co-transporter 1), GLUT2 (glucose transporter 2), PEPT1 (peptide transporter 1) and the intestinal proliferation were evaluated by immunohistochemical techniques and the abundance of specific mRNA for SGLT1, GLUT2 and PEPT1 was assessed by the real-time PCR technique. Rats born to protein-restricted dams showed higher cell proliferation in all intestinal segments and higher gene expression of SGLT1 and PEPT1 in the duodenum. Moreover, in adult animals born to protein-restricted dams the immunoreactivity of SGLT1, GLUT2 and PEPT1in the duodenum was more intense than in control rats. Taken together, the results indicate that changes in the small intestine observed in adulthood can be programmed during the gestation. In addition, they show that this response is caused by both up-regulation in transporter gene expression, a specific adaptation mechanism, and intestinal proliferation, an unspecific adaptation mechanism.

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The way of applying zinc can influence the zinc uptake and productivity of crops, especially cereals that have high demand for this nutrient. The aim of this study is to evaluate methods of Zn application on soil, nutritional status and productivity of maize. For this, an experiment was undertaken at FCAV/UNESP, Jaboticabal-SP, in Oxisol clay (DTPA on Zn: 0.5 mg dm-3) with maize (hybrid Simple Impact), from December through May 2009. Nine treatments with three doses of Zn in soil banded application (in furrows) and three doses of Zn by incorporation in soil (0-20 cm depth), foliar application, seed application and control (no Zn). The treatments were arranged in a randomized block design with four replications. Regardless of the method, Zn application promoted higher contents of this micronutrient in soil and higher accumulation in the shoots as well as increasing Zn in the maize grain. However, it did not affect the nutritional status and yield of the maize. The Zn application in the soil resulted in a greater Zn uptake by plants and maize yield, compared to Zn application in the plant by seed or foliar.

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The recalcitrance in grasses varies according to cell type and maturation. The origin of the recalcitrance in different regions from sugar cane internodes with varied lignin contents was evaluated. The efficiency of enzymatic hydrolysis was correlated with the chemical, micromorphological and microspectrophotometric characteristics of the samples. The internodes of three sugar cane hybrids were dissected into four different fractions. The outermost fraction and the rind were the most recalcitrant regions, whereas the pith-rind interface and the pith were less recalcitrant. Cellulose conversion reached 86% after 72h of enzymatic digestion of the pith from the hybrid with the lowest lignin content. There was an inversely proportional correlation between the area occupied by vascular bundles and the efficiency of cellulose hydrolysis. High cellulose and low lignin or hemicellulose contents enhanced the efficiency of enzymatic hydrolysis of the polysaccharides. The critical evaluation of the results permitted to propose an empirical parameter for predicting cellulose conversion levels that accounts for the positive effect of high cellulose and low lignin plus hemicellulose and the detrimental effect of abundant vascular bundles. The cellulose conversion levels fit well to this calculated parameter, following a second order polynomial with an r2 value of 0.96. © 2013 Elsevier B.V.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Pós-graduação em Agronomia (Ciência do Solo) - FCAV

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Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE

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Pós-graduação em Agronomia (Energia na Agricultura) - FCA

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Agronomia (Horticultura) - FCA

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Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV