968 resultados para laboratory experiment


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Penaeus merguensis is so far reported to attain complete maturity and spawn in the sea or deep culture ponds only. Mature specimens of stage III to V collected from a shallow reservoir of solar saltworks were studied and spawned in laboratory. A comparison of spawning of spawner from sea and reservoir is also reported.

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Milkfish fry were artificially bred and reared in the laboratory and the pigmentation pattern of the different developmental stages of the larvae are described in detail, with illustrations.

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A brief account is given of experiments undertaken rearing Penaeus monodon larvae fed on diatom (Chaetoceros calcitran) and fermented vegetable trash, which included fruits and their peels, vegetables and rice. The possible use of high protein content trash materials as a feed substitute is examined briefly.

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A simple statistical index, for evaluating the condition of growth in an aquaculture experiment and indicating the extent of effect of any plausible rival hypothesis, is presented.

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Synchronization phenomena in a fluid dynamical analogue of atmospheric circulation is studied experimentally by investigating the dynamics of a pair of thermally coupled, rotating baroclinic annulus systems. The coupling between the systems is in the well-known master-slave configuration in both periodic and chaotic regimes. Synchronization tools such as phase dynamics analysis are used to study the dynamics of the coupled system and demonstrate phase synchronization and imperfect phase synchronization, depending upon the coupling strength and parameter mismatch.

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Mud crablings, Scylla sp. were reared, for a period of six weeks in fiberglass aquarium under laboratory conditions, to determine the effect of four different stocking densities of 1, 2, 3 and 4 crabling/l of water on their survival and growth. Salinity of water was maintained at 25 ppt throughout the rearing period. Stocking rates of 1, 2, and 3 crabling/l resulted in a similar (p>0.05) survival rates of 75, 74, and 83.5%, respectively, that of 4 crabling/l resulted in significantly lower (p<0.05) survival rate of 56%. No significant difference was observed among different stocking densities in average growth of carapace length (CL), carapace width (CW) and body weight (BW).

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An experiment was carried out in the fields of the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh, Bangladesh to determine the impact of common carp (Cyprinus carpio) and tilapia (Oreochromis niloticus) culture on arthropod population, zoobenthos and weeds of rice field. The treatments were: (1) rice combined with mono sex tilapia, (2) rice combined with common carp, (3) rice combined with a mixed culture of mono sex tilapia and common carp and (4) rice alone. It was observed that tilapia significantly reduced the number of arthropods, green leafhoppers and white leafhoppers in the rice-fish production systems. Benthos analysis revealed significant effects of fish culture on the abundance of molluscs, oligochaete worms and chironomid larvae. Lowest number of benthos was obtained in the treatments with common carp and significant reduction of the weed biomass was observed, especially in the tilapia containing plots. Based on the results of the study it can be suggested that common carp may play an important role in controlling of benthic organisms, while tilapia might be more effective to control terrestrial arthropods and weeds.

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A laboratory based 2x3 factorial experiment was conducted for 12 weeks to investigate the influences of dietary lipid and phosphorus (P) levels on retention and excretion of phosphorus and nitrogen (N) in fingerling red sea bream. Two levels of lipid (210 and 260 g/kg) and three levels of phosphorus (17, 14 and 12 g/kgˉ¹) in the dry diets were tested. Duplicate groups of 25 red sea bream (average weight 3.74±0.07 g) per 60L glass tank were fed experimental diets three times a day near to satiation level at 22 to 28°C water temperature. A reduction in dietary fish meal from 500 to 300 g/kg dry diet, corresponding to a supplementation in both dietary lipid and P resulted in significant increase in both P and N retention which resulted in the reduction of their excretion by red sea bream. The overall results of the present study demonstrated that both lipid and phosphorus supplementation are necessary for developing less-polluting feed which in turn, reduce fish meal level in the diet of fingerling red sea bream. Further studies in this regard with different size and age groups of red sea bream are warranted.

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Spirulina is a filamentous cyanobacteria with many applications in food and drug industries, as a food in human, aquaculture, vet and poultry industries… . Semi and mass culture of Spirulina carries out in different countries. This study was carried out in five phases in order to produce this microalga in Iran. The first phase, Spirulina pure stock was imported from Indonesia. After identification of species, it was cultured in laboratory until we took 20 liters of biomass. The semi-mass culture was carried out in green house. Cell concentration and size of Spirulina were recorded during culture daily and their growth rates were calculated. After two weeks, when the size of Spirulina was suitable, biomass of Spirulina was harvested then accumulated Spirulina weighted and dried in 24 hours in laboratory. In order to microbiological study, the samples of Spirulina (dry and fresh) were cultured on blood agar medium and coliforms were counted. The chemical composition of produced Spirulina was measured by standard methods. Fatty acid and amino acid profiles were acquired by GC and HPLC instruments, respectively. The amount of chlorophyll in Spirulina was determined by spectroscopy method. Also astaxanthin pigment as an important carotenoid was measured by HPLC in Spirulina and Penaeus semisulcatus larvae fed on Spirulina. At final phase of this project, larva fed on produced Spirulina (biomass and dry powder) was compared to Z plus, microencapsulated Spirulina (M.C.F) and Chaetoceros algae as control. This experiment was carried from zoa to early post larvae stage then survival and growth rate of larvae were recorded. The growth rate of larvae was evaluated with ANOVA test and survival rate of treatments was assessed by Log Rank (Mantel –Cox) test. Also during larvae stage, two parameters of water such as nitrate and nitrite were measured in zoa, mysis and post larvae stages. The results of this study were shown that colifom counts were 1.85×106 and 92.3×105 coliform per ml in fresh and dry spirulina, respectively. Protein percent of dry spirulina was 50.93 % (dry weight) and the amount of astaxanthin in spirulina and larvae fed on spirulina were 0.21 and 0.01 mg/kg, respectively. The most survival rate of larvae were observed in zoa III (88.8%) with Z plus supplement treatment, in mysis III (76.5%) combination of Z plus and dry spirulina in comparative between treatments. Larvae growth (4.5mm) of control in early post larvae was the best.

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Established firms accumulate a significant body of knowledge, expertise and capabilities that are often secondary to their central revenue generating activities. How do they leverage this expertise in non-core technology into future value creation opportunities? In this paper we examine an attempt by the telecommunications firm BT to create value from the accumulated knowledge within its laboratories by setting up an incubator. While conceived by the board as a mechanism for leveraging the value of non-core technology into the workplace, corporate support for the incubator was withdrawn after only three years and prompted the incubator to partner with a venture capital firm, NVP, in the spin-out of ventures. Through analysis of this single case we observe how entering into such a relationship reduces the transaction costs of accessing complementary resources, capabilities and competences, while simultaneously reducing a number of the risks associated with venturing for both parties. Partnering with the venture capitalist allows the established firm to get its intellectual property into the market, for it to be tested by the market and further developed. © 2010 Inderscience Enterprises Ltd.

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Kolmogorov's two-thirds, ((Δv) 2) ∼ e 2/ 3r 2/ 3, and five-thirds, E ∼ e 2/ 3k -5/ 3, laws are formally equivalent in the limit of vanishing viscosity, v → 0. However, for most Reynolds numbers encountered in laboratory scale experiments, or numerical simulations, it is invariably easier to observe the five-thirds law. By creating artificial fields of isotropic turbulence composed of a random sea of Gaussian eddies whose size and energy distribution can be controlled, we show why this is the case. The energy of eddies of scale, s, is shown to vary as s 2/ 3, in accordance with Kolmogorov's 1941 law, and we vary the range of scales, γ = s max/s min, in any one realisation from γ = 25 to γ = 800. This is equivalent to varying the Reynolds number in an experiment from R λ = 60 to R λ = 600. While there is some evidence of a five-thirds law for g > 50 (R λ > 100), the two-thirds law only starts to become apparent when g approaches 200 (R λ ∼ 240). The reason for this discrepancy is that the second-order structure function is a poor filter, mixing information about energy and enstrophy, and from scales larger and smaller than r. In particular, in the inertial range, ((Δv) 2) takes the form of a mixed power-law, a 1+a 2r 2+a 3r 2/ 3, where a 2r 2 tracks the variation in enstrophy and a 3r 2/ 3 the variation in energy. These findings are shown to be consistent with experimental data where the polution of the r 2/ 3 law by the enstrophy contribution, a 2r 2, is clearly evident. We show that higherorder structure functions (of even order) suffer from a similar deficiency.