936 resultados para feces collection
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Pós-graduação em Biociências - FCLAS
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Objective In studies on Candida albicans adhesion to surfaces, diverse protocols have been used for collection and preparation of saliva samples. Thus, this study investigated whether variations in the centrifugation parameters and number of donors of saliva would influence the adhesion of C. albicans to a denture base resin. Methods Resin acrylic samples (n = 72) were made and then divided into four groups: (a) control – specimens were left without preconditioning in saliva; (b) three experimental groups, in which the specimens were preconditioned with saliva collected from 15 volunteers and centrifuged at 12 000 g for 5 min (G1); from 15 volunteers and centrifuged at 18 000 g for 30 min (G2); and from one volunteer and centrifuged at 12 000 g for 5 min (G3). Candida adhesion was evaluated by both the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) reduction method and crystal violet staining. Data were analyzed by one-way analyses of variance (P = 0.05). Results For XTT reduction assay, groups G2, G3, and control were not significantly different, whereas group G1 showed significantly higher absorbance value than control. For crystal violet staining there were no significant differences among all groups. Conclusion Variations in the centrifugation parameters and number of donors of saliva may influence C. albicans adhesion to denture base resins.
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Pós-graduação em Medicina Veterinária - FCAV
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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This experiment aimed to determine the influence of supplementation with protected fat on nematode infections in periparturient sheep or sheep in their final stage of pregnancy. Fifty Santa Ines ewes received 200 g of concentrate/animal/day, mineral salt and water and were grouped according to their weight, hematocrit and the number of eggs per gram of feces (EPG). Two diets were used: a control treatment and a treatment consisting of supplementation with 30 g/animal/day of protected fat in the concentrate. Three monthly evaluations were performed over a total of 84 days of testing. The variables analyzed were weight, body condition, EPG and coprocultures; blood tests were performed for the determination of packed cell volume, hemoglobin concentration and total plasma protein, and leukograms and eosinophil counts were performed. For weight and hemoglobin concentrations, interactions were observed between diet and collection date (P<0.05); body condition, total plasma protein, packed cell volume and total leukocytes did not differ statistically among treatments (P> 0. 05) but did differ by collection day (P <0.05). The EPG and eosinophil counts did not differ statistically by either diet or collection date (P>0.05). The genus Haemonchus was predominant, followed by the genera Cooperia, Trichostrongylus and Oesophagostomum. The protected fat did not decrease EPG and did not improve the blood parameters of infected sheep.
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Several studies have required Haematobia irritans (L.) raising in laboratory. The present study assessed two methods of inoculating immature forms of H. irritans to obtain adults. In 2007, 15 Nellore steers (Bos indicus) (L.) were used for the collection of feces free of anthelmintic treatment and flies to produce for eggs and larva. For method I, 30 eggs were incubated in square filter paper (5 × 5 cm) and deposited on bovine feces (500 g) where they were kept until hatching and spontaneous penetration of larvae (L1) into the fecal mass. After 24 h, eggs were analyzed under a stereoscope microscope (40×) for the number of larvae that instinctively penetrated the feces. In method II, larvae were obtained only by natural egg hatching. At birth, 30 larvae were collected and individually inoculated, directly onto the fecal plate by employing a moistened brush. The tests were carried out at controlled temperature (28˚C ± 2˚C) and saturated humidity (80%) until the emergence of flies with both methods. The number of emerged flies was considered in the result. Using method I, 276 (76.7%) flies emerged from 360 inoculated eggs, while using method II, 283 (78.6%) flies emerged from 360 inoculated larvae. There was no significant difference (P = 0.7821) between methods for the number of flies; however, the proportion between males and females by means of larva inoculation was different from 1:1 (P = 0.0146). Results indicated that both methods led to a satisfactory production of flies and egg inoculation provided an easier establishment.
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This study was conducted to evaluate the performance and characteristics of manure produced by Saanen goats with 90, 120 and 150 days of age and fed three diets, diets 1 = 80 % roughage (R) and 20 % concentrate (C), diets 2 = 60 % R and 40 % C, diets 3 = 40 % R and 60 % C. Were evaluated 24 goats (divided into three groups according to diet ) during the fattening stage, making up the collection of waste at 90, 120 and 150 days of age to quantify the mass of manure produced each day, beyond the content of N, P, K, Ca, Mg, Na, Fe, Cu, Zn and Mn. The animals were weighed weekly and during the collection of waste is evaluated the digestibility of DM, CP and NDF, in addition to the estimate of feed conversion and rate of waste. The best results of feed conversion coefficient of waste and the apparent digestibilities of DM, CP and NDF occurred at the age of 150 days and in animals fed diets containing higher proportions of concentrate. Insofar as the increased age of the animal and level of concentrate in the diets were verified higher production of faeces. All nutrients were also affected, with higher concentrations occurred in the feces of animals at 150 days old and fed with diet 40% R and 60% C.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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The Covenanters of South Carolina Collections consists of a "Sketch of the Covenanters of Rocky Creek, South carolina About 1750 to 1840" by Miss Mary Elder, 1886 as dictated to her by her father Mr. Matthew Elder, Yorkville, SC. Also included is an undated genealogical sketch of William Stinson (Stevenson) (1752-1809) who was a covenanter from Rocky Creek, SC and a "History of Fishing Creek Presbyterian Church by Mrs. Frank Hicklin" that was written ca. 1958. The Fishing Creek Presbyterian Church was organized ca. 1752.
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The Robert M. Ward Photograph Collection consists of approximately 400 prints and 900 negatives from the photographs that he took in the 1940s, 1950s, and 1960s. Most of the events and individuals pictured are from the York County, SC area and include the Latta House in York, South Carolina, downtown Rock Hill, parades, baseball, military, aviation, sports, and other York County buildings and scenes. Also included are photographs of Landsford Canal State Park, York Technical College, Winthrop University, Catawba Indian chieftain King Haigler’s memorial, Andrew Jackson State Park, Kings Mountain State Park, Hall of Fame Golfer Sam Sneed's visit to Rock Hill, SC, and Charleston, South Carolina. The collection also contains newspaper articles from the York Observer, dated from 1988 to 1992, which helped to identify some of the people and events in the pictures.
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The Jean Faut Papers consist primarily of memorabilia, photographs and images, scrapbooks, contracts, trophies, and correspondence related to Jean Faut's career in the All American Girls Professional Baseball League (AAGPBL) and reunions. Many of the images are digital-only copies of photographs, programs, scorecards, etc. from her scrapbooks and photographs produced during and after her playing career.