969 resultados para alternative splicing


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In 1985, the Higher Education Equity Program was introduced by the Australian Government to improve the participation of those persons from social groups traditionally under-represented within higher education. In 1990, the program was incorporated within A Fair Chance For All which provided more specific details of the government's desire for a system-wide approach to equity issues. One result has been the proliferation of access and equity programs conducted by universities around the country and aimed at redressing the disadvantage of potential students. The alleged success of these programs is based on greater participation in and graduation from Australian universities by individuals from targeted disadvantaged groups. The research reported here, however, would suggest that such programs are prone to co-opt the language of equity and social justice, dependent as they are on satisfying statistically-orientated program performance indicators in order to receive recurrent government funding. Further, the paper argues that success in achieving equity within Australian higher education will remain limited unless the structural arrangements that work to construct social inequalities in mainstream higher education are addressed.

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As with other types of flexible employment, a growing body of international research has found an association between temporary agency work and comparatively poor occupational safety and health outcomes. Temporary agency work has also been found to pose a serious challenge to regulatory regimes, including the activities of inspectors. Government agencies have responded to these challenges in a number of ways. This study examines a project undertaken in the Australian state of Queensland that sought to identify both particular problems and ways of resolving them. The focus of the project was to identify ‘non-regulatory’ solutions based on information collected through focus groups of agency and host employer representatives. However, while a number of policy interventions were identified that fitted this approach, the project also found that both agency firms and hosts believed additional regulatory controls were required. This paper assesses these findings in the context of broader research and policy debates about how to deal with the occupational safety and health problems posed by the global shift to more flexible work arrangements.

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Many cities around the world are looking for ways to reduce their per capita greenhouse gas emissions. The outward growth of cities from a central business district, typical of many cities around the world, is often seen as working against this goal and as unsustainable. This is especially the case in circumstances where this growth is not supported by the necessary infrastructure, often resulting in an increase in the use of private transport. However, alternative scenarios to contain the outward growth are being proposed. This paper provides a comparison of the energy demand and greenhouse gas emissions between typical detached outer-suburban housing currently being built in Australia's major cities and inner-city and -suburban apartments, which are increasingly seen as a legitimate alternative to the housing that is currently being built on our outer city fringes. By analysing the energy demand associated with the construction and operation of each housing type and for occupant travel it was found that the location of the housing and its size are the dominant factors determining energy use and greenhouse gas emissions. The findings from this analysis provide useful information for policy-makers in planning the development of our cities into the future, when faced with a growing population and an increasing need to minimise greenhouse gas emissions.

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Background: Complementary and alternative medicine (CAM) is common in patients with chronic disease such as diabetes mellitus. The primary objective of the study was to determine the overall prevalence and type of CAM use in individuals with diabetes mellitus (DM) in Western Sydney and to compare the prevalence and factors associated with CAM use with the literature.

Methods: A multicenter cross-sectional study was undertaken using a self-completed questionnaire distributed to patients with DM attending a public hospital and specialist endocrinology clinics in the region. The type of DM and pattern of CAM utilisation were analyzed.

Results: Sixty nine people responded to the questionnaire: age range of 18-75 years during a twelve week collection period. Overall, 32 respondents with diabetes were using some form of CAM, resulting in a utilisation rate of 46.3%. Twenty of the 32 CAM users used CAM specifically to treat their diabetes accounting for 28.9% of the respondent sample population. Multivitamins (40%), cinnamon, Co-enzyme q10 and prayer were the most frequently used CAM modalities. There was no significant difference between males and females, age range, income or diabetes complications between CAM and non-CAM users. (p values each > 0.05) The factor most significantly associated with CAM usage was being born overseas (p = 0.044).

Conclusions: Almost half the respondents (46.3%) used CAM: 28% used CAM specifically to treat their diabetes. Individuals born overseas were significantly more likely to use CAM than those born in Australia. Other factors such as age, gender, wealth and duration of living with diabetes were not associated with higher rate of CAM usage.

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This four-part paper is the 'highlights' of the 2009 Australian Technology Network conference, 'Assessment in Different Dimensions: a conference on teaching and learning in tertiary education', held at RMIT University, 19–20 November 2009.

The main theme of this paper is an exploration of how 'progressive and innovative' assessment techniques from other higher education providers might be used to 'progress and innovate' the assessment of Media and Communication students at Deakin University.

As lecturers and tutors have traditionally had almost total control over the learning and assessment environment, for most students, the approach to assessment has changed little. The arrival of 'new media', 'digital culture' and 'dispersed learning' threatens this stability and control. Students are now able to operate in a more open, collaborative, interactive and distributed manner, and this fact challenges many of the traditional perceptions about what constitutes a 'university experience' and what are now 'appropriate' assessment tasks (Crisp 2009).

Each of the four speakers will present a 'spotlight' initiative from the 2009 Australian Technology Network conference, describing how each assessment innovation might be useful to (a) confront our current ideas and values around what is 'good' and 'bad' assessment; (b) explain why some assessment myths are 'hard to shift'; and (c) suggest how these new approaches might be useful in the years to come.

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Over the past decades most goose populations have become increasingly dependent on agricultural crops during wintering and migration periods. The suitability of agricultural crops to support all nutritional requirements of migratory geese for the deposition of body stores has been questioned; feeding on agricultural crops may yield higher rates of fat deposition at the cost of reduced protein accretion due to an unbalanced diet. We compared amino-acid composition of forage, and investigated food-habitat use and dynamics and composition of body stores deposited by barnacle geese feeding on agricultural pasture and in natural salt marsh during spring migratory preparation. Overall content and composition of amino acids was similar among forage from both habitats and appeared equally suitable for protein accretion. There was no relationship between body composition of geese and their preferred food habitat. Fat and wet protein contributed with 67% and 33%, respectively, to body stores gained at a rate of 11 g/d throughout the one-month study period. We found no evidence of impaired protein accretion in geese using agricultural grassland compared to natural salt marsh. Our study supports the hypothesis that the expansion of feeding habitat by including agricultural grassland has played an important role in the recent growth of the East Atlantic flyway population of barnacle geese and other herbivorous waterbirds. Feeding refuges of improved grassland provide geese with an adequate diet for the deposition of body stores crucial for spring migration and subsequent reproduction, thereby alleviating the conflict with agriculture.

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An important area of recent research in forensic entomology has been the use of insect DNA to provide identification of insects for fast and accurate estimation of time since death. This requires DNA to be extracted efficiently and in a state suitable for use in molecular procedures, and then stored on a long-term basis. In this study, Whatman FTA™ cards were tested for use with the Calliphoridae (Diptera). In particular, testing examined their ability to effectively extract DNA from specimens, and store and provide DNA template in a suitable condition for amplification using the polymerase chain reaction (PCR). The cards provided DNA that was able to be amplified from a variety of life stages, and thus appears to be of sufficient quality and quantity for use in subsequent procedures. FTA cards therefore appear suitable for use with calliphorids, and provide a new method of extraction that is simple and efficient and allows for storage and transportation without refrigeration, consequently simplifying the handling of DNA in forensic entomological cases.

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This paper proposes a new research program and presents a current analysis of the potential of health information systems (HIS) to improve primary care delivery in rural Indonesia. A new HIS will be implemented to facilitate patient centred primary care and to support the interactions and collaborations between three types of participants including the patient, their doctors and pharmacist in Malang, Indonesia. A tetradic relationship between the new HIS and three participants (patient, doctors, and pharmacist) is examined through the lens of the actor network theory (ANT) with a view to form a new healthcare service delivery model for primary care providers in Indonesia. Based on this model, a network of primary care providers would share the patient medical records (PMR) and provide collaborative care programs to promote healthy life styles, prevent diseases, and to manage chronic disease care more effectively and efficiently.

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This article examines a conciliation narrative at King George Sound in Western Australia that originated in the early nineteenth century during an era of European exploration when explorers had fleeting meetings with the Mineng. This friendship narrative has been continually re-presented and inscribed by settlers and later by historians without its imperial power dynamic being critiqued. This essay attempts a genealogy of this friendship motif  as well as exploring the destablising of this narrative by writers and community members. This essay also suggests ways of decolonising this narrative and searches for alternative emotions from this frontier.

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Background Chromosomal translocations resulting in alternative fusions of the human TEL (ETV6) and JAK2 genes have been observed in cases of acute lymphoblastic leukemia and chronic myelogenous leukemia, but a full understanding of their role in disease etiology has remained elusive. In this study potential differences between these alternative TEL-JAK2 fusions, including their lineage specificity, were investigated.

Design and Methods TEL-JAK2 fusion types derived from both T-cell acute lymphoblastic leukemia and atypical chronic myelogenous leukemia were generated using the corresponding zebrafish tel and jak2a genes and placed under the control of either the white blood cell-specific spi1 promoter or the ubiquitously-expressed cytomegalovirus promoter. These constructs were injected into zebrafish embryos and their effects on hematopoiesis examined using a range of molecular approaches. In addition, the functional properties of the alternative fusions were investigated in vitro.

Results Injection of the T-cell acute lymphoblastic leukemia-derived tel-jak2a significantly perturbed lymphopoiesis with a lesser effect on myelopoiesis in zebrafish embryos. In contrast, injection of the atypical chronic myelogenous leukemia-derived tel-jak2a resulted in significant perturbation of the myeloid compartment. These phenotypes were observed regardless of whether expressed in a white blood cell-specific or ubiquitous manner, with no overt cellular proliferation outside of the hematopoietic cells. Functional studies revealed subtle differences between the alternative forms, with the acute lymphoblastic leukemia variant showing higher activity, but reduced downstream signal transducer and activator of transcription activation and decreased sensitivity to JAK2 inhibition. JAK2 activity was required to mediate the effects of both variants on zebrafish hematopoiesis.

Conclusions This study indicates that the molecular structure of alternative TEL-JAK2 fusions likely contributes to the etiology of disease. The data further suggest that this class of oncogene exerts its effects in a cell lineage-specific manner, which may be due to differences in downstream signaling.