Development of molecular approaches to assess the diversity and abundance of marine nematodes assemblages for assessment of Good Environmental Status of the estuarine and marine ecosystems

In Europe, the concerns with the status of marine ecosystems have increased, and the Marine Directive has as main goal the achievement of Good Environmental Status (GES) of EU marine waters by 2020. Molecular tools are seen as promising and emerging approaches to improve ecosystem monitoring, and have led ecology into a new era, representing perhaps the most source of innovation in marine monitoring techniques. Benthic nematodes are considered ideal organisms to be used as biological indicator of natural and anthropogenic disturbances in aquatic ecosystems underpinning monitoring programmes on the ecological quality of marine ecosystems, very useful to assess the GES of the marine environment. dT-RFLP (directed Terminal-Restriction Fragment Length Polymorphism) allows to assess the diversity of nematode communities, but also allows studying the functioning of the ecosystem, and combined with relative real-time PCR (qPCR), provides a high-throughput semi-quantitative characterization of nematode communities. These characteristics make the two molecular tools good descriptors for the good environmental status assessment. The main aim of this study is to develop and optimize the dT-RFLP and qPCR in Mira estuary (SW coast, Portugal). A molecular phylogenetic analysis of marine and estuarine nematodes is being performed combining morphological and molecular analysis to evaluate the diversity of free-living marine nematodes in Mira estuary. After morphological identification, barcoding of 18S rDNA and COI genes are being determined for each nematode species morphologically identified. So far we generated 40 new sequences belonging to 32 different genus and 17 families, and the study has shown a good degree of concordance between traditional morphology-based identification and DNA sequences. These results will improve the assessment of marine nematode diversity and contribute to a more robust nematode taxonomy. The DNA sequences are being used to develop the dT-RFLP with the ability to easily process large sample numbers (hundreds and thousands), rather than typical of classical taxonomic or low throughput molecular analyses. A preliminary study showed that the digest enzymes used in dT-RFLP for terrestrial assemblages separated poorly the marine nematodes at taxonomic level for functional group analysis. A new digest combination was designed using the software tool DRAT (Directed Terminal Restriction Analysis Tool) to distinguished marine nematode taxa. Several solutions were provided by DRAT and tested empirically to select the solution that cuts most efficiently. A combination of three enzymes and a single digest showed to be the best solution to separate the different clusters. Parallel to this, another tool is being developed to estimate the population size (qPCR). An improvement in qPCR estimation of gene copy number using an artificial reference is being performed for marine nematodes communities to quantify the abundance. Once developed, it is proposed to validate both methodologies by determining the spatial and temporal variability of benthic nematodes assemblages across different environments. The application of these high-throughput molecular approaches for benthic nematodes will improve sample throughput and their implementation more efficient and faster as indicator of ecological status of marine ecosystems.

The red swamp crayfish Procambarus clarkii in Europe: Impacts on aquatic ecosystems and human well-being

Procambarus clarkii is currently recorded from 16 European territories. On top of being a vector of crayfish plague, which is responsible for large-scale disappearance of native crayfish species, it causes severe impacts on diverse aquatic ecosystems, due to its rapid life cycle, dispersal capacities, burrowing activities and high population densities. The species has even been recently discovered in caves. This invasive crayfish is a polytrophic keystone species that can exert multiple pressures on ecosystems. Most studies deal with the decline of macrophytes and predation on several species (amphibians, molluscs, and macroinvertebrates), highlighting how this biodiversity loss leads to unbalanced food chains. At a management level, the species is considered as (a) a devastating digger of the water drainage systems in southern and central Europe, (b) an agricultural pest in Mediterranean territories, consuming, for example, young rice plants, and (c) a threat to the restoration of water bodies in north-western Europe. Indeed, among the high-risk species, P. clarkii consistently attained the highest risk rating. Its negative impacts on ecosystem services were evaluated. These may include the loss of provisioning services such as reductions in valued edible native species of regulatory and supporting services, inducing wide changes in ecological communities and increased costs to agriculture and water management. Finally, cultural services may be lost. The species fulfils the criteria of the Article 4(3) of Regulation (EU) No 1143/2014 of the European Parliament (species widely spread in Europe and impossible to eradicate in a cost-effective manner) and has been included in the “Union List”. Particularly, awareness of the ornamental trade through the internet must be reinforced within the European Community and import and trade regulations should be imposed to reduce the availability of this high-risk species.

Carbon in humic fractions of organic matter in soil treated with organic composts under mango cultivation.

Soil organic matter (SOM) plays a key role in maintaining the productivity of tropical soils, providing energy and substrate for the biological activity and modifying the physical and chemical characteristics that ensure the maintenance of soil quality and the sustainability of ecosystems. This study assessed the medium-term effect (six years) of the application of five organic composts, produced by combining different agro-industrial residues, on accumulation and chemical characteristics of soil organic matter. Treatments were applied in a long-term experiment of organic management of mango (OMM) initiated in 2005 with a randomized block design with four replications. Two external areas, one with conventional mango cultivation (CMM) and the other a fragment of regenerating Caatinga vegetation (RCF), were used as reference areas. Soil samples were collected in the three management systems from the 0.00-0.05, 0.05-0.10, and 0.10-0.20 m layers, and the total organic carbon content and chemical fractions of organic matter were evaluated by determining the C contents of humin and humic and fulvic acids. Organic compost application significantly increased the contents of total C and C in humic substances in the experimental plots, mainly in the surface layer. However, compost 3 (50 % coconut bagasse, 40 % goat manure, 10 % castor bean residues) significantly increased the level of the non-humic fraction, probably due to the higher contents of recalcitrant material in the initial composition. The highest increases from application of the composts were in the humin, followed by the fulvic fraction. Compost application increased the proportion of higher molecular weight components, indicating higher stability of the organic matter.

A vitalização agro-pecuária nas fronteiras da região amazônica: fronteira Brasil - Guiana Francesa.

1960

Análisis crítico a los agro(bio)combustibles

Industry, governments, and scientists that promote biofuels think these will become an alternative to oil, which is running out. These new fuels are supposed to help in containing climate change through greenhouse gas emission reduction, increasing farmers´ incomes, and promoting rural development. However, rigorous research and analyses undertaken by serious ecologists and social scientists suggests that the current boom in biofuels industry will be disastrous for farmers, the natural environment, the preservation of biodiversity, and particularly for poor consumers.

El agro costarricense en perspectiva histórica: un balance

Introducción Como parte de su interés hacia el campo de la historiografía, la Escuela de Historia de la Universidad Nacional, se abocó a la tarea de preparar durante 1988 un balance de los estudios históricos sobre el agro en Costa Rica, desde el periodo colonial hasta el presente. El momento era oportuno, por cuanto concluía una etapa de pesquisas individuales sobre aspectos específicos de la historia rural costarricense, al iniciarse el desarrollo de un programa colectivo de investigación  sobre esta ematica, asociada de  manera cada vez más  estrecha a la extensión y la docencia. La tarea de evaluar los estudios agrarios en Costa Rica, hoy resulta esencial por cuanto la llamada cuestión agraria es un factor básico en la actual coyuntura crítica, que tiende a introducir transformaciones sustanciales en la actividad productiva, la interacción social, las relaciones de poder y la vida cotidiana de los costarricenses.

AS TRANSFORMAÇÕES NA OCUPAÇÃO E UTILIZAÇÃO DO SOLO NO COMBINADO AGRO-URBANO DE BRASÍLIA I (BRASIL)

O presente artigo realiza movimento reflexivo acerca das transformações na utilização e ocupação do solo no Combinado Agro-urbano de Brasília I (CAUB) - Distrito Federal, Brasil. Dessa forma, objetivamos conhecer a comunidade local e analisar as transformações atuais no CAUB I. Em termos metodológicos o artigo foi desenvolvido pautado em reflexões de âmbito teórico e pesquisa da legislação acerca da ocupação territorial da área de estudo, acrescido de dados secundários. Depreende-se das primeiras observações sistematizadas que, anteriormente, o CAUB I caracterizava-se como um lugar para a produção agrícola onde a população local, morava, trabalhava, agregava renda e alimentação e se sentia importante dentro da comunidade. Atualmente, em decorrência do processo crescente de ocupação o sentido de pertencimento está se perdendo em meio ao interesse financeiro e à especulação imobiliária.

POLITICA ECONOMICA DE AJUSTE DEL AGRO Y SU EFECTO EN LOS GRUPOS SOCIALES DE GRANOS BASICOS.

Este artículo analiza la política económica de ajuste estructural, los mecanismos, e instrumentos más importantes de regulación y control, que afectan a los grupos sociales productores de granos básicos y sus efectos espaciales, enfatizando en la distribución del crédito.   Se considera la política económica como un conjunto de mecanismos des incentivadores que actúan sobre las características técnico-productivas, socio-productivas y espacial-productivas de los granos básicos. Esta diferenciación metodológica permite en el artículo visualizar efectos diferenciadores de grupos en la producción de granos en Costa Rica.   La política económica, en su ampliación instrumental se considera de aplicación homogénea, pero la realidad agraria territorial de los granos básicos es muy diferencial, se clasifican para el análisis en tres grupos y se define un grupo como clave, o más importante para el fomento o desestímulo de la política económica.  Dos periodos distintos se distinguen en la política hacia los granos básicos a partir de 1974, que los constituyen en el fomento de autosuficiencia alimentaria para después de 1984 empezar un desestímulo a los granos básicos. Los sectores sociales productores se diferencian entre empresarios arroceros y campesinos productores de maíz y frijol, donde el efecto de la política desincentivadora es distinto.   Se analiza el instrumento crediticio para ejemplarizar la política desincentivadora. Donde efectivamente se declara como una política sesgada por el apoyo crediticio del gran productor de arroz y por una segregación del pequeño productor.