Mine-waste pollution of Bear Butte Creek, Black Hills, South Dakota

Effluents leaving the Gilt Edge Mining properties in the Black Hills near Deadwood, South Dakota, were collected during April 1940. Field studies of these effluents and of the streams receiving them were made at the time and subsequently laboratory assays and analyses have been completed. ... Data from this particular case of mine waste pollution are presented here.

Water quality studies of the Delaware River with reference to shad migration

For some time pollution of the waters of the Delaware River by municipal and industrial wastes has been suspected of playing a major role in the decline of the shad fishery. Accordingly, studies were planned to ascertain whether any conditions of water quality caused by stream pollution and harmful or lethal to shad were existant in the waters of the Delaware River during the migration periods of the shad.

Toxicity of phenyl-mercuric lactate for fish

Phenyl-mercuric lactate is included in the pulp processing reagents by some paper mills to eliminate slime formation in the pulp. Small quantities of this chemical are added to the wet pulp in the beaters, particularly for the bactericidal action against Aerobacter aerogenes. Subsequently the mecurial is carried away in the wash waters. However, as the highly poisonous nature of many compounds of mercury is well known, questions have been raised concerning the pollution hazards created by phenyl-mercuric lactate in streams receiving effluents from mills using this substance.

Annual Report of the Game and Fisheries Department for the period Ist July, 1957 to 30th June, 1958

This report follows the now established practice of covering the financial year although, as before, for the sake of continuity and accuracy many of the statistics refer to the calendar year. This year has again been an outstanding one in the field of research on wildlife conservation problems, and culminated in one of the largest short-term biological projects yet carried out in the Protectorate. The latter involved the removal by shooting of nearly 500 hippo from badly over-grazed areas in the Queen Elizabeth National Park, but every carcass was examined by a team of scientists, a variety of scientific data collected, and the meat disposed of for local consumption. Not one animal was wasted.

Phylogenetic study of complete cytochrome b genes in musk deer (genus Moschus) using museum samples

As an endangered animal group, musk deer (genus Moschus) are not only a great concern of wildlife conservation, but also of special interest to evolutionary studies due to long-standing arguments on the taxonomic and phylogenetic associations in this group. Using museum samples, we sequenced complete mitochondrial cytochrome b genes (1140 bp) of all suggested species of musk deer in order to reconstruct their phylogenetic history through molecular information. Our results showed that the cytochrome b gene tree is rather robust and concurred for all the algorithms employed (parsimony, maximum likelihood, and distance methods). Further, the relative rate test indicated a constant sequence substitution rate among all the species, permitting the dating of divergence events by molecular clock. According to the molecular topology, M. moschiferus branched off the earliest from a common ancestor of musk deer (about 700,000 years ago); then followed the bifurcation forming the M. berezouskii lineage and the lineage clustering M. fuscus, M. chrysogaster, and M. leucogaster (around 370,000 years before present), interestingly the most recent speciation event in musk deer happened rather recently (140,000 years ago), which might have resulted from the diversified habitats and geographic barriers in southwest China caused by gigantic movements of the Qinghai-Tibetan Plateau in history. Combining the data of current distributions, fossil records, and molecular data of this study, we suggest that the historical dispersion of musk deer might be from north to south in China. Additionally, in our further analyses involving other pecora species, musk deer was strongly supported as a monophyletic group and a valid family in Artiodactyla, closely related to Cervidae. (C) 1999 Academic Press.

Modelling the long-term sustainability of indigenous hunting in Manu National Park, Peru: landscape-scale management implications for Amazonia

P> Widespread hunting throughout Amazonia threatens the persistence of large primates and other vertebrates. Most studies have used models of limited validity and restricted spatial and temporal scales to assess the sustainability. We use human-demographi

Ecological impacts of coastal aquaculture developments

The study presents the type and scale of any ecological change associated with coastal aquaculture development. These are enrichment, interaction with the food web, oxygen consumption, disturbance of wildlife and habitat destruction, interaction between escaped farmed stock and wild species, introduction and transfers, bioactive compounds (including pesticides and antibiotics), chemicals introduced via construction materials, and hormones and growth promoters.

The stripe-backed weasel Mustela strigidorsa: taxonomy, ecology, distribution and status

1. The stripe-backed weasel Mustela strigidorsa is one of the rarest and least-known mustelids in the world. Its phylogenetic relationships with other Mustela species remain controversial, though several unique morphological features distinguish it from congeners. 2. It probably lives mainly in evergreen forests in hills and mountains, but has also been recorded from plains forest, dense scrub, secondary forest, grassland and farmland. Known sites range in altitude from 90 m to 2500 m. Data are insufficient to distinguish between habitat and altitudes which support populations, and those where only dispersing animals may occur. 3. It has been confirmed from many localities in north-east India, north and central Myanmar, south China, north Thailand, north and central Laos, and north and central Vietnam. Given the limited survey effort, the number of recent records shows that the species is not as rare as hitherto believed. Neither specific nor urgent conservation needs are apparent.

Endosulfan: a hidden menace

A clean and healthy environment is paramount to human existence. While pesticide use has successfully sustained agricultural and food production in our lifetime as well as safeguarded human health by controlling insect pests, it has also caused many tragedies including population declines in our wildlife, fatalities in workers exposed to pesticides in its manufacture and use, and the increasing incidence of dreaded human illnesses such as cancer. A delicate balance should be achieved to mitigate the adverse impact of pesticide use to the environment and at the same time ensuring short- and long-term agricultural productivity. Endosulfan has been effectively used as a pesticide, but much evidence on its chronic and sub-lethal effects on humans and wildlife have been gathered in recent years. More research still needs to be done to determine its effects from long-term exposure at very low levels. Endosulfan is highly toxic to fish and other aquatic animals and, thus, not recommended for use in aquatic ecosystems. However, in some countries, it has been incorrectly used as a molluscicide in rice paddies, which could have an adverse impact on the rice-fish farming systems and on other surrounding aquatic ecosystems. It is clear that such practices should be stopped and users must strictly observe the recommended application methods. Agricultural productivity should be achieved with less pesticide by using integrated pest management programs which make use of biological, cultural, and physical control agents and lower doses of safer pesticide on a need only basis. The benefits of biotechnology should also be used to develop more effective and safer products and techniques. This is a valid approach and one that will require a unified and concerted effort among suppliers and users of pesticides in order to ensure that resources are used to our best advantage with minimal risk.

Crocodile farming: a multi-million dollar industry

Crocodiles have a long breeding life, which ranges from 25-30 years. There are 27 species and subspecies of crocodiles throughout the world, 18 of which are in danger of extinction, the rest being threatened with declining population due to overhunting and habitat destruction. Two known crocodile species exist in the Philippines: Crocodylus mindorensis (freshwater crocodile) and C. porosus (saltwater crocodile). Killing adult crocodiles, as is being done now, drastically reduces the potential population. Moreover, toxic wastes from mines, destruction of marshes and riverine habitats, and the conversion of their natural habitats for fishponds additionally threaten their populations. Estimates indicate that there are only about 100 Philippine crocodiles in the wild now. The Crocodile Farming Institute (CFI) was established in Palawan, in 1987, in order to save the crocodiles from extinction in the Philippines. It is now one of the components of the Palawan Wildlife and Conservation Center, and aims to conserve the 2 endangered species in the Philippines and also to develop and introduce a suitable crocodile farming technology that will help uplift the socio-economic well-being of the Filipino people. CFI believes in the potential of commercial utilization of crocodiles as a dollar-generating industry for the Philippines. It is a very profitable business and could be a multi-million dollar industry. A brief outline is given of the economic and marketing potentials of farming crocodiles in the Philippines.

Automated Detection of Concrete Columns from Visual Data

After earthquakes, licensed inspectors use the established codes to assess the impact of damage on structural elements. It always takes them days to weeks. However, emergency responders (e.g. firefighters) must act within hours of a disaster event to enter damaged structures to save lives, and therefore cannot wait till an official assessment completes. This is a risk that firefighters have to take. Although Search and Rescue Organizations offer training seminars to familiarize firefighters with structural damage assessment, its effectiveness is hard to guarantee when firefighters perform life rescue and damage assessment operations together. Also, the training is not available to every firefighter. The authors therefore proposed a novel framework that can provide firefighters with a quick but crude assessment of damaged buildings through evaluating the visible damage on their critical structural elements (i.e. concrete columns in the study). This paper presents the first step of the framework. It aims to automate the detection of concrete columns from visual data. To achieve this, the typical shape of columns (long vertical lines) is recognized using edge detection and the Hough transform. The bounding rectangle for each pair of long vertical lines is then formed. When the resulting rectangle resembles a column and the material contained in the region of two long vertical lines is recognized as concrete, the region is marked as a concrete column surface. Real video/image data are used to test the method. The preliminary results indicate that concrete columns can be detected when they are not distant and have at least one surface visible.

Waterborne exposure to PFOS causes disruption of the hypothalamus-pituitary-thyroid axis in zebrafish larvae

Thyroid hormones (THs) play an important role in the normal development and physiological functions in fish. Environmental chemicals may adversely affect thyroid function by disturbing gene transcription. Perfluorooctane sulfonate (PFOS), a persistent compound, is widely distributed in the aquatic environment and wildlife. In the present study, we investigated whether PFOS could disrupt the hypothalamic-pituitary-thyroid (HPT) axis. Zebrafish embryos were exposed to various concentrations of PFOS (0, 100, 200 and 400 mu g L-1) and gene expression patterns were examined 15 d post-fertilization. The expression of several genes in the HIPT system, i.e., corticotropin-releasing factor (CRF), thyroid-stimulating hormone (TSH), sodium/iodide symporter (NIS), thyroglobulin (TG), thyroid peroxidase (TPO), transthyretin (TTR), ioclothyronine deiodinases (Dio1 and Dio2) and thyroid receptor (TR alpha and TR beta), was quantitatively measured using real-time PCR. The gene expression levels of CRF and TSH were significantly up-regulated and down-regulated, respectively, upon exposure to 200 and 400 mu g L-1 PFOS. A significant increase in NIS and Diol gene expression was observed at 200 mu g L-1 PFOS exposure, while TG gene expression was down-regulated at 200 and 400 mu g L-1 PFOS exposure. TTR gene expression was down-regulated in a concentration-dependent manner. Up-regulation and down-regulation of TR alpha and TR beta gene expression, respectively, was observed upon exposure to PFOS. The whole body thyroxine (T-4) content remained unchanged, whereas triiodothyronine (T-3) levels were significantly increased, which could directly reflect disrupted thyroid hormone status after PFOS exposure. The overall results indicated that PFOS exposure could alter gene expression in the HPT axis and that mechanisms of disruption of thyroid status by PFOS could occur at several steps in the synthesis, regulation, and action of thyroid hormones. (C) 2009 Elsevier Ltd. All rights reserved.

Combined effects of polyfluorinated and perfluorinated compounds on primary cultured hepatocytes from rare minnow (Gobiocypris rarus) using toxicogenomic analysis

Polyfluorinated and perfluorinated compounds (PFCs) are used in numerous commercial products and have been ubiquitously detected in the environment as well as in the blood of humans and wildlife. To assess the combined effects caused by PFCs in mixtures, gene expression profiles were generated using a custom cDNA microarray to detect changes in primary cultured hepatocytes of rare minnows exposed to six individual PFCs (perfluorooctanoic acid, perfluorononanoic acid, perfluorodecanoic acid, perfluorododecanoic acid, perfluorooctane sulfonate, and 8:2 fluorotelomer alcohol) and four formulations of the PFCs mixtures. Mixtures as well as individual compounds consistently regulated a particular gene set, which suggests that these conserved genes may play a central role in the toxicity mediated by PFCs. Specifically, a number of genes regulated by the mixtures were identified in this study, which were not affected by exposure to any single component. These genes are implicated in multiple biological functions and processes, including fatty acid metabolism and transport, xenobiotic metabolism, immune responses, and oxidative stress. More than 80% of the altered genes in the PFOA- and PFOS-dominant mixture groups were of the same gene set, while the gene expression profiles from single PFOA and PFOS exposures were not as similar. This work contributes to the development of toxicogenomic approaches in combined toxicity assessment and allows for comprehensive insights into the combined action of PFCs mixtures in multiple environmental matrices. (C) 2009 Elsevier B.V. All rights reserved.

Protein Profiles in Zebrafish (Danio rerio) Embryos Exposed to Perfluorooctane Sulfonate

Perfluorooctane sulfonate (PFOS) is widely distributed and persistent in the environment and in wildlife, and it has the potential for developmental toxicity. However, the molecular mechanisms that lead to these toxic effects are not well known. In the present study, proteomic analysis has been performed to investigate the proteins that are differentially expressed in zebrafish embryos exposed to 0.5 mg/l PFOS until 192 h postfertilization. Two-dimensional electrophoresis coupled with mass spectrometry was employed to detect and identify the protein profiles. The analysis revealed that 69 proteins showed altered expression in the treatment group compared to the control group with either increase or decrease in expression levels (more than twofold difference). Of the 69 spots corresponding to the proteins with altered expression, 38 were selected and subjected to matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (TOF/TOF) analysis; 18 proteins were identified in this analysis. These proteins can be categorized into diverse functional classes such as detoxification, energy metabolism, lipid transport/steroid metabolic process, cell structure, signal transduction, and apoptosis. Overall, proteomic analysis using zebrafish embryos serves as an in vivo model in environmental risk assessment and provides insight into the molecular events in PFOS-induced developmental toxicity.