Taunton, Massachusetts 15 Minute Digital Raster Graphic

This layer is a digital raster graphic of the historic 15-minute USGS topographic map of the Taunton, Massachusetts quadrangle. The survey date (ground condition) of the original paper map is 1885, the edition date is September, 1893 and this map has a reprint date of 1940. A digital raster graphic (DRG) is a scanned image of a U.S. Geological Survey (USGS) standard series topographic map, including all map collar information. The image inside the map neatline is geo-referenced to the surface of the earth and fit to the Universal Transverse Mercator projection. The horizontal positional accuracy and datum of the DRG matches the accuracy and datum of the source map.

Conservation of the oligomeric state of native VDAC1 in detergent micelles.

The voltage-dependent anion-selective channel (VDAC) is an intrinsic β-barrel membrane protein located within the mitochondrial outer membrane where it serves as a pore, connecting the mitochondria to the cytosol. The high-resolution structures of both the human and murine VDACs have been resolved by X-ray diffraction and nuclear magnetic resonance spectroscopy (NMR) in 2008. However, the structural data are not completely in line with the findings that were obtained after decades of research on biochemical and functional analysis of VDAC. This discrepancy may be related to the fact that structural biology studies of membrane proteins reveal specific static conformations that may not necessarily represent the physiological state. For example, overexpression of membrane proteins in bacterial inclusion bodies or simply the extraction from the native lipid environment using harsh purification methods (i.e. chaotropic agents) can disturb the physiological conformations and the supramolecular assemblies. To address these potential issues, we have developed a method, allowing rapid one step purification of endogenous VDAC expressed in the native mitochondrial membrane without overexpression of recombinant protein or usage of harsh chaotropic extraction procedures. Using the Saccharomyces cerevisiae isoform 1 of VDAC as a model, this method yields efficient purification, preserving VDAC in a more physiological, native state following extraction from mitochondria. Single particle analysis using transmission electron microscopy (TEM) demonstrated conservation of oligomeric assembly after purification. Maintenance of the native state was evaluated using functional assessment that involves an ATP-binding assay by micro-scale thermophoresis (MST). Using this approach, we were able to determine for the first time the apparent KD for ATP of 1.2 mM.

The Sumner line of position furnished ready to lay down upon the chart by means of tables of simultaneous hour angle and azimuth of celestial bodies. Latitude 60æ N. to 60æ S.--declination 27æ N. to 27æ S.

"The plan of arrangement and scope of these tables, as well as the method of using them in navigation, were conceived by Mr. G.W. Littehales, C.E. Hydrographic office, Washington D.C."--Pref., p.iii.

If You Prohibit It, Bootleggers Will Come: A Mixed Method Study of the Landscape of College Basketball Betting

Over the past 25 years, betting on sports in the United States has grown dramatically with the majority of wagers being placed in illicit markets. Only 1% of an estimated 500 billion dollar sports betting industry in the United States is done legally. With this much at stake, the incentives to alter the outcome of the games are high. Corrupt gamblers can “fix” the match by offering payments to players to “throw the game,” that is, to predetermine the outcome in exchange for a payment (match fixing). This paper addresses whether match fixing in college basketball can be detected and contained with the current policies. A mixed method design will be used to identify possible “triggers” in Nevada Casino betting line movements that might warrant an investigation of cheating in the games. If match fixing can be detected at acceptable levels of probability, then current federal prohibitive law on sports betting might no longer be appropriate. Additionally, a survey will be administered to bettors in Nevada to analyze perceptions of the game’s integrity, further eroding the logic of current law.

Analysis of the free vibration of rectangular plates with central cut-outs using the discrete Ritz method

We present an analysis of the free vibration of plates with internal discontinuities due to central cut-outs. A numerical formulation for a basic L-shaped element which is divided into appropriate sub-domains that are dependent upon the location of the cut-out is used as the basic building element. Trial functions formed to satisfy certain boundary conditions are employed to define the transverse deflection of each sub-domain. Mathematical treatments in terms of the continuities in displacement, slope, moment, and higher derivatives between the adjacent sub-domains are enforced at the interconnecting edges. The energy functional results, from the proper assembly of the coupled strain and kinetic energy contributions of each sub-domain, are minimized via the Ritz procedure to extract the vibration frequencies and. mode shapes of the plates. The procedures are demonstrated by considering plates with central cut-outs that are subjected to two types of boundary conditions. (C) 2003 Elsevier Ltd. All rights reserved.