Alterations in energy metabolism, neuroprotection and visual signal transduction in the retina of parkinsonian, MPTP-treated monkeys

Parkinson disease is mainly characterized by the degeneration of dopaminergic neurons in the central nervous system, including the retina. Different interrelated molecular mechanisms underlying Parkinson disease-associated neuronal death have been put forward in the brain, including oxidative stress and mitochondrial dysfunction. Systemic injection of the proneurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to monkeys elicits the appearance of a parkinsonian syndrome, including morphological and functional impairments in the retina. However, the intracellular events leading to derangement of dopaminergic and other retinal neurons in MPTP-treated animal models have not been so far investigated. Here we have used a comparative proteomics approach to identify proteins differentially expressed in the retina of MPTP-treated monkeys. Proteins were solubilized from the neural retinas of control and MPTP-treated animals, labelled separately with two different cyanine fluorophores and run pairwise on 2D DIGE gels. Out of >700 protein spots resolved and quantified, 36 were found to exhibit statistically significant differences in their expression levels, of at least ±1.4-fold, in the parkinsonian monkey retina compared with controls. Most of these spots were excised from preparative 2D gels, trypsinized and subjected to MALDI-TOF MS and LC-MS/MS analyses. Data obtained were used for protein sequence database interrogation, and 15 different proteins were successfully identified, of which 13 were underexpressed and 2 overexpressed. These proteins were involved in key cellular functional pathways such as glycolysis and mitochondrial electron transport, neuronal protection against stress and survival, and phototransduction processes. These functional categories underscore that alterations in energy metabolism, neuroprotective mechanisms and signal transduction are involved in MPTPinduced neuronal degeneration in the retina, in similarity to mechanisms thought to underlie neuronal death in the Parkinson’s diseased brain and neurodegenerative diseases of the retina proper.

Experiencias y vivencias del estudiante de 4º de enfermería en el practicum

El proceso de aprendizaje que tiene lugar durante la formación práctico de los estudiantes genera algunos interrogantes: ¿de qué depende ese aprendizaje práctico? ¿Cómo se lleva a cabo?, ¿cuáles son las vivencias de los estudiantes durante esos meses de formación?, ¿cuáles son sus mayores dificultades de aprendizaje? etc. El objetivo de la investigación fue conocer y comprender cuáles fueron las vivencias y experiencias de los estudiantes de sus periodos de formación práctica ante la proximidad de convertirse en profesionales de la salud. Metodología: investigación cualitativa con método fenomenológico. Los datos se obtuvieron del análisis de las memorias reflexivas que los estudiantes de 4º de grado realizaron al finalizar las veinte semanas de prácticas correspondientes al Practicum IV. Conclusiones: a través de los testimonios de los estudiantes se identifican tres aspectos significativos: la necesidad de la práctica reflexiva, los condicionantes de las relaciones humanas en el aprendizaje y valor de los aspectos invisibles del cuidado.

Determinant factors of senior tourists’ length of stay

The current tendency to undertake more trips, but of shorter duration, throughout the year, has meant that the tourist industry has started to show greater interest in attracting those market segments that opt for more prolonged stays, as they are especially profitable. One of these segments is that of seniors. Given the aging demographic of the population worldwide, which is particularly noticeable in Spain, the object of this study is to identify the variables that determine the length of stay of Spanish seniors at their destination. The Negative Binomial model was adapted to the context of length of stay by Spanish seniors and the determinant factors identified were: age, travel purpose, climate, type of accommodation, group size, trip type and the activities carried out at the destination. This study is a contribution to this field from an empirical point of view, given the scarcity of studies of this type and their eminently descriptive character; as well as from a practical level, with interesting implications for the sector.

Percepción de las enfermeras de atención especializada sobre la prescripción enfermera

El objetivo de esta investigación fue determinar la percepción que sobre el concepto de “prescripción enfermera” tienen los profesionales enfermeros de atención especializada. Para ello se realizó un abordaje cualitativo mediante técnica Delphi, con la participación de 20 profesionales enfermeros que constituyeron el grupo de expertos. El período de estudio estuvo comprendido entre diciembre de 2008 y febrero de 2009 y se desarrolló en un hospital de tercer nivel de la Comunidad de Madrid. Se identificaron 17 actividades que fueron entendidas por el grupo de expertos como prescripción enfermera, siendo éstas agrupadas con arreglo a tres categorías: prescripción independiente, colaborativa y basándose en un protocolo. El grupo de expertos consideró que dichas actividades pueden ser desarrolladas mediante dos perfiles competenciales enfermeros: el perfil administrativo y el facultativo.

La gestión del conocimiento aplicada al desarrollo del departamento de investigación de enfermería en una institución de atención especializada

La Dirección de Operaciones y Enfermería del Hospital Universitario Fundación Alcorcón crea una plaza de Enfermera de Investigación y Formación, siendo este desempeño novedoso, tanto para el propio hospital, como para la mayor parte de los hospitales de la Comunidad de Madrid y no estando definidos los perfiles competenciales de puestos similares. Nos planteamos que, no habiéndose definido hasta ese momento las intervenciones de apoyo que dichas figuras ofertan, resulta difícil determinar el efecto que tienen sobre el personal al que brindan apoyo y asesoramiento, o conocer cuál es su repercusión en los resultados de salud de los pacientes, y por tanto, en la asistencia sanitaria que ofertan los hospitales. Por este motivo, se plantea la tesis que presenta como el objetivo general de definir el proceso de desarrollo competencial de una plaza de Enfermera de Investigación y Formación en una organización sanitaria de atención especializada, valorando la eficacia de su existencia en la mejora de la práctica asistencial enfermera, y por tanto, en la mejora de los resultados de salud de los pacientes. La obtención de los resultados se ha realizado mediante el desarrollo de un modelo de Gestión del Conocimiento basado en la Seguridad del Paciente, que permitía la valoración de las prácticas profesionales enfermeras y de los resultados en salud de los pacientes, asociando dichas evaluaciones a un diseño formativo en Seguridad del Paciente. Los resultados nos permiten concluir que el modelo desarrollado permitió la definición de las competencias de la Enfermera de Investigación y Formación, como figura administradora del modelo, y de las principales figuras impulsoras del mismo, consiguiendo difundir los contenidos en Seguridad del Paciente entre los colectivos receptores y permitiendo la valoración de aspectos de la práctica clínica enfermera y de los resultados de salud en los pacientes, que obtuvieron mejores indicadores tras el periodo de implementación del modelo de Gestión del Conocimiento en Seguridad del Paciente.

South America, 1807 (Image 2 of 7) (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Colombia Prima or South America : in which it has been attempted to delineate the extent of our knowledge of that continent, extracted chiefly from the original manuscript maps of ... Pinto, likewise fom those of João Joaquin da Rocha, João da Costa Ferreira ... Francisco Manuel Sobreviela &c. and from the most authentic edited accounts of those countries, digested & constructed by ... Louis Stanislas D'Arcy Delarochette. It was published by William Faden geographer to His Majesty and to His Royal Highness the Prince of Wales in June 4th, 1807. Scale [ca. 1:3,000,000]. This layer is image 2 of 7 total images of the eight sheet map, representing the northern portion of the map.The image inside the map neatline is georeferenced to the surface of the earth and fit to the South America Lambert Conformal Conic projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, roads, territorial boundaries, shoreline features, mines, tribes, and more. Relief shown by hachures. Includes notes.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Gulf of Mexico and Caribbean Sea Region, ca. 1715 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: A map of the West-Indies or the islands of America in the North Sea : with ye adjacent countries, explaning [sic] what belongs to Spain, England, France, Holland &c. also ye trade winds, and ye several tracts made by ye galeons and flota from place to place : according to ye newest and most exact observations, by Herman Moll, geographer. It was printed for Tho. Bowles in St. Pauls Church Yard and John Bowles at the Black Horse in Cornhill ca. 1715. Scale [ca. 1:4,300,000]. Covers the Gulf of Mexico and Caribbean Sea Region including parts of southern United States, Mexico, Central America, West Indies, and northern South America.The image inside the map neatline is georeferenced to the surface of the earth and fit to the North American Lambert Conformal Conic coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, territorial boundaries, shoreline features, and more. Relief shown pictorially. Includes also historical notes and insets.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Central America & Caribbean Region, 1858 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: A new map of tropical-America, north of the Equator : comprising the West-Indies, Central-America, Mexico, New Cranada [sic] and Venezuela by H. Kiepert. It was published by Dietrich Reimer in 1858. Scale [ca. 1:3,600,000].The image inside the map neatline is georeferenced to the surface of the earth and fit to the World Miller Cylindrical projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, roads, cities and other human settlements, territorial boundaries and colonial claims, shoreline features, and more. Relief shown by hachures and spot heights. Includes also text and inset map: Central part of the Mexican Republic on an enlarged scale, based upon the surveys published by A. v. Humboldt, v. Gerolt, Heller, Smith and the Sociedad Mejicana de Geografía y Estadística. Scale 1:1,000,000.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Central America & Caribbean Region, 1803 (Image 1 of 4) (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Chart of the West Indies and Spanish Dominions in North America, by A. Arrowsmith ; Jones, Smith & Co., sc. Beaufort Buildgs., Strand. It was published June 1st, 1803, by A. Arrowsmith, No. 24 Rathbone Place. Scale [ca. 1:2,775,525]. This layer is image 1 of 4 total images of the four sheet source map, representing the northeast portion of the map. Covers primarily Central America and the Caribbean region.The image inside the map neatline is georeferenced to the surface of the earth and fit to the World Miller Cylindrical projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, territorial boundaries, shoreline features, roads, mines, and more. Relief shown by hachures, depths shown by soundings. Includes notes.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Central America & Caribbean Region, 1803 (Image 2 of 4) (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Chart of the West Indies and Spanish Dominions in North America, by A. Arrowsmith ; Jones, Smith & Co., sc. Beaufort Buildgs., Strand. It was published June 1st, 1803, by A. Arrowsmith, No. 24 Rathbone Place in 1803. Scale [ca. 1:2,775,525]. This layer is image 2 of 4 total images of the four sheet source map, representing the southeast portion fo the map. Covers primarily Central America and the Caribbean region.The image inside the map neatline is georeferenced to the surface of the earth and fit to the World Miller Cylindrical projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, territorial boundaries, shoreline features, roads, mines, and more. Relief shown by hachures, depths shown by soundings. Includes notes.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Central America & Caribbean Region, 1803 (Image 3 of 4) (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Chart of the West Indies and Spanish Dominions in North America, by A. Arrowsmith ; Jones, Smith & Co., sc. Beaufort Buildgs., Strand. It was published June 1st, 1803, by A. Arrowsmith, No. 24 Rathbone Place. Scale [ca. 1:2,775,525]. This layer is image 3 of 4 total images of the four sheet source map, representing the southwest portion fo the map. Covers primarily Central America and the Caribbean region.The image inside the map neatline is georeferenced to the surface of the earth and fit to the World Miller Cylindrical projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, territorial boundaries, shoreline features, roads, mines, and more. Relief shown by hachures, depths shown by soundings. Includes notes.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Central America & Caribbean Region, 1803 (Image 4 of 4) (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Chart of the West Indies and Spanish Dominions in North America, by A. Arrowsmith ; Jones, Smith & Co., sc. Beaufort Buildgs., Strand. It was published June 1st, 1803, by A. Arrowsmith, No. 24 Rathbone Place. Scale [ca. 1:2,775,525]. This layer is image 4 of 4 total images of the four sheet source map, representing the northwest portion fo the map. Covers primarily Central America and the Caribbean region.The image inside the map neatline is georeferenced to the surface of the earth and fit to the World Miller Cylindrical projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, territorial boundaries, shoreline features, roads, mines, and more. Relief shown by hachures, depths shown by soundings. Includes notes.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

South America, 1779 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Carte du Pérou et Brésil sepl. de Tierre - Firme, de Guayana, et de la rivière des Amazones : ce qui fait la partie boréale de L'Amérique Meridiole., par le sr. d'Anville. It was published by P. Santini, Chez M. Remondini in 1779. Scale [ca. 1:6,500,000]. Covers the northern part of South America. Map in French. The image inside the map neatline is georeferenced to the surface of the earth and fit to a non-standard 'World Sinusoidal' projection with the central meridian at 58.000000 degrees west. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, territorial boundaries, shoreline features, and more. Relief is shown pictorially. This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection and the Harvard University Library as part of the Open Collections Program at Harvard University project: Organizing Our World: Sponsored Exploration and Scientific Discovery in the Modern Age. Maps selected for the project correspond to various expeditions and represent a range of regions, originators, ground condition dates, scales, and purposes.

Cuba, 1835 (Image 1 of 6) (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Carta geogro. topográfica de la isla de Cuba : dedicanla a la Reyna Nuestra Señora Doñ a Isabel II, El Teniente General Conde de Cuba y la Comisión de Gefes y Oficiales Militares y de Agrimensores Públicos que la levantó y formó de su orden en los años de 1824 á 1831/Do. Estruch lo grabó en Barcelona 1835 ; Carlos Roca lo dibujó. It was published in 1835. Scale [ca. 1:325,000]. This layer is image 1 of 6 total images of the six sheet source map. Map in Spanish.The image inside the map neatline is georeferenced to the surface of the earth and fit to the World Mercator project coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, roads, shoreline features, and more. Relief shown by hachures, depths by soundings. Includes also indexes, table of distances, statistical tables, and insets: "Plano de la ciudad y puerto de La Habana" [ca. 1:16.000]. -- "Plano de la ciudad y bahía de Sn. Carlos de Matanzas" [ca. 1:14.500]. -- "Ciudad de Sta. María del Puerto Principe" [ca. 1:16.000]. -- "Plano de la ciudad de Santiago de Cuba" [ca. 1:16.000] -- "Plano de la bahía de Cuba" [ca. 1:48.000]. -- "Plano de la ciudad de Trinidad" [ca. 1:16.000]. -- "Puerto de Casilda" [ca. 1:43.000].This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.