Structures of the noncovalent complexes of human and bovine prothrombin fragment 2 with human PPACK-thrombin

Both human and bovine prothrombin fragment 2 (the second kringle) have been cocrystallized separately with human PPACK (D-Phe-Pro-Arg)-thrombin, and the structures of these noncovalent complexes have been determined and refined (R = 0.155 and 0.157, respectively) at 3.3-Å resolution using X-ray crystallographic methods. The kringles interact with thrombin at a site that has previously been proposed to be the heparin binding region. The latter is a highly electropositive surface near the C-terminal helix of thrombin abundant in arginine and lysine residues. These form salt bridges with acidic side chains of kringle 2. Somewhat unexpectedly, the negative groups of the kringle correspond to an enlarged anionic center of the lysine binding site of lysine binding kringles such as plasminogens K1 and K4 and TPA K2. The anionic motif is DGDEE in prothrombin kringle 2. The corresponding cationic center of the lysine binding site region has an unfavorable Arg70Asp substitution, but Lys35 is conserved. However, the folding of fragment 2 is different from that of prothrombin kringle 1 and other kringles: the second outer loop possesses a distorted two-turn helix, and the hairpin β-turn of the second inner loop pivots at Val64 and Asp70 by 60°. Lys35 is located on a turn of the helix, which causes it to project into solvent space in the fragment 2-thrombin complex, thereby devastating any vestige of the cationic center of the lysine binding site. Since fragment 2 has not been reported to bind lysine, it most likely has a different inherent folding conformation for the second outer loop, as has also been observed to be the case with TPA K2 and the urokinase kringle. The movement of the Val64-Asp70 β-turn is most likely a conformational change accompanying complexation, which reveals a new heretofore unsuspected flexibility in kringles. The fragment 2-thrombin complex is only the second cassette module-catalytic domain structure to be determined for a multidomain blood protein and only the third domain-domain interaction to be described among such proteins, the others being factor Xa without a Gla domain and Ca2+ prothrombin fragment 1 with a Gla domain and a kringle. © 1993 American Chemical Society.

Natural fiber reinforced unsaturated polyester composites - An approach on compression molding

This work has been performed at Tapetes Sao Carlos-Brazil with the cooperation of the DaimlerChrysler Research Center Team in Ulm - Germany. The objective of the present paper is to report the results obtained with natural fiber reinforced unsaturated polyester (UP) composites, concerning surface quality measurements. The fibers that have been chosen for this work were sisal and curaua. The samples were produced by compression molding technique and afterwards submitted to three different tests, namely: a) thermal aging; b) water absorption and c) artificial weathering. The surface parameters measured before and after the tests were gloss, haze, short and long-waviness. The results have shown that after the tests there is a high loss of gloss, a high increase in haze, and a high increase in short and long-waviness as well. Curaua reinforced composites had a slightly better behavior when compared with sisal reinforced composites. The effect of the presence of filler and the addition of thermoplastic polyester (TP) on the material behavior has not been evidently detected. This result shows that the conventional technology/methods applied to UP-Fiberglass systems cannot be transferred to natural fibers without any modification. The fiber-matrix interaction and its response to the presence of additives must be fully understood before a successful processing route can be developed for painted natural fibers reinforced UP. Copyright © 2001 Society of Automotive Engineers, Inc.

Panoramic view of the occurrence of Yersinia species other than Y. pestis in Brazil

Data on the occurrence of Yersinia species, other than Y. pestis in Brazil are presented. Over the past 40 years, 767 Yersinia strains have been identified and typed by the National Reference Center on Yersinia spp. other than Y. pestis, using the classical biochemical tests for species characterization. The strains were further classified into biotypes, serotypes and phagetypes when pertinent. These tests led to the identification of Yersinia cultures belonging to the species Y. enterocolitica, Y. pseudotuberculosis, Y. intermedia, Y. frederiksenii and Y. kristensenii. Six isolates could not be classified in any of the known Yersinia species and for this reason were defined as Non-typable (NT). The bio-sero-phagetypes of these strains were diverse. The following species of Yersinia were not identified among the Brazilian strains by the classical phenotypic or biochemical tests: Y. aldovae, Y. rhodei, Y. mollaretti, Y. bercovieri and Y. ruckeri. The Yersinia strains were isolated from clinical material taken from sick and/or healthy humans and animals, from various types of food and from the environment, by investigators of various Institutions localized in different cities and regions of Brazil.

Evaluation of neighborhood impacts caused by companies of the high-tech industrial cluster of São Carlos, SP, Brazil

The purpose of this study was to develop a methodology for evaluating neighborhood impacts using a Geographic Information System (GIS) and to apply the procedures to the companies of the High-Technology Industrial Cluster of São Carlos. To this end, an evaluation was made of the neighborhood impacts on the physical environment, urban components, quality of life, and urban infrastructure using impact matrices, and the impacts were assigned scores according to type, order, magnitude and duration. Fifty one companies were examined based on data provided by the companies themselves and on field surveys. The impacts are represented spatially in proportional symbols maps, based on the spatial distribution of the companies in the urban area of the city of São Carlos and the areas of influence of each company. The application of the proposed methodology served to validate it and indicated that the neighborhood impacts caused by the companies of this study are related to each company's type of activity, its size, and its occupation of the area. © 2008 Journal of Urban and Environmental Engineering (JUEE). All rights reserved.

The top 20 multinationals in Chile in 2010: retail, forestry and transport lead the international expansion

Incluye bibliografía

Potential output in Latin America: a standard approach for the 1950-2002 period

Includes bibliography

A First approach on the new transmission system in northern Brazil

This article shows an analysis of the electrical parameters of a three-phase transmission line section using a relatively new design for steel towers characterized by 280 meters high. These main features, the height of the line conductors and distance between them, are intrinsically related to the longitudinal and transversal parameters of the line. By this means, an analysis was carried out in order to show the electrical variations between a transmission line using the new technology and a conventional 440-kV line through a wide range of frequencies and a variable soil resistivity. © 2012 The Institution of Engineering and Technology.

A comparative study of the physical properties of conventional and Grander-modified adhesive systems

Purpose: The objective of this study was to evaluate the influence of the Grander technology in reducing the surface tension and contact angle of a self-etch adhesive system. Methods: Distilled water and Xeno III self-etch adhesive system (Dentsply) were modified by physical contact with the Flexible unit Grander system to revitalize water, for 48 hours, resulting in four groups: Group 1 - Xeno III under normal conditions; Group 2 - Xeno III modified by Grander; Group 3 - distilled water under normal conditions; Group 4 - distilled water system modified by the Grander system;. Surface tension and contact angle (dentin substrate and titanium plate) of the adhesive system and water in normal and Grander-modified conditions was measured with a goniometer. Data were analyzed by ANOVA and Tukey test (5%). Results: Grander technology reduced the surface tension of water but not of the adhesive system Xeno III. The contact angle property of the adhesive ystem was not affected. Conclusion: Grander technology did not interfere with the surface tension and contact angle physical properties of the Xeno III self-etch adhesive system. Copyright: © 2011 Gonçalves et al.;.

Introduction of a non-conventional solution for Amazon transmission system: Analysis of the electric aspects

This article shows an analysis of the longitudinal electric parameters of a three-phase transmission line/section using a 280-meter high steel tower. This characteristic, the height of the line conductors and distance between them, are intrinsic related to the longitudinal and transversal parameters of the line. By this means, an accurate study was carried out in order to show the electric variations between a transmission line using the new technology and a three-phase conventional 440 kV line for a wide range of frequencies and a variable soil resistivity. In addition, by using a digital line model, simulations are carried out in time domain to analyze critical overvoltage transients on the studied line. © 2011 IEEE.

Estudo da comunicação de um compensador série para rede de distribuição com o centro de controle utilizando tecnologia GPRS

Pós-graduação em Engenharia Elétrica - FEIS

Influência do mercado na adoção de tecnologias e viabilidade econômica da cultura do tomate de mesa, município de Bragança Paulista - SP

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Possibilidades e limites da aprendizagem baseada em problemas no ensino médio

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Report on the production of trade statistics in the Caribbean region

Includes bibliography

O Centro Estadual de Educação de Jovens e Adultos de Presidente Prudente (CEEJA-PP) significa realmente espaço formativo?

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

An assessment of the economic impact of climate change on the water sector in the Turks and Caicos Islands

The best description of water resources for Grand Turk was offered by Pérez Monteagudo (2000) who suggested that rain water was insufficient to ensure a regular water supply although water catchment was being practised and water catchment possibilities had been analysed. Limestone islands, mostly flat and low lying, have few possibilities for large scale surface storage, and groundwater lenses exist in very delicate equilibrium with saline seawater, and are highly likely to collapse due to sea level rise, improper extraction, drought, tidal waves or other extreme event. A study on the impact of climate change on water resources in the Turks and Caicos Islands is a challenging task, due to the fact that the territory of the Islands covers different environmental resources and conditions, and accurate data are lacking. The present report is based on collected data wherever possible, including grey data from several sources such as the Intergovernmental Panel on Climate Change (IPCC) and Cuban meteorological service data sets. Other data were also used, including the author’s own estimates and modelling results. Although challenging, this was perhaps the best approach towards analysing the situation. Furthermore, IPCC A2 and B2 scenarios were used in the present study in an effort to reduce uncertainty. The main conclusion from the scenario approach is that the trend observed in precipitation during the period 1961 - 1990 is decreasing. Similar behaviour was observed in the Caribbean region. This trend is associated with meteorological causes, particularly with the influence of the North Atlantic Anticyclone. The annual decrease in precipitation is estimated to be between 30-40% with uncertain impacts on marine resources. After an assessment of fresh water resources in Turks and Caicos Islands, the next step was to estimate residential water demand based on a high fertility rate scenario for the Islands (one selected from four scenarios and compared to countries having similar characteristics). The selected scenario presents higher projections on consumption growth, enabling better preparation for growing water demand. Water demand by tourists (stopover and excursionists, mainly cruise passengers) was also obtained, based on international daily consumption estimates. Tourism demand forecasts for Turks and Caicos Islands encompass the forty years between 2011 and 2050 and were obtained by means of an Artificial Neural Networks approach. for the A2 and B2 scenarios, resulting in the relation BAU>B2>A2 in terms of tourist arrivals and water demand levels from tourism. Adaptation options and policies were analysed. Resolving the issue of the best technology to be used for Turks and Caicos Islands is not directly related to climate change. Total estimated water storage capacity is about 1, 270, 800 m3/ year with 80% capacity load for three plants. However, almost 11 desalination plants have been detected on Turks and Caicos Islands. Without more data, it is not possible to estimate long term investment to match possible water demand and more complex adaptation options. One climate change adaptation option would be the construction of elevated (30 metres or higher) storm resistant water reservoirs. The unit cost of the storage capacity is the sum of capital costs and operational and maintenance costs. Electricity costs to pump water are optional as water should, and could, be stored for several months. The costs arising for water storage are in the range of US$ 0.22 cents/m3 without electricity costs. Pérez Monteagudo (2000) estimated water prices at around US$ 2.64/m3 in stand points, US$ 7.92 /m3 for government offices, and US$ 13.2 /m3for cistern truck vehicles. These data need to be updated. As Turks and Caicos Islands continues to depend on tourism and Reverse Osmosis (RO) for obtaining fresh water, an unavoidable condition to maintaining and increasing gross domestic product(GDP) and population welfare, dependence on fossil fuels and vulnerability to increasingly volatile prices will constitute an important restriction. In this sense, mitigation supposes a synergy with adaptation. Energy demand and emissions of carbon dioxide (CO2) were also estimated using an emissions factor of 2. 6 tCO2/ tonne of oil equivalent (toe). Assuming a population of 33,000 inhabitants, primary energy demand was estimated for Turks and Caicos Islands at 110,000 toe with electricity demand of around 110 GWh. The business as usual (BAU), as well as the mitigation scenarios were estimated. The BAU scenario suggests that energy use should be supported by imported fossil fuels with important improvements in energy efficiency. The mitigation scenario explores the use of photovoltaic and concentrating solar power, and wind energy. As this is a preliminary study, the local potential and locations need to be identified to provide more relevant estimates. Macroeconomic assumptions are the same for both scenarios. By 2050, Turks and Caicos Islands could demand 60 m toe less than for the BAU scenario.