Avaliação dos efeitos citotóxicos, genotóxicos e mutagênicos do fipronil, após fotodegradação, utilizando Allium cepa como organismo teste

Compounds released into the environment can induce genetic alterations in living organisms. A group of chemicals that shows proven toxicity is the pesticides, and the insecticides are the most harmful. The insecticides of the family phenylpyrazole have wide application both in agriculture and in homes. Fipronil, an insecticide of this chemical group, is widely used in various cultures and in homes, mainly for fighting fleas and ticks on dogs and cats. The use of fipronil may represent a risk to man and the environmental health, since this pesticide can potentially induce cell death, regardless of cell type. Fipronil, when in contact with the environment, can undergo various degradation processes, including photodegradation. The toxic effect of one of its metabolites derived from photodegradation, sulfone-fipronil, is approximately 20 fold as great as fipronil itself. The A. cepa test system was used to evaluate cytotoxic, genotoxicity and mutagenic effects of fipronil before and after phptodegradation. Seeds of Allium cepa were subjected to solutions of fipronil, pre-exposed or not exposed to degradation by sunlight. The germination tests were conducted both under the effect of light and in the dark. We evaluated the cumulative potential of this insecticide using 48 and 72-hours recovery tests. The results showed that when fipronil was previously exposed to the sun, it presented a greater genotoxic and mutagenic potential, showing that the metabolites formed by photodegradation can show more harmfull effects

Arquitetura Orientada a Serviços baseada na Tecnologia Jini: um caso de teste em Computação Ubíqua

T he people’s daily lives are surrounded by computing devices, with increasing resources (sensors) and, with increasing processing. How these devices communicate is still not natural and This retards the growth of Ubiquitous Computing. This paper presents a way in which these devices can communicate using Jini technology and concepts of Service Oriented Architecture, applying these concepts in a test case of Ubiquitous Computing. To conduct the test case was constructed a fictitious system for management of a soccer championship, where users can interact with each other and with the system in a simplified way, have access to data in real time of the championship during the event. This communication is performed by services built using Jini technology, which were based on key SOA concepts, such as modularity and reusability

O Paraguai na agenda externa brasileira e a temática de Itaipu: 2003-2010

Pós-graduação em Relações Internacionais (UNESP - UNICAMP - PUC-SP) - FFC

Investigação do envolvimento de subtipos de adrenoceptores α1 no efeito anti-imobilidade da imipramina no teste de suspensão pela cauda

Pós-graduação em Ciências Biológicas (Farmacologia) - IBB

Organização e funcionamento do atendimento educacional especializado na educação infantil: estudo de caso

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Padronização do teste dot blotting e comparação entre testes de diagnóstico de brucelose em bovinos

Pós-graduação em Medicina Veterinária - FCAV

Tempo Ideal para a coleta do sopro no teste respiratório com isótopos estáveis (13C) para o diagnóstico do Helicobacter pylori

A study was conducted to determine the optimal time for collecting the breath examination urea breath marked with the stable isotope 13C. We selected patients before undergoing the examination of endoscopy at the Endoscopy Section of the University Hospital of Botucatu - SP. A screening was performed to determine which patients wanted and could participate. Before performing endoscopy basal sample was collected from the patient and then the labeled urea ingested. The blows were collected in double every 2.5 minutes until an interval of 30.0 minutes after were collected every 5.0 minutes until the time of 45.0 minutes . The samples were analyzed in a mass spectrometer for isotope ratio, located in the center of Stable Isotopes, Institute of Biosciences, UNESP - Botucatu campus. The data were studied and arranged in the form of graphics to better interpretation of results. Based on the obtained results it was determined that a standby time of 15.0 minutes to collect the wind is sufficient for accurate diagnosis and effective

Emulação, teste e validação de conceitos e conteúdos

This article aims to describe the proposed development of an emulator application for digital TV that operates on a local computer network, to have it as a testing environment for students and researchers. The emulator application is presented as a useful tool to reduce validation costs of applications developed for digital TV.

A temática da violência escolar na formação docente inicial: das lacunas existentes às discussões necessárias

This paper emerged from a reflection on the troubles afflicting and shocked teachers, especially early in his career, among which stands out both in studies and in reports propagated by the teachers themselves: the violence manifested in and out of classrooms. Given this finding and the obvious need for overcoming beyond ephemeral measures, this research outlines two equally important goals: a) incite the discussion of violence at the time of initial teacher training as well as the emergence of new studies concerning this subject in academic research and b) transgress the tradition of research that consists in recommending prescriptions to be applied about how banning school violence, disregarding the peculiarities of each context. These objectives are derived from following question: which strategies are developed in undergraduate courses that aim to preparing of future teachers to deal with the phenomenon of violence in elementary school? Intending to answer this question and, therefore, contemplate the objectives outlined we opted for specialized bibliographic material analysis, systematically selected as the method. As a main result, it was found that the discussion of the theme of school violence in the initial teacher training is something still scarce in practice and incipient in the sphere of research. Therefore, we emphasize the importance of strengthening of this discussion so that when they get into career teaching, future teachers feel adequately subsidized up to face the everyday challenges that may manifest over the profession.

Suposição de normalidade e gestão de risco: uma aplicação do var paramétrico via teste de aderência

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Programa informativo sobre deficiência mental e inclusão: mudanças nas atitudes socias de crianças sem deficiência

This article presents part of a study that analyzed the concepts, feelings and attitudes of children without disabilities about mental retardation and inclusion and evaluated the effects of an informative program that deals with the issue. The study included forty children from two first grade classrooms in a public school in Marília-SP. One classroom participated as a control group. All children underwent pre and post tests in the form of interviews on the subject and a scale of children's social attitudes towards inclusion was applied. The experimental group participated in the informative program, composed of thirteen weekly meetings, in which the limitations and possibilities of people with mental retardation, specialized care, their schooling and family and social aspects, were discussed, using various educational and recreational strategies. The data collected in the interviews were categorized and content analysis was conducted. With the scale, individual scores were obtained. Statistical calculations were performed to verify the significance of differences between groups. In this paper we discuss the data obtained with the scale which were crossed with interview data. The results of the interviews and the scale indicated several changes in children's attitudes towards inclusion, but relations between many of these data could not be statistically confirmed. These results indicate the importance of expanding the research on the relationship between the phenomena presented.

Patógenos periodontais: comparação entre cultura bacteriana e PCR em tempo real para teste diagnóstico

The correct distinguishment of microorganisms involved in the periodontal disease pathogen, it is important in the understanding of its progression and adequate treatment planning. Considering this fact, some molecular methods of identification and quantification were developed and are extremely sensitive and precise in the characterization of different bacteria species. The present study aimed to realize a literature review, including studies that realized a comparative analysis between bacterial culture and real time PCR methods in the identification of pathogens. The bacterial culture method can possibly identify new microorganisms and realize antibiotics sensitivity tests. The real time PCR is a microbiologic test that identifies and quantifies bacterial species, through gene amplification of predetermined DNA fragments, with high sensitivity and specificity, and need a shorter operation time of the operator when compared to the bacterial culture method. In this way, to determine a specific diagnostic test, should be considered not only its precision in the identification of microorganisms, but the cost-benefit relationship as well.

Confiabilidade do teste de identificação de transtornos devido ao uso de álcool (AUDIT) em adolescentes

The objective of this study was to estimate the reliability of the alcohol use disorders identification test (AUDIT) applied to adolescents. Evaluations were performed with 141 students of ages ranging from 14 to 17 years (14.93±0.62). The internal consistency of the instrument was estimated by Cronbach’s alpha coefficient and its reproducibility by weighted Kappa statistics by score and by a reliability interval of 95%. The AUDIT presented excellent internal consistency (α=0.80, rinter-item=0.29). Most questions present reproducibility ranked as “good”. It is concluded that the AUDIT is a reliable instrument to survey information about alcohol use in adolescents.

Reação postural de pacientes com doença de Parkinson e idosos sadios no teste de retropulsão instrumentado

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Respostas da frequência cardíaca ao teste de 1RM e da modulação autonômica da frequência cardíaca no período de recuperação em indivíduos com fatores de risco para doenças cardiovasculares

Pós-graduação em Desenvolvimento Humano e Tecnologias - IBRC